terclim by ICS banner
IVES 9 IVES Conference Series 9 A NEW STRATEGY AND METHODOLOGY FOR THE CHARACTERIZATION OF POLYPHENOLS IN FINING PRECIPITATE

A NEW STRATEGY AND METHODOLOGY FOR THE CHARACTERIZATION OF POLYPHENOLS IN FINING PRECIPITATE

Abstract

Polyphenols are secondary metabolite widely distributed in plant kingdom such as in fruits, in grapes and in wine. During the winemaking process, polyphenols are extract from the skin and seed of the berries. Fining is an important winemaking step just before bottling which has an impact on wine stabi-lization and clarification. Most the time, fining agent are animal or vegetal protein while some of them can be synthetic polymer like PVPP or natural origin like bentonite. The aim of this study was better understand colloidals phenomenons involved in fining process and determine how polyphenols content and composition are impact by finning.

Different type and composition of fining agent were used during the investigation on each wine. Some of them were based on animal proteins, vegetal proteins, PVPP or bentonite alone while some mix with PVPP and vegetal proteins, PVPP and animal protein, and a mix with PVPP, vegetal proteins and bentonite were also used. On the wine before and after fining monomeric and total anthocyanins, monomeric, dimeric and total tannins, mDP (mean degree of polymerization), flavonol and phenolic acids content were measured. However in order to determine more precisely the content and composition of polyphenol lost during fining, a new strategy have been develop based on the re-solubilisation of the fining precipitate using some organic solvent.

For these experiments, this new method highlight some drastic differences between fining agents. Indeed, depending of the fining agent the amount and the composition of the polyphenols present in the fining precipitate change. For example, some fining agent don’t remove anthocyanins while some other precipitate mainly the p-coumarolylated anthocyanins. Moreover, important differences are also observed for condensed tannins according to the nature of the fining agent. Indeed, fining agent without PVPP were able to precipitate monomeric or dimeric condensed tannins. Moreover, some fining agent are more selective of oligomeric tannins while some has tendency to precipitate tannins with higher mDP.

This new methodology allow a more precise and clear identification of the polyphenol precipitated by fining agent and will help to better understand impact of the fining the organoleptic properties of the wine. Similarly, a better characterization of the fining precipitate will also help better understanding the colloidal structure of the wine.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Gauthier Lagarde¹, Manon ferreira¹, Sandra Vanbrabant¹, Soizic Lacampagne¹, Arnaud Massot², Virginie Moine², Pierre-Louis Teissedre¹ and Michael Jourdes*,1

1. Université de Bordeaux, Bordeaux INP, Bordeaux Sciences Agro, UMR 1366 OENOLOGIE, ISVV, 33140 – Villenave d’Ornon
2. Biolaffort, 11 Rue Aristide Berges, 33270 Floirac, Gironde, France

Contact the author*

Keywords

Fining, Precipitate, Condensed tannins, Anthocyanin

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Molecular approaches for understanding and modulating wine taste

Wine consumers generally demand wines having a perception of softer tannins and less ripe, having a heaviness and richness on palate (full-body wine) with a limpid and stable color. However, polyphenol
(tannins)-rich wines have been also correlated with unpleasant taste properties such as astringency and
bitterness when perceived at high intensities. Modulating these unpleasant properties could be important for consumer’s approval of wines.

EVALUATION OF THE OENOLOGICAL POTENTIAL OF NEW RESISTANT VARIETIES MEETING TYPICAL BORDEAUX CHARACTERISTICS

Varietal innovation is a major lever for meeting the challenges of the agro-ecological transition of vi-neyards and their adaptation to climate change. To date, selection work has already begun in the Bordeaux region through the Newvine project. The aim of this project is to create new vine varieties with resistance to mildew and powdery mildew, adapted to the climatic conditions of the Bordeaux region and enabling the production of wines that are in line with consumer tastes and the expected typicity of Bordeaux wines.

EFFECT OF FERMENTATION TEMPERATURE GRADIENT AND SKIN CONTACT ON ESTER AND THIOL PRODUCTION AND TROPICAL FRUIT PERCEPTION IN CHARDONNAY WINES

Wines with tropical fruit aromas have become increasingly more available1,2. With increased availability of different wine styles, it has become important to understand the compounds that cause the fruity aromas in wine. Previous work using micro fermentations showed that fermentation temperature gradients and time on skins resulted in an increase in thiol and ester compounds post fermentation and these compounds are known to cause tropical fruit aroma in wines³. This work aimed to scale up these fermentations/operations to determine if the desired aromas could still be achieved and if there is a perceivable difference in tropical fruit aromas, liking, and emotional response in the wines at the consumer level.

WINE CONSUMER TRADE-OFF BETWEEN ORGANOLEPTIC CHARACTERISTICS AND SUSTAINABLE CLAIMS. AN EXPERIMENT ON RED WINES FROM BORDEAUX REGION

In economics, the perception of wine quality is not limited to sensorial characteristics: an indication of the region of production significantly affects the perception of quality and consumers’ WTP ([1]; [2]). However, [3] or more recently [4] show that even if a wine has an organic label, the taste of wine remains the predominant criterion in consumer preferences. The contribution of our experiment is to evaluate the impact of responsible attributes (organic label, Non Added Sulfites, HVE certification) on the appreciation of several red wines on the market. More than 280 consumers participated to the present study and they perform 25 tastings divided into 5 different sessions. 20 different red wines from Bordeaux Area are tasted.

BIOPROTECTION BY ADDING NON-SACCHAROMYCES YEASTS : ADVANCED RESEARCH ON THIS PROMISING ALTERNATIVE TO SO₂

Sulphur dioxide has been used for many years for its antimicrobial, antioxidant and antioxydasic properties in winemaking but nowadays, it is a source of controversy. Indeed, consumers are more attentive to the naturalness of their foods and beverages and the legislation is changing to reduce the total SO₂ levels allowed in wines. To limit and replace the doses of sulphur dioxide applied, winemakers can now use bioprotection consisting in live yeast addition as alternative,seems to be promising. This process, lightly used in from the food industry, allows to colonize the environment and limit the development or even eliminate undesirable microorganisms without altering the sensory properties of the product.