terclim by ICS banner
IVES 9 IVES Conference Series 9 A NEW STRATEGY AND METHODOLOGY FOR THE CHARACTERIZATION OF POLYPHENOLS IN FINING PRECIPITATE

A NEW STRATEGY AND METHODOLOGY FOR THE CHARACTERIZATION OF POLYPHENOLS IN FINING PRECIPITATE

Abstract

Polyphenols are secondary metabolite widely distributed in plant kingdom such as in fruits, in grapes and in wine. During the winemaking process, polyphenols are extract from the skin and seed of the berries. Fining is an important winemaking step just before bottling which has an impact on wine stabi-lization and clarification. Most the time, fining agent are animal or vegetal protein while some of them can be synthetic polymer like PVPP or natural origin like bentonite. The aim of this study was better understand colloidals phenomenons involved in fining process and determine how polyphenols content and composition are impact by finning.

Different type and composition of fining agent were used during the investigation on each wine. Some of them were based on animal proteins, vegetal proteins, PVPP or bentonite alone while some mix with PVPP and vegetal proteins, PVPP and animal protein, and a mix with PVPP, vegetal proteins and bentonite were also used. On the wine before and after fining monomeric and total anthocyanins, monomeric, dimeric and total tannins, mDP (mean degree of polymerization), flavonol and phenolic acids content were measured. However in order to determine more precisely the content and composition of polyphenol lost during fining, a new strategy have been develop based on the re-solubilisation of the fining precipitate using some organic solvent.

For these experiments, this new method highlight some drastic differences between fining agents. Indeed, depending of the fining agent the amount and the composition of the polyphenols present in the fining precipitate change. For example, some fining agent don’t remove anthocyanins while some other precipitate mainly the p-coumarolylated anthocyanins. Moreover, important differences are also observed for condensed tannins according to the nature of the fining agent. Indeed, fining agent without PVPP were able to precipitate monomeric or dimeric condensed tannins. Moreover, some fining agent are more selective of oligomeric tannins while some has tendency to precipitate tannins with higher mDP.

This new methodology allow a more precise and clear identification of the polyphenol precipitated by fining agent and will help to better understand impact of the fining the organoleptic properties of the wine. Similarly, a better characterization of the fining precipitate will also help better understanding the colloidal structure of the wine.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Article

Authors

Gauthier Lagarde¹, Manon ferreira¹, Sandra Vanbrabant¹, Soizic Lacampagne¹, Arnaud Massot², Virginie Moine², Pierre-Louis Teissedre¹ and Michael Jourdes*,1

1. Université de Bordeaux, Bordeaux INP, Bordeaux Sciences Agro, UMR 1366 OENOLOGIE, ISVV, 33140 – Villenave d’Ornon
2. Biolaffort, 11 Rue Aristide Berges, 33270 Floirac, Gironde, France

Contact the author*

Keywords

Fining, Precipitate, Condensed tannins, Anthocyanin

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

INVESTIGATING TERROIR TYPICITY: A COMPREHENSIVE STUDY BASED ON THE AROMATIC AND SENSORIAL PROFILES OF RED WINES FROM CORBIÈRES APPELLATION

Volatile compounds play a significant role on the organoleptic properties defining wines quality. This particular role was exploited in several studies with the aim to differentiate wines from a more or less extensive production area, according to their sensory profile [1], as well as their chemical composition [2,3] (Di Paola-Naranjo et al., 2011; Kustos et al., 2020). Indeed, since aroma compounds development in grapes depends primarily on the environmental conditions of the vines and grapes (soil and climate), it is conceivable that these parameters craft the aromatic signature of the wine produced, in relation to its origin (Van Leeuwen et al., 2020). In this work, a general study on the aromatic and sensorial profile of wines produced in five sub-regions of the Corbières denomination, a renowned red grape varieties viticultural region in South France, was reported.

PRECISE AND SUSTAINABLE OENOLOGY THROUGH THE OPTIMIZED USE OF AD- JUVANTS: A BENTONITE-APPLIED MODEL OF STUDY TO EXPLOIT

As wine resilience is the result of different variables, including the wine pH and the concentration of wine components, a detailed knowledge of the relationships between the adjuvant to attain stability and the oenological medium is fundamental for process optimization and to increase wine durability till the time of consumption.

A NEW SPECIFIC LINEAGE OF OENOCOCCUS OENI IN COGNAC APPELLATION WINES

Oenococcus oeni is the main lactic acid bacteria (LAB) species which conducts the malolactic fermentation (MLF) in wine. During MLF, O. oeni converts malic acid into lactic acid, which modulates wine aroma composition leading to better balanced organoleptic properties. O. oeni is a highly specialized species only detected in environments containing alcohol such as wine, cider or kombucha. Genome analysis of more than 240 strains showed that they form at least 4 main phylogenetic lineages and several sublineages, which are associated with different beverages or types of wines.

USE OF 13C CP/MAS NMR AND EPR SPECTROSCOPIC TECHNIQUES TO CHARACTERIZE MACROMOLECULAR CHANGES IN OAK WOOD(QUERCUS PETRAEA) DURING TOASTING

For coopers, toasting process is considered a crucial step in barrel production during which oak wood (Q. petraea) develops several aromatic nuances released to the wine during its maturation. Toasting consists of applying different degrees of heat to a barrel for a specific period. As the temperature increases, thermal degradation of oak wood structure produces a huge range of chemical compounds. Many studies have identified the main key aroma volatile compounds (whisky-lactone, furfural, eugenol, guaiacol, vanillin). However, detailed information on how the chemical structure of oak wood degrades with increasing toasting level is still lacking.

AROMA AND SENSORY CHARACTERIZATION OF XINOMAVRO RED WINES FROM DIFFERENT GREEK PROTECTED DESIGNATIONS OF ORIGIN, EFFECT OF TERROIR CHARACTERISTICS

The quality of wines has often been associated with their geographical area of production. The aim of this work was to characterize Protected Designation of Origin (PDO) Xinomavro red wines from different geographical areas of Amyndeon and Naoussa in Northern Greece, elaborated with variables that contribute to their differentiation, such as soil characteristics, altitude, monthly average temperature and rainfall.
Xinomavro fruit parcels from different vineyards within the two PDO zones (5 PDO Naoussa and 6 PDO Amyndeon) were vinified following a standard winemaking process. A total of 25 aroma compounds were quantified using gas chromatography-mass spectrometry (GC-MS) with simultaneous full scan and selected ion monitoring for data recording, and odor activity values (OAVs) were determined.