Study of yeast biocatalytic activity on grape aroma compounds

Abstract

Many volatile compounds of different chemical/biochemical origin contribute to wine aroma. Certain key ‘varietal’ aroma compounds such as methoxypyrazines are formed in the grape and appear to be only scarcely influenced by fermentation. Conversely, other grape-derived compounds undergo important transformation during fermentation, so that grape varietal volatile pattern is substantially different than the one observed in the corresponding wine. While this phenomenon is generally regarded from the point of view of the cleavage of glycosidic or amino acidic precursors, recent studies highlighted the existence of other bio-catalytic processes taking place during fermentation, which could be relevant to wine aroma generation. Accordingly, in addition to enzymatic activities such as glycosidase, b-lyase, and/or acetyl-transferase, other enzymes could play a role in the expression of wine varietal aroma typicality, although these have been poorly characterized so far. Certain key volatile such as norisoprenoids (fruity attributes), lactones, (dry fruit attributes), cyclic terpenoids (minty and balsamic attributes), sesquiterpenes and benzenoids (balsamic and spicy attributes) could be associated with such processes. Some of them could also arise from the combination of yeast enzymatic and acidic rearrangements taking place at wine pH. 

The aim of this work was to investigate the biotransformation of potentially relevant grape metabolites by Saccharomyces cerevisiae. Cyclic, oxydrylated, or ketonic terpenes, sesquiterpenes, aliphatic lactones and aldehydes, hydroxyl acids and benzenoids were all investigated, as well as precursors extracts from different grapes. Biotransformations were screened by placing target compounds under incubation (at 37 °C) with yeast resting cells for 72 hours under variable conditions. After incubation, the products of biotransformation were analyzed by SPME-GC-MS and their aroma evaluated by GC-O. 

The results highlighted the occurrence of several complex transformations involving, among others, reduction of allylic carbonyl and carbon-carbon double bond, stereospecific reduction of terpenic ketones, acetylation. These reactions occurring to grape metabolites produced odoriferous molecules considered to participate to the characteristic aroma of some wines. The methodology employed in our study turned out as an effective approach to study the process of aroma generation from neutral grape into wine. As first application, this study has allowed to elucidate some aspects concerning the balsamic notes appearing in wines made with Corvina grapes.

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Davide Slaghenaufi, Eleonora Troiano, Giovanna E. Felis, Maurizio Ugliano

University of Verona, Department of Biotechnology, Villa Ottolini-Lebrecht via della Pieve, 70 37029 San Floriano (VR) – Italy

Contact the author

Keywords

aroma, yeast, terpenes, biocatalysis

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Volatilome in grapevine leaves is defined by the variety and modulated by mycorrhizal symbiosis

Volatile organic compounds (VOCs) constitute a diverse group of secondary metabolites key for the communication of plants with other organisms and for their adaptation to environmental and biotic stresses. The emission of these compounds through leaves is also affected by the interaction of plants with symbiotic microorganisms, arbuscular mycorrhizal fungi (AMF) among them [1]. Our objective was to know the concentration and profile of VOCs emitted by the leaves of two grapevine varieties (Tempranillo, T, and Cabernet Sauvignon, CS, grafted onto R110 rootstocks), inoculated or not with a consortium of five AMF (Rhizophagus irregularis, Funneliformis mosseae, Septoglomus deserticola, Claroideoglomus claroideum and C. etunicatum).

EFFECT OF DIFFERENT VITICULTURAL AND ENOLOGICAL PRACTICES ON THE PHENOLIC COMPOSITION OF RED WINES

Global climate change is exerting a notable influence on viticulture sector and grape composition. The increase in temperature and the changes in rainfall pattern are causing a gap between phenolic and technological grape maturities [1]. As a result, the composition of grapes at harvest time and, consequently, that of wines are being affected, especially with regards to phenolic composition. Hence, wine quality is decreasing due to changes in the organoleptic properties, such as color and astringency, making necessary to implement new adaptive technologies in wineries to modulate these properties in order to improve wine quality.

Harvest dates, climate, and viticultural region zoning in Greece

Climate is clearly one of the most important factors in the success of all agricultural systems, influencing whether a crop is suitable to a given region, largely controlling crop production and quality, and ultimately driving economic sustainability. Today many assessments of a region’s climate comes from a combination of station and spatial climate data analyses that facilitate the evaluation of the general suitability for viticulture and potential wine styles, allows for comparisons between wine regions, and offers growers a measure of assessing appropriate cultivars and sites.

Study of the volatile aroma profile of five Italian grape varieties submitted to controlled postharvest withering

Wines made with grapes submitted to postharvest dehydration are often referred to as “passito” or “straw wines.” This distinct style of winemaking consists of a process of water loss that allows the berries to undergo a mild water stress and senescence process [1].

Relation between the environmental factors of the terroir system and flavan-3-ol composition of grape berry seeds and skin at pre-veraison stage and harvest Influence of dedicate viticultural management

Quantity and quality of flavonoïds in grape berries are important parts of their global quality. Several studies had shown that tannins are responsible for some major flavour properties of red wines such as colour, bitterness and astringency. Nevertheless, their synthesis and properties are still misunderstood. Some studies had suggested that the tannic pool was set before veraison. Thus, the comprehension of the relations between environment and setting of this tannic pool, up to the harvest, is not sufficient.