Multi-omics methods to unravel microbial diversity in fermentation of Riesling wines

Microbial pathogens of plant have evolved to sense, interpret, and use light to direct their development. One aspect of this evolved relationship is photolyase-mediated repair of UV-induced damage to pathogen DNA. Application of germicidal UV (UV-C) at night circumvents the blue light-driven repair of pathogen DNA and allows non-phytotoxic doses of UV-C to suppress a variety of pathogenic microbes and even certain arthropod pests without damage to vines or fruit. Lamps arrays have been designed specifically for the canopy architecture of grapevines and have been deployed on both tractor-drawn and robotic carriages for partial to near-complete suppression of powdery mildew (Erysiphe necator), sour rot (fungal, bacterial, and arthropod complex), and downy mildew (Plasmopara viticola).

Fast, accurate, and non-destructive analytical techniques based on hyperspectral imaging (hsi) represent effective tools for food quality evaluation. A visible change in the appearance of a fresh product often negatively impacts the perceived quality from a consumer’s point of view.

The research carried out by the URVV of the INRA center in Angers aims to develop a methodology for the integrated characterization of the natural factors of viticultural terroirs, representative of the operating conditions of the vine and the sensory differences of the wines. In this context, the concept of Basic Terroir Unit (UTB) has been developed. The UTB represents a viticultural surface of variable geographical extension, defined as the association in a given place of a geological, pedological and landscape component, Morlat (1989), Riou et al. (1995).

During alcoholic fermentation, nitrogen is an essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (yan) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species which may lead to economic losses. However, correcting this nitrogen deficiency is sometimes not enough to restore proper fermentation performance. This suggests the existence of other nutritional shortages.

Many works have been carried out to identify the key aroma volatile compounds of oak wood (e.g., whisky-lactone, furfural, maltol, eugenol, guaiacol, vanillin) using conventional gas chromatography coupled with olfactometry and mass spectrometry (GC-O-MS). Inspired by recent untargeted approaches in the field of food “omics”, this work aims to extend our knowledge on the impact of cooperage process on the volatile composition of oak wood using two-dimensional comprehensive gas chromatography coupled with time of flight mass spectrometry (GCxGC-ToFMS).