OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Chemical and Biochemical reactions, including grape and wines microorganisms impact 9 Multi-omics methods to unravel microbial diversity in fermentation of Riesling wines

Multi-omics methods to unravel microbial diversity in fermentation of Riesling wines

Abstract

Wine aroma is shaped by the wine’s chemical compositions, in which both grape constituents and microbes play crucial roles. Although wine quality is influenced by the microbial communities, less is known about their population interactions. Previous studies linking the effect of native microbial communities to sensory relevant aroma compounds with their interactive properties have been vastly unsuccessful to date. Partially because studies relied on relatively few isolated strains or chemical compounds, which may be not sufficient to fully understand this complex picture.

Native microbial communities from different Riesling vineyards were studied over multiple experiments during vinification as well as over a two-year to reveal their effects on chemical and sensory composition of spontaneously fermented Riesling wines.

We demonstrate that by combining modern untargeted high-throughput omics technologies and statistical approaches, it is possible to look into samples in situ in the actual natural environment. Our results indicate that both vineyard and winery microbial communities are found to play significant roles in wine. Microbial communities within the fermenting were strongly influenced by vineyard of origin.

These population dynamics are consequently translated into diverse sensory properties through sensory relevant chemical interactions. We found that both sensory and chemical compositions were heavily influenced by the microbial community composition during the vinification as well as the vineyard and the year. Such methodologies allow to find novel microbial and chemical patterns which could be further tested with targeted studies. In addition to deconstructing the microbial community composition in complex natural environment, we leverage on shotgun metagenomic data to undertake the functional analysis of the microbial community during wine fermentation. In the future, multiomics approaches will be essential for fully discovering the complexity of biological networks, where microbes, host and chemical compounds interact with human sensory perceptions. These developed approaches benefit any industry that works with complex biological interactions.

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Kimmo SIREN Sarah Siu Tze Mak, M. Thomas P. Gilbert, Ulrich Fischer

Section for Evolutionary Genomics, Natural History Museum of Denmark, University of Copenhagen, Co-penhagen, Denmark
Institute for Viticulture & Oenology, DLR Rheinpfalz, Neustadt/Wstr.,Germany

Contact the author

Keywords

Metagenomics, Metabarcoding, Chemical interactions, Machine learning 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Effect of “Terroir” on quanti-qualitative paramethers of “vino nobile di Montepulciano”

In this last ten years period, there has been many integrated and interdisciplinary studies to determine the aptitude of different zones to viticulture (Lulli et al., 1989 ; Costantini, 1992 ; Fregoni et al., 1992). The researches needed some différent knowledges about environment characteristics (soil, climate), ecology, vineyard management, vine genetic, winemaking and sensory analysis. The interaction of all these knowledge produced the assessment about the environmental vocation (Scienza et al., 1992). By means of this metodology, the “viticultural vocation” joined the word “zoning”, that is the territory parting for its ecopedological and geographical characteristics in relation to adaptative answer of winegrape (Morlat, 1989).

Effects of water deficit on secondary metabolites in grapes and wines

In this video recording of the IVES science meeting 2021, Simone D. Castellarin (University of British Columbia, Wine Research Center, Wine Research Centre, Vancouver, Canada) speaks about the effects of water deficit on secondary metabolites in grapes and wines. This presentation is based on an original article accessible for free on OENO One.

Advancing grapevine science through genomic research

The seminar will examine the complexities and prospects of genomic research on Vitis species, characterize by exceptionally high heterozygosity and common interspecific gene flow. The seminar will showcase case studies highlighting the critical role of diploid genome references in grape research, specifically in areas such as aroma development, disease resistance, and domestication traits. It will also address the emerging focus on pangenomes within the Vitis genus, particularly in the context of genetic studies on naturally interbreeding populations.

Dormancy conundrum: thermal requirements plasticity to reach budburst may be explained by annual environmental dynamics

Deciphering grapevine dormancy is crucial in the current context of climatic challenges: advancing budburst phenology and increased late frost probabilities, observed in the last decades and expected to further increase, require deeper understanding. Beyond higher mean temperatures, abiotic stresses such as water deficit have also been emphasized as actors. In this framework, we aimed at exploring new methodologies for tracking dormancy cycle and testing the interplay on its regulation of temperature dynamics and drought.
In a first experiment, twenty-one Vitis vinifera varieties were monitored during ecodormancy and budburst over three years.

Nitrogen metabolism in Kluyveromyces marxianus and Saccharomyces cerevisiae: towards a better understanding of fermentation aroma production

During wine alcoholic fermentation, yeasts produce volatile aroma compounds from sugar and nitrogen metabolism. Some of the metabolic pathways leading to these compounds have been known for more than a century.