Multi-omics methods to unravel microbial diversity in fermentation of Riesling wines

The frequency of water deficits is increasing in many grape-growing regions due to climate change.

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001).

In mediterranean agriculture, sustainability and productivity are seriously threatened by climate change and water scarcity. This situation is exacerbated by poor management practices such as excessive use of agrochemicals, overgrazing, and monoculture. The Douro demarcated region (ddr) is an emblematic region, classified world heritage site by UNESCO in 2001. Viticulture is the main agricultural activity in DDR, widely known to produce port wine.

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

Vineyard nutrient management is crucial for reaching production-specific quality standards, yet timely evaluation of nutrient status remains challenging. The existing sampling protocol of collecting vine tissue (leaves and/or petioles) at bloom or veraison is time-consuming. Additionally, this sampling practice is too late for in-season fertilizer applications (e.g. N is applied well before bloom). Therefore alternative early-season protocols are necessary to predict the vine nutrient demand for the upcoming season. The main goals of this project are to 1) optimize existing tissue sampling protocols; 2) determine the amount of nutrients removed at the end of the growing season.