OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 OENO IVAS 9 OENO IVAS 2019 9 Chemical and Biochemical reactions, including grape and wines microorganisms impact 9 Multi-omics methods to unravel microbial diversity in fermentation of Riesling wines

Multi-omics methods to unravel microbial diversity in fermentation of Riesling wines

Abstract

Wine aroma is shaped by the wine’s chemical compositions, in which both grape constituents and microbes play crucial roles. Although wine quality is influenced by the microbial communities, less is known about their population interactions. Previous studies linking the effect of native microbial communities to sensory relevant aroma compounds with their interactive properties have been vastly unsuccessful to date. Partially because studies relied on relatively few isolated strains or chemical compounds, which may be not sufficient to fully understand this complex picture.

Native microbial communities from different Riesling vineyards were studied over multiple experiments during vinification as well as over a two-year to reveal their effects on chemical and sensory composition of spontaneously fermented Riesling wines.

We demonstrate that by combining modern untargeted high-throughput omics technologies and statistical approaches, it is possible to look into samples in situ in the actual natural environment. Our results indicate that both vineyard and winery microbial communities are found to play significant roles in wine. Microbial communities within the fermenting were strongly influenced by vineyard of origin.

These population dynamics are consequently translated into diverse sensory properties through sensory relevant chemical interactions. We found that both sensory and chemical compositions were heavily influenced by the microbial community composition during the vinification as well as the vineyard and the year. Such methodologies allow to find novel microbial and chemical patterns which could be further tested with targeted studies. In addition to deconstructing the microbial community composition in complex natural environment, we leverage on shotgun metagenomic data to undertake the functional analysis of the microbial community during wine fermentation. In the future, multiomics approaches will be essential for fully discovering the complexity of biological networks, where microbes, host and chemical compounds interact with human sensory perceptions. These developed approaches benefit any industry that works with complex biological interactions.

DOI:

Publication date: June 19, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Kimmo SIREN Sarah Siu Tze Mak, M. Thomas P. Gilbert, Ulrich Fischer

Section for Evolutionary Genomics, Natural History Museum of Denmark, University of Copenhagen, Co-penhagen, Denmark
Institute for Viticulture & Oenology, DLR Rheinpfalz, Neustadt/Wstr.,Germany

Contact the author

Keywords

Metagenomics, Metabarcoding, Chemical interactions, Machine learning 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Organic and biodynamic sustainable productions and effect on eligibility and peculiarity of a typical wine

The wine industry is currently shifting toward more sustainable production systems. There are many reasons for this as the interest of people over climate change and, consequently the wine consumer’s choice toward organic and biodynamic, reduced carbon-footprint, vegan and other environmentally friendly wines. While the viticultural effects of biodynamic and organic practices on wine grapes have been investigated, there is a lack in literature on the general effect on the final quality of wine

Physiological and growth reaction of Shiraz/101-14 Mgt to row orientation and soil water status

Advanced knowledge on grapevine row orientation is required to improve establishment, management and outcomes of vineyards on terroirs with different environmental conditions (climate, soil, topography) and in view of a future change to more extreme climatic conditions. The purpose of this study was to determine the combined effect of row orientation, plant water status and ripeness level on the physiological and viticultural reaction of Shiraz/101-14 Mgt.

Understanding the complexity of grapevine winter physiology in the face of changing climate

The vast majority of our understanding of grapevine physiology is focused on the processes that occur during the growing season. Though not obvious, winter physiological changes are dynamic and complex, and have great influence on the survival and phenology of grapevines. In cool and cold climates, winter temperatures are a constant threat to vine survival. Additionally, as climate changes, grapevine production is moving toward more traditionally cool and cold climates, either latitudinal or altitudinal in location. Our research focuses on understanding how grapevines navigate winter physiological changes and how temperature impacts aspects of cold hardiness and dormancy. Through these studies, we have gained keen insight into the connections between winter temperature, maximum cold haridness, and budbreak phenology, that can be used to develop prediction models for viticulture in a changing climate.

Extreme vintages affect grape varieties differently: a case study from a cool climate wine region

Eger wine region is located on the northern border of grapevine cultivation zone. In the cool climate, terroir selection is one of the foundations of quality wine making. However, climate change will have a significant impact on these high value-added vineyards. This study presents a case study from 2021 and 2022 with the investigation of three grape varieties (Kadarka, Syrah, Furmint). The experiment was conducted in a steep-sloped vineyard (Nagy-Eged hill) with a southern exposure.

FACTORS AFFECTING QUERCETIN SOLUBILITY IN SANGIOVESE RED WINE: FIRST RESULTS

Quercetin (Q) is present in grape in form of glycosides and as aglycone. These compounds are extracted from grape skins during winemaking. In wines, following the hydrolysis reactions, the amount of quercetin aglycon can exceed its solubility value. Unfortunately, a threshold solubility concentration for quercetin in wine is not easy to determine because it depends on wine matrix (Gambuti et al., 2020).