terclim by ICS banner
IVES 9 IVES Conference Series 9 FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

Abstract

Temperature control is crucial in wine production, starting from grape harvest to the bottled wine storage. Climate change and global warming affect the timing of grape ripening, and harvesting is often done during hot summer days, influencing berry integrity, secondary metabolites potential, enzyme and oxidation phenomena, and even fermentation kinetics. To curb this phenomenon, pre-fermentative cold storage can help preserve the grapes and possibly increase the concentration of key secondary metabolites.

In this study, the effect of grape pre-fermentative cold storage was assessed on the ‘Moscato bianco’ white grape cultivar, known for its varietal terpenes (65% of free terpenes represented by linalool and its derivatives) and widely used in Piedmont (Italy) to produce Asti DOCG wines. The study involved two experiments: a 12 h short-term storage under fresh (15 °C) and sunny outdoor (peak of 43 °C) conditions, and a medium-term storage under five different temperatures (5, 7, 12, 17, 19 °C) and durations (12, 24, 54, 84, 96 h), according to a Central Composite Design then evaluated using response-surface methodology (RSM). Berry skin break force mechanical property and juice physiochemical parameters were analyzed, as well as juice free terpene compounds using GC-MS.

In the short-term trial, after 4 and 8 h of storage the cooled sample showed a higher concentration of linalool, but at the end of the storage (12 h, when external temperature dropped to 25-20 °C after sunset), an opposite situation was found, possibly indicating a higher terpene solubilization in their thermotolerance defense role.

The medium-storage experiment indicated that the sum of the 13 detected terpenes in grape juice significantly decreased progressively after 75 h of storage, particularly in samples stored at the highest temperature tested (19 °C). However, the RSM model indicates that storage times shorter than 50 h contributed to higher terpenes, as well as the increase in storage temperature. The berry skin break force was not affected significantly by the treatments.

In conclusion, grape cold storage may offer several advantages in winemaking, but further studies are needed on this variety for assessing the best storage temperature and length conditions, as well as for the comparison between free and glycosidically-bound terpenes in juice and in the resulting wine. Acknowledgments. We thank Marco Rossetto and DENSO Thermal Systems (Poirino, Italy) for their support to this study.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Simone Giacosa¹, Stefania Savoi¹, Annachiara Lenti¹, Susana Río Segade¹, Maria Alessandra Paissoni¹, Andrea Bellincontro², Fabio Mencarelli³, Luca Rolle¹

1. University of Torino, Department of Agricultural, Forest and Food Sciences. Corso Enotria 2/C, 12051 Alba (CN), Italy
2. University of Tuscia, Department for Innovation in Biological, Agro-food and Forest systems. Via San Camillo de Lellis snc, Viterbo, Italy
3. University of Pisa, Department of Agriculture, Food and Environment. Via del Borghetto 80, Pisa, Italy

Contact the author*

Keywords

grape cold storage, aroma, terpenes, Muscat varieties

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Metabolomics for grape and wine research: exploring the contributions of amino acids to wine flavour

A critical aspect of wine quality is the overall expression of wine flavour, which is formed by the interplay of volatile aroma compounds, their precursors, and taste and matrix components.
Grapes directly contribute to wine only a small number of potent aroma compounds, and the unique
sensory attributes and perceived quality of a wine result from combining 100s of metabolites of grapes, yeast and bacteria, and oak wood.

EVIDENCE OF THE INTERACTION OF ULTRASOUND AND ASPERGILLOPEPSINS I ON UNSTABLE GRAPE PROTEINS

Most of the effects of ultrasound (US) result from the collapse of bubbles due to cavitation. The shockwave produced is associated with shear forces, along with high localised temperatures and pressures. However, the high-speed stream, radical species formation, and heat generated during sonication may also affect the stability of some enzymes and proteins, depending on their chemical structure. Recently, Ce-lotti et al. (2021) reported the effects of US on protein stability in wines. To investigate this further, the effect of temperature (40°C and 70°C; 60s), sonication (20 kHz and 100 % amplitude, for 20s and 60s, leading to the same temperatures as above, respectively), in combination with Aspergillopepsins I (AP-I) supplementation (100 μg/L), was studied on unstable protein concentration (TLPs and chitinases) using HPLC with an UV–Vis detector in a TLPs-supplemented model system and in an unstable white wine.

ESTIMATING THE INITIAL OXYGEN RELEASE (IOR) OF CORK CLOSURES

Many factors influence aging of bottled wine, oxygen transfer through the closure is included. The maximum uptake of wine before oxidation begins varies from 60 mg.L-¹ to 180 mg.L-1 for white and red wines respectively [1].
The process of bottling may lead to considerable amounts of oxygen. The actual contribution of the transfer through the closure system becomes relevant at the bottle storage, but the amounts are small compared to prepacking operations [2] and to the total oxygen attained during filling.

IMPACT OF HARVEST DATE ON THE FINE MOLECULAR COMPOSITION OF MUST AND BORDEAUX RED WINE (VAR. MERLOT, CABERNET SAUVIGNON). FOCUS ON ACIDITY AND SENSORY IMPACT AFTER FIVE YEARS OF AGING

Climate change has brought several impacts that are becoming increasingly intense during the last few years and put at risk the quality of the berries or even the plant’s sustainability. Such extreme climatic events impact the composition of the wine while modulating its quality and the consumer preferences (Tempère et al., 2019). The three most important changes that take place in the must are: 1) decrease acidity, 2) increase of the concentration of sugar, hence increase of alcohol in the wine, and 3) modification
of the sensory balance and the development for example of cooked fruit aromas.

INSIGHTS ON THE ROLE OF GENES ON AROMA FORMATION OF WINES

Yeast secondary metabolism is a complex network of biochemical pathways and the genetic profile of the yeast carrying out the alcoholic fermentation is obviously important in the formation of the metabolites conferring specific odors to wine. The aim of the present research was to investigate the relative expression of genes involved in flavor compound production in eight different Saccharomyces cerevisiae strains.
Two commercial yeast strains Sc1 (S.cerevisiae x S.bayanus) and Sc2 (S.cerevisiae) and six indigenous S. cerevisiae strains (Sc3, Sc4, Sc5, Sc6, Sc7, Sc8) isolated during spontaneous fermentations were inoculated in Assyrtiko and Vidiano grape must.