terclim by ICS banner
IVES 9 IVES Conference Series 9 FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

Abstract

Temperature control is crucial in wine production, starting from grape harvest to the bottled wine storage. Climate change and global warming affect the timing of grape ripening, and harvesting is often done during hot summer days, influencing berry integrity, secondary metabolites potential, enzyme and oxidation phenomena, and even fermentation kinetics. To curb this phenomenon, pre-fermentative cold storage can help preserve the grapes and possibly increase the concentration of key secondary metabolites.

In this study, the effect of grape pre-fermentative cold storage was assessed on the ‘Moscato bianco’ white grape cultivar, known for its varietal terpenes (65% of free terpenes represented by linalool and its derivatives) and widely used in Piedmont (Italy) to produce Asti DOCG wines. The study involved two experiments: a 12 h short-term storage under fresh (15 °C) and sunny outdoor (peak of 43 °C) conditions, and a medium-term storage under five different temperatures (5, 7, 12, 17, 19 °C) and durations (12, 24, 54, 84, 96 h), according to a Central Composite Design then evaluated using response-surface methodology (RSM). Berry skin break force mechanical property and juice physiochemical parameters were analyzed, as well as juice free terpene compounds using GC-MS.

In the short-term trial, after 4 and 8 h of storage the cooled sample showed a higher concentration of linalool, but at the end of the storage (12 h, when external temperature dropped to 25-20 °C after sunset), an opposite situation was found, possibly indicating a higher terpene solubilization in their thermotolerance defense role.

The medium-storage experiment indicated that the sum of the 13 detected terpenes in grape juice significantly decreased progressively after 75 h of storage, particularly in samples stored at the highest temperature tested (19 °C). However, the RSM model indicates that storage times shorter than 50 h contributed to higher terpenes, as well as the increase in storage temperature. The berry skin break force was not affected significantly by the treatments.

In conclusion, grape cold storage may offer several advantages in winemaking, but further studies are needed on this variety for assessing the best storage temperature and length conditions, as well as for the comparison between free and glycosidically-bound terpenes in juice and in the resulting wine. Acknowledgments. We thank Marco Rossetto and DENSO Thermal Systems (Poirino, Italy) for their support to this study.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Simone Giacosa¹, Stefania Savoi¹, Annachiara Lenti¹, Susana Río Segade¹, Maria Alessandra Paissoni¹, Andrea Bellincontro², Fabio Mencarelli³, Luca Rolle¹

1. University of Torino, Department of Agricultural, Forest and Food Sciences. Corso Enotria 2/C, 12051 Alba (CN), Italy
2. University of Tuscia, Department for Innovation in Biological, Agro-food and Forest systems. Via San Camillo de Lellis snc, Viterbo, Italy
3. University of Pisa, Department of Agriculture, Food and Environment. Via del Borghetto 80, Pisa, Italy

Contact the author*

Keywords

grape cold storage, aroma, terpenes, Muscat varieties

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

NEW TOOL FOR SIMULTANEOUS MEASUREMENT OF OXYGEN CONSUMPTION AND COLOUR MODIFICATIONS IN WINES

Measuring the effect of oxygen consumption on the colour of wines as the level of dissolved oxygen decreases over time is very useful to know how much oxygen a wine is able to consume without significantly altering its colour. The changes produced in wine after being exposed to high oxygen concen-trations have been studied by different authors, but in all cases the wine has been analysed once the oxygen consumption process has been completed. This work presents the results obtained with the use of an equipment designed and made to measure simultaneously the level of dissolved oxygen and the spectrum of the wine, during the oxygen consumption process from saturation levels with air to very low levels, which indicate the total consumption of the dosed oxygen.

THE IMPACT OF NON-SACCHAROMYCES YEASTS ON THE WHITE WINE QUALITY

Selected strains of non-Saccharomyces yeasts showed a positive effect on sensory characteristics and aromatic complexity of wine. A sequential microbial culture of non-Saccharomyces and S. cerevisiae species is usually inoculated due to poorer fermentability of non-Saccharomyces species. The aim of the study was to investigate the role of non-Saccharomyces yeasts in the production of white wines. We evaluated how individual combinations of sequential inoculations of non-Saccharomyces and S. cerevisiae species affect the aromatic compounds (volatile thiols and esters) and sensory characteristics of the wines.

INOCULATION OF THE SELECTED METSCHNIKOWIA PULCHERRIMA MP1 AS A BIOPROTECTIVE ALTERNATIVE TO SULFITES TO PREVENT BROWNING OF WHITE GRAPE MUST

Enzymatic browning (BE) of must is caused by polyphenol oxidases (PPOs), tyrosinase and laccase. Both PPOs can oxidize diphenols such as hydroxycinnamic acids (HA) to quinones, which can later polymerize to form melanins [1], which are responsible of BE in white wines and of oxidasic haze in red wines. SO₂ is the main tool used to protect must from BE thanks to its capacity to inhibit PPOs [2]. However, the current trend in winemaking is to reduce and even eliminate this unfriendly additive. Among the different possible alternatives for protecting must against BE, the inoculation with a selected Metschnikowia pulcherrima MP1 is without any doubt one of the most promising ones.

THE EFFECT OF BENTONITE FINING ON THE VOLATILE AND NON-VOLATILE PROFILE OF ITALIAN WHITE WINES

Marselan wines have an unusual high proportion of seed derived tannins from grapes having high proportions of skins, which are rich in tannins. But the causes behind this characteristic have not yet been identified. In vintage 2023 wines were made at experimental scale (9 kg by experimental unit) from Arinarnoa, Marselan and Tannat Vitis vinifera grape cultivars by traditional maceration, and by techniques aimed to increase the wine content in skin derived tannin: addition of extraction enzymes, addition at vatting of grape-skin enological tannins, or by extended maceration, known to increase the seed derived tannin contents of wines. Macerations were of 7 days, except in the extended macerations that were of 15 days.

DISCRIMINATION OF BOTRYTIS CINEREA INFECTED GRAPES USING UNTARGE-TED METABOLOMIC ANALYSIS WITH DIRECT ELECTROSPRAY IONISATION MASS SPECTROMETRY

Infection of grapes (Vitis vinifera) by Botrytis cinerea (grey mould) is a frequent occurrence in vineyards and during prolonged wet and humid conditions can lead to significant detrimental impact on yield and overall quality. Growth of B. cinerea causes oxidisation of phenolic compounds resulting in a loss of colour and formation of a suite of off-flavours and odours in wine made from excessively infected fruit. Apart from wine grapes, developing post-harvest B. cinerea infection in high-value horticultural products during storage, shipment and marketing may cause significant loss in fresh fruits, vegetables and other crops. A rapid and sensitive assessment method to detect, screen and quantify fungal infection would greatly assist viticultural growers and winemakers in determining fruit quality.