Fully automated non-targeted GC-MS data analysis

The objective of this study is to review the scientific evidences and to advance into the knowledge of the molecular mechanisms of astringency. Astringency has been described as the drying, roughing and puckering sensation perceived when some food and beverages are tasted (1). The main, but possibly not the only, mechanism for the astringency is the precipitation of salivary proteins (2,3). Between phenolic compounds found in red wines, flavan-3-ols are the group usually related to the development of this sensation. Other compounds, phenolic or not, like anthocyanins, polysaccharides and mannoproteins could act modifying or modulating astringency perception by hindering the interaction between flavanols and salivary proteins either because of their interaction with the flavanols or because of their interaction with the salivary proteins.

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.

Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease worldwide. Most commercially important cultivars of the European grapevine are highly susceptible and therefore require the recurrent application of synthetic fungicides to control the disease, copper being the most frequently used. However, with European Union goals to lower their usage, there is a need to develop innovative and sustainable strategies. In this respect, seaweeds have proven to have great potential as phytosanitary agents, in addition to promoting plant growth and stress-tolerance.

Soil management through cover crops can influence the cycle of nutrients, promote water infiltration, decrease erosion, and enhance the soil microbiota biodiversity, improving the grapevine performance. However, the area under the vines tends to be left bare by applying herbicides or tillage to avoid competition with the crop in semi-arid climates. Use of covers under-vine might be an alternative to these practices aiming at grapevine quality and soil health improvement. The aim of this research was to study the implications of soil management under the vines (cultivation and cover crops) on growth, yield, berry composition and soil microbial communities. A cover crop composed by a mixture of legumes was sown and compared with a control (cultivation), which includes frequent tillage to keep the soil bare, in three areas characterized by different edaphoclimatic conditions in the region of Navarra.

Among all pathologies that afflict grapevine, Downy Mildew (DM) is the most important. Generally controlled using Copper (Cu), recently European Commission confirmed its usage but limiting the maximum amount to 28 Kg per hectare in 7 years (Reg. EU 2018/1981).