terclim by ICS banner
IVES 9 IVES Conference Series 9 NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

NEW METHOD FOR THE QUANTIFICATION OF CONDENSED TANNINS AND OTHER WINE PHENOLIC COMPOUNDS USING THE AUTOMATED BIOSYSTEMS SPICA ANALIZER

Abstract

Wine phenolic compounds are important secondary metabolites in enology due to their antioxidant and nutraceutical properties, and their role in the development of color, taste, and protection of wine from oxidation and spoilage. Tannins are valuable phenolic compounds that contribute significantly to these wine properties, especially in mouthfeel characteristics; however, tannin determination remains a significant challenge, with manual and time-consuming methods or complex methodologies. The purpose of this study is to propose a novel method for quantifying condensed tannins in finished wine products. This method aims to provide an accurate approximation of condensed tannin levels, similar to the widely used precipitation assay that involves the polysaccharide polymer methyl cellulose. The new tannin approximation is based on the strong correlation observed between Total Polyphenol Index (TPI) and methyl cellulose precipitable (MCP) tannin assay as both are determined at 280 nm, and using the epicatechin calibration is possible to obtain a value that is equivalent to condensed tannins determined by MCP tannin assay. Thus far, the results have shown a strong correlation between this new method and MCP tannin assay, with an r2 value of 0.83 and a sample size of 60. The present study has included wines from diverse geographical locations and varying ages. In addition to quantifying condensed tannins, the study also aimed to explore potential correlations that may explain differences found in the wines by analysing other polyphenolic parameters. These parameters included catechins determined via the DMACA reaction, anthocyanins based on their structural transformation arising from a change in pH, total polyphenols measured using the Folin-Ciocalteu reaction, TPI, and the chromatic features of wines at 420, 520 and 620 nm. Moreover, the study incorporated analyses of pH, total acidity, and ethanol content to obtain a comprehensive understanding of the wines’ chemical composition. Noteworthy, the study is expanding the sampling to consider more matrices within the winemaking process.

All of the aforementioned parameters, including the condensed tannin, were determined automatically using the BioSystems SPICA® analyzer. The technical simplicity of automated methods for phenolic evaluation, will lead to enhanced efficiency, robustness, and accuracy. Furthermore, these automated methods may facilitate greater field applications, leading to increased profitability and an opportunity to improve wine quality.

 

1. Vignault A, González-Centeno MR, Pascual O, Gombau J, Jourdes M, Moine V, et al. Chemical characterization, antioxidant properties and oxygen consumption rate of 36 commercial oenological tannins in a model wine solution. Food Chem. 2018 Dec 1;268:210–9.
2. Gutiérrez-Escobar R, Aliaño-González MJ, Cantos-Villar E. Wine polyphenol content and its influence on wine quality and properties: A review. Vol. 26, Molecules. MDPI AG; 2021.
3. Ribereau-Gayon P, Glories Y. Handbook of Enology:The Chemistry of Wine Stabilization and Treatments. 2006.
4. Habertson J, Spayd S. Measuring Phenolics in the winery. Am J Enol Vitic. 2006;57(3).

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Marilyn M. García-Tenesaca¹, Andreu Tobeña Montanuy²

1. Biosystems S.A , Costa Brava 30, Barcelona, Spain

Contact the author*

Keywords

Tannins, Polyphenolic profile, Automated methods, Wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

AROMA ASSESSMENT OF COMMERCIAL SFORZATO DI VALTELLINA WINES BYINSTRUMENTAL AND SENSORY METHODOLOGIES

Sforzato di Valtellina DOCG is a special dry red wine produced from partially dehydrated Nebbiolo wine-grapes growing in the Rhaetian Alps valley of Valtellina (Lombardy, Italy). Valtellina terraced vineyards are located at an altitude of 350–800 m according to ‘heroic’ viticulture on steep slopes. The harvested grape bunches are naturally dehydrated indoors, where a slow and continuous withering occurs (about 20% w/w of weight loss), until at least 1st December when the grapes reach the desired sugar content and can be processed following a normal winemaking with maceration.

REMEDIATION OF SMOKE TAINTED WINE USING MOLECULARLY IMPRINTED POLYMERS

In recent years, vineyards in Australia, the US, Canada, Chile, South Africa and Europe have been exposed to smoke from wildfires. Wines made from smoke-affected grapes often exhibit unpleasant smoky, ashy characters, attributed to the presence of smoke-derived volatile compounds, including volatile phenols (which occur in free and glycosylated forms). Various strategies for remediation of smoke tainted wine have been evaluated. The most effective strategies involve the removal of smoke taint compounds via the addition of adsorbent materials such as activated carbon, which can either be added directly or used in combination with nanofiltration. However, these treatments often simultaneously remove wine constituents responsible for desirable aroma, flavour and colour attributes.

PHOTOCHEMICAL DEGRADATION OF TRYPTOPHAN IN MODEL WINE: IMPACT OF HEAVY METALS AND OXYGEN ON 2-AMINOACETOPHENONE FORMATION

The wine industry worldwide faces more and more challenges due to climate change, such as increased dryness in some areas, water stress, sunburn and early harvesting during hot summer temperatures¹. One of the resulting problems for the wine quality might be a higher prevalence of the untypical aging off-flavor (ATA)². A substance, which Rapp and Versini made responsible for ATA, is the 2-aminoace-tophenone (2-AAP)³. 2-AAP in wine causes a naphthalene, wet towels, wet wool, acacia flower or just a soapy note⁴.

PINKING PHENOMENA ON WHITE WINES: RELATION BETWEEN PINKING SUSCEPTIBILITY INDEX (PSI) AND WINE ANTHOCYANINS CONTENT

Pinking is the emergence of pink tones in white wines exclusively produced from white grape varieties, known as pinking phenomena for many years. Pinking is essentially appeared when white wines are produced under reducing conditions [1,2,3]. Pinking usually occurs after bottling and storage of white wines, but its appearance has also been described after alcoholic fermentation or even as soon as the grape must is extracted [4]. Therefore, the purpose of this work was to investigate the existence of an-thocyanins in white wines made from different white grape varieties and grown locations and critically evaluate the most common method used for predicting pinking appearance in white wines: the Pinking Susceptibility Index (PSI).

SIP and save the planet: a sensory and consumer exploration of australian wines made from potentially drought-tolerant white wine grapes

In order to attenuate the effects of climate change on the ability to cultivate quality wine grape vines in Australia, it is essential to adapt to the projected less favourable Australian climate scenarios. One response may be to convert a portion of the current grapevine plantings to those varieties that demand less water and can tolerate increased heat. This investigation aimed to (i) generate sensory profiles and (ii) obtain knowledge about Australian wine consumers’ preferences and opinions of Australian wines made from potentially drought tolerant, white wine grape varieties not traditionally cultivated in Australia. A Rate-All-That-Apply (RATA) sensory panel (n = 49) generated sensory profiles of 44 commercial white wines made from 7 different white grape varieties (Arinto, Fiano, Garganega, Greco, Verdejo, Verdelho and Vermentino), plus two benchmark examples each of an Australian Riesling, Pinot Gris and Chardonnay wine.