terclim by ICS banner
IVES 9 IVES Conference Series 9 UNCOVERING THE ROLE OF BERRY MATURITY STAGE AND GRAPE GENOTYPE ON WINE CHARACTERISTICS: INSIGHTS FROM CHEMICAL CHARACTERISTICS AND VOLATILE COMPOUNDS ANALYSIS

UNCOVERING THE ROLE OF BERRY MATURITY STAGE AND GRAPE GENOTYPE ON WINE CHARACTERISTICS: INSIGHTS FROM CHEMICAL CHARACTERISTICS AND VOLATILE COMPOUNDS ANALYSIS

Abstract

In a climate change context and aiming for sustainable, high-quality Bordeaux wine production, this project examines the impact of grape maturity levels in various cultivars chosen for their adaptability, genetic diversity, and potential to enhance wine quality. The study explores the effects on wine composition and quality through sensory and molecular methods. We studied eight 14-year-old Vitis vinifera cv. grape varieties from the same area (VITADAPT plots 1 and 5): Cabernet Franc, Cabernet Sauvignon, Carmenère, Castets, Cot, Merlot, Petit Verdot, and Touriga Nacional. We examined three berry maturity stages from the 2022 vintage: mid-veraison (MV), mid-maturity (MM), 7 days before maturity (M-7), at maturity (M), and 10 days post-maturity (M+10). Classical composition parameters were monitored during maturation. Fine volatile compounds, including lactones, furanones, norisoprenoids, and carbonyls as ripening and over-ripening markers, were quantified in grapes and wines using SPME-GC-MS, while thiols were analyzed in wines by SPE-GC-MS/MS. For example, according to the maturity stages, a significant increase in alcohol content was observed, which varied depending on the grape genotype. The highest concentrations were found in Petit Verdot (13.78 g/L in M-7), Cabernet Sauvignon, Merlot, and Petit Verdot (15.21, 15.30, and 15.75 g/L in M) and Merlot (16.68 g/L in M+10). These values were directly related to the higher sugar concentrations found in their must during the evaluated periods. Total acidity and pH levels vary among cultivars and are also influenced by different maturation stages. Some cultivars show more significant changes over time, while others display more modest fluctuations. As expected, the pH values and total acidity in wines from different cultivars were inversely related. Concerning the analyzed volatile compounds, surprisingly, Petit Verdot exhibited the highest concentrations of γ-nonalactone, followed by Cabernet Sauvignon and Cot, at all maturity stages including M-7 (6.39, 3.90, 3.61 µg/L), M (20.98, 8.98, 6.05 µg/L), and M+10 (13.93, 12.40, 8.48 µg/L), respectively. Overall, this study offers a new method to assess varieties’ sensitivity to overripening and vital insights into the impact of berry maturity stage and cultivar on wine physicochemical traits and volatile compound profiles. These findings can be a foundation for future research aiming to predict or model wine’s chemical and sensory properties.

 

1. Wang, Lina, et al. “Regulation of anthocyanin and sugar accumulation in grape berry through carbon limitation and exoge-nous ABA application.” Food Research International 160 (2022): 111478.
2. Pons, Alexandre, et al. “Impact of the Closure Oxygen Transfer Rate on Volatile Compound Composition and Oxidation Aroma Intensity of Merlot and Cabernet Sauvignon Blend: A 10 Year Study.” Journal of Agricultural and Food Chemistry (2022).
3. Van Leeuwen, Cornelis, et al. “How terroir shapes aromatic typicity in grapes and wines (Part I): Sourced from the research article:” Recent advancements in understanding the terroir effect on aromas in grapes and wines”(OENO One, 2020). Original language of the article: English.” IVES Technical Reviews, vine and wine (2023).
4. Darriet, Philippe, et al. “Aroma and aroma precursors in grape berry.” The biochemistry of the grape berry (2012): 111-136.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Jacqueline Santos¹, Sabine Guilhaume¹, Cécile Thibon², Alexandre Pons²

1. UMR EGFV, Bordeaux Sciences Agro, INRA, University of Bordeaux, ISVV, Villenave d’Ornon, France.
2. Unitéde recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Universitéde Bordeaux, Bordeaux INP, F33882 Villenave d’Ornon France

Contact the author*

Keywords

Volatiles compounds, SPME-GC-MS, Composition parameters, Grape maturity

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

INSIGHTS ON THE ROLE OF GENES ON AROMA FORMATION OF WINES

Yeast secondary metabolism is a complex network of biochemical pathways and the genetic profile of the yeast carrying out the alcoholic fermentation is obviously important in the formation of the metabolites conferring specific odors to wine. The aim of the present research was to investigate the relative expression of genes involved in flavor compound production in eight different Saccharomyces cerevisiae strains.
Two commercial yeast strains Sc1 (S.cerevisiae x S.bayanus) and Sc2 (S.cerevisiae) and six indigenous S. cerevisiae strains (Sc3, Sc4, Sc5, Sc6, Sc7, Sc8) isolated during spontaneous fermentations were inoculated in Assyrtiko and Vidiano grape must.

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].

DEVELOPMENT OF DISTILLATION SENSORS FOR SPIRIT BEVERAGES PRODUCTION MONITORING BASED ON IMPEDANCE SPECTROSCOPY MEASUREMENT AND PARTIAL LEAST SQUARES REGRESSION (PLS-R)

During spirit beverages production, the distillate is divided in three parts: the head, the heart, and the tail. Acetaldehyde and ethanol are two key markers which allow the correct separation of distillate. Being toxic, the elimination of the head part, which contains high concentration of acetaldehyde, is crucial to guarantee the consumer’s health and security. Plus, the tail should be separated from the heart based on ethanol concentration.

INVESTIGATION OF MALIC ACID METABOLIC PATHWAYS DURING ALCOHOLIC FERMENTATION USING GC-MS, LC-MS, AND NMR DERIVED 13C-LABELED DATA

Malic acid has a strong impact on wine pH and the contribution of fermenting yeasts to modulate its concentration has been intensively investigated in the past. Recent advances in yeast genetics have shed light on the unexpected property of some strains to produce large amounts of malic acid (“acidic strains”) while most of the wine starters consume it during the alcoholic fermentation. Being a key metabolite of the central carbohydrate metabolism, malic acid participates to TCA and glyoxylate cycles as well as neoglucogenesis. Although present at important concentrations in grape juice, the metabolic fate of malic acid has been poorly investigated.

BIOSORPTION OF UNDESIRABLE COMPONENTS FROM WINE BY YEAST-DERIVED PRODUCTS

4-Ethylphenol (EP) in wine is associated with organoleptic defects such as barn and horse sweat odors. The origin of EP is the bioconversion reaction of p-coumaric acid (CA), naturally present in grapes and grape musts by contaminating yeasts of the genus Brettanomyces bruxellensis.
Yeast cell walls (YCW) have shown adsorption capacities for different compounds. They could be applied to wines in order to adsorb either CA and/or EP and thus reduce the organoleptic defects caused by the contaminating yeasts.