terclim by ICS banner
IVES 9 IVES Conference Series 9 CHARACTERIZATION OF ENOLOGICAL OAK TANNIN EXTRACTS BY MULTI-ANALYTICAL METHODS APPROACH

CHARACTERIZATION OF ENOLOGICAL OAK TANNIN EXTRACTS BY MULTI-ANALYTICAL METHODS APPROACH

Abstract

Oak tannin extracts are commonly used to improve wine properties. The main polyphenols found in oak wood extracts are ellagitannins¹ that release ellagic acid upon hydrolysis and comprise numerous structures². Moreover, oak tannin extracts contain other compounds giving a complex mixture. Consequently, the official OIV method based on gravimetric analysis of the tannin fraction adsorbed on polyvinylpolypyrrolidone is not sufficient to describe their composition and highlight their chemical diversity.

Eight commercial oak tannins were characterized by a combination of analytical approaches, Polyphe-nols were analyzed using the official OIV method, UV spectrophotometry, UPLC-UV-MS analysis be-fore and after acidic methanolysis, and HPLC-SEC-UV. Neutral sugars and polyols were determined as alditol acetates by GC-FID analysis, before and after hydrolysis. Protein content was estimated by the Kjeldahl method. Finally, samples were compared by a non-targeted metabolomic approach based on UHPLC−HRMS/MS.

Gravimetric analysis, absorbance values at 280 nm, and the quantities of ellagic acid released by methanolysis revealed some differences between samples, indicating variations in their tannin composition. This was confirmed by HPLC-SEC-UV analysis evidencing differences in tannin size distribution, particularly in larger polymer content.

All samples contained significant quantities of sugars, and in particular xylose, mostly found in the linked form, and of quercitol, a polyol marker of oak origin. These compounds contributed to up to 25% of the whole extract composition, the proportions of free and combined sugars and polyols also showing large variations between tannins. The protein content was very low, generally representing less than 1% of the mass. Non targeted UPLC-HRMS analysis detected major ellagitannins such as vescalagin, castalagin, and ro-burins A-E, but also a large number of derivatives as well as other molecules such as lignans and quercotriterponosides, and highlighted large differences between samples. Tannin extracts also contained aldehydes (HMF, furfural, syringaldehyde, sinapaldehyde, vanillin) in variable quantities.

This work demonstrates the variability in the composition of commercial oak tannin extracts, likely to impact their properties, and emphasizes the need for detailed multi-method characterization in the frame of quality control and selection of tannins for specific applications.

 

1. Simón, B. F. de; Cadahía, E.; Conde, E.; García-Vallejo, M. C. Ellagitannins in Woods of Spanish, French and American Oaks. 1999, 53 (2), 147–150. https://doi.org/doi:10.1515/HF.1999.024.
2. Moutounet, M.; Souquet, J.-M.; MEUDEC, E.; Leaute, B.; DELBOS, C.; Doco, T. Analyse de La Composition de Tanins Oenolo-giques. Rev. Fr. Oenologie 2004, No. 208, 22–27.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Hélène Hallea,² , Kevin Pascotto³ , Aude Watrelot1,2,4, Aurélie Roland1,2, Emmanuelle Meudec1,2, Pascale Williams 1, Stéphanie Car-rillo 1, Bertand Robillard 3, Nicolas Sommerer 1,2, Céline Poncet-Legrand 1, Véronique Cheynier 1,2

1. Univ. Montpellier, SPO, INRAE, Institut Agro Montpellier Supagro, 34070 Montpellier, France
2. INRAE, PROBE research infrastructure, PFP polyphenols analysis facility, 34070 Montpellier, France
3. Institut Œnologique de Champagne, Epernay, France
4. Iowa State University, Department of Food Science and Human Nutrition, Ames, USA

Contact the author*

Keywords

Oak extract, Ellagitanins, oses and polyols, HRMS

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

Influence of agrophotovoltaic on vine and must in a cool climate

The current energy crisis means that interest in agrophotovoltaics has increased significantly. The reason behind this is that the system aims to combine agricultural production with energy production. During the three-year period from 2020 to 2022, the effects of photovoltaic panels on the vine, the yield and the quality of the must were studied in Walenstadt in northern Switzerland, an area with a cool, humid climate. 65 Pinot noir vines were planted in the 160m2 study area. Because of the large edge effects, only 3 repetitions with 4 vines each could be created. A significantly lower leaf infestation by Plasmopara viticola was observed among the panels in each of the three years.

EFFECT OF OXIDATION ON LOW MOLECULAR WEIGHT PHENOLIC FRACTION, SALIVARY PROTEINS PRECIPITATION AND ASTRINGENCY SUBQUALITIES OF RED WINES

Changes in the low molecular weight phenolic fraction, obtained by liquid-liquid microextraction technique, were studied after controlled oxidation of two typologies of Sangiovese wines (Brunello di Montalcino and Chianti Classico) belonging to two vintages (2017 and 2018). The fractions were characterized by LC-MS and quantified by HPLC. The most abundant extracted compounds were the phenolic acids. The effect of oxidation, vintage, and wine typology was stated by a three-ways ANOVA. Gallic and syringic acids significantly increased after oxidation while (–)-epicatechin decreased the most.

INTENSE PULSED LIGHT FOR VINEYARD WASTEWATER: A PROMISING NEW PROCESS OF DEGRADATION FOR PESTICIDES

The use of pesticides for vine growing is responsible for generating an important volume of wastewater. In 2009, 13 processes were authorized for wastewater treatment but they are expensive and the toxicological impact of the secondary metabolites that are formed is not clearly established. Recently photodecomposition processes have been studied and proved an effectiveness to degrade pesticides and to modify their structures (Maheswari et al., 2010, Lassale et al., 2014). In this field, Pulsed Light (PL) seems to be an interesting and efficient process (Baranda et al., 2017). Therefore, the aim of this work was to investigate the PL technology as a new process for the degradation of pesticides.

WINE FERMENTATION METABOLITES PRODUCED BY TWO TORULASPORA DELBRUECKII STRAINS ISOLATED FROM OKANAGAN VALLEY, BC, CANADA VINEYARDS

Wine aroma is influenced by various factors, from agricultural practices in the vineyard to the enological choices made by winemakers throughout the vinification process. Spontaneous fermentations have a characteristically deeper complexity of aromas when compared to fermentations that have been inoculated with Saccharomyces (S.) cerevisiae because of the diversity of microflora naturally present on grape skins. Non-Saccharomyces yeast are being extensively studied for their ability to positively contribute to wine aroma and flavour. These yeasts are known to liberate more bound volatile compounds present in grape must than S. cerevisiae through the enzymatic action of β-glucosidases and β-lyases1.

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].