terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

Abstract

Pre-fermentative skin maceration is a technique used in white wine production to enhance varietal aroma, but it can increase protein concentration, leading to protein instability and haze formation [1]. To prevent protein instability, wine producers typically use fining agents such as bentonite, before wine bottling, which can negatively impact sensory characteristics and produce waste [2,3]. The aim of this study was to understand the impact of alternative techniques such as the application of polysaccharides (k-carrageenan and chitosan) on protein stability and on the wine macromolecular composition. The results showed that k-Carrageenan reduced the content of pathogenesis-related proteins (thaumatin-like proteins and chitinases), and consequently the protein instability in Albariño wines obtained with and without pre-fermentative skin maceration, and it was more efficient than sodium and calcium bentonites. Fungal chitosan was unable to heat stabilise both wines, and pathogenesis-related protein levels remained unchanged. Besides the impact on the protein content and wine protein instability, the use of k-carrageenan, chitosan, sodium, and calcium bentonites also differently impacted the wine polysaccharide content. Fungal chitosan decreased the wine polysaccharide content by 60%. Sodium and calcium bentonite also decreased the levels of wine polysaccharides although to a lower extent (16% to 59%) [4]. k-Carrageenan did not affect the wine polysaccharide composition. In conclusion, the results indicate that k-carrageenan is a suitable solution for white wine protein stabilisation, having a more desirable impact on the wine macromolecular fraction than the other fining agents tested, reducing the levels of the wine pathogenesis-related proteins without impacting polysaccharide composition. Funding : This work was funded by the Project AgriFood XXI (NORTE-01-0145-FEDER-000041) co-financed by the European Regional Development Fund through NORTE 2020 (Programa Operacional Regional do Norte 2014/2020).
Acknowledgments : The authors acknowledge Fundação para a Ciência e Tecnologia (FCT-Portugal) to CQ-VR (UIDB/00616/2020 and UIDP/00616/2020), Bodegas la Caña, S.L. do Grupo Jorge Ordoñez Selections for performing the vinification process of the wines used in this study, Ceamsa, Spain, for sup-plying the k-carrageenan sample used in this study and SAI Enology, Portugal, for performing the FTIR analysis.

 

1. Tian, B.; Harrison, R.; Morton, J.; Jaspers, M. Influence of skin contact and different extractants on extraction of proteins and phenolic substances in Sauvignon Blanc grape skin. Aust. J. Grape Wine Res. 2020, 26, 180–186.
2. Cosme, F.; Filipe-Ribeiro, L.; Nunes, F.M. Wine Stabilisation: An Overview of Defects and Treatments. In Chemistry and Biochemistry of Winemaking, Wine Stabilization and Aging [Working Title]; IntechOpen: London, UK, 2021
3. Cosme, F.; Fernandes, C.; Ribeiro, T.; Filipe-Ribeiro, L.; Nunes, F.M. White Wine Protein Instability: Mechanism, Quality Control and Technological Alternatives for Wine Stabilisation—An Overview. Beverages 2020, 6, 19
4. Arenas, I.; Ribeiro, M.; Filipe-Ribeiro, L.; Vilamarim, R.; Costa, E.; Siopa, J.; Cosme, F.; Nunes, F.M. Effect of Pre-Fermen-tative Maceration and Fining Agents on Protein Stability, Macromolecular, and Phenolic Composition of Albariño White Wines: Comparative Efficiency of Chitosan, k-Carrageenan and Bentonite as Heat Stabilisers. Foods 2021, 10, 608. https://doi.org/10.3390/foods10030608
5. Vidal, S., Francis, L., Guyot, S., Marnet, N., Kwiatkowski, M., Gawel, R., … Waters, E. J. (2003). The mouth-feel properties of grape and apple proanthocyanidins in a wine-like medium. Journal of the Science of Food and Agriculture, 83(6), 564–573. https://doi.org/10.1002/jsfa.1394

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Fernanda Cosme 1,2, Inma Arenas², Miguel Ribeiro², Luís Filipe-Ribeiro², Rafael Vilamarim², Elisa Costa², João Siopa², and Fernan-do M. Nunes2,3

1. Biology and Environment Department, School of Life Sciences and Environment, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal
2. Chemistry Research Centre-Vila Real (CQ-VR), Food and Wine Chemistry Lab., University of Trás-os-Montes and Alto Dou-ro, 5000-801 Vila Real, Portugal
3. Chemistry Department, School of Life Sciences and Environment, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal

Contact the author*

Keywords

Fining, Polysaccharides, Protein stability, Macromolecular composition

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

AROMA AND SENSORY CHARACTERIZATION OF XINOMAVRO RED WINES FROM DIFFERENT GREEK PROTECTED DESIGNATIONS OF ORIGIN, EFFECT OF TERROIR CHARACTERISTICS

The quality of wines has often been associated with their geographical area of production. The aim of this work was to characterize Protected Designation of Origin (PDO) Xinomavro red wines from different geographical areas of Amyndeon and Naoussa in Northern Greece, elaborated with variables that contribute to their differentiation, such as soil characteristics, altitude, monthly average temperature and rainfall.
Xinomavro fruit parcels from different vineyards within the two PDO zones (5 PDO Naoussa and 6 PDO Amyndeon) were vinified following a standard winemaking process. A total of 25 aroma compounds were quantified using gas chromatography-mass spectrometry (GC-MS) with simultaneous full scan and selected ion monitoring for data recording, and odor activity values (OAVs) were determined.

INVESTIGATION INTO MOUSY OFF-FLAVOR IN WINE USING GAS CHROMATOGRAPHY-MASS SPECTROMETRY WITH STIR BAR SORPTIVE EXTRACTION

Mousy off-flavor is one of the defects of microbial origin in wine. It is described as a particularly unpleasant defect reminiscent of rodent urine (a “dirty mouse cage”), and grilled foods such as popcorn, rice, crackers, and bread crust. Prior to the 2010s, mousiness was very uncommon but it has been becoming more frequent in recent years. It is often associated with an increase in pH as well as certain oenological practices, which tend to significantly decrease the use of sulfur dioxide.

Overhead spray water treatment as a mitigation strategy for reducing vine stress and preserving grape quality during heatwaves

Changes in climate have been influencing the quality of wine grapes worldwide. The impact of extreme climate events over short periods is increasingly recognized as a serious risk to grape quality and yield quantity. In this study the mitigation effects of a pulsed water spray on vine canopy during heatwave events has been evaluated for maintaining vine condition during the growing season and grape quality. Vines of three varieties (Malbec, Bonarda, and Syrah) under drip irrigation in the UNCuyo experimental vineyard were treated with an overhead pulsed water spray.

A synthesis approach on the impact of elevated CO2 on berry physiology and yield of Vitis vinifera

Besides the increase in global mean temperature the second main challenge of a changing climate is the increase in atmospheric carbon dioxide (CO2) in relation to physiology and yield performance of grapevines. The benefits of increasing CO2 levels under greenhouse environment or open field studies have been well investigated for various annual crops. Research under free carbon dioxide enrichment on field-grown perennial plants such as grapevines is limited to a few studies. Further, chamber and greenhouse experiments have been conducted mostly on potted vines under eCO2 conditions.

INOCULATION OF THE SELECTED METSCHNIKOWIA PULCHERRIMA MP1 AS A BIOPROTECTIVE ALTERNATIVE TO SULFITES TO PREVENT BROWNING OF WHITE GRAPE MUST

Enzymatic browning (BE) of must is caused by polyphenol oxidases (PPOs), tyrosinase and laccase. Both PPOs can oxidize diphenols such as hydroxycinnamic acids (HA) to quinones, which can later polymerize to form melanins [1], which are responsible of BE in white wines and of oxidasic haze in red wines. SO₂ is the main tool used to protect must from BE thanks to its capacity to inhibit PPOs [2]. However, the current trend in winemaking is to reduce and even eliminate this unfriendly additive. Among the different possible alternatives for protecting must against BE, the inoculation with a selected Metschnikowia pulcherrima MP1 is without any doubt one of the most promising ones.