terclim by ICS banner
IVES 9 IVES Conference Series 9 IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

IMPACT OF FINING WITH K-CARRAGEENAN, BENTONITE, AND CHITOSAN ON PROTEIN STABILITY AND MACROMOLECULAR COMPOUNDS OF ALBARIÑO WHITE WINE PRODUCED WITH AND WITHOUT PRE-FERMENTATIVE SKIN MACERATION

Abstract

Pre-fermentative skin maceration is a technique used in white wine production to enhance varietal aroma, but it can increase protein concentration, leading to protein instability and haze formation [1]. To prevent protein instability, wine producers typically use fining agents such as bentonite, before wine bottling, which can negatively impact sensory characteristics and produce waste [2,3]. The aim of this study was to understand the impact of alternative techniques such as the application of polysaccharides (k-carrageenan and chitosan) on protein stability and on the wine macromolecular composition. The results showed that k-Carrageenan reduced the content of pathogenesis-related proteins (thaumatin-like proteins and chitinases), and consequently the protein instability in Albariño wines obtained with and without pre-fermentative skin maceration, and it was more efficient than sodium and calcium bentonites. Fungal chitosan was unable to heat stabilise both wines, and pathogenesis-related protein levels remained unchanged. Besides the impact on the protein content and wine protein instability, the use of k-carrageenan, chitosan, sodium, and calcium bentonites also differently impacted the wine polysaccharide content. Fungal chitosan decreased the wine polysaccharide content by 60%. Sodium and calcium bentonite also decreased the levels of wine polysaccharides although to a lower extent (16% to 59%) [4]. k-Carrageenan did not affect the wine polysaccharide composition. In conclusion, the results indicate that k-carrageenan is a suitable solution for white wine protein stabilisation, having a more desirable impact on the wine macromolecular fraction than the other fining agents tested, reducing the levels of the wine pathogenesis-related proteins without impacting polysaccharide composition. Funding : This work was funded by the Project AgriFood XXI (NORTE-01-0145-FEDER-000041) co-financed by the European Regional Development Fund through NORTE 2020 (Programa Operacional Regional do Norte 2014/2020).
Acknowledgments : The authors acknowledge Fundação para a Ciência e Tecnologia (FCT-Portugal) to CQ-VR (UIDB/00616/2020 and UIDP/00616/2020), Bodegas la Caña, S.L. do Grupo Jorge Ordoñez Selections for performing the vinification process of the wines used in this study, Ceamsa, Spain, for sup-plying the k-carrageenan sample used in this study and SAI Enology, Portugal, for performing the FTIR analysis.

 

1. Tian, B.; Harrison, R.; Morton, J.; Jaspers, M. Influence of skin contact and different extractants on extraction of proteins and phenolic substances in Sauvignon Blanc grape skin. Aust. J. Grape Wine Res. 2020, 26, 180–186.
2. Cosme, F.; Filipe-Ribeiro, L.; Nunes, F.M. Wine Stabilisation: An Overview of Defects and Treatments. In Chemistry and Biochemistry of Winemaking, Wine Stabilization and Aging [Working Title]; IntechOpen: London, UK, 2021
3. Cosme, F.; Fernandes, C.; Ribeiro, T.; Filipe-Ribeiro, L.; Nunes, F.M. White Wine Protein Instability: Mechanism, Quality Control and Technological Alternatives for Wine Stabilisation—An Overview. Beverages 2020, 6, 19
4. Arenas, I.; Ribeiro, M.; Filipe-Ribeiro, L.; Vilamarim, R.; Costa, E.; Siopa, J.; Cosme, F.; Nunes, F.M. Effect of Pre-Fermen-tative Maceration and Fining Agents on Protein Stability, Macromolecular, and Phenolic Composition of Albariño White Wines: Comparative Efficiency of Chitosan, k-Carrageenan and Bentonite as Heat Stabilisers. Foods 2021, 10, 608. https://doi.org/10.3390/foods10030608
5. Vidal, S., Francis, L., Guyot, S., Marnet, N., Kwiatkowski, M., Gawel, R., … Waters, E. J. (2003). The mouth-feel properties of grape and apple proanthocyanidins in a wine-like medium. Journal of the Science of Food and Agriculture, 83(6), 564–573. https://doi.org/10.1002/jsfa.1394

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Fernanda Cosme 1,2, Inma Arenas², Miguel Ribeiro², Luís Filipe-Ribeiro², Rafael Vilamarim², Elisa Costa², João Siopa², and Fernan-do M. Nunes2,3

1. Biology and Environment Department, School of Life Sciences and Environment, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal
2. Chemistry Research Centre-Vila Real (CQ-VR), Food and Wine Chemistry Lab., University of Trás-os-Montes and Alto Dou-ro, 5000-801 Vila Real, Portugal
3. Chemistry Department, School of Life Sciences and Environment, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal

Contact the author*

Keywords

Fining, Polysaccharides, Protein stability, Macromolecular composition

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

WINE RACKING IN THE WINERY AND THE USE OF INERT GASES: CONTROL AND OPTIMIZATION OF THE PROCESS

Atmospheric oxygen (O₂) generates oxidation in wines that affect their physicochemical and sensory evolution. The O₂ uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O₂ uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The aim was to study the uptake of O₂ during the racking of a model wine as a reference and to compare with purging the destination tank with different inert gases.

FREE TERPENE RESPONSE OF ‘MOSCATO BIANCO’ VARIETY TO GRAPE COLD STORAGE

Temperature control is crucial in wine production, starting from grape harvest to the bottled wine storage. Climate change and global warming affect the timing of grape ripening, and harvesting is often done during hot summer days, influencing berry integrity, secondary metabolites potential, enzyme and oxidation phenomena, and even fermentation kinetics. To curb this phenomenon, pre-fermentative cold storage can help preserve the grapes and possibly increase the concentration of key secondary metabolites. In this study, the effect of grape pre-fermentative cold storage was assessed on the ‘Moscato bianco’ white grape cultivar, known for its varietal terpenes (65% of free terpenes represented by linalool and its derivatives) and widely used in Piedmont (Italy) to produce Asti DOCG wines.

INCREASING PINOT NOIR COLOUR DENSITY THROUGH SEQUENTIAL INOCULATION OF FLOCCULENT COMMERCIAL WINE YEAST SPECIES

Vitis vinifera L. cv. Pinot noir can be challenging to manage in the winery as its thin skins require careful handling to ensure sufficient extraction of wine colour to promote colour stability during ageing.1 Literature has shown that fermentation with flocculent yeasts can increase red wine colour density.2 As consumers prefer greater colour density in red wines,3 the development of tools to increase colour density would be useful for the wine industry. This research explored the impact of interspecies sequential inoculation and co-flocculation of commercial yeast on Pinot noir wine colour.

UNRAVELLING THE ROLE OF LACTIC ACID BACTERIA ON SPARKLING WINE ELABORATION THROUGH METABOLOMICS APPROACH

Xinomavro is a red grape variety from Northern Greece (Protected Designation of Origin), known for the nice acidities, perfectly appropriate for sparkling wine production (Rosé and Blanc de Noir). The elabo- ration of sparkling wine requires technical as well as scientific skills. Although the impact of the yeast strains and their metabolites on the final product quality is well documented, the action of bacteria still remains unknown.
The present work focuses (i) on the population diversity of lactic acid bacteria isolated from sparkling wines and (ii) on the technological effect of the species during sparkling wine elaboration.

INVESTIGATION OF MALIC ACID METABOLIC PATHWAYS DURING ALCOHOLIC FERMENTATION USING GC-MS, LC-MS, AND NMR DERIVED 13C-LABELED DATA

Malic acid has a strong impact on wine pH and the contribution of fermenting yeasts to modulate its concentration has been intensively investigated in the past. Recent advances in yeast genetics have shed light on the unexpected property of some strains to produce large amounts of malic acid (“acidic strains”) while most of the wine starters consume it during the alcoholic fermentation. Being a key metabolite of the central carbohydrate metabolism, malic acid participates to TCA and glyoxylate cycles as well as neoglucogenesis. Although present at important concentrations in grape juice, the metabolic fate of malic acid has been poorly investigated.