terclim by ICS banner
IVES 9 IVES Conference Series 9 FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

FUNCTIONALIZED MESOPOROUS SILICA IS A VIABLE ALTERNATIVE TO BENTONITE FOR WINE PROTEIN STABILIZATION

Abstract

The presence of grape-derived heat unstable proteins can lead to haze formation in white wines [1], an instability prevented by removing these proteins by adding bentonite, a hydrated aluminum silicate that interacts electrostatically with wine proteins leading to their flocculation. Despite effective, using bentonite has several drawbacks as the costs associated with its use, the potential negative effects on wine quality, and its environmental impact, so that alternative solutions are needed.

This project aimed at evaluating the effectiveness of functionalized mesoporous silica (FMS), in removing heat unstable proteins from white musts and wines. FMS treatments were benchmarked against a commercial Na-Bentonite in a series of experiments conducted on heat unstable white musts and wines of different origin, vintage and variety, and on different scales (from few mL to 10 hL). The stabilizing properties of the fining agents were determined by analyzing the protein profiles of treated wines (by RP-HPLC), and by assessing protein stability via heat tests [3]. In addition, the treatments’ impact on other wine parameters (e.g., organic acid profiles, metal content, macromolecules, lees formation, sensory analysis) were determined.

For each wine, the dose of bentonite and FMS needed to reach full protein stability was determined by fining rate trials. The amount of FMS needed to stabilize the wines was always in line with that of bentonite, with a small variability (±10%) attributable to differences in wine composition. FMS effectively removed both thaumatin-like proteins and chitinases in a dose dependent mode, without causing other modifications on wine composition in terms of organic acid profile, ethanol content, glycerol, volatile composition, and metal content that, on the other hand, was always modified by bentonite fining that always led to an increase in Fe and Al. The analysis by triangle test of two white wines (Sauvignon blanc and Traminer) stabilized with FMS or bentonite at similar addition rates revealed the lack of significant differences (total answers = 39, p = 0. 5599 for S. blanc, p = 0.1184 for Traminer). In general, FMS showed to effectively stabilized wines at addition rates similar to those of bentonite, without causing major compositional modification, nor detectable sensory impacts, and therefore they represent a good candidate to become a viable bentonite alternative.

 

1. Van Sluyter, S.C.; McRae, J.M.; Falconer, R.J.; Smith, P.A.; Bacic, A.; Waters, E.J.; Marangon, M. Wine Protein Haze: Mechanisms of Formation and Advances in Prevention. J. Agric. Food Chem. 2015, 63, 4020–4030.
2. Lambri, M.; Dordoni, R.; Silva, A.; Faveri, D.M.D. Effect of Bentonite Fining on Odor-Active Compounds in Two Different White Wine Styles. Am. J. Enol. Vitic. 2010, 61:2, 225–233.
3. McRae, J.M.; Barricklow, V.; Pocock, K.F.; Smith, P.A. Predicting Protein Haze Formation in White Wines. Aust. J. Grape Wine Res. 2018, 24, 504–511.

DOI:

Publication date: February 9, 2024

Issue: OENO Macrowine 2023

Type: Poster

Authors

Matteo Marangon1,2, Lucia Bernardi¹, Edward Brearley-Smith¹, Christine Mayr Marangon¹, Fabio Angiuli³, Stefano Caramori³, Roberto Argazzi⁴, Gianni Triulzi⁵, Alessandra Basana⁵

1. Department of Agronomy, Food, Natural Resources Animals and Environment (DAFNAE), University of Padua, Viale dell’Uni-versità, 16, 35020 Legnaro (PD), Italy
2. Interdepartmental Centre for Research in Viticulture and Enology (CIRVE), University of Padova, 31015 Conegliano, Italy
3. Department of Chemical, Pharmaceutical and Agricultural Sciences University of Ferrara Via L.Borsari 46, 44121, Ferrara, Italy
4. CNR-ISOF c/o Department of Chemical, Pharmaceutical and Agricultural Sciences University of Ferrara Via L.Borsari 46, 44121, Ferrara, Italy
5. Enartis – ESSECO srl, Via San Cassiano 99, 28069 Trecate NO, Italy

Contact the author*

Keywords

protein, stability, bentonite, wine

Tags

IVES Conference Series | oeno macrowine 2023 | oeno-macrowine

Citation

Related articles…

HOLISTIC APPROXIMATION OF THE INFLUENCE OF SACCHAROMYCES STRAINS ON WINE AROMA PRECURSORS

Wine varietal aroma is the result of a mixture of compounds formed or liberated from specific grape-aroma precursors. Their liberation/formation from their specific precursors can occur spontaneously by acid catalyzed rearrangements or hydrolysis or by the action of the yeast enzymatic activities. The influence of yeast during fermentation on the production of these volatile compounds has been widely studied however, the effect of this influence during aging is not fully understood. In order to evaluate these processes several indirect strategies have been used to study aroma precursors although they are not useful to understand the chemistry of the process.

EUGENOL AS QUALITY MARKER OF WINES AND SPIRITS FROM HYBRID VINES: IMPACT OF DIFFERENT WINEMAKING AND DISTILLATION PROCESSES

Eugenol, widely spread in various plants notably cloves, basil and bay, was identified too in wines from hybrid grapes without contact with oak wood. This aromatic molecule presents a strong spicy note of clove and also antifongic properties. Eugenol was described as an endogenous compound of Baco blanc, from the grapes to the spirits of Armagnac area. Moreover, this compound is a chemical marker of Baco blanc products quality.
Influences of harvest time and different winemaking processes (settling, use of enzymatic preparations, lees content and stock time before distillation) on Baco blanc wine eugenol contents were explored using a two-levels full factorial Design of Experiments (DoEs).

PHENOLICS DYNAMICS OF BERRIES FROM VITIS VINIFERA CV SYRAH GRAFTED ON TWO CONTRASTING ROOTSTOCKS UNDER COMBINED SALINITY AND WATER STRESSORS AND ITS EFFECT ON WINE QUALITY

Wine regions are getting warmer as average temperatures continue raising affecting grape growth, berry composition and wine production. Berry quality was evaluated in plants of Vitis vinifera cv Syrah grafted on two rootstocks, Paulsen (PL1103) and SO4, and grown under two salinity concentrations (LS:0.7dS/m and HS:2.5dSm-1) in combination with two irrigation regimes (HW:133% and CW:100%), being the seasonal water application 483mm (control, 100%). Spectrophotometer measurements from berry skin during veraison and harvest stages and from “young” wine samples, were indicative of the stressors effect and the mediation of the rootstocks. At veraison (i) total phenolics content were high under LSHW (0.7dSm-1 and high water conditions) for SO4 and PL1103.

INCREASING PINOT NOIR COLOUR DENSITY THROUGH SEQUENTIAL INOCULATION OF FLOCCULENT COMMERCIAL WINE YEAST SPECIES

Vitis vinifera L. cv. Pinot noir can be challenging to manage in the winery as its thin skins require careful handling to ensure sufficient extraction of wine colour to promote colour stability during ageing.1 Literature has shown that fermentation with flocculent yeasts can increase red wine colour density.2 As consumers prefer greater colour density in red wines,3 the development of tools to increase colour density would be useful for the wine industry. This research explored the impact of interspecies sequential inoculation and co-flocculation of commercial yeast on Pinot noir wine colour.

EVOLUTION OF CHEMICAL AND SENSORIAL PROFILE OF WINES ELABORATED WITH THEIR OWN TOASTED VINE-SHOOTS AND MICRO-OXYGENATION

The positive contribution of toasted vine-shoots (SEGs, Shoot from vines – Enological – Granule) used in winemaking to the chemical and sensory profile of wines has been widely proven. However, the combination of this new enological tool with other winemaking technologies, such as micro-oxygenation (MOX), has not been studied so far. It is known that micro-oxygenation is used in wineries to stabilizes color, improves structure or combining with oak alternatives products to achieve a more effective aroma integration of wines. For that, its implementation in combination with SEGs could result in differentiated wines.