GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Characterization of simple polyphenols in seeds of autochthonous grapevine varieties grown in Croatia (Vitis vinifera L.)

Characterization of simple polyphenols in seeds of autochthonous grapevine varieties grown in Croatia (Vitis vinifera L.)

Abstract

Context and purpose of the study – Croatia has rich grapevine genetic resources with more than 125 autochthonous varieties preserved. Coastal region of Croatia, Dalmatia, is well known for wine production based on autochthonous grapevine varieties. Nevertheless, only couple of these are widely cultivated and have greater economic importance. Grape seeds are sources of polyphenols which play an important role in organoleptic and nutritional value of grape and wine. Hence, the aim of this study was to evaluate the simple polyphenols from grape seeds in 20 rare autochthonous grapevine varieties.

Material and methods – Samples were collected during two consecutive years (2011. and 2012.) from germplasm collection in Split (Dalmatia). Grape samples were constituted of five bunches of fully ripe grapes. Seeds were manually separated, freeze-dried, grounded and stored at a low temperature until analyses. Polyphenolic compounds were analysed using HPLC analysis.

Results – Eight polyphenolic compounds, galic acid, monomeric flavan-3-ols (catechin, epicatechin, gallocatechin and epicatechin 3-O-gallate) and procyanidin dimers (B1, B2 and B4) were detected. According to the investigated polyphenolic compounds significant differences between investigated varieties were found. Gallic acid content ranged from 91.0 to 245.08 total monomeric flavan-3-ols from 619.2 to 13539.6 mg kg-1 and total procyanidin dimers from 975.3 to 4140.2 mg kg-1 of seed. Catechin (263.2 to 8124.2 mg kg-1 seed) was found as main monomeric flavan-3-ol, epicatechin 3-O-gallate, gallocatchin and epicatechin varied between 0-164.31, 37.19-155.07 and 277.5-5224.4 mg kg-1 seed, respectively. Procyanidin B2 (420.2 to 2207.8 mg kg-1 seed) was found as a main procyanidin dimer. Procyanidin B1 and B4 amount varied between 401.80-165.19 and 276,7-1539.4 mg kg-1 seed, respectively. Gegić had lowest and varieties Plavac mali and Babić highest amount of all investigated polyphenolic compounds. This study presents the first evaluation of Croatian grapevine varieties by characterization of seed polyphenolic compounds and it shows huge variability among them. More detailed analysis of polyphenolic compounds in selected varieties are carry out in our further research activities.

DOI:

Publication date: March 11, 2024

Issue: GiESCO 2019

Type: Poster

Authors

Željko ANDABAKA1, Edi MALETIĆ1,2, Domagoj STUPIĆ1, Darko PREINER1,2, Jasminka KAROGLAN KONTIĆ1,2, Ivana TOMAZ1, Iva ŠIKUTEN1, Petra ŠTAMBUK2, Zvjezdana MARKOVIĆ1*

1 University of Zagreb Faculty of Agriculture, Svetošimunska 25, 10 000 Zagreb, Croatia
2 Centre of Excellence for Biodiversity and Molecular Plant Breeding, Svetošimunska 25, HR-10000 Zagreb

Contact the author

Keywords

Grapevine, Autochthonous, Polyphenols, Seed, Croatia

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

Climate, Viticulture, and Wine … my how things have changed!

The planet is warmer than at any time in our recorded past and increasing greenhouse emissions and persistence in the climate system means that continued warming is highly likely. Climate change has already altered the basic framework of growing grapes for wine production worldwide and will likely continue to do so for years to come. The wine sector can continue to play an important role in leading the agricultural sector in addressing climate change. From developing on…

Low-cost sensors as a support tool to monitor soil-plant heat exchanges in a Mediterranean vineyard

Mediterranean viticulture is increasingly exposed to more frequent extreme conditions such as heat waves. These extreme events co-occur with low soil water content, high air vapor pressure deficit and high solar radiant energy fluxes and result in leaf and berry sunburn, lower yield, and berry quality, which is a major constraint for the sustainability of the sector. Grape growers must find ways to proper and effectively manage heat waves and extreme canopy and berry temperatures. Irrigation to keep soil moisture levels and enable adequate plant turgor, and convective and evaporative cooling emerged as a key tool to overcome this major challenge. The effects of irrigation on soil and plant water status are easily quantifiable but the impact of irrigation on soil and canopy temperature and on heat convection from soil to cluster zone remain less characterized. Therefore, a more detailed quantification of vineyard heat fluxes is highly relevant to better understand and implement strategies to limit the effects of extreme weather events on grapevine leaf and berry physiology and vineyards performance. Low-cost sensor technologies emerge as an opportunity to improve monitoring and support decision making in viticulture. However, validation of low-cost sensors is mandatory for practical applicability. A two-year study was carried in a vineyard in Alentejo, south of Portugal, using low-cost thermal cameras (FLIR One, 80×60 pixels and FLIR C5, 160×120 pixels, 8-14 µm, FLIR systems, USA) and pocket thermohygrometers (Extech RHT30, EXTECH instruments, USA) to monitor grapevine and soil temperatures. Preliminary results show that low-cost cameras can detect severe water stress and support the evaluation of vertical canopy temperature variability, providing information on soil surface temperature. All these thermal parameters can be relevant for soil and crop management and be used in decision support systems.

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.