GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 Impact of deficit irrigation strategies on terpene concentration in Gewürztraminer grapes

Impact of deficit irrigation strategies on terpene concentration in Gewürztraminer grapes

Abstract

Context and purpose of the study – Deficit irrigation is a viticultural practice often applied to improve the phenolic composition of red grapes and wines. However, the impact of this practice on grape terpenes – key aromatics for several grapes and wines – remains largely unknown. This study investigated the impact of deficit irrigation strategies on free and glycosylated terpenes in Gewürztraminer grapes.

Material and methods – In a field study conducted in Oliver, BC, in 2016, 2017, and 2018, deficit irrigation regimes were applied to Gewürztraminer vines at different developmental stages (pre-veraison = Early Deficit, ED; post-veraison = Late Deficit, LD; throughout the season = Prolonged Deficit, PD). A well-irrigated control (CN) treatment was also established. Treatments were replicated four times accordingly to a randomized block design. The impact of deficit irrigation treatments on vine physiology and berry metabolism was characterized with eco-physiological, biochemical, and molecular analyses. Starting three weeks after fruit set, midday-leaf water potential was measured every 7-14 days and leaf gas exchanges every 14-21 days. Berry samplings were conducted every 7-14 days to assess the effect of deficit irrigation treatments on berry sugar (total soluble solid, TSS), acid (titratable acidity, TA), and terpene concentration, as well as the expression of terpene genes. Free and glycosylated terpenes were identified and quantified using a SPME-GC-MS and a LI-GC-MS, respectively. Gene expression was analyzed using a quantitative RT-PCR.

Results – Midday leaf water potential, photosynthesis, and transpiration rates were reduced by deficit irrigation. ED, LD, and PD reduced vine yield when compared to CN, but LD reduction was small and not consistent among years. Sugar levels were also reduced by deficit irrigation, particularly by LD and PD. Total free terpenes were marginally affected by deficit irrigation treatments (P = 0.065); however, the concentration of specific terpenes, such as geraniol, was significantly (P< 0.05) higher in LD than in CN berries. LD did not increase the expression of terpene genes (e.g., VviDXSs, VviHDR, VviTPSs), suggesting that the observed increase in the concentration of some free terpenes was not regulated at the transcriptional level. Total glycosylated terpenes at harvest did not change among treatments.

DOI:

Publication date: March 12, 2024

Issue: GiESCO 2019

Type: Poster

Authors

Yevgen KOVALENKO1, Ricco TINDJAU1, Simone Diego CASTELLARIN1

1 Wine Research Centre, The University of British Columbia, 2205 East Mall, Vancouver, BC, V6T0C1, Canada

Contact the author

Keywords

Aroma, Grapevine, Ripening, Water Deficit, Yield

Tags

GiESCO | GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Influence of harvest time and withering length combination on reinforced Nebbiolo wines: phenolic composition, colour traits, and sensory profile

Sforzato di Valtellina DOCG is a reinforced dry red wine produced in the mountain area of Valtellina alpine valley (North Italy), using ‘Nebbiolo’ grapes that undergo a withering process. This process impacts on the grape composition due to a sugar concentration and changes in secondary metabolism influencing volatile organic compounds (VOCs) and polyphenols.

The effects of cane girdling on berry texture properties and the concentration of some aroma compounds in three table grape cultivars

The marketability of the table grapes is highly influenced by the consumer demand; therefore the market value of the table grapes is mainly characterized by its berry size, colour, taste and texture. Girdling could cause accumulation of several components in plants above the ringing of the phloem including clusters and resulting improved maturity. The aim of the experiments was to examine the effect of girdling on berry texture characteristics and aroma concentration.

HRATA : A new sensory methodology using advantage of wine aromatic wheels

Wine is an intrinsically complex aromatic product. To formalize this aromatic diversity and the hierarchical structure of the aromas, it is common to present them in the form of a wheel of aromas. These are used for learning and communication purposes but never for the acquisition of sensory characteristics.

Comparison of imputation methods in long and varied phenological series. Application to the Conegliano dataset, including observations from 1964 over 400 grape varieties

A large varietal collection including over 1700 varieties was maintained in Conegliano, ITA, since the 1950s. Phenological data on a subset of 400 grape varieties including wine grapes, table grapes, and raisins were acquired at bud break, flowering, veraison, and ripening since 1964. Despite the efforts in maintaining and acquiring data over such an extensive collection, the data set has varying degrees of missing cases depending on the variety and the year. This is ubiquitous in phenology datasets with significant size and length. In this work, we evaluated four state-of-the-art methods to estimate missing values in this phenological series: k-Nearest Neighbour (kNN), Multivariate Imputation by Chained Equations (mice), MissForest, and Bidirectional Recurrent Imputation for Time Series (BRITS). For each phenological stage, we evaluated the performance of the methods in two ways. 1) On the full dataset, we randomly hold-out 10% of the true values for use as a test set and repeated the process 1000 times (Monte Carlo cross-validation). 2) On a reduced and almost complete subset of varieties, we varied the percentage of missing values from 10% to 70% by random deletion. In all cases, we evaluated the performance on the original values using normalized root mean squared error. For the full dataset we also obtained performance statistics by variety and by year. MissForest provided average errors of 17% (3 days) at budbreak, 14% (4 days) at flowering, 14.5% (7 days) at veraison, and 17% (3 days) at maturity. We completed the imputations of the Conegliano dataset, one of the world’s most extensive and varied phenological time series and a steppingstone for future climate change studies in grapes. The dataset is now ready for further analysis, and a rigorous evaluation of imputation errors is included.

Exploring the influence of terroir on the sensorial and aroma profiles of wines – An application to red wines from AOC Corbières

The aromatic profile of a wine is the result of volatile molecules present in grapes (varietal or primary aromas) and those produced during the winemaking process of fermentation (secondary aromas) and during wine aging (tertiary aromas).