Macrowine 2021
IVES 9 IVES Conference Series 9 Elicitors used as a tool to increase stilbenes in grapes and wines

Elicitors used as a tool to increase stilbenes in grapes and wines

Abstract

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001). Grapevine phytoalexins (plant metabolites with antimicrobial activity that are synthetized de novo and fuction as the basis of a disease resistance mechanism) are stilbene compounds synthetized and accumulated in leaves and berries in response to abiotic stresses. Numerous experimental trials have recently been conducted with diferent elicitors to promote stilbene synthesis in grapevine berries, such UV irradiation (Cantos et al. 2003; Langcake and Pryce, 1977), aluminium chloride (Adrian et al. 1996), ozone (González-Barrio et al. 2006), methyl jasmonate (Vezulli et al. 2007) and benzothiadiazole (Iriti et al. 2004) . Since a highest presence of stilbenes in grapes could be a protecion against mould infection, the application of these compounds could, not only improve the healthiness of grapes, but also limit the use of fungices. The stilbenes found in wine occur mainly in the skin of grape berries, and they pass from grape to wine during alcoholic fermentation, so, at the same time, the wines elaborated with these grapes would be considered healthier wines. In our study, five preharvest treatments considered as elilicitors, were applied in Monastrell grapes by means five elicitors: benzothiadiazole, methyljasmonate, shell chitosan, fungal chitosan and cell wall yeast. These compounds were applied as sprays on clusters at veraison and one week later. Our main objetive was to check wether these treatments could enhance stilbene accumulation in berries at the moment of harvest, and then to be extracted during winemaking. For this target, we identified, quantified and compared the stilbene content in grapes and wines elaborated with grapes from the diferent treatments against grapes and wines without no treatment consdered as a control. The results showed that only some of these compounds improved significantly the stilbene concentration in grapes but almost of them increasing their stilbene composition at the end of alcoholic fermentation, mainly trans-resveratrol which is considered as the highest biological value.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Rocío Gil*, Encarna Gómez-Plaza, Jose Ignacio Fernandez

*IMIDA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Effect of ageing with Specific Inactivated Dry Yeasts on the volatile composition of Sauvignon Blanc and Carménère wines

Úbeda-Aguilera, C a, b, Peña-Neira, A.b Del Barrio-Galán, R.b, c a Biomedical Sciences Institute, Science Faculty, Universidad Autónoma de Chile, Chile. b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile c Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las Condes, Santiago, Chile The wine is a complex matrix made up of several compounds which can interact among themselves throughout the wine ageing process, thereby modifying their sensorial characteristics. It is well known that during ageing of wines on lees, polysaccharides (mainly mannoproteins) can be released and can interact with the aromatic fraction modifying its volatility.

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols.