Macrowine 2021
IVES 9 IVES Conference Series 9 Elicitors used as a tool to increase stilbenes in grapes and wines

Elicitors used as a tool to increase stilbenes in grapes and wines

Abstract

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001). Grapevine phytoalexins (plant metabolites with antimicrobial activity that are synthetized de novo and fuction as the basis of a disease resistance mechanism) are stilbene compounds synthetized and accumulated in leaves and berries in response to abiotic stresses. Numerous experimental trials have recently been conducted with diferent elicitors to promote stilbene synthesis in grapevine berries, such UV irradiation (Cantos et al. 2003; Langcake and Pryce, 1977), aluminium chloride (Adrian et al. 1996), ozone (González-Barrio et al. 2006), methyl jasmonate (Vezulli et al. 2007) and benzothiadiazole (Iriti et al. 2004) . Since a highest presence of stilbenes in grapes could be a protecion against mould infection, the application of these compounds could, not only improve the healthiness of grapes, but also limit the use of fungices. The stilbenes found in wine occur mainly in the skin of grape berries, and they pass from grape to wine during alcoholic fermentation, so, at the same time, the wines elaborated with these grapes would be considered healthier wines. In our study, five preharvest treatments considered as elilicitors, were applied in Monastrell grapes by means five elicitors: benzothiadiazole, methyljasmonate, shell chitosan, fungal chitosan and cell wall yeast. These compounds were applied as sprays on clusters at veraison and one week later. Our main objetive was to check wether these treatments could enhance stilbene accumulation in berries at the moment of harvest, and then to be extracted during winemaking. For this target, we identified, quantified and compared the stilbene content in grapes and wines elaborated with grapes from the diferent treatments against grapes and wines without no treatment consdered as a control. The results showed that only some of these compounds improved significantly the stilbene concentration in grapes but almost of them increasing their stilbene composition at the end of alcoholic fermentation, mainly trans-resveratrol which is considered as the highest biological value.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Rocío Gil*, Encarna Gómez-Plaza, Jose Ignacio Fernandez

*IMIDA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Metabolomics of grape polyphenols as a consequence of post-harvest drying: on-plant dehydration vs warehouse withering

A method of suspect screening analysis to study grape metabolomics, was developed [1]. By performing ultra-high performance liquid chromatography (UHPLC) – high-resolution mass spectrometry (HRMS) analysis of the grape extract, averaging 320-450 putative grape compounds are identified which include mainly polyphenols. Identification of metabolites is performed by a new HRMS-database of putative grape and wine compounds expressly constructed (GrapeMetabolomics) which currently includes around 1,100 entries.

Directed Evolution of Oenococcus oeni: optimising yeast-bacteria interactions for improved malolactic fermentation

Malolactic fermentation (MLF) is a secondary step in the vinification process and it follows alcoholic fermentation (AF) which is predominantly carried out by Saccharomyces cerevisiae. These two processes result in the degradation of metabolites to produce secondary metabolites which also contribute to the final wine flavour and quality. AF results in the production of ethanol and carbon dioxide from sugars and MLF stems from the degradation of L-malic acid (a dicarboxylic acid) to L-lactic acid (a monocarboxylic acid). The latter process results in a smoother texture as the acidity of the wine is reduced by the process, it also adds to the flavour complexity of the wine.

A preliminary study of clonal selection in cv. Viura in relation to varietal aroma profile

Viura is a synonym for Macabeo and currently it is the most widely planted white grape variety in D.O.Ca. Rioja, with 3,569 ha, representing 84% of the white grape cultivated area. It is a generous-yielding grape, presenting low values of titratable acidity and with large and compact clusters which makes it susceptible to Botrytis cinerea. Thus, this variety not always satisfies the wine grower’s prospects. Nowadays, the available plant material is scarce, moreover, it was selected on the basis of other quality criteria, not currently requested.