Macrowine 2021
IVES 9 IVES Conference Series 9 Elicitors used as a tool to increase stilbenes in grapes and wines

Elicitors used as a tool to increase stilbenes in grapes and wines

Abstract

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001). Grapevine phytoalexins (plant metabolites with antimicrobial activity that are synthetized de novo and fuction as the basis of a disease resistance mechanism) are stilbene compounds synthetized and accumulated in leaves and berries in response to abiotic stresses. Numerous experimental trials have recently been conducted with diferent elicitors to promote stilbene synthesis in grapevine berries, such UV irradiation (Cantos et al. 2003; Langcake and Pryce, 1977), aluminium chloride (Adrian et al. 1996), ozone (González-Barrio et al. 2006), methyl jasmonate (Vezulli et al. 2007) and benzothiadiazole (Iriti et al. 2004) . Since a highest presence of stilbenes in grapes could be a protecion against mould infection, the application of these compounds could, not only improve the healthiness of grapes, but also limit the use of fungices. The stilbenes found in wine occur mainly in the skin of grape berries, and they pass from grape to wine during alcoholic fermentation, so, at the same time, the wines elaborated with these grapes would be considered healthier wines. In our study, five preharvest treatments considered as elilicitors, were applied in Monastrell grapes by means five elicitors: benzothiadiazole, methyljasmonate, shell chitosan, fungal chitosan and cell wall yeast. These compounds were applied as sprays on clusters at veraison and one week later. Our main objetive was to check wether these treatments could enhance stilbene accumulation in berries at the moment of harvest, and then to be extracted during winemaking. For this target, we identified, quantified and compared the stilbene content in grapes and wines elaborated with grapes from the diferent treatments against grapes and wines without no treatment consdered as a control. The results showed that only some of these compounds improved significantly the stilbene concentration in grapes but almost of them increasing their stilbene composition at the end of alcoholic fermentation, mainly trans-resveratrol which is considered as the highest biological value.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Rocío Gil*, Encarna Gómez-Plaza, Jose Ignacio Fernandez

*IMIDA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of sulfur compounds to the antioxidant stability of white wines

The chemical mechanisms involved in oxidation/reduction potential of wine during winemaking and aging are affecting its color, aroma and taste. Chemical oxidation is one of the major causes of development of off-flavors during ageing1. Thus, the chemical changes in wine during storage should be controlled to ensure the sensory quality of the product and avoid consumer rejection that will compromise the economic value of the product. The 1-hydroxyethyl radical has been recognized as the key radical intermediate in the oxidative reactions in wine2. Based on the kinetic study of POBN-1-hydroxyethyl spin adduct formation in wines initiated via the Fenton reaction, a novel tool was recently developed in our laboratory to quantify the resistance of wines against oxidation3.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.

Testing the effectiveness of Cell-Wall material from grape pomace as fining agent for red wines

Lately several works highlighted the capacity of grape cell-wall material (CWM) to interact with proanthocyanidins (PA), indicating its potential use as fining agent for red wines.1–4 However, those studies were performed by using purified PAs and very high doses of CWM (almost ten-fold higher than those used in wine industry for other commercial fining agents). The present study focuses on the applicability of CWM from Cabernet sauvignon pomace as fining agent for red wines under real winery conditions. Grapes of cultivar Cabernet sauvignon were harvested at three different maturity levels
(unripe, mature, and overripe) and used for red winemaking. The pomace of such vinifications were used as source of CWM, and applied into red wines at two different concentrations: 0.2 g/L and 2.5 g/L.

Correlations between sensory characteristics and colloidal content in dry white wines

Must clarification is an important step occurring just after grape extraction in the elaboration of white wine, consisting in a solid-liquid separation. Traditionally, low must turbidity, around 50-150 NTU, is generally reached in white winemaking in order to prevent reductive aromas and facilitating alcoholic fermentation. Alternatively, a higher turbidity (300 NTU or above) can be sought for reasons such as a better expression of grapes identity (terroir), or for getting a must matrix that could supposedly lead to wines having greater ageing potential.