Macrowine 2021
IVES 9 IVES Conference Series 9 A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

Abstract

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”. Biotechnological products are now available for effective antimicrobial actions. Lysozyme inhibits the lactic acid bacteria. Chitosan also acts on the lactic acid bacteria but also efficiently on Brettanomyces. Acid sorbic can be a help to control the development of the undesirable strains yeast strains. On the antioxidant effect oak wood could potentially play a protective role. The development of an electrochemical sensor was used to estimate the influence of different cooperage factors on the antioxydant capacity(CaOx)suitable to be transmit by the wood at the wine. For red wines, during two successive vintages in two different wineries, tests compared classical post-MLF SO2 additions (5 g/hL) in classic new barrels to a treatment with a mixture of lysozyme and chitosan in CaOx optimized barrels. Microbiological monitoring has been done like chemical assays and wines tasted by expert panels. Barrels were also fitted with an innovative device allowing to measure dissolved oxygen without to have to open the barrel. In two different “Sauternes” wineries, test have been done to compare a classic mutage (between 10 and 25 g/hL of SO2) in classic barrels to a half a dose of SO2 and adding a supplement chitosan and sorbic acid in in CaOx optimized barrels. The impact on the microbial flora were investigated as well as the impact on the quality of wine. The innovative device for measuring dissolved oxygen was also used. All these wines were followed until the end of the harvest of breeding, ie on breeding periods of 6-18 months to understand the stability over time of the results. The results show that the test wines are microbiologically more stable than control wines. In the most of the case, always for the wine experiments and often for the “liqoureux”, the dissolved oxygen levels are also lower. Some metabolites (volatile phenols, diacetyl, and acetaldehyde) are less concentrated and colors are more intense and more stable. At the end, the combination of biotechnology tools and CAOX appears of a very efficient has emerged as a very effective technique to reduce the amount of total SO2 in wine as currently requested by consumers and by legislators.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Vincent Renouf*, Marie Mirabel

*Chêne & Cie

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Primary and secondary metabolites are major components of grape quality and wine typicity. Their accumulation is interconnected through a complex metabolic network, which is still not well understood. This study aims to investigate how the enzymes of central carbon metabolism interact with anthocyanin biosynthesis during grape berry development: does the accumulation of anthocyanins, which represents a non-negligible diversion of carbon metabolic fluxes, require reprogramming of central enzymes or is it controlled downstream of central metabolism? To this end, 23 enzymes involved in central carbon metabolism pathways have been analyzed in the berries of 3 grape cultivars, which have close genetic background but distinct temporal dynamics of anthocyanin accumulation.

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.

Evaluation of Polarized Projective Mapping as a possible tool for attributing South African Chenin blanc dry wine styles

Multiple Factor Analysis (MFA) According to the Chenin blanc Association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded (RRU), and Rich and Ripe Wooded (RRW), classically attributed with the help of sensory evaluation. One of the “rapid methods” has drawn our attention for the purpose of simplifying and making style attribution for large sample sets, evaluated during different sessions, more robust. Polarized Projective Mapping (PPM) is a hybrid of Projective Mapping (PM) and Polarised Sensory Positioning (PSP). It is a reference-based method in which poles
(references) are used for the evaluation of similarities and dissimilarities between samples.

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Sensory definition of green aroma concept in red French wines. Evidence for the contribution of novel volatile markers

The aromatic complexity of a wine results from the perception of the association of volatile molecules and each aroma can be categorized into different families. The “green” aromas family in red wines has retained our attention by its close link with the fruity perception. In that study, the “green” olfactory concept of red wines was considered through a strategy combining both sensory analysis and hyphenated chromatographic techniques including HPLC and MDGC (Multidimensional Gas Chromatography). The aromatic space of this concept was specified by lexical generation through a free association task on 22 selected wines by a panel of wine experts. Then, 70 French red wines were scored on the basis of the intensity of their “green” and “fruity” attributes.