Macrowine 2021
IVES 9 IVES Conference Series 9 A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

Abstract

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”. Biotechnological products are now available for effective antimicrobial actions. Lysozyme inhibits the lactic acid bacteria. Chitosan also acts on the lactic acid bacteria but also efficiently on Brettanomyces. Acid sorbic can be a help to control the development of the undesirable strains yeast strains. On the antioxidant effect oak wood could potentially play a protective role. The development of an electrochemical sensor was used to estimate the influence of different cooperage factors on the antioxydant capacity(CaOx)suitable to be transmit by the wood at the wine. For red wines, during two successive vintages in two different wineries, tests compared classical post-MLF SO2 additions (5 g/hL) in classic new barrels to a treatment with a mixture of lysozyme and chitosan in CaOx optimized barrels. Microbiological monitoring has been done like chemical assays and wines tasted by expert panels. Barrels were also fitted with an innovative device allowing to measure dissolved oxygen without to have to open the barrel. In two different “Sauternes” wineries, test have been done to compare a classic mutage (between 10 and 25 g/hL of SO2) in classic barrels to a half a dose of SO2 and adding a supplement chitosan and sorbic acid in in CaOx optimized barrels. The impact on the microbial flora were investigated as well as the impact on the quality of wine. The innovative device for measuring dissolved oxygen was also used. All these wines were followed until the end of the harvest of breeding, ie on breeding periods of 6-18 months to understand the stability over time of the results. The results show that the test wines are microbiologically more stable than control wines. In the most of the case, always for the wine experiments and often for the “liqoureux”, the dissolved oxygen levels are also lower. Some metabolites (volatile phenols, diacetyl, and acetaldehyde) are less concentrated and colors are more intense and more stable. At the end, the combination of biotechnology tools and CAOX appears of a very efficient has emerged as a very effective technique to reduce the amount of total SO2 in wine as currently requested by consumers and by legislators.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Vincent Renouf*, Marie Mirabel

*Chêne & Cie

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

INTRODUCTION: Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines.

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues.

Using elicitors in different grape varieties. Effect over their phenolic composition

Phenolic compounds are very important in crop plants and have been the subject of a large number of studies. Three main reasons can be cited for optimizing the level of phenolic compounds in crop plants: their physiological role in plants, their technological significance for food processing, and their nutritional characteristics1 Indeed, an enormous diversity of phenolic antioxidants is found in fruits and vegetables, and their presence and roles can be affected or modified by several pre- and postharvest cultural practices and/or food processing technologies (Ruiz-García et al. 2012, Goldman et al. 1999, Tudela et al. 2002). In winegrapes, the technological importance of phenolic compounds, mainly flavonoids, is well-known.

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.