Macrowine 2021
IVES 9 IVES Conference Series 9 A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

Abstract

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”. Biotechnological products are now available for effective antimicrobial actions. Lysozyme inhibits the lactic acid bacteria. Chitosan also acts on the lactic acid bacteria but also efficiently on Brettanomyces. Acid sorbic can be a help to control the development of the undesirable strains yeast strains. On the antioxidant effect oak wood could potentially play a protective role. The development of an electrochemical sensor was used to estimate the influence of different cooperage factors on the antioxydant capacity(CaOx)suitable to be transmit by the wood at the wine. For red wines, during two successive vintages in two different wineries, tests compared classical post-MLF SO2 additions (5 g/hL) in classic new barrels to a treatment with a mixture of lysozyme and chitosan in CaOx optimized barrels. Microbiological monitoring has been done like chemical assays and wines tasted by expert panels. Barrels were also fitted with an innovative device allowing to measure dissolved oxygen without to have to open the barrel. In two different “Sauternes” wineries, test have been done to compare a classic mutage (between 10 and 25 g/hL of SO2) in classic barrels to a half a dose of SO2 and adding a supplement chitosan and sorbic acid in in CaOx optimized barrels. The impact on the microbial flora were investigated as well as the impact on the quality of wine. The innovative device for measuring dissolved oxygen was also used. All these wines were followed until the end of the harvest of breeding, ie on breeding periods of 6-18 months to understand the stability over time of the results. The results show that the test wines are microbiologically more stable than control wines. In the most of the case, always for the wine experiments and often for the “liqoureux”, the dissolved oxygen levels are also lower. Some metabolites (volatile phenols, diacetyl, and acetaldehyde) are less concentrated and colors are more intense and more stable. At the end, the combination of biotechnology tools and CAOX appears of a very efficient has emerged as a very effective technique to reduce the amount of total SO2 in wine as currently requested by consumers and by legislators.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Vincent Renouf*, Marie Mirabel

*Chêne & Cie

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

Defining the mechanisms and impact of winemaking treatments on tannin and polysaccharides in red wine: recent progress in creating diverse styles

Tannin and polysaccharide concentration and composition is important in defining the texture of red wines, but can vary due to factors such as cultivar, region, grape ripeness, viticultural practices and winemaking techniques. However, the concentration and composition of these macromolecules is dependent not only on grape tannin and polysaccharide concentration and composition, but also their extractability and, in the case of polysaccharides, their formation by yeast. Through studies into the influence of grape maturity, winemaking and sensory impacts of red grape polysaccharides, seed and skin tannins, recent research in our laboratory has shown that the processes involved in the extraction of these macromolecules from grapes and their retention in wine are very complex.

The impact of different yeasts and harvest time on the wine quality of Beihong and Beimei (<I>V. vinifera x V. amurensis</I>)

Beihong and Beimei are two wine cultivars from ‘Muscat Hamberg’ (V. vinifera L.) and wild V. amurensis Rupr., which were released in China in 2008. Here,two enology practices were reported. Firstly, the impact of different yeasts including D254, GRE, K1, D21 and BDX on dry wine quality of Beihong and Beimei was investigated. For Beihong, among wines fermented by all yeasts, residual sugar content was the lowest, total anthocyanin and resveratrol contents were the highest in the wine by D254. However, the wine by D254 had lower titrable acid than those by the other yeasts except BDX.

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.

Field-grown Sauvignon Blanc berries react to increased exposure by controlling antioxidant homeostasis and displaying UV acclimation responses that are influenced by the level of ambient light

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).