Macrowine 2021
IVES 9 IVES Conference Series 9 A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

Abstract

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”. Biotechnological products are now available for effective antimicrobial actions. Lysozyme inhibits the lactic acid bacteria. Chitosan also acts on the lactic acid bacteria but also efficiently on Brettanomyces. Acid sorbic can be a help to control the development of the undesirable strains yeast strains. On the antioxidant effect oak wood could potentially play a protective role. The development of an electrochemical sensor was used to estimate the influence of different cooperage factors on the antioxydant capacity(CaOx)suitable to be transmit by the wood at the wine. For red wines, during two successive vintages in two different wineries, tests compared classical post-MLF SO2 additions (5 g/hL) in classic new barrels to a treatment with a mixture of lysozyme and chitosan in CaOx optimized barrels. Microbiological monitoring has been done like chemical assays and wines tasted by expert panels. Barrels were also fitted with an innovative device allowing to measure dissolved oxygen without to have to open the barrel. In two different “Sauternes” wineries, test have been done to compare a classic mutage (between 10 and 25 g/hL of SO2) in classic barrels to a half a dose of SO2 and adding a supplement chitosan and sorbic acid in in CaOx optimized barrels. The impact on the microbial flora were investigated as well as the impact on the quality of wine. The innovative device for measuring dissolved oxygen was also used. All these wines were followed until the end of the harvest of breeding, ie on breeding periods of 6-18 months to understand the stability over time of the results. The results show that the test wines are microbiologically more stable than control wines. In the most of the case, always for the wine experiments and often for the “liqoureux”, the dissolved oxygen levels are also lower. Some metabolites (volatile phenols, diacetyl, and acetaldehyde) are less concentrated and colors are more intense and more stable. At the end, the combination of biotechnology tools and CAOX appears of a very efficient has emerged as a very effective technique to reduce the amount of total SO2 in wine as currently requested by consumers and by legislators.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Vincent Renouf*, Marie Mirabel

*Chêne & Cie

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.

Defining the mechanisms and impact of winemaking treatments on tannin and polysaccharides in red wine: recent progress in creating diverse styles

Tannin and polysaccharide concentration and composition is important in defining the texture of red wines, but can vary due to factors such as cultivar, region, grape ripeness, viticultural practices and winemaking techniques. However, the concentration and composition of these macromolecules is dependent not only on grape tannin and polysaccharide concentration and composition, but also their extractability and, in the case of polysaccharides, their formation by yeast. Through studies into the influence of grape maturity, winemaking and sensory impacts of red grape polysaccharides, seed and skin tannins, recent research in our laboratory has shown that the processes involved in the extraction of these macromolecules from grapes and their retention in wine are very complex.

Application of high power ultrasounds during red wine vinification

Wine color is one of the main organoleptic characteristics influencing its quality. It is of especial interest in red vinifications due to the economic resources that wineries have to invest for the extraction of the phenolic compounds responsible of wine color, compounds that are mainly located inside the skin cell vacuoles. Moreover, these phenolic compounds not only influence color but also other organoleptic properties such as body, mouthfeel, astringency and flavour. The transference of phenolic compounds from grapes to must during vinification is closely related with the type of grapes and the winemaking technique.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze.