Macrowine 2021
IVES 9 IVES Conference Series 9 Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Abstract

Laboratório de Análisis del Aroma y Enologia (LAAE). Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, 50009, Zaragoza, Spain, During alcoholic fermentation, fusel (or Strecker) aldehydes are intermediates in the amino acid catabolism to form fusel alcohols following the Ehrlich Pathway (1). One of the main enzymes involved in this pathway is Alcohol Dehydrogenase (ADH), whose activity is highly strain dependent and determines the rate of conversion of aldehydes into fusel alcohols (2). This enzyme has a Zn2+ catalytic binding site, which suggests that the must Zn2+ levels will most likely influence the rate of reduction of aldehydes into alcohols. On the other hand, SO2 is commonly used in winemaking for its antiseptic and antioxidant properties. This molecule is highly reactive and can form strong associations (alkylhydroxy sulfonates) with aldehydes. Levels of SO2 present in the alcoholic fermentation could then, at least theoretically, prevent the reduction of aldehydes to alcohols. Aldehydes could accumulate under the form of non-volatile adducts which could be released back once SO2 levels drop with aging or oxidation contributing to the development of oxidation off-odors (3). A set-up of fermentations of synthetic must containing known content of amino acids and nitrogen sources as well as elements necessary to the yeast metabolism were prepared. Different levels of Zinc or SO2 were tested. Three different commercial Saccharomyces cereviase yeast strains were selected for fermentation. Fusel alcohols and Strecker aldehydes were determined in the finished wines by GC-FID and GC-MS, respectively. Results confirm that all the factors are significant and that must Zn and SO2 levels influence the presence of Strecker aldehydes in the final wine.

1. Hazelwood, L. A.; Daran, J. M.; van Maris, A. J.; Pronk, J. T.; Dickinson, J. R., The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism. Appl Environ Microbiol 2008, 74, 2259-66. 2. Singh, R.; Kunkee, R. E., Alcohol Dehydrogenase Activities of Wine Yeasts in Relation to Higher Alcohol Formation. Applied and Environmental Microbiology 1976, 32, 666 – 670. 3. Bueno, M.; Franco-Luesma, E.; Carrascon, V.; Ferreira, V., Evaluation of key and bound aroma carbonyls in wine for a better understanding of their release or formation through oxidation. Flavour Science. Proceedings of the XIV Weurman Flavour Research Symposium 2015, 397-402.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Inês Oliveira*, Mónica Bueno, Purificación Hernández-Orte, Vicente Ferreira

*University of Zaragoza

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Ageing of sweet wines: oxygen evolution according to bung and barrel type

Barrel ageing is a crucial step in the wine process because it allows many changes to the wine as enrichment, colour stabilization, clarification and also a slow oxygenation. Effects of the oak barrel have to be known to prevent oxidation of the wine. The type of bung used during ageing is also a parameter to consider. Ageing sweet wines in barrel is a real challenge. These wines may need some oxygen at the beginning of ageing but they should be protected at the end of their maturation, to avoid oxidation.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Correlations between sensory characteristics and colloidal content in dry white wines

Must clarification is an important step occurring just after grape extraction in the elaboration of white wine, consisting in a solid-liquid separation. Traditionally, low must turbidity, around 50-150 NTU, is generally reached in white winemaking in order to prevent reductive aromas and facilitating alcoholic fermentation. Alternatively, a higher turbidity (300 NTU or above) can be sought for reasons such as a better expression of grapes identity (terroir), or for getting a must matrix that could supposedly lead to wines having greater ageing potential.

Innovations in the use of bentonite in enology: interactions with grape and wine proteins, colloids, polyphenols and aroma compounds.

The use of bentonite in oenology rounds around the limpidity and the stability that determine consumer acceptability. As a matter of fact, the haze formation in wine reduces its commercial value and makes it unacceptable for sale. Stabilization treatments are, therefore, essential to ensure a long-time limpidity and to forecast the formation of deposits in the bottle. Bentonite that is normally used in oenology for clarifying-fining purpose, shows a natural clay-based mineral structure allowing it to swell and to jelly in water and hence in must and wine.