Macrowine 2021
IVES 9 IVES Conference Series 9 Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Abstract

Laboratório de Análisis del Aroma y Enologia (LAAE). Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, 50009, Zaragoza, Spain, During alcoholic fermentation, fusel (or Strecker) aldehydes are intermediates in the amino acid catabolism to form fusel alcohols following the Ehrlich Pathway (1). One of the main enzymes involved in this pathway is Alcohol Dehydrogenase (ADH), whose activity is highly strain dependent and determines the rate of conversion of aldehydes into fusel alcohols (2). This enzyme has a Zn2+ catalytic binding site, which suggests that the must Zn2+ levels will most likely influence the rate of reduction of aldehydes into alcohols. On the other hand, SO2 is commonly used in winemaking for its antiseptic and antioxidant properties. This molecule is highly reactive and can form strong associations (alkylhydroxy sulfonates) with aldehydes. Levels of SO2 present in the alcoholic fermentation could then, at least theoretically, prevent the reduction of aldehydes to alcohols. Aldehydes could accumulate under the form of non-volatile adducts which could be released back once SO2 levels drop with aging or oxidation contributing to the development of oxidation off-odors (3). A set-up of fermentations of synthetic must containing known content of amino acids and nitrogen sources as well as elements necessary to the yeast metabolism were prepared. Different levels of Zinc or SO2 were tested. Three different commercial Saccharomyces cereviase yeast strains were selected for fermentation. Fusel alcohols and Strecker aldehydes were determined in the finished wines by GC-FID and GC-MS, respectively. Results confirm that all the factors are significant and that must Zn and SO2 levels influence the presence of Strecker aldehydes in the final wine.

1. Hazelwood, L. A.; Daran, J. M.; van Maris, A. J.; Pronk, J. T.; Dickinson, J. R., The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism. Appl Environ Microbiol 2008, 74, 2259-66. 2. Singh, R.; Kunkee, R. E., Alcohol Dehydrogenase Activities of Wine Yeasts in Relation to Higher Alcohol Formation. Applied and Environmental Microbiology 1976, 32, 666 – 670. 3. Bueno, M.; Franco-Luesma, E.; Carrascon, V.; Ferreira, V., Evaluation of key and bound aroma carbonyls in wine for a better understanding of their release or formation through oxidation. Flavour Science. Proceedings of the XIV Weurman Flavour Research Symposium 2015, 397-402.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Inês Oliveira*, Mónica Bueno, Purificación Hernández-Orte, Vicente Ferreira

*University of Zaragoza

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Condensed tannin is a principle group of polyphenol compounds derived from grape, greatly contributing to the bioactivity and the sensory perception of wine. Condensed tannins present as a heterogeneous mixture in nature involving various degrees of both polymerization and galloylation. Even though multiple attempts focusing on fractionation of grape condensed tannins by solid-phase have been conducted over the past decades, few individual tannins have been purified and identified. Hence, our knowledge on grape and wine condensed tannin moleculars has to be limited at the several known monomeric, dimeric and trimeric proanthocyanidins

Sensory impacts of the obturator used for the Chasselas: study over the time

Many parameters affect the organoleptic characteristics of wine: internal parameters like the chemical composition or polyphenol content and external as for example storage conditions or the type of obturator. The aim of this study was to characterize sensorally the impacts of several type of obturator on a white wine: Chasselas. To determine the organoleptic characteristics of this wine, a quantitative descriptive analysis could be used. But rapid sensory methods were preferred in this project. Indeed these methods are an appropriate alternative to conventional descriptive methods for quickly assessing sensory product discrimination.

The commercial yeast strain as a significant source of variance for tyrosol and hydroxytyrosol in white wine

Tyrosol (TYR) and hydroxytyrosol (HYT) are bioactive phenols present in olive oil and wine, basic elements of the Mediterranean diet. TYR is reported in the literature for its interesting antioxidant, cardioprotective and anti-inflammatory properties. In wine, its concentration can reach values as high as about 40 mg/L
[Pour Nikfardjam et al. 2007] but, more frequently, this phenol – derived from yeast metabolism of tyrosine during fermentation – is present at lower levels, generally higher in red wines compared to whites. HYT was measured for the first time by Di Tommaso et al. [1998] in Italian wines – with maximum values of 4.20 mg/L and 1.92 mg/L for red and white wines, respectively – while definitely lower concentrations have been found later in Greek samples.

Proteomic and activity characterization of exocellular laccases from three Botrytis cinerea strains

Botrytis cinerea is a fungus that causes common infection in grapes and other fruits. In winemaking, its presence can be both considered desirable in the case of noble rot infection or undesirable when grey rot is developed. This fungus produces an extracellular enzyme known as laccase which is able to cause oxidation of phenolic compounds present in must and wine, causing most of the times a decrease in its quality and problems during the winemaking process [1]. Material and methods: Three B. cinerea strains (B0510, VA612 and RM344) were selected and grown in a liquid medium adapted from one previously described [2]. The enzyme was isolated by tangential ultrafiltration of the culture medium using a QuixStand system equipped with a 30 KDa filtration membrane.