Macrowine 2021
IVES 9 IVES Conference Series 9 Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Influence of SO2 and Zinc on the formation of volatile aldehydes during alcoholic fermentation

Abstract

Laboratório de Análisis del Aroma y Enologia (LAAE). Department of Analytical Chemistry, Faculty of Sciences, Universidad de Zaragoza, 50009, Zaragoza, Spain, During alcoholic fermentation, fusel (or Strecker) aldehydes are intermediates in the amino acid catabolism to form fusel alcohols following the Ehrlich Pathway (1). One of the main enzymes involved in this pathway is Alcohol Dehydrogenase (ADH), whose activity is highly strain dependent and determines the rate of conversion of aldehydes into fusel alcohols (2). This enzyme has a Zn2+ catalytic binding site, which suggests that the must Zn2+ levels will most likely influence the rate of reduction of aldehydes into alcohols. On the other hand, SO2 is commonly used in winemaking for its antiseptic and antioxidant properties. This molecule is highly reactive and can form strong associations (alkylhydroxy sulfonates) with aldehydes. Levels of SO2 present in the alcoholic fermentation could then, at least theoretically, prevent the reduction of aldehydes to alcohols. Aldehydes could accumulate under the form of non-volatile adducts which could be released back once SO2 levels drop with aging or oxidation contributing to the development of oxidation off-odors (3). A set-up of fermentations of synthetic must containing known content of amino acids and nitrogen sources as well as elements necessary to the yeast metabolism were prepared. Different levels of Zinc or SO2 were tested. Three different commercial Saccharomyces cereviase yeast strains were selected for fermentation. Fusel alcohols and Strecker aldehydes were determined in the finished wines by GC-FID and GC-MS, respectively. Results confirm that all the factors are significant and that must Zn and SO2 levels influence the presence of Strecker aldehydes in the final wine.

1. Hazelwood, L. A.; Daran, J. M.; van Maris, A. J.; Pronk, J. T.; Dickinson, J. R., The Ehrlich pathway for fusel alcohol production: a century of research on Saccharomyces cerevisiae metabolism. Appl Environ Microbiol 2008, 74, 2259-66. 2. Singh, R.; Kunkee, R. E., Alcohol Dehydrogenase Activities of Wine Yeasts in Relation to Higher Alcohol Formation. Applied and Environmental Microbiology 1976, 32, 666 – 670. 3. Bueno, M.; Franco-Luesma, E.; Carrascon, V.; Ferreira, V., Evaluation of key and bound aroma carbonyls in wine for a better understanding of their release or formation through oxidation. Flavour Science. Proceedings of the XIV Weurman Flavour Research Symposium 2015, 397-402.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Inês Oliveira*, Mónica Bueno, Purificación Hernández-Orte, Vicente Ferreira

*University of Zaragoza

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T

Wood from barrique: release of phenolic compounds and permeability to oxygen

Chemical and sensory changes occurring in red wine during ageing in oak barrique are due to the slow and gradual entrance of oxygen along with a release of ellagic tannin from the wood. Though oxygen can enter the cask through the bunghole, it is not clear the role of permeation through the wood staves as well as the amount of oxygen entering by permeation. The distribution of the released ellagic tannins in the wine ageing is also unknown. The oxygen passing through the bunghole may have a different wine ageing effect compared to the oxygen permeating through the wooden staves owing to the uneven ellagic tannin concentration throughout the wine.

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

Effects of post-fermentative cold maceration on chemical and sensory characteristics of Syrah, Cabernet Franc and Montepulciano wines

Astringency sensation decreases slowly during the aging of red wine. Complex reactions of condensation and precipitation of wine polyphenols are involved in this phenomenon. Wine composition and conditions of aging, such as temperature and oxygen availability, strongly influence evolution of the phenol matrix. Recently, a Post-Fermentative cold Maceration (PFM) technique was tested with the aim of accelerating reactions leading to the reduction of astringency and exploiting chemical compounds not extracted from the solid parts of grapes during the previous traditional maceration phase. To this purpose, an innovative maceration system was engineered and used to perform PFM trials on marc derived from vinification of different varieties of red grapes.

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.