GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Impact of grapevine leafroll virus infections on vine physiology and the berry transcriptome

Impact of grapevine leafroll virus infections on vine physiology and the berry transcriptome

Abstract

Context and purpose of the study ‐ Grapevine leafroll associated virus (GLRaV) infections deteriorate vine physiological performance and cause high losses of yield and fruit quality and are thus causing serious economic losses in the wine industry. Physiological and molecular studies on the impact of leafroll infections on plant and fruit metabolism are relatively scare and the molecular mechanisms associated with the plant response to the virus during berry ripening are not well understood so far. Commonly observed phenotypic alterations consist in a ripening delay, a reduction in anthocyanin and sugar accumulation. The few molecular studies associated in particular the lack of anthocyanin of berries of infected vines to a repression of key genes of anthocyanin biosynthesis. However such studies did never account for berry heterogeneity and potential phenological shifts induced by virus infection, which could have introduced biases in gene expression studies.

Material and methods ‐ In the present study a long‐term experiment was established in the year 2000, with the aim to investigate the effects of infections with different GLRaVs (GLRaV 1 & GLRaV 1+3) on vine and grape physiology. Physiological data (yield, vigor, photosynthesis, berry quality) has been collected from 2015 to 2018. In 2018 a transcriptomic (RNA‐seq) analysis of 2 reconstituted berry ripening stages was performed. Therefore 245 berries were individually sampled and individually analyzed for sugar and organic acids in order to re‐constitute 2 homogenous ripening stages to circumvent intercluster berry heterogeneity and thus to compensate for phenological shifts induced by virus infections. RNA of reconstituted samples was extracted and sequenced by single end sequencing and subsequently analyzed for differentially expressed genes (DEGs).

Results ‐ Physiological measurement showed a significant decrease in photosynthesis, yield and sugar content, which were highly significant in the co‐infected vines (GLRaV 1+3). RNA‐sequencing of berries revealed a total of 2136 DEGs between control and virus infections. Several transcriptions factors related to abiotic and biotic stress could be identified and showed interesting variation in dependence to ripening stage and infection severity. Strikingly previously reported repression of the anthocyanin biosynthesis and sugar metabolism could not be confirmed by gene expression. This illustrates that the main damaging effect on GLRaV infection is rather related to a phenological shift than to a direct impact on metabolism. The here reported results give new insight in the mechanism of leafroll infection and emphasize the importance of the sampling protocol of molecular studies investigating berry metabolism. 

DOI:

Publication date: June 22, 2020

Issue: GiESCO 2019

Type: Article

Authors

Markus RIENTH (1), Sana GHAFFARI (1,2), Jean‐Sébastien REYNARD (3)

(1) Changins, haute ecole de viticulture et œnologie, route de Duillier 50, 1260 Nyon, Switzerland
(2) Higher Institute of Applied Biology of Medenine, Route el Djorf km 22.5, 4119 Medenine, Tunisia
(3) Agroscope, route de Duillier 50, 1260 Nyon, Switzerland

Contact the author

Keywords

Grapevine leafroll virus, GLRaV 1, GLRaV 3, RNA‐seq, berry metabolism

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Critical investigation on additions to improve the sensory characteristics of dealcoholized wine

The demand for dealcoholized wine has been progressively increasing in recent years. Moreover, the attention for such products is probably increasing even more. Due to that increasing demand and market awareness the legal authorities are about changing rules for that products. Also, at OIV level, these products are being intensively discussed for certain time. The production of dealcoholized wine bases on wine as initial product. This wine is then reduced by physical methods to an alcohol content of less than 0.5% vol., or in other words, to less than 4g/l of alcohol. There are various technologies are possible for producing dealcoholized wine (Schmitt and Christmann 2019).

Grapevine yield-gap: identification of environmental limitations by soil and climate zoning in Languedoc-Roussillon region (south of France)

Grapevine yield has been historically overlooked, assuming a strong trade-off between grape yield and wine quality. At present, menaced by climate change, many vineyards in Southern France are far from the quality label threshold, becoming grapevine yield-gaps a major subject of concern. Although yield-gaps are well studied in arable crops, we know very little about grapevine yield-gaps. In the present study, we analysed the environmental component of grapevine yield-gaps linked to climate and soil resources in the Languedoc Roussillon. We used SAFRAN data and IGP Pays d’Oc wine yields from 2010 to 2018. We selected climate and soil indicators proving to have a significant effect on average wine yield-gaps at the municipality scale. The most significant factors of grapevine yield were the Soil Available Water Capacity; followed by the Huglin Index and the Climatic Dryness Index. The Days of Frost; the Soil pH; and the Very Hot Days were also significant. Then, we clustered geographical zones presenting similar indicators, facilitating the identification of resources yield-gaps. We discussed the number of zones with the experts of IGP Pays d’Oc label, obtaining 7 zones with similar limitations for grapevine yield. Finally, we analysed the main resources causing yield-gaps and the grapevine varieties planted on each zone. Mapping grapevine resource yield-gaps are the first stage for understanding grapevine yield-gaps at the regional scale.

Techniques for sunburn reduction in bunches in Vitis vinifera L. cv. Graciano

Sunburn results from a combination of excessive photosynthetically active radiation (PAR) and UV radiation and temperature that can be exacerbated by other stress factors such as water deficit. Sunburn is a physiological disorder that affects the visual and organoleptic properties of grapes. The appearance of brown and necrotic spots severely affects the commercial value of the fruit, and in extreme cases, significantly decreases yield. This damage occurs with some frequency in sensitive varieties such as Graciano.

Exploring multisensory interactions through the study of astringency diversity of mono-varietal Italian red wines

According to the OIV Focus 2017 estimating the vine varieties distribution in the world, Italy is the richest grape producing country in terms of varieties.

Assessing the Effectiveness of Electrodialysis in Controlling Brettanomyces Growth in Wine

Brettanomyces yeast can negatively impact the quality and stability of wines, posing a significant challenge to winemakers. [1] This study aims to develop novel management practices to limit Brettanomyces impact on wines by evaluating the effectiveness of electrodialysis (ED) technology in removing magnesium (Mg2+) from wine to prevent the development of Brettanomyces yeast. The ED technique utilizes charged membranes to extract ions from the wine, and it is considered an alternative to cold stabilization that requires less energy. [2]