GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Impact of grapevine leafroll virus infections on vine physiology and the berry transcriptome

Impact of grapevine leafroll virus infections on vine physiology and the berry transcriptome

Abstract

Context and purpose of the study ‐ Grapevine leafroll associated virus (GLRaV) infections deteriorate vine physiological performance and cause high losses of yield and fruit quality and are thus causing serious economic losses in the wine industry. Physiological and molecular studies on the impact of leafroll infections on plant and fruit metabolism are relatively scare and the molecular mechanisms associated with the plant response to the virus during berry ripening are not well understood so far. Commonly observed phenotypic alterations consist in a ripening delay, a reduction in anthocyanin and sugar accumulation. The few molecular studies associated in particular the lack of anthocyanin of berries of infected vines to a repression of key genes of anthocyanin biosynthesis. However such studies did never account for berry heterogeneity and potential phenological shifts induced by virus infection, which could have introduced biases in gene expression studies.

Material and methods ‐ In the present study a long‐term experiment was established in the year 2000, with the aim to investigate the effects of infections with different GLRaVs (GLRaV 1 & GLRaV 1+3) on vine and grape physiology. Physiological data (yield, vigor, photosynthesis, berry quality) has been collected from 2015 to 2018. In 2018 a transcriptomic (RNA‐seq) analysis of 2 reconstituted berry ripening stages was performed. Therefore 245 berries were individually sampled and individually analyzed for sugar and organic acids in order to re‐constitute 2 homogenous ripening stages to circumvent intercluster berry heterogeneity and thus to compensate for phenological shifts induced by virus infections. RNA of reconstituted samples was extracted and sequenced by single end sequencing and subsequently analyzed for differentially expressed genes (DEGs).

Results ‐ Physiological measurement showed a significant decrease in photosynthesis, yield and sugar content, which were highly significant in the co‐infected vines (GLRaV 1+3). RNA‐sequencing of berries revealed a total of 2136 DEGs between control and virus infections. Several transcriptions factors related to abiotic and biotic stress could be identified and showed interesting variation in dependence to ripening stage and infection severity. Strikingly previously reported repression of the anthocyanin biosynthesis and sugar metabolism could not be confirmed by gene expression. This illustrates that the main damaging effect on GLRaV infection is rather related to a phenological shift than to a direct impact on metabolism. The here reported results give new insight in the mechanism of leafroll infection and emphasize the importance of the sampling protocol of molecular studies investigating berry metabolism. 

DOI:

Publication date: June 22, 2020

Issue: GiESCO 2019

Type: Article

Authors

Markus RIENTH (1), Sana GHAFFARI (1,2), Jean‐Sébastien REYNARD (3)

(1) Changins, haute ecole de viticulture et œnologie, route de Duillier 50, 1260 Nyon, Switzerland
(2) Higher Institute of Applied Biology of Medenine, Route el Djorf km 22.5, 4119 Medenine, Tunisia
(3) Agroscope, route de Duillier 50, 1260 Nyon, Switzerland

Contact the author

Keywords

Grapevine leafroll virus, GLRaV 1, GLRaV 3, RNA‐seq, berry metabolism

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Climate change projections to support the transition to climate-smart viticulture

The Earth’s system is undergoing major changes through a wide range of spatial and temporal scales as a response to growing anthropogenic radiative forcing, which is pushing the whole system far beyond its natural variability. Sources of greenhouse gases largely exceed their sinks, thus leading to a strengthened greenhouse effect. More energy is thereby being supplied to the system, with inevitable shifts in climatic patterns and weather regimes. Over the last decades, these modifications have been manifested in the full statistical distributions of the atmospheric variables, with dramatic changes in the frequency and intensity of extremes. Natural hazards, such as severe droughts, floods, forest fires, or heatwaves, are being triggered by extreme atmospheric events worldwide, thus threatening human activities. Viticultculture is not only exposed to changing climates but is also highly vulnerable, as grapevine phenology and physiological development are strongly controlled by atmospheric conditions. Therefore, the assessment of climate change projections for a given region is critical for climate change adaptation and risk reduction in viticulture. By adopting timely and suitable measures, the future sustainability and resiliency of the sector can be fostered. Climate-grapevine chain modelling is an essential tool for better planning and management. However, the accuracy of the resulting projections is limited by many uncertainties that must be duly taken into account when transferring knowledge to stakeholders and decision-makers. Climate-smart viticulture will comprise ensembles of locally tuned strategies, envisioning both adaptation and mitigation, assisted by emerging technologies and decision-support systems.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Grapevine sugar concentration model in the Douro Superior, Portugal

Increasingly warm and dry climate conditions are challenging the viticulture and winemaking sector. Digital technologies and crop modelling bear the promise to provide practical answers to those challenges. As viticultural activities strongly depend on harvest date, its early prediction is particularly important, since the success of winemaking practices largely depends upon this key event, which should be based on an accurate and advanced plan of the annual cycle. Herein, we demonstrate the creation of modelling tools to assess grape ripeness, through sugar concentration monitoring. The study area, the Portuguese Côa valley wine region, represents an important terroir in the “Douro Superior” subregion. Two varieties (cv. Touriga Nacional and Touriga Franca) grown in five locations across the Côa Region were considered. Sugar accumulation in grapes, with concentrations between 170 and 230 g l-1, was used from 2014 to 2020 as an indicator of technological maturity conditioned by meteorological factors. The climatic time series were retrieved from the EU Copernicus Service, while sugar data were collected by a non-profit organization, ADVID, and by Sogrape, a leading wine company. The software for calibrating and validating this model framework was the Phenology Modeling Platform (PMP), version 5.5, using Sigmoid and growing degree-day (GDD) models for predictions. The performance was assessed through two metrics: Roots Mean Square Error (RMSE) and efficiency coefficient (EFF), while validation was undertaken using leave-one-out cross-validation. Our findings demonstrate that sugar content is mainly dependent on temperature and air humidity. The models achieved a performance of 0.65

Investigating the impact of grape exposure and UV radiations on rotundone in Vitis vinifera L. Tardif grapes under field trial conditions

Rotundone is the main aroma compound responsible for peppery notes in wines whose biosynthesis is negatively affected by heat and drought. Through the alteration of precipitation regime and the increase in temperature during maturation, climate change is expected to affect wine peppery typicality. In this context there is a demand for developing sustainable viticultural strategies to enhance rotundone accumulation or limit its degradation. It was recently proposed that ultraviolet (UV) radiations could stimulate rotundone production. The aim of this study was to investigate under field trial conditions the impact of grape exposure and UV treatments on rotundone in Vitis vinifera L. Tardif, an almost extinct grape variety from south-west France that can express particularly high rotundone levels. Four different treatments were compared in 2021 to a control treatment using a randomised complete block design with three replications per treatment. Grape exposure was manipulated through early or late defoliation. Leaf and laterals shoots were removed at Eichorn Lorenz growth stages 32 or 34 on the morning-sun side of the canopy. During grape maturation, UV radiations were either reduced by 99% by installing UV radiation-shielding sheets, or applied four times using the Boxilumix™ non thermal device (Asclepios Tech, Tournefeuille) with the aim of activating plant signalling pathway. Loggers displayed in solar radiation shields were used to assess the effect of such shielding sheets on air temperature within the bunch zone. The composition of grapes subjected to these treatments will be soon analysed for their rotundone content and basic classical laboratory analyses. Grapes will be harvested to elaborate wines under standardized small-scale vinification conditions (60kg) that will be assessed by a trained sensory panel.