GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Early detection project – make a GTD infection visible without disease symptoms

Early detection project – make a GTD infection visible without disease symptoms

Abstract

Context and purpose of the study ‐ The presence of grapevine trunk diseases (GTDs) related pathogens leads to severe economic losses in wine‐growing regions all over the world. GTDs cause foliar discoloration, stunted growth, decline, sectorial and/or central necrosis of the trunk wood, and dieback, while the quality and the quantity of the grapes and therefore the wine production is reduced. The disease management is challenging for vine‐growers since the responsible fungi colonize wood tissues (and are therefore inaccessible for conventional fungicides) and the related symptoms occur mostly after a long period of latency. The aims of this project were first to distinguish between healthy and infected plants before the symptoms appear and second to document the efficacy of BASF ´s Tessior®‐ System for wound protection under field conditions.

Material and methods ‐ Long term field trials were established between 2014 and 2015 in Germany, France, Greece, and Italy, where each year the pruning wounds are treated with Tessior®. In order to increase the infection pressure, some of the vineyards are artificially inoculated with spores of Phaeomoniellachlamydospora and Botryosphaeriaceae species. The presence of P. chlamydospora – a pathogen causing esca‐disease – and Botryosphaeriaceae species – causing Botryosphaeria dieback – in grapevines was determined with an optimized protocol. Samples were collected by drilling a 5 mm diameter hole in the spurs below a pruning wound which was closed then with a wound sealant. The wood chips were lyophilized and afterwards homogenized using TissueLyser II (Qiagen). Total genomic DNA was extracted from the grapevine samples and quantitative Real‐Time PCR using TaqMan probes was performed.

Results ‐ This protocol has been proved to be fast and accurate to quantify the DNA amount of GTDs related pathogens in grapevine wood. Furthermore, the efficacy of Tessior® wound protectant has been verified showing significant reduction of infection with P. chlamydospora and Botryosphaeriaceae species.

DOI:

Publication date: June 22, 2020

Issue: GiESCO 2019

Type: Article

Authors

Szabina LENGYEL (1), Randall E. GOLD (2), Jochen FISCHER (1), Alexander YEMELIN (1), Eckhard THINES (1), Annett KÜHN (2)

(1) Institut für Biotechnologie und Wirkstoff-Forschung gGmbH, Erwin-Schrödinger-Straße 56, D-67663 Kaiserslautern, Germany
(2) BASF SE, Agricultural Center, Speyerer Straße 2, D-67117 Limburgerhof, Germany

Contact the author

Keywords

Grapevine, Phaeomoniellachlamydospora, Botryosphaeriaceae, quantitative Real‐Time PCR, TaqMan, Tessior®

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Utilizing ozone for the management of powdery mildew (Erysiphe necator Schwein.) in vineyards: potential and challenges

Powdery mildew, caused by fungal pathogens, poses a significant threat to grapevines in the DOCa Rioja region. In efforts to improve control strategies while reducing reliance on conventional phytosanitary products, ozone could constitute a potential alternative. However, it has short persistence, thus requiring frequent treatments. This study aimed to assess the suitability of ozone as an active substance for controlling powdery mildew within a phytosanitary strategy aimed at reducing conventional phytosanitary product usage. The strategy integrating ozone with conventional products yielded powdery mildew levels comparable to conventional treatments in both disease incidence and severity.

An exploration of South Tyrolean Pinot blanc wines and their quality potential in vineyard sites across a range of altitudes

Aim: Pinot Blanc is the third most planted white wine grape in northern Italy’s region of South Tyrol, where small-scale viticultural production permits the examination of the wine’s diverse expressive potential in a small area across a wide range of climatic variables. This study aimed to explore the qualitative potential of Pinot Blanc across a range of climatic variation leading to site-specific terroir expression in a cool climate region.

Différenciation mésoclimatique des terroirs alsaciens et relation avec les paramètres du milieu naturel

The influence of climatic conditions on the development of the vine and on the quality of the wines no longer needs to be demonstrated at the scale of the vineyard, by the regional climatic characteristics, determining on this scale the viticultural potentialities (Huglin, 1978; Branas, 1946; Riou et al ., 1994); but also on a local scale, at the level of the basic terroir unit (Morlat, 1989), by the landscape differentiation of the natural environment inducing climatic variability within the same vineyard, and partly explaining differences in functioning of the vine, in connection with the processes of maturation and the quality of the wine (Becker, 1977 and 1984; Morlat, 1989 and Lebon, 1993a). According to these authors, the climatic diversity in a wine region constitutes in addition to the edaphic component, an important component of characterization of the Basic Terroir Units (UTB).

Oenological potential of cv. Tortojona: A minority grape variety from Extremadura, southwest Spain

This work, included in the VAVEGEX project, aims to evaluate the oenological, phenolic, chromatic and sensory characteristics of the grapes, must and wines produced from cv. Tortojona, minority variety grown in Extremadura region (Southwest, Spain).

Assessment of antimicrobial effect of chitosan extracted from different sources against unwanted wine microorganisms

During wine production process high attention to the microbiological control from fermentation of the grape must to bottling is necessary. In fact, control of the indigenous microflora of the grape ensures correct fermentation activity of the inoculated starter, while control of the microorganisms in the finished wine is essential to prevent wine spoilage and to ensure the dominance of the desired bacteria when malolactic fermentation is required (Mas and Portillo, 2022).