GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Early detection project – make a GTD infection visible without disease symptoms

Early detection project – make a GTD infection visible without disease symptoms

Abstract

Context and purpose of the study ‐ The presence of grapevine trunk diseases (GTDs) related pathogens leads to severe economic losses in wine‐growing regions all over the world. GTDs cause foliar discoloration, stunted growth, decline, sectorial and/or central necrosis of the trunk wood, and dieback, while the quality and the quantity of the grapes and therefore the wine production is reduced. The disease management is challenging for vine‐growers since the responsible fungi colonize wood tissues (and are therefore inaccessible for conventional fungicides) and the related symptoms occur mostly after a long period of latency. The aims of this project were first to distinguish between healthy and infected plants before the symptoms appear and second to document the efficacy of BASF ´s Tessior®‐ System for wound protection under field conditions.

Material and methods ‐ Long term field trials were established between 2014 and 2015 in Germany, France, Greece, and Italy, where each year the pruning wounds are treated with Tessior®. In order to increase the infection pressure, some of the vineyards are artificially inoculated with spores of Phaeomoniellachlamydospora and Botryosphaeriaceae species. The presence of P. chlamydospora – a pathogen causing esca‐disease – and Botryosphaeriaceae species – causing Botryosphaeria dieback – in grapevines was determined with an optimized protocol. Samples were collected by drilling a 5 mm diameter hole in the spurs below a pruning wound which was closed then with a wound sealant. The wood chips were lyophilized and afterwards homogenized using TissueLyser II (Qiagen). Total genomic DNA was extracted from the grapevine samples and quantitative Real‐Time PCR using TaqMan probes was performed.

Results ‐ This protocol has been proved to be fast and accurate to quantify the DNA amount of GTDs related pathogens in grapevine wood. Furthermore, the efficacy of Tessior® wound protectant has been verified showing significant reduction of infection with P. chlamydospora and Botryosphaeriaceae species.

DOI:

Publication date: June 22, 2020

Issue: GiESCO 2019

Type: Article

Authors

Szabina LENGYEL (1), Randall E. GOLD (2), Jochen FISCHER (1), Alexander YEMELIN (1), Eckhard THINES (1), Annett KÜHN (2)

(1) Institut für Biotechnologie und Wirkstoff-Forschung gGmbH, Erwin-Schrödinger-Straße 56, D-67663 Kaiserslautern, Germany
(2) BASF SE, Agricultural Center, Speyerer Straße 2, D-67117 Limburgerhof, Germany

Contact the author

Keywords

Grapevine, Phaeomoniellachlamydospora, Botryosphaeriaceae, quantitative Real‐Time PCR, TaqMan, Tessior®

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

VOLATILE AND GLYCOSYLATED MARKERS OF SMOKE IMPACT: EVOLUTION IN BOTTLED WINE

Smoke impact in wines is caused by a wide range of volatile phenols found in wildfire smoke. These compounds are absorbed and accumulate in berries, where they may also become glycosylated. Both volatile and glycosylated forms eventually end up in wine where they can cause off-flavors. The impact on wine aroma is mainly attributed to volatile phenols, while in-mouth hydrolysis of glycosylated forms may be responsible for long-lasting “ashy” aftertastes (1).

Evolution of oak barrels C-glucosidic ellagitannins

During oak wood contact, wine undergoes important modifications that modulate its organoleptic quality and complexity, including its aroma, structure, astringency, bitterness and color. Vescalagin and castalagin are the two main C-glucosidic ellagitannins found in oak wood used for wine aging wood but lyxose/xylose derivatives (grandinin and roburin e) and dimeric forms (roburins a,b, c and d) are also present. The presence of several hydroxyl groups in the ortho-positions at the periphery of the structure of the ellagitannin isomers allows these molecules to undergo oxidation or condensation reactions with other compounds.

Oenotannins addition in wine: can be the modulation of redox potential predictable?

The purpose of this research was to study the interaction between oenotannins and wine matrix in order to design a targeted oenotannins addition for modulating the redox status of wine. It is in fact known that oenotannins can regulate the redox potential of musts and wines since they are electroactive substances (1).

Late pruning, an alternative for rainfed vine varieties facing new climatic conditions

In Chile there is a dry farming area known as a traditional wine region, where varieties brought by the Spanish conquerors still persist. These varieties, in general, are cultivated under traditional systems, with low use of technical and economic resources, and low profitability for their grapes and wines. In this region, as in other wine grape growing areas, climatic conditions have changed significantly in recent decades. In particular, the occurrence of spring frosts, when bud break has already begun, have generated significant losses for these growers.

OENOLOGICAL STRATEGIES FOR THE REMOVAL OF PINKING IN WHITE WINE

The pinking of in white wine is the turning of color from yellow to salmon hue. White wines obtained from certain grape varieties (e.g. Chardonnay, Sauvignon blanc, Riesling, Trebbiano di Lugana) showed to be susceptible to pinking [1] that has been evaluated by an assay providing the addition of hydrogen peroxide. Even if its appearance does not seem to affect the sensory properties [2], strategies are necessary for its removal. Nowadays, the treatment with polyvinylpolipirroline (PVPP) was reported to significantly decrease the pink color [3].