Macrowine 2021
IVES 9 IVES Conference Series 9 Using elicitors in different grape varieties. Effect over their phenolic composition

Using elicitors in different grape varieties. Effect over their phenolic composition

Abstract

Phenolic compounds are very important in crop plants and have been the subject of a large number of studies. Three main reasons can be cited for optimizing the level of phenolic compounds in crop plants: their physiological role in plants, their technological significance for food processing, and their nutritional characteristics1 Indeed, an enormous diversity of phenolic antioxidants is found in fruits and vegetables, and their presence and roles can be affected or modified by several pre- and postharvest cultural practices and/or food processing technologies (Ruiz-García et al. 2012, Goldman et al. 1999, Tudela et al. 2002). In winegrapes, the technological importance of phenolic compounds, mainly flavonoids, is well-known. They are responsible for the color of wines, especially anthocyanins (colored pigments responsible for the chromatic characteristics of red wines), tannins (responsible for the long-term stability of red wine color), and flavonols (compounds that may influence wine color through copigmentation). Moreover, they influence on other organoleptic properties such as astringency, bitterness, and body (Ruiz-García et al. 2012). Several techniques have been applied to improve the phenolic content of grapes. Leaving aside genetically modified plants, which are not allowed by the regulations of most countries, the most common techniques are related to cultural practices: pruning (González-Neves et al. 2002, Pérez-Lamela et al. 2007), cluster thinning (Fanzone et al. 2012, Soufleros et al. 2011), leaf removal (Gatti et al. 2012), and deficit irrigation (De la Hera et al. 2005, Koundouras et al. 2009). Another, more recent, technique for the same purpose is the use of elicitors, which are growing in interests. Elicitors are phytochemicals that do not kill pathogens themselves but trigger plant mechanisms that improve pathogen resistance, among them an increase in the levels of phenolic compounds (Vitallini et al. 2011), which are not only toxic to pathogens but also the precursors of disease-resistant material such as lignin (Pan and Liu 2011). This observed increase in phenolic compounds after the application of some elicitors justifies the interest in their application in fruits and vegetables. In our study, the effect of the application of benzhothiadiazol (BTH) and methyl jasmonate at veraison on the phenolic composition of grapes from three varieties (Monastrell, Cabernet Sauvignon and Merlot) was studied at harvest and after that, in wines at the end of alcoholic fermentation.The results showed that the effects of the treatments differed in the three varieties for the several phenolic compounds.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Rocio Gil

*IMIDA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

New molecular evidence of wine yeast-bacteria interaction unraveled by untargeted metabolomic profiling

Bacterial malolactic fermentation (MLF) has a considerable impact on wine quality. The yeast strain used for primary fermentation can consistently stimulate (MLF+ phenotype) or inhibit (MLF- phenotype) malolactic bacteria and the MLF process as a function of numerous winemaking practices, but the molecular evidence behind still remains a mystery. In this study, such evidence was elucidated by the direct comparison of extracellular metabolic profiles of MLF+ and MLF- yeast phenotypes. Untargeted metabolomics combining ultrahigh-resolution FT-ICR-MS analysis, powerful machine learning methods and a comprehensive wine metabolite database, discovered around 800 putative biomarkers and 2500 unknown masses involved in phenotypic distinction.

The impact of branched chain and aromatic amino acids on fermentation kinetics and aroma biosynthesis by wine yeast Saccharomyces cerevisiae

One of the major determinants of wine quality is the aroma. Wine aroma is the human perception of the matrix of grape and yeast derived volatiles and their interaction that contribute to flavour wine. Most common are higher alcohols, ester and aldehydes. In previous studies the formation of characteristic volatile compounds have been linked to the metabolism of branched-chain and aromatic amino acids
(BCAAs) in synthetic grape must. Here we report on an investigation to assess the impact of the initial amino acid concentration on the production of aroma compounds by the industrial yeast VIN13 grown in both synthetic and real grape musts.

Effect of non-Saccharomyces yeast and lactic acid bacteria on selected sensory attributes and polyphenols of Syrah wines

Consumers predominantly use visual, aromatic and texture cues as quality/preference indicators to describe olfactory sensations. In this study, the effect of micro-organism in wine production was investigated using analytical and sensory techniques to achieve relevant analytical characterisation. Selected anthocyanins, flavan-3-ols, flavonols and phenolic acids were quantified in Syrah wines using RP-HPLC-DAD. Standard oenological parameters were also measured. Syrah grape must was fermented with various combinations of Saccharomyces cerevisiae (S. cerevisiae) and non-Saccharomyces (Metschnikowia pulcherrima or Hanseniaspora uvarum) yeasts, which was followed by sequential inoculation of lactic acid bacteria (LAB) (Oenococcus oeni or Lactobacillus plantarum).

Capture depletion of grapevine DNA: an approach to advance the study of microbial community in wine

The use of next-generation sequencing (NGS) has helped understand microbial genetics in oenology. Current studies mainly focus on barcoded amplicon NGS but not shotgun sequencing, which is useful for functional analyses. Since the high percentage of grapevine DNA conceals the microbial DNA in must, the majority of sequencing data is wasted in bioinformatic analyses. Here we present capture depletion of grapevine whole genome DNA.

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept.