Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Abstract

Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines. MATERIAL AND METHODS: Macabeo grapes were harvested at the appropriate maturity and pressed. The grape juice was immediately sulfited and pectinolytic enzymes were added to facilitate settling. After 24 h, clean grape juices (around 70 L each) were racked into nine 100-L stainless steel tanks and were fermented under controlled temperature (16-18 ºC) with selected yeasts. Three tanks were considered as controls whereas other 6 were supplemented with 40 g/hL of 2 inactive yeasts (3 with Optiwhite and 3 with Optimumwhite; Lallemand Inc., Montreal, Canada). Once the alcoholic fermentation was finished, wines were sulfited, racked and cold stabilized. Proteins were analyzed by HRSEC-DAD [3], polysaccharides by HRSEC-RID [4] and foaming properties by the Mosalux procedure [5]. RESULTS: Both inactive yeasts increased the protein and polysaccharide concentration of the base wines in comparison with the non-supplemented control wines. Optiwhite was more effective for enriching polysaccharide concentration whereas Optimumwhite was more effective for enriching protein concentration. Regardless the foam properties, supplementation with Optiwhite originated base wines with a significant higher value of foamability (Hm) than the control. The persistence of the foam (Hs) also tended to be higher but this increase was not statistically significant. Optimumwhite also tended to increase both parameters (Hm and Hs) but none of these differences was statistically significant. CONCLUSIONS: The supplementation with inactive yeasts is a useful tool to increase protein and polysaccharide concentration of base wines and also to improve its foam properties.

REFERENCES: [1] Esteruelas M, González-Royo E, Kontoudakis N, Orte A, Cantos A, Canals JM, Zamora F (2015) J. Sci. Food Agric., 95, 2071-2080 [2] Pozo-Bayón MA, Andujar-Ortiz I, Alcalde-Hidalgo JM, Martín-Alvárez PJ, Moreno-Arribas MV (2009) J. Agric. Food Chem. 57, 10784-10792 [3] Canals JM, Arola L, Zamora F (1998) Am. J. Enol. Vitic., 49, 383-388 [4] Ayestaran B, Guadalupe Z, Leon D (2004) Anal. Chim. Acta. 513:29-39 [5] Maujean A, Poinsaut P, Dantan H, Brissonet F, Cossiez E (1990) Bull. OIV, 711-712, 405-426

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Fernando Zamora*, Elena González-Royo, Joan Miquel Canals, José María Heras, Laura Medina, Nathalie Sieczkowski

*Universitat Rovira i Virgili

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Use of chitosan as a secondary antioxidant in juices and wines

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.

Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Wine industry is looking forward for innovative, safe and eco-friendly antimicrobial products allowing the reduction of chemical treatments in the grape defense and the winemaking process that can affect negatively the quality of the product. Ozone has been tested in food industry giving good results in preventing fungi and bacteria growth on a wide spectrum of vegetables and fruits, due to its oxidant activity and ability to attack numerous cellular constituents. Ozone leaves no chemical residues on the food surface, decomposing itself rapidly in oxygen. Gaseous ozone has been already tested for table grapes storage and on wine grapes during withering.

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T

Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Anthocyanin accumulation and extractability were studied in Tannat, Cabernet Sauvignon and Merlot grapes produced in the south of Uruguay in two consecutive seasons. Typical cultivation situations employed in the region for each variety were considered. A follow-up was carried out, considering 60 plants per vineyard, and the harvest was determined according to the technological indices of maturity. Samples of grapes were taken in duplicate in each vineyard periodically along grape maturation. The basic composition, polyphenolic potential and anthocyanin extractability were determined. Also, half of grapes were frozen and later peeled; skin extractions over 24 hs with a solution of 12% ethanol and pH 3.2 were carried out. The anthocyanin contents of the extracts obtained were determined by HPLC-DAD. The levels of anthocyanins reached the highest values before technological maturity. Anthocyanin extractability had a decrease during grape maturation.

To a better understanding of the impact of vine nitrogen status on volatile thiols from plot to transcriptome level

Volatile thiols contribute largely to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless and non-volatile precursors found in the berry and the must. The effect of vine nitrogen status on 3SH and 4MSP in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) was investigated in this study.