Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Effect of supplementation with inactive yeast during alcoholic fermentation in base wine for sparkling

Abstract

Foam stability of sparkling wines is significantly favored by the presence of surface active agents such as proteins and polysaccharides [1]. For that reason, the renowned sparkling wines are aged after the second fermentation in contact with the lees for several months (even years). Thereby wines are enriched in these macromolecules due to yeast autolysis. Since this practice is slow and costly, winemakers are seeking for alternative procedures to increase their concentration in base wines. In that sense, the supplementation with inactive yeast during alcoholic fermentation has been proposed [2]. The aim of this study was to determine whether this new strategy is really useful for enriching base wines in macromolecules and for improving foam properties of the base wines. MATERIAL AND METHODS: Macabeo grapes were harvested at the appropriate maturity and pressed. The grape juice was immediately sulfited and pectinolytic enzymes were added to facilitate settling. After 24 h, clean grape juices (around 70 L each) were racked into nine 100-L stainless steel tanks and were fermented under controlled temperature (16-18 ºC) with selected yeasts. Three tanks were considered as controls whereas other 6 were supplemented with 40 g/hL of 2 inactive yeasts (3 with Optiwhite and 3 with Optimumwhite; Lallemand Inc., Montreal, Canada). Once the alcoholic fermentation was finished, wines were sulfited, racked and cold stabilized. Proteins were analyzed by HRSEC-DAD [3], polysaccharides by HRSEC-RID [4] and foaming properties by the Mosalux procedure [5]. RESULTS: Both inactive yeasts increased the protein and polysaccharide concentration of the base wines in comparison with the non-supplemented control wines. Optiwhite was more effective for enriching polysaccharide concentration whereas Optimumwhite was more effective for enriching protein concentration. Regardless the foam properties, supplementation with Optiwhite originated base wines with a significant higher value of foamability (Hm) than the control. The persistence of the foam (Hs) also tended to be higher but this increase was not statistically significant. Optimumwhite also tended to increase both parameters (Hm and Hs) but none of these differences was statistically significant. CONCLUSIONS: The supplementation with inactive yeasts is a useful tool to increase protein and polysaccharide concentration of base wines and also to improve its foam properties.

REFERENCES: [1] Esteruelas M, González-Royo E, Kontoudakis N, Orte A, Cantos A, Canals JM, Zamora F (2015) J. Sci. Food Agric., 95, 2071-2080 [2] Pozo-Bayón MA, Andujar-Ortiz I, Alcalde-Hidalgo JM, Martín-Alvárez PJ, Moreno-Arribas MV (2009) J. Agric. Food Chem. 57, 10784-10792 [3] Canals JM, Arola L, Zamora F (1998) Am. J. Enol. Vitic., 49, 383-388 [4] Ayestaran B, Guadalupe Z, Leon D (2004) Anal. Chim. Acta. 513:29-39 [5] Maujean A, Poinsaut P, Dantan H, Brissonet F, Cossiez E (1990) Bull. OIV, 711-712, 405-426

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Fernando Zamora*, Elena González-Royo, Joan Miquel Canals, José María Heras, Laura Medina, Nathalie Sieczkowski

*Universitat Rovira i Virgili

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Novel analytical technologies for wine fingerprinting in and beyond the laboratory

For characterization, sensory designing and authentication rapid analytical technologies have become available. Some, like Proton Transfer Reaction Mass Spectrometry allow a rapid spectrum of the volatile compounds of wines. Combined with chemometrics wines can be characterized. The same approach can be used to calculate the results of virtual mixtures and allow formulation of constant quality blends. Other new techniques and portable devices based on spectroscopy allow measurements on production sites and in grocery stores, even for the smart consumer. We will present some examples of the application of these techniques for authentication of wines, both in the laboratory and on site.

Enological evaluation of the attitude of the grapevine fumin to give varietal wines

Initiatives have been ongoing in recent years to safeguard biodiversity in the oenological sector via a process of enhancement of ancient varieties, under a pressure of a market strongly oriented towards production deriving from native vines of specific geographical zones. In that sense, Aosta Valley
(Italy) has raised the need to preserve and characterize its minority vine varieties which have the potentiality to give varietal wines. Fumin represents the 7% of the production of the region with 16 hectares of vineyards and 753 hectolitres of derived wine. Due to its large phenolic potential, strong astringency and deep colour, it has long been, and is still today, assembled or blended with other varieties as occurs, for example, for the Torrette.

Flavanol glycosides in grapes and wines : the key missing molecular intermediates in condensed tannin biosynthesis ?

Polyphenols are present in a wide variety of plants and foods such as tea, cacao and grape1. An important sub-class of these compounds is the flavanols present in grapes and wines as monomers (e.g (+)-catechin or (-)-epicatechin), or polymers also called condensed tannins or proanthocyanidins. They have important antioxidant properties2 but their biosynthesis remains partly unknown. Some recent studies have focused on the role of glycosylated intermediates that are involved in the transport of the monomers and may serve as precursors in the polymerization mechanism3, 4. The global objective of this work is to identify flavanol glycosides in grapes or wines, describe their structure and determine their abundance during grape development and in wine.

Field-grown Sauvignon Blanc berries react to increased exposure by controlling antioxidant homeostasis and displaying UV acclimation responses that are influenced by the level of ambient light

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).

Ellagitannins and flavano-ellagitannins: concentration ranges in different areas and sensory evaluation

C-Glucosidic ellagitannins, which are the main polyphenolic compounds in oak heartwood, are extracted by wine during aging in oak barrels. Although such maturing of alcoholic beverages in oak barrels is a multi-centennial practice, very little is known on the impact of these ellagitannins on the organoleptic properties of red wine. The objectives of the present investigation were (i) to isolate oak ellagitannins and to hemisynthesize some made-in-wine flavano-ellagitannins, such as acutissimin A; (ii) to analyse their concentration ranges depending on the cultivar area and (iii) to evaluate their sensory impact on the basis of their human threshold concentrations and dose/response relationships in different types of solutions.