Macrowine 2021
IVES 9 IVES Conference Series 9 Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

Abstract

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide. Most Muscat Bailey A wines have unique characters, such as a light mouth feel which is derived from the low concentration of proanthocyanidins (1). In addition, Muscat Bailey A grapes also biosynthesize and accumulate furaneol, suggesting that furaneol might be essential for the characteristic flavor of this wine (2). The previous study identified furaneol glucoside from the juice of Muscat Bailey A, using high-performance liquid chromatography–tandem mass spectrometry, and this was followed by its isolation from some fruits such as strawberry and tomato (3). Furaneol glucoside is a significant ‘aroma precursor of wine’ because furaneol is liberated from it during alcoholic fermentation. In this study, we have identified a glucosyltransferase gene from Muscat Bailey A (UGT85K14), which is responsible for the glucosylation of furaneol (4). Recombinant UGT85K14 expressed in Escherichia coli is able to transfer a glucose moiety from UDP-glucose to the hydroxy group of furaneol, indicating that this gene might be UDP-glucose: furaneol glucosyltransferase in Muscat Bailey A. Furaneol glucoside content in Muscat Bailey A berry during maturation might be controlled by the expression of UGT85K14 along with the biosynthesis of furaneol. On the other hand, UGT85K14 was expressed in the representative grape cultivars regardless of species. In addition to Muscat Bailey A, the corresponding genes from Pinot Noir (V. vinifera) and Concord (V. labrusca) have been identified and characterized. Sequence analysis and the characterization of recombinant proteins demonstrated that furaneol glucoside content was regulated by the biosynthesis of furaneol in grape species (V. vinifera and V. labrusca), and both species might have evolved and diverged after the molecular evolution of this gene.

References
1. Ichikawa, M. et al. (2011) Food Sci. Technol. Res. 17, 335-339. 2. Kobayashi, H. et al. (2013) Vitis 52, 9-11. 3. Sasaki, K. et al. (2015) Am. J. Enol. Vitic. 66, 91-94. 4. Sasaki, K. et al. (2015) J. Exp. Bot. 66, 6167-6174.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Kanako Sasaki*, Gen Ikoma, Hideki Takase, Hironori Kobayashi, Hironori Matsuo, Ryoji Takata

*Research Laboratories for Wine

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Extraction of polyphenols from grape marc by supercritical fluid extraction (SFE) and evaluation of their ‘bioavailability’ as dietary supplements

In the winemaking process, several compounds that remain in the grape skins and seeds after the fermentation stage are bioactive-compounds (substances with potential beneficial effects on health) that can be extracted in order to recovery valuable substances with a high commercial value for the cosmetic, food (nutraceuticals) and pharmaceutical industries. The skins contain significant amounts of bioactive substances such as tannins (16-27%) and other polyphenolic compounds (2-6.5%) in particular, catechins, anthocyanins, proanthocyanins, quercetin , ellagic acid and resveratrol.

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Wine industry is looking forward for innovative, safe and eco-friendly antimicrobial products allowing the reduction of chemical treatments in the grape defense and the winemaking process that can affect negatively the quality of the product. Ozone has been tested in food industry giving good results in preventing fungi and bacteria growth on a wide spectrum of vegetables and fruits, due to its oxidant activity and ability to attack numerous cellular constituents. Ozone leaves no chemical residues on the food surface, decomposing itself rapidly in oxygen. Gaseous ozone has been already tested for table grapes storage and on wine grapes during withering.