Macrowine 2021
IVES 9 IVES Conference Series 9 Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

Abstract

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide. Most Muscat Bailey A wines have unique characters, such as a light mouth feel which is derived from the low concentration of proanthocyanidins (1). In addition, Muscat Bailey A grapes also biosynthesize and accumulate furaneol, suggesting that furaneol might be essential for the characteristic flavor of this wine (2). The previous study identified furaneol glucoside from the juice of Muscat Bailey A, using high-performance liquid chromatography–tandem mass spectrometry, and this was followed by its isolation from some fruits such as strawberry and tomato (3). Furaneol glucoside is a significant ‘aroma precursor of wine’ because furaneol is liberated from it during alcoholic fermentation. In this study, we have identified a glucosyltransferase gene from Muscat Bailey A (UGT85K14), which is responsible for the glucosylation of furaneol (4). Recombinant UGT85K14 expressed in Escherichia coli is able to transfer a glucose moiety from UDP-glucose to the hydroxy group of furaneol, indicating that this gene might be UDP-glucose: furaneol glucosyltransferase in Muscat Bailey A. Furaneol glucoside content in Muscat Bailey A berry during maturation might be controlled by the expression of UGT85K14 along with the biosynthesis of furaneol. On the other hand, UGT85K14 was expressed in the representative grape cultivars regardless of species. In addition to Muscat Bailey A, the corresponding genes from Pinot Noir (V. vinifera) and Concord (V. labrusca) have been identified and characterized. Sequence analysis and the characterization of recombinant proteins demonstrated that furaneol glucoside content was regulated by the biosynthesis of furaneol in grape species (V. vinifera and V. labrusca), and both species might have evolved and diverged after the molecular evolution of this gene.

References
1. Ichikawa, M. et al. (2011) Food Sci. Technol. Res. 17, 335-339. 2. Kobayashi, H. et al. (2013) Vitis 52, 9-11. 3. Sasaki, K. et al. (2015) Am. J. Enol. Vitic. 66, 91-94. 4. Sasaki, K. et al. (2015) J. Exp. Bot. 66, 6167-6174.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Kanako Sasaki*, Gen Ikoma, Hideki Takase, Hironori Kobayashi, Hironori Matsuo, Ryoji Takata

*Research Laboratories for Wine

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Use of computational modelling for selecting adsorbents for improved fining of wine

The occurrence of faults and taints in wine, such as those caused by microbial spoilage or various taints, have resulted in significant financial losses to wine producers. The wine industry commits significant financial resources towards fining and taint removal processes each year. Fining involves the addition of one or more adsorptive substrates to juice or wine to bind certain components, thus reducing their concentration [1]. However, these processes are often not selective and can also remove desirable flavour and aroma compounds.

Accumulation of polyphenols in Barbera and Nebbiolo leaves during the vegetative season

Grapevine berries produce thousands of secondary metabolites of diverse chemical nature that have been largely detailed in the past due to their importance for defining wine quality. The wide Vitis vinifera diversity, resulting in thousands of different varieties well detailed in many studies regarding berries, is still not investigated in vegetative organs, leaves in particular. Deepening knowledge related to this aspect could be of great interest for many reasons (for example the possibility of using leaf extract for pharmaceutical, cosmetic and nutrition purposes) but, above all, for understanding the susceptibility of different grapevine varieties to pathogens.

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure.