Macrowine 2021
IVES 9 IVES Conference Series 9 Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

Abstract

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide. Most Muscat Bailey A wines have unique characters, such as a light mouth feel which is derived from the low concentration of proanthocyanidins (1). In addition, Muscat Bailey A grapes also biosynthesize and accumulate furaneol, suggesting that furaneol might be essential for the characteristic flavor of this wine (2). The previous study identified furaneol glucoside from the juice of Muscat Bailey A, using high-performance liquid chromatography–tandem mass spectrometry, and this was followed by its isolation from some fruits such as strawberry and tomato (3). Furaneol glucoside is a significant ‘aroma precursor of wine’ because furaneol is liberated from it during alcoholic fermentation. In this study, we have identified a glucosyltransferase gene from Muscat Bailey A (UGT85K14), which is responsible for the glucosylation of furaneol (4). Recombinant UGT85K14 expressed in Escherichia coli is able to transfer a glucose moiety from UDP-glucose to the hydroxy group of furaneol, indicating that this gene might be UDP-glucose: furaneol glucosyltransferase in Muscat Bailey A. Furaneol glucoside content in Muscat Bailey A berry during maturation might be controlled by the expression of UGT85K14 along with the biosynthesis of furaneol. On the other hand, UGT85K14 was expressed in the representative grape cultivars regardless of species. In addition to Muscat Bailey A, the corresponding genes from Pinot Noir (V. vinifera) and Concord (V. labrusca) have been identified and characterized. Sequence analysis and the characterization of recombinant proteins demonstrated that furaneol glucoside content was regulated by the biosynthesis of furaneol in grape species (V. vinifera and V. labrusca), and both species might have evolved and diverged after the molecular evolution of this gene.

References
1. Ichikawa, M. et al. (2011) Food Sci. Technol. Res. 17, 335-339. 2. Kobayashi, H. et al. (2013) Vitis 52, 9-11. 3. Sasaki, K. et al. (2015) Am. J. Enol. Vitic. 66, 91-94. 4. Sasaki, K. et al. (2015) J. Exp. Bot. 66, 6167-6174.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Kanako Sasaki*, Gen Ikoma, Hideki Takase, Hironori Kobayashi, Hironori Matsuo, Ryoji Takata

*Research Laboratories for Wine

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Bentonite fining in cold wines: prediction tests, reduced efficiency and possibilities to avoid additional fining treatments

Bentonite fining is widely used to prevent protein haze in white wines. Most wineries use laboratory-scale fining trials to define the appropriate amount of bentonite to be used in the cellar. Those pre-tests need to mimic as much as possible the industrial scale fining procedure to determine the exact amount of bentonite necessary for protein stability. Nevertheless it is frequent that, after fining with the recommended amount of bentonite, wines appear still unstable and need an additional fining treatment. It remains a major challenge to understand why the same wine, fined with the same dosage of the same bentonite, achieves stability in the lab, but not in the cellar.

Reduction of herbaceous aromas by wine lactic acid bacteria mediated degradation of volatile aldehydes

Consumers typically prefer wines with floral and fruity aromas over those presenting green-pepper, vegetal or herbaceous notes. Pyrazines have been identified as causatives for herbaceous notes in wines, especially Bordeaux reds. However, pyrazines are not universally responsible for herbaceousness, and several other wine volatile compounds are known to produce distinct vegetal/herbaceous aromas in wines. Specifically, volatile aldehydes elicit sensations of herbaceousness or grassiness and have been described in wines well above their perception thresholds.

Oxygen consumption by diferent oenological tanins in a model wine solution

INTRODUCTION: Oenological tannins are widely used in winemaking to improve some characteristics of wines [1] being the antioxidant properties probably one of the main reasons [2]. However, commercial tannins have different botanical sources and chemical composition [3] which probably determines different antioxidant potential. There are some few references about the antioxidant properties of commercial tannins [4] but none of them have really measured the direct oxygen consumption by them. The aim of this work was to measure the kinetics of oxygen consumption by different commercial tannins in order to determine their real capacities to protect wine against oxygen. MATERIAL AND METHODS: 4 different commercial tannins were used: T1: condensed tannin from grape seeds, T2: gallotannin from chinese gallnuts, T3: ellagitannin from oak and T4: tannin from quebracho containing condensed tannins and ellagitannins.

Effects of bottle closure type on sensory characteristics of Chasselas wines

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.

Ageing of sweet wines: oxygen evolution according to bung and barrel type

Barrel ageing is a crucial step in the wine process because it allows many changes to the wine as enrichment, colour stabilization, clarification and also a slow oxygenation. Effects of the oak barrel have to be known to prevent oxidation of the wine. The type of bung used during ageing is also a parameter to consider. Ageing sweet wines in barrel is a real challenge. These wines may need some oxygen at the beginning of ageing but they should be protected at the end of their maturation, to avoid oxidation.