Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Abstract

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics. Glycosidically bound simple phenols are considered a natural stock of these compounds, because they can be hydrolyzed during the winemaking production releasing the corresponding free forms and constituting a potential contribution to final sensory profile. Adapting the method of Barnaba and colleagues, target and untargeted approaches were developed. On-line purification was performed with a HyperSepTM Retain PEP spe cartridge, the chromatographic separation was performed with an Acquity UPLC BEH C18 analytical column, managing a water-acetonitrile gradient from 5% to 100% of organic solvent. Mass spectra were acquired in full MS-data dependent MS/MS analysis at mass resolving power of 140.000, in negative ion mode and with a heated electrospray. The mass spectrometer operated using following parameters: spray voltage, 2.80 kV; sheath gas flow rate, 30 arbitrary units; capillary temperature, 310 °C. The aim of the study was to increase the understanding of hybrid grape varieties phenolic composition, combining on-line SPE clean-up for reducing matrix interference with an ultra-high liquid chromatography coupled to high resolution mass spectrometry. In particular, the phenolic composition of 4 hybrid (red: Cabernet Cantor and Prior; white: Muscaris and Solaris) and 2 European (red: Merlot; white: Chardonnay) grape varieties was investigated, focusing on free and glycosidically bound simple phenols and considering compounds distribution in pulp, skin and seeds. Through target approach 58 free simple phenols and 7 glucosidic precursors were quantified with quantification limits ranging from 0.001 to 1 mg Kg-1, calibration R2 of 0.99 for over 94% of compounds, and precision (R.S.D.%) always better than 12%. The untargeted approach was aimed to tentatively identify glycosylated precursors of selected free simple phenols in the forms of -hexoside, -pentoside, -hexoside-hexoside, -hexoside-pentoside, -pentoside-hexoside and -pentoside-pentoside derivatives on the basis of accurate mass, isotopic pattern and MS/MS fragmentation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Chiara Barnaba*, Giorgio Nicolini, Mattia Giacomelli, Roberto Larcher, Tiziana Nardin

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Red wine substituted esters involved in fruity aromatic expression: an enantiomeric approach to understand their sensory impact and their pathway formation

Among red wines ethyl esters, those from short hydroxylated and branched-chain aliphatic acids constitute a family with a particular behavior and sensory importance. They have been previously discussed in the literature [1] and recent studies have established that some of them were strongly involved in of red wines’ fruity aroma [2]. As some among them have an asymmetrical carbon atom, it seemed important to separate their different enantiomers to obtain an accurate assessment of their organoleptic impact. Three chiral esters have been identified, presenting alkyl and/or hydroxyle substituants: ethyl 2-hydroxy-4-methylpentanoate, ethyl 2-methylbutanoate, and ethyl 3-hydroxybutanoate.

Colour assessment of port wines using colorimetric and spectrophotometric methods

Colour is an important quality parameter in wines and is the result of a complex mixture of pigments
(including anthocyanins and their derivatives, quinones, xanthyllium compounds, etc.). Red wine colour changes over time as pigments react between themselves and with other wine macromolecules
(particularly polyphenols). During wine tasting, colour is normally assessed on the outer rim of the wine profile in a tilted glass, since most wines are too opaque to be analysed in the middle of the glass. Therefore, depending on the depth of observation considered, the perception of wine colour can be different.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Influence of inactive dry yeast treatments during grape ripening on postharvest berry skin texture parameters and phenolic compounds extractability

Inactive dry yeast treatments in the vineyard are a tool used with the aim to improve the concentration and quality of secondary metabolites in grapes, leading to a better differentiation of the wines made from grapes differently treated. In this work, a foliar spraying treatment with yeast derivatives specifically designed to be used with the patent pending application technology of Lallemand Inc. Canada (LalVigne® Mature, Lallemand Inc., Montreal, Canada) was tested on Vitis vinifera L. cv. Barbera and Nebbiolo black winegrapes. The aim was to evaluate the effect of this treatment on the phenolic compounds accumulation, the skin physical-mechanical properties and the related phenolic extractability. Prior to analysis, the berries were sorted by flotation in order to evaluate their distribution by density class, and to determine the skin texture parameters of berries with different sugar contents, thus understanding also the ripening effect.

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.