Macrowine 2021
IVES 9 IVES Conference Series 9 Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Abstract

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics. Glycosidically bound simple phenols are considered a natural stock of these compounds, because they can be hydrolyzed during the winemaking production releasing the corresponding free forms and constituting a potential contribution to final sensory profile. Adapting the method of Barnaba and colleagues, target and untargeted approaches were developed. On-line purification was performed with a HyperSepTM Retain PEP spe cartridge, the chromatographic separation was performed with an Acquity UPLC BEH C18 analytical column, managing a water-acetonitrile gradient from 5% to 100% of organic solvent. Mass spectra were acquired in full MS-data dependent MS/MS analysis at mass resolving power of 140.000, in negative ion mode and with a heated electrospray. The mass spectrometer operated using following parameters: spray voltage, 2.80 kV; sheath gas flow rate, 30 arbitrary units; capillary temperature, 310 °C. The aim of the study was to increase the understanding of hybrid grape varieties phenolic composition, combining on-line SPE clean-up for reducing matrix interference with an ultra-high liquid chromatography coupled to high resolution mass spectrometry. In particular, the phenolic composition of 4 hybrid (red: Cabernet Cantor and Prior; white: Muscaris and Solaris) and 2 European (red: Merlot; white: Chardonnay) grape varieties was investigated, focusing on free and glycosidically bound simple phenols and considering compounds distribution in pulp, skin and seeds. Through target approach 58 free simple phenols and 7 glucosidic precursors were quantified with quantification limits ranging from 0.001 to 1 mg Kg-1, calibration R2 of 0.99 for over 94% of compounds, and precision (R.S.D.%) always better than 12%. The untargeted approach was aimed to tentatively identify glycosylated precursors of selected free simple phenols in the forms of -hexoside, -pentoside, -hexoside-hexoside, -hexoside-pentoside, -pentoside-hexoside and -pentoside-pentoside derivatives on the basis of accurate mass, isotopic pattern and MS/MS fragmentation.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Chiara Barnaba*, Giorgio Nicolini, Mattia Giacomelli, Roberto Larcher, Tiziana Nardin

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol).

Quantification of red wine phenolics using ultraviolet-visible, near and mid-infrared spectroscopy combined with chemometrics

The use of multivariate statistics to correlate chemical data to spectral information seems as a valid alternative for the quantification of red wine phenolics. The advantages of these techniques include simplicity and cost effectiveness together with the limited time of analysis required. Although many
publications on this subject are nowadays available in the literature most of them only reported feasibility
studies. In this study 400 samples from thirteen fermentations including five different cultivars plus 150
wine samples from a varying number of vintages were submitted to spectrophotometric and chromatographic phenolic analysis.

Anti/prooxidant activity of wine polyphenols in reactions of adrenaline auto-oxidation

Adrenaline (epinephrine) belongs to catecholamine class. It is a neurotransmitter and both a hormone which is released by the sympathetic nervous system and adrenal medulla in response to a range of stresses in order to regulate blood pressure, cardiac stimulation, relaxation of smooth muscles and other physiological processes. Adrenaline exhibits an effective antioxidant capacity (1). However, adrenalin is capable to auto-oxidation and in this case it generates toxic reactive oxygen intermediates and adrenochrome. Under in vitro conditions, auto-oxidation of adrenaline occurs in an alkaline medium (2).