Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Abstract

Cabernet Sauvignon is one of the most important winegrape varieties in Chile. However, temperature raise and decreased rainfall due to climate change can lead to grape quality decrease in certain areas. Amino acids are essential as nitrogen source for yeast but also directly affect grape quality serving as precursors of certain volatile compounds that enhance the wine bouquet. Besides, glutathione is an important tripeptide acting as antioxidant, preventing the appearance of browning pigments in must and exerts a protective effect in volatile compounds. Thus, increasing amino acid and glutathione content on grapes plays a crucial role in winemaking. Nitrogen foliar fertilization can be a useful strategy to achieve this aim because of the quick and efficient assimilation of applied products by plants. Therefore, the effect of different foliar nitrogen applications on must amino acid and glutathione composition on a Cabernet Sauvignon vineyard was studied in order to increase the grape quality. Nitrogen treatments applied to the grapevines were urea (Ur), urea plus sulphur (Ur+S), arginine (Arg) and two commercial foliar fertilizers with amino acids on its composition (Basfoliar Algae (BA) and Nutrimyr Thiols (NT)). Two applications of 1 kg N/ha were sprayed first at the beginning of veraison and two weeks later. Must amino acid and glutathione were analyzed by HPLC-DAD. Oenological parameters for each sample were also determined. Commercial nitrogen sprays increased the amount of aspartic acid, glutamic acid, serine, glutamine, alanine and ornithine (NT), whereas BA increased the content of serine, glutamine, threonine, arginine, methionine and proline. Ur+S treatment had a better assimilation than Ur, increasing content of aspartic acid, glutamic acid, serine, glycine and methionine. Arg treatment did not increase amino acid content, however it increased the amount of easily extractable anthocyanins, total anthocyanins and total polyphenol index. Foliar nitrogen applications of Arg, NT and BA increased considerably the glutathione concentration, from 2.62 mg/L in control samples to 26.48, 41.51 and 27.6 mg/L in Arg, NT and BA musts, respectively. These findings have oenological and viticultural interest for improving grape quality by enhancing must amino acid composition in high proline accumulating varieties as Cabernet Sauvignon.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Ana Gonzalo-Diago, Ana Martínez-Gil, Gaston Gutiérrez-Gamboa, Yerko Moreno-Simunovic

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Innovations in the use of bentonite in enology: interactions with grape and wine proteins, colloids, polyphenols and aroma compounds.

The use of bentonite in oenology rounds around the limpidity and the stability that determine consumer acceptability. As a matter of fact, the haze formation in wine reduces its commercial value and makes it unacceptable for sale. Stabilization treatments are, therefore, essential to ensure a long-time limpidity and to forecast the formation of deposits in the bottle. Bentonite that is normally used in oenology for clarifying-fining purpose, shows a natural clay-based mineral structure allowing it to swell and to jelly in water and hence in must and wine.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Impact of industrial-scale serial filtration on macromolecules in red wines

Filtration is a critical step in ensuring the clarity and microbial stability of wine prior to bottling. However the process of filtering potentially reduces red wine quality by removing some of the macromolecules that contribute to the texture of the wine. Commercial red wines, Cabernet Sauvignon (CAS) and Shiraz (SHZ), of two vintages and two grades (premium grade wines from the older vintage: CAS13 and SHZ13; and standard grade wines from a younger vintage: CAS14 and SHZ14) were filtered through industrial-scale commercial filtration units prior to bottling. Samples were taken before and after cross-flow filtration, lenticular filters, 0.65 µm and 0.45 µm pore size nylon membrane filters. The concentration and composition of macromolecules, including tannins and polysaccharides, were measured in all samples as well as particle size distribution and wine colour.

Correlations between sensory characteristics and colloidal content in dry white wines

Must clarification is an important step occurring just after grape extraction in the elaboration of white wine, consisting in a solid-liquid separation. Traditionally, low must turbidity, around 50-150 NTU, is generally reached in white winemaking in order to prevent reductive aromas and facilitating alcoholic fermentation. Alternatively, a higher turbidity (300 NTU or above) can be sought for reasons such as a better expression of grapes identity (terroir), or for getting a must matrix that could supposedly lead to wines having greater ageing potential.

Mean polymerization degree of proanthocyanidins of grape seeds, skins and wines from Agiorgitiko (cv. Vitis vinifera): Differences among vintages

Grape phenolic compounds are very important constituents of red wine because, in addition to their antioxidant properties, they contribute to color, astringency and bitterness, oxidation reactions, interactions with proteins and ageing behavior of wines. The aim of our study was to assess the structural characteristics of grape and wine proanthocyanidins of Agiorgitiko variety and to evaluate the influence of the vintage year. Twelve vineyard locations were designated in the Nemea wine region. For three consecutive years (2012-2014), the grapes were harvested at technological maturity and the method of phloroglucinolysis was employed to determine the mean degree of polymerization (mDP) and subunit composition of the samples.