Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Abstract

Cabernet Sauvignon is one of the most important winegrape varieties in Chile. However, temperature raise and decreased rainfall due to climate change can lead to grape quality decrease in certain areas. Amino acids are essential as nitrogen source for yeast but also directly affect grape quality serving as precursors of certain volatile compounds that enhance the wine bouquet. Besides, glutathione is an important tripeptide acting as antioxidant, preventing the appearance of browning pigments in must and exerts a protective effect in volatile compounds. Thus, increasing amino acid and glutathione content on grapes plays a crucial role in winemaking. Nitrogen foliar fertilization can be a useful strategy to achieve this aim because of the quick and efficient assimilation of applied products by plants. Therefore, the effect of different foliar nitrogen applications on must amino acid and glutathione composition on a Cabernet Sauvignon vineyard was studied in order to increase the grape quality. Nitrogen treatments applied to the grapevines were urea (Ur), urea plus sulphur (Ur+S), arginine (Arg) and two commercial foliar fertilizers with amino acids on its composition (Basfoliar Algae (BA) and Nutrimyr Thiols (NT)). Two applications of 1 kg N/ha were sprayed first at the beginning of veraison and two weeks later. Must amino acid and glutathione were analyzed by HPLC-DAD. Oenological parameters for each sample were also determined. Commercial nitrogen sprays increased the amount of aspartic acid, glutamic acid, serine, glutamine, alanine and ornithine (NT), whereas BA increased the content of serine, glutamine, threonine, arginine, methionine and proline. Ur+S treatment had a better assimilation than Ur, increasing content of aspartic acid, glutamic acid, serine, glycine and methionine. Arg treatment did not increase amino acid content, however it increased the amount of easily extractable anthocyanins, total anthocyanins and total polyphenol index. Foliar nitrogen applications of Arg, NT and BA increased considerably the glutathione concentration, from 2.62 mg/L in control samples to 26.48, 41.51 and 27.6 mg/L in Arg, NT and BA musts, respectively. These findings have oenological and viticultural interest for improving grape quality by enhancing must amino acid composition in high proline accumulating varieties as Cabernet Sauvignon.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Ana Gonzalo-Diago, Ana Martínez-Gil, Gaston Gutiérrez-Gamboa, Yerko Moreno-Simunovic

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Comparison of fortified, sfursat and passito winemaking techniques for the enhancement of the oenological potential of the black grape cultivar Moscato nero d’Acqui (Vitis vinifera L.)

One of the key factors of the economical development of viticulture and wine industry in specific limited areas is the exploitation of ancient, local grape varieties. Therefore, in recent years the growing interest to rediscover minor varieties, previously cultivated, has promoted many studies. With this regard, the focus of this study was the Vitis vinifera L. cultivar Moscato nero d’Acqui, nowadays found only in old vineyards in the Acqui zone (North-West Italy). In particular, the aims of this work were: i) to investigate secondary metabolites profile of the grapes, and ii) to evaluate the attitude to the production of special wines.

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Influence of wood chips addition during alcoholic fermentation on wine phenolic composition

This study investigates the effect of wood chips addition during the alcoholic fermentation on the phenolic
composition of the produced wines. A series of wood chips, originating from American, French, Slavonia
oak and Acacia were added at the beginning of wine alcoholic fermentation. Besides, a mixture consisting
of 50% French and 50% Americal oak chips were added during the experimentation. The wine samples
were analyzed one month after the end of malolactic fermentation, examining various chemical
parameters such as total anthocyanins, total phenolic content, tannins combined with protein (BSA) and
ellagitannin content.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.