Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Effect of different foliar nitrogen applications on the must amino acids and glutathione composition in Cabernet Sauvignon vineyard

Abstract

Cabernet Sauvignon is one of the most important winegrape varieties in Chile. However, temperature raise and decreased rainfall due to climate change can lead to grape quality decrease in certain areas. Amino acids are essential as nitrogen source for yeast but also directly affect grape quality serving as precursors of certain volatile compounds that enhance the wine bouquet. Besides, glutathione is an important tripeptide acting as antioxidant, preventing the appearance of browning pigments in must and exerts a protective effect in volatile compounds. Thus, increasing amino acid and glutathione content on grapes plays a crucial role in winemaking. Nitrogen foliar fertilization can be a useful strategy to achieve this aim because of the quick and efficient assimilation of applied products by plants. Therefore, the effect of different foliar nitrogen applications on must amino acid and glutathione composition on a Cabernet Sauvignon vineyard was studied in order to increase the grape quality. Nitrogen treatments applied to the grapevines were urea (Ur), urea plus sulphur (Ur+S), arginine (Arg) and two commercial foliar fertilizers with amino acids on its composition (Basfoliar Algae (BA) and Nutrimyr Thiols (NT)). Two applications of 1 kg N/ha were sprayed first at the beginning of veraison and two weeks later. Must amino acid and glutathione were analyzed by HPLC-DAD. Oenological parameters for each sample were also determined. Commercial nitrogen sprays increased the amount of aspartic acid, glutamic acid, serine, glutamine, alanine and ornithine (NT), whereas BA increased the content of serine, glutamine, threonine, arginine, methionine and proline. Ur+S treatment had a better assimilation than Ur, increasing content of aspartic acid, glutamic acid, serine, glycine and methionine. Arg treatment did not increase amino acid content, however it increased the amount of easily extractable anthocyanins, total anthocyanins and total polyphenol index. Foliar nitrogen applications of Arg, NT and BA increased considerably the glutathione concentration, from 2.62 mg/L in control samples to 26.48, 41.51 and 27.6 mg/L in Arg, NT and BA musts, respectively. These findings have oenological and viticultural interest for improving grape quality by enhancing must amino acid composition in high proline accumulating varieties as Cabernet Sauvignon.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Teresa Garde-Cerdan*, Ana Gonzalo-Diago, Ana Martínez-Gil, Gaston Gutiérrez-Gamboa, Yerko Moreno-Simunovic

*Instituto de Ciencias de la Vid y del Vino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.

On the losses of dissolved CO2 from laser-etched champagne glasses under standard tasting conditions

Under standard champagne tasting conditions, the complex interplay between the level of dissolved CO2 found in champagne, its temperature, the glass shape, and the bubbling rate, definitely impacts champagne tasting by modifying the neuro-physico-chemical mechanisms responsible for aroma release and flavor perception. Based on theoretical principles combining heterogeneous bubble nucleation, ascending bubble dynamics and mass transfer equations, a global model is proposed (depending on various parameters of both the wine and the glass itself), which quantitatively provides the progressive losses of dissolved CO2 from laser-etched champagne glasses.

Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Fermentative aromas (especially esters and higher alcohols) highly impact the organoleptic profile of young and white wines. The production of these volatile compounds depends mainly on temperature and Yeast Available Nitrogen (YAN) content in the must. Available dynamic models predict the main reaction
(bioconversion of sugar into ethanol and CO2 production) but none of them considers the production kinetics of fermentative aroma compounds during the process of fermentation. We determined the production kinetics of the main esters and higher alcohols for different values of initial YAN content and temperature, using an innovative online monitoring Gas Chromatography device.

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality.

Evaluating South African Chenin blanc wine styles using an LC-MS screening method

Sorting Chenin blanc is one of the most important white wine cultivars in South Africa. It has received a lot of attention and accolades in the past years and more research than ever is dedicated to this versatile cultivar. According to the Chenin blanc association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded
(RRU), and Rich and Ripe Wooded (RRW). They are traditionally established with the aid of expert sensory evaluation, but the cost and the (subjective) human factor are aspects to be taken into account. A more objective and possibly robust way of assessing and attributing these styles can be the use of chemical analysis.