Macrowine 2021
IVES 9 IVES Conference Series 9 Monitoring of Pesticide Residues from Vine to Wine

Monitoring of Pesticide Residues from Vine to Wine

Abstract

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines. Although no Maximum Residue Limits (MRLs) are set so far for the wine, powerful analysis methods have to be developed in order to detect low levels of compounds and prohibited molecules in particular. Moreover, organic winegrowers are concerned about contamination problems that may arise in the field or during the winemaking. In this work, a liquid chromatography – tandem mass spectrometry (LC-MS/MS) method is used to monitor about 190 pesticide residues. The extraction step is carried out either by QuEChERS (Quick, Easy, Cheap, Efficient, Rugged and Safe) or by SPE (Solid-Phase Extraction) for liquid samples. Optimisations were performed for the extraction of analytes from vine leaves (grinding, QuEChERS extraction and purification step) and validation parameters were controlled for different matrices (vine leaves, grapes, wines, lees) in terms of precision and accuracy. Several experiments were then conducted to monitor pesticide residues. For two vintages, vine leaves were analysed during the summer for a conventional plot as well as for an organic plot next to it. Sampling was done before and 48 hours after each phytosanitary treatment to evaluate contamination of the organic plot and the persistence of residues over time. Grapes, musts and wines were also analysed and when possible compared to the applied phytosanitary scheme to evaluate the persistence of compounds used in the vineyard. Sampling was also carried out all along the vinification process, from ripe berries to the wine during ageing in order to observe on one hand if contamination phenomena took place and eventually detect critical steps and on the other hand to estimate the transfer of active substances all along the process. Preliminary experiments were also conducted on the effect of alcoholic fermentation on pesticide residues at laboratory scale. Persistence and transfer are variable depending on molecules.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Céline Franc*, Gilles de Revel

*Université de Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

Light-struck taste in white wine: enological approach for its prevention

Light-struck taste is a defect prevalent in white wines bottled in clear glass light-exposed for a considerable amount of time leading to a loss of color and appearance of sulfur-like odors. The reaction involves riboflavin (RF), a highly photosensitive compound that undergoes to intermolecular photoreduction by the uptake of two electron equivalents from an external donor, the methionine. The reaction includes different steps forming methional which is extremely unstable and decomposes to methane thiol and acrolein. The reaction of two molecules of methane thiol yields dimethyl disulfide. Methane thiol is highly volatile, has a low perception threshold (2 to 10 µg/L in wine) and confers aroma-like rotten eggs or cabbage.

Development of a new sustainable filtering media for wine and beer clarification and sterilisation

Different separation techniques are frequently used during vinification process. Nowadays, clarification and microbiological stabilization of wine or beer can be done using precoat filters or crossflow filters to remove yeast and bacteria. Kieselguhr powders are the most used filter aids for precoat filtration. Their crystalline structure and their pulverulent nature induce ecotoxicological risks when used. Moreover, regeneration and reuse of these filter aids is not efficient and the filtration waste requires cost effective retreatment.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).