Macrowine 2021
IVES 9 IVES Conference Series 9 Monitoring of Pesticide Residues from Vine to Wine

Monitoring of Pesticide Residues from Vine to Wine

Abstract

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines. Although no Maximum Residue Limits (MRLs) are set so far for the wine, powerful analysis methods have to be developed in order to detect low levels of compounds and prohibited molecules in particular. Moreover, organic winegrowers are concerned about contamination problems that may arise in the field or during the winemaking. In this work, a liquid chromatography – tandem mass spectrometry (LC-MS/MS) method is used to monitor about 190 pesticide residues. The extraction step is carried out either by QuEChERS (Quick, Easy, Cheap, Efficient, Rugged and Safe) or by SPE (Solid-Phase Extraction) for liquid samples. Optimisations were performed for the extraction of analytes from vine leaves (grinding, QuEChERS extraction and purification step) and validation parameters were controlled for different matrices (vine leaves, grapes, wines, lees) in terms of precision and accuracy. Several experiments were then conducted to monitor pesticide residues. For two vintages, vine leaves were analysed during the summer for a conventional plot as well as for an organic plot next to it. Sampling was done before and 48 hours after each phytosanitary treatment to evaluate contamination of the organic plot and the persistence of residues over time. Grapes, musts and wines were also analysed and when possible compared to the applied phytosanitary scheme to evaluate the persistence of compounds used in the vineyard. Sampling was also carried out all along the vinification process, from ripe berries to the wine during ageing in order to observe on one hand if contamination phenomena took place and eventually detect critical steps and on the other hand to estimate the transfer of active substances all along the process. Preliminary experiments were also conducted on the effect of alcoholic fermentation on pesticide residues at laboratory scale. Persistence and transfer are variable depending on molecules.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Céline Franc*, Gilles de Revel

*Université de Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Sensory and nephelometric analysis of tannin fractions obtained by ultrafiltration of red wines

The assessment of red wine mouthfeel relies primarily on the sensory description of its tannic properties. This evaluation could be improved by gaining a better understanding of the physicochemical properties of these tannins. Hence, the objectives of the present study were threefold: (1) to gain an insight into the sensory properties of subpopulations of proanthocyanidic tannins of different molecular sizes obtained through several ultrafiltration steps, (2) to quantify the kinetics of haze formation of these proanthocyanidic tannins in a dynamic polyvinylpyrrolidone (PVP) precipitation test, (3) to determine whether a correlation exists between the sensory and the precipitation data.

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

New molecular evidence of wine yeast-bacteria interaction unraveled by untargeted metabolomic profiling

Bacterial malolactic fermentation (MLF) has a considerable impact on wine quality. The yeast strain used for primary fermentation can consistently stimulate (MLF+ phenotype) or inhibit (MLF- phenotype) malolactic bacteria and the MLF process as a function of numerous winemaking practices, but the molecular evidence behind still remains a mystery. In this study, such evidence was elucidated by the direct comparison of extracellular metabolic profiles of MLF+ and MLF- yeast phenotypes. Untargeted metabolomics combining ultrahigh-resolution FT-ICR-MS analysis, powerful machine learning methods and a comprehensive wine metabolite database, discovered around 800 putative biomarkers and 2500 unknown masses involved in phenotypic distinction.

Impact of non-fruity compounds on red wines fruity aromatic expression: the role of higher alcohols

A part, at least, of the fruity aroma of red wines is the consequence of perceptive interactions between various aromatic compounds, particularly ethyl esters and acetates, which may contribute to the perception of fruity aromas, specifically thanks to synergistic effects.1,2 The question of the indirect impact of non-fruity compounds on this particular aromatic expression has not yet been widely investigated. Among these compounds higher alcohols (HA) represent the main group, from a quantitative standpoint, of volatiles in many alcoholic beverages. Moreover, some bibliographic data suggested their contribution to the aromatic complexity by either increasing or masking flavors of wine, depending of their concentrations.

Fining-Derived Allergens in Wine: from Detection to Quantification

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition).