Macrowine 2021
IVES 9 IVES Conference Series 9 Monitoring of Pesticide Residues from Vine to Wine

Monitoring of Pesticide Residues from Vine to Wine

Abstract

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines. Although no Maximum Residue Limits (MRLs) are set so far for the wine, powerful analysis methods have to be developed in order to detect low levels of compounds and prohibited molecules in particular. Moreover, organic winegrowers are concerned about contamination problems that may arise in the field or during the winemaking. In this work, a liquid chromatography – tandem mass spectrometry (LC-MS/MS) method is used to monitor about 190 pesticide residues. The extraction step is carried out either by QuEChERS (Quick, Easy, Cheap, Efficient, Rugged and Safe) or by SPE (Solid-Phase Extraction) for liquid samples. Optimisations were performed for the extraction of analytes from vine leaves (grinding, QuEChERS extraction and purification step) and validation parameters were controlled for different matrices (vine leaves, grapes, wines, lees) in terms of precision and accuracy. Several experiments were then conducted to monitor pesticide residues. For two vintages, vine leaves were analysed during the summer for a conventional plot as well as for an organic plot next to it. Sampling was done before and 48 hours after each phytosanitary treatment to evaluate contamination of the organic plot and the persistence of residues over time. Grapes, musts and wines were also analysed and when possible compared to the applied phytosanitary scheme to evaluate the persistence of compounds used in the vineyard. Sampling was also carried out all along the vinification process, from ripe berries to the wine during ageing in order to observe on one hand if contamination phenomena took place and eventually detect critical steps and on the other hand to estimate the transfer of active substances all along the process. Preliminary experiments were also conducted on the effect of alcoholic fermentation on pesticide residues at laboratory scale. Persistence and transfer are variable depending on molecules.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Céline Franc*, Gilles de Revel

*Université de Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

Attractiveness and sweetness of red wines: Synergies between American oak barrels and mannoproteins

In partnership with a Bordeaux property wanting to improve the quality of its second wine, the effects of two factors, American oak barrels and mannoproteins were studied. Their impact on the attractiveness and sweetness of wines were characterized during two successive vintages (2012 and 2013). Vinification took place with a homogeneous batch of Cabernet Sauvignon. The wine was then divided up into various groups of five barrels of French and American oak, new or reused. Analyses of volatile and non-volatile wood compounds were undertaken at four months and eight months of wood ageing, by LC-MS and GC-MS.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Screening sensory-directed methodology for the selection of non-saccharomyces wine yeasts based on perceived aroma quality

The present work contributes by developing a rapid sensory-directed methodology for the screening and selection of high quality wines with different sensory profiles Therefore, Verdejo and Tempranillo musts were fermented with 50 different yeasts each under controlled laboratory conditions. Resulting samples were firstly categorized according to five levels of quality by a panel of wine professionals (Sáenz-Navajas, Ballester et al. 2013). Higher quality samples were described by flash profiling by a semi-trained panel
(Valentin, Chollet et al. 2012) and most distinctive samples were screened by gas chromatography-olfactometry (GC-O) (López, Aznar et al. 2002).

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.