Macrowine 2021
IVES 9 IVES Conference Series 9 Monitoring of Pesticide Residues from Vine to Wine

Monitoring of Pesticide Residues from Vine to Wine

Abstract

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines. Although no Maximum Residue Limits (MRLs) are set so far for the wine, powerful analysis methods have to be developed in order to detect low levels of compounds and prohibited molecules in particular. Moreover, organic winegrowers are concerned about contamination problems that may arise in the field or during the winemaking. In this work, a liquid chromatography – tandem mass spectrometry (LC-MS/MS) method is used to monitor about 190 pesticide residues. The extraction step is carried out either by QuEChERS (Quick, Easy, Cheap, Efficient, Rugged and Safe) or by SPE (Solid-Phase Extraction) for liquid samples. Optimisations were performed for the extraction of analytes from vine leaves (grinding, QuEChERS extraction and purification step) and validation parameters were controlled for different matrices (vine leaves, grapes, wines, lees) in terms of precision and accuracy. Several experiments were then conducted to monitor pesticide residues. For two vintages, vine leaves were analysed during the summer for a conventional plot as well as for an organic plot next to it. Sampling was done before and 48 hours after each phytosanitary treatment to evaluate contamination of the organic plot and the persistence of residues over time. Grapes, musts and wines were also analysed and when possible compared to the applied phytosanitary scheme to evaluate the persistence of compounds used in the vineyard. Sampling was also carried out all along the vinification process, from ripe berries to the wine during ageing in order to observe on one hand if contamination phenomena took place and eventually detect critical steps and on the other hand to estimate the transfer of active substances all along the process. Preliminary experiments were also conducted on the effect of alcoholic fermentation on pesticide residues at laboratory scale. Persistence and transfer are variable depending on molecules.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Céline Franc*, Gilles de Revel

*Université de Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Mean polymerization degree of proanthocyanidins of grape seeds, skins and wines from Agiorgitiko (cv. Vitis vinifera): Differences among vintages

Grape phenolic compounds are very important constituents of red wine because, in addition to their antioxidant properties, they contribute to color, astringency and bitterness, oxidation reactions, interactions with proteins and ageing behavior of wines. The aim of our study was to assess the structural characteristics of grape and wine proanthocyanidins of Agiorgitiko variety and to evaluate the influence of the vintage year. Twelve vineyard locations were designated in the Nemea wine region. For three consecutive years (2012-2014), the grapes were harvested at technological maturity and the method of phloroglucinolysis was employed to determine the mean degree of polymerization (mDP) and subunit composition of the samples.

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”.

Characterization of commercial enological tannins and its effect on human saliva diffusion

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals.

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines.