Macrowine 2021
IVES 9 IVES Conference Series 9 Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Abstract

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues. The suitable feature of subcritical water leaching agent is its capacity to decrease dielectric constant as a function of increase in temperature, allowing a better solubility of the compounds of interest. In our study subcritical water extraction of polyphenols from red and white grape pomace from Dunkelfelder, Cabernet Franc, Merlot, and Chardonnay was performed. In semi-continuous extraction lead to crude extracts rich in different families of polyphenols. A purification step prior to industrial usage is essential. Coupling subcritical water extraction with membrane processes, due to large array of flexibility, offers a solution for the purification and fractioning of the crude extracts. The combined effects of extraction temperature (from 60 to 200 °C), pressure (from 25 to 100 bar), flow rate (1 to 10 ml/min), sample mass (5, 70) were investigated and compared to traditional solvent extraction (1/1 ethanol/water). Optimal extraction conditions were found to be 150 C and 6ml/min irrelevant of the pressure used. These conditions produced crude extracts containing 130 mg/100g DW of anthocyanins (+61% compared to traditional methods of extraction) and 2077mg/100g DW procyanidins (+23%). Subsequently to realize the purification of the crude extract, several organic membranes having differential molecular weight cut off 0.45 μm up to 200 Da were tested. The results allow evaluating both the permeate flux through the membranes and the rejection rates of the major compounds found in the crude extract. The tested membranes have differential selectivity for polysacharrides, proteins, and different families phenolic compounds (pentamers, trimers, anthocyanin, and phenolic acids) with high purity (95%). Confirming the applicability of membrane separation for the fractionation and purification of pomace extracts. More research is needed to validate the industrial up scaling and the intended application of the produced extracts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sami Yammine*, Martine Mietton-Peuchot, Remy Ghidossi, Robin Rabagliato, Xavier Vitrac

*University of Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Metabolomic profile of red non-V. vinifera genotypes

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera.

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure.