Macrowine 2021
IVES 9 IVES Conference Series 9 Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Abstract

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues. The suitable feature of subcritical water leaching agent is its capacity to decrease dielectric constant as a function of increase in temperature, allowing a better solubility of the compounds of interest. In our study subcritical water extraction of polyphenols from red and white grape pomace from Dunkelfelder, Cabernet Franc, Merlot, and Chardonnay was performed. In semi-continuous extraction lead to crude extracts rich in different families of polyphenols. A purification step prior to industrial usage is essential. Coupling subcritical water extraction with membrane processes, due to large array of flexibility, offers a solution for the purification and fractioning of the crude extracts. The combined effects of extraction temperature (from 60 to 200 °C), pressure (from 25 to 100 bar), flow rate (1 to 10 ml/min), sample mass (5, 70) were investigated and compared to traditional solvent extraction (1/1 ethanol/water). Optimal extraction conditions were found to be 150 C and 6ml/min irrelevant of the pressure used. These conditions produced crude extracts containing 130 mg/100g DW of anthocyanins (+61% compared to traditional methods of extraction) and 2077mg/100g DW procyanidins (+23%). Subsequently to realize the purification of the crude extract, several organic membranes having differential molecular weight cut off 0.45 μm up to 200 Da were tested. The results allow evaluating both the permeate flux through the membranes and the rejection rates of the major compounds found in the crude extract. The tested membranes have differential selectivity for polysacharrides, proteins, and different families phenolic compounds (pentamers, trimers, anthocyanin, and phenolic acids) with high purity (95%). Confirming the applicability of membrane separation for the fractionation and purification of pomace extracts. More research is needed to validate the industrial up scaling and the intended application of the produced extracts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sami Yammine*, Martine Mietton-Peuchot, Remy Ghidossi, Robin Rabagliato, Xavier Vitrac

*University of Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Oenological features of Sangiovese wine from vinification of whole grape berries

The present study was performed in a traditional winery located in the viticultural area of Brunello di Montalcino, Siena, Italy, in the vintage 2015. Actually, in this winery Sangiovese grape musts are fermented in large oak barrels by a single strain of Saccharomyces cerevisiae previously isolated in the same winery. Pumping over operations are carried out once or twice a day until the end of alcoholic fermentations. The aim of this work was to investigate on the oenological properties of Sangiovese wine produced with the traditional winemaking process adopted by the winery under study obtained from the fermentation of whole berries compared to that from crushed grape must. In particular, two lots of 65q of Sangiovese grapes from the same 3ha vineyard were vinified in 150hL oak barrels.

Development of a new sustainable filtering media for wine and beer clarification and sterilisation

Different separation techniques are frequently used during vinification process. Nowadays, clarification and microbiological stabilization of wine or beer can be done using precoat filters or crossflow filters to remove yeast and bacteria. Kieselguhr powders are the most used filter aids for precoat filtration. Their crystalline structure and their pulverulent nature induce ecotoxicological risks when used. Moreover, regeneration and reuse of these filter aids is not efficient and the filtration waste requires cost effective retreatment.

Comparison of aroma-related compounds of carbonic maceration and traditional young red winemaking in case of Merlot by means of targeted metabolomic approach

Winemaking decisions and techniques are known to affect the final aromatic composition of red wines. Winemakers put a constant effort into the improved controlling of vinification procedures to achieve better quality. Anyway an increased customer’s demand for uniqueness is often forcing them to adjust and offer new and new interesting products. To support the producers, an improved knowledge on aromatic potential as affected by classical and alternative strategies is needed.

Oxygen consumption by diferent oenological tanins in a model wine solution

INTRODUCTION: Oenological tannins are widely used in winemaking to improve some characteristics of wines [1] being the antioxidant properties probably one of the main reasons [2]. However, commercial tannins have different botanical sources and chemical composition [3] which probably determines different antioxidant potential. There are some few references about the antioxidant properties of commercial tannins [4] but none of them have really measured the direct oxygen consumption by them. The aim of this work was to measure the kinetics of oxygen consumption by different commercial tannins in order to determine their real capacities to protect wine against oxygen. MATERIAL AND METHODS: 4 different commercial tannins were used: T1: condensed tannin from grape seeds, T2: gallotannin from chinese gallnuts, T3: ellagitannin from oak and T4: tannin from quebracho containing condensed tannins and ellagitannins.

Application of high power ultrasounds during red wine vinification

Wine color is one of the main organoleptic characteristics influencing its quality. It is of especial interest in red vinifications due to the economic resources that wineries have to invest for the extraction of the phenolic compounds responsible of wine color, compounds that are mainly located inside the skin cell vacuoles. Moreover, these phenolic compounds not only influence color but also other organoleptic properties such as body, mouthfeel, astringency and flavour. The transference of phenolic compounds from grapes to must during vinification is closely related with the type of grapes and the winemaking technique.