Macrowine 2021
IVES 9 IVES Conference Series 9 Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Abstract

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues. The suitable feature of subcritical water leaching agent is its capacity to decrease dielectric constant as a function of increase in temperature, allowing a better solubility of the compounds of interest. In our study subcritical water extraction of polyphenols from red and white grape pomace from Dunkelfelder, Cabernet Franc, Merlot, and Chardonnay was performed. In semi-continuous extraction lead to crude extracts rich in different families of polyphenols. A purification step prior to industrial usage is essential. Coupling subcritical water extraction with membrane processes, due to large array of flexibility, offers a solution for the purification and fractioning of the crude extracts. The combined effects of extraction temperature (from 60 to 200 °C), pressure (from 25 to 100 bar), flow rate (1 to 10 ml/min), sample mass (5, 70) were investigated and compared to traditional solvent extraction (1/1 ethanol/water). Optimal extraction conditions were found to be 150 C and 6ml/min irrelevant of the pressure used. These conditions produced crude extracts containing 130 mg/100g DW of anthocyanins (+61% compared to traditional methods of extraction) and 2077mg/100g DW procyanidins (+23%). Subsequently to realize the purification of the crude extract, several organic membranes having differential molecular weight cut off 0.45 μm up to 200 Da were tested. The results allow evaluating both the permeate flux through the membranes and the rejection rates of the major compounds found in the crude extract. The tested membranes have differential selectivity for polysacharrides, proteins, and different families phenolic compounds (pentamers, trimers, anthocyanin, and phenolic acids) with high purity (95%). Confirming the applicability of membrane separation for the fractionation and purification of pomace extracts. More research is needed to validate the industrial up scaling and the intended application of the produced extracts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sami Yammine*, Martine Mietton-Peuchot, Remy Ghidossi, Robin Rabagliato, Xavier Vitrac

*University of Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization.

Characterization of Glycosidically Bound Aroma Compounds of País cv. grapes of different Chilean zones

País grape has been estimated to arrive to Chile almost 500 years ago, being the first strain grown in this country. Traditionally, this grape has been used to mix with other varieties, to produce poor quality wines, but today is beginning to be used in the production of high quality wines. However, very little is known about the chemical characteristics of this variety. The aroma is one of the most important quality attributes of wine. Volatile compounds of this beverage may come from the grape (varietal aromas), from the fermentation process, from the ageing. The aromatic compounds are found in the grape in two forms: as free volatile compounds and as non-volatile compounds. The last ones, are aroma precursors present mainly as glycoconjugates formed by a sugar and an aglycone…

Metabolomic profile of red non-V. vinifera genotypes

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera.

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.

Trans-resveratrol concentrations in wines Cabernet Sauvignon from Chile

This study evaluated the levels of trans-resveratrol in commercial wines made from Cabernet Sauvignon grapes from different valleys of Chile stilbenes. The Cabernet Sauvignon is the most planted variety in Chile, being 38% of the total vineyard country. Chile is the fourth largest wine exporter in the world, so it is important to evaluate the Cabernet-Sauvignon wines in their concentration levels of trans-resveratrol and its relation to the benefits provided to human health in moderate consumption. Evaluation comprises commercial wines from different valleys of Chile and its relationship with climatic characteristics, soil and vineyard handling.