Macrowine 2021
IVES 9 IVES Conference Series 9 Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Abstract

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues. The suitable feature of subcritical water leaching agent is its capacity to decrease dielectric constant as a function of increase in temperature, allowing a better solubility of the compounds of interest. In our study subcritical water extraction of polyphenols from red and white grape pomace from Dunkelfelder, Cabernet Franc, Merlot, and Chardonnay was performed. In semi-continuous extraction lead to crude extracts rich in different families of polyphenols. A purification step prior to industrial usage is essential. Coupling subcritical water extraction with membrane processes, due to large array of flexibility, offers a solution for the purification and fractioning of the crude extracts. The combined effects of extraction temperature (from 60 to 200 °C), pressure (from 25 to 100 bar), flow rate (1 to 10 ml/min), sample mass (5, 70) were investigated and compared to traditional solvent extraction (1/1 ethanol/water). Optimal extraction conditions were found to be 150 C and 6ml/min irrelevant of the pressure used. These conditions produced crude extracts containing 130 mg/100g DW of anthocyanins (+61% compared to traditional methods of extraction) and 2077mg/100g DW procyanidins (+23%). Subsequently to realize the purification of the crude extract, several organic membranes having differential molecular weight cut off 0.45 μm up to 200 Da were tested. The results allow evaluating both the permeate flux through the membranes and the rejection rates of the major compounds found in the crude extract. The tested membranes have differential selectivity for polysacharrides, proteins, and different families phenolic compounds (pentamers, trimers, anthocyanin, and phenolic acids) with high purity (95%). Confirming the applicability of membrane separation for the fractionation and purification of pomace extracts. More research is needed to validate the industrial up scaling and the intended application of the produced extracts.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Sami Yammine*, Martine Mietton-Peuchot, Remy Ghidossi, Robin Rabagliato, Xavier Vitrac

*University of Bordeaux

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Pesticide removal in wine with a physical treatment by molecular sieving

All along the winemaking process, conditioning and aging, wine is susceptible to be contaminated by different molecules. Contaminations can have various origins, related to wine microorganisms or as a result of an exogenous contamination. The aforementioned contamination of the wine can be caused by the migration of molecules from the materials in contact with the wine or by a contamination from exogenous molecules present in the air. Regardless of the source of the contamination, mainly two types of consequences can be observed.

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.

Anti/prooxidant activity of wine polyphenols in reactions of adrenaline auto-oxidation

Adrenaline (epinephrine) belongs to catecholamine class. It is a neurotransmitter and both a hormone which is released by the sympathetic nervous system and adrenal medulla in response to a range of stresses in order to regulate blood pressure, cardiac stimulation, relaxation of smooth muscles and other physiological processes. Adrenaline exhibits an effective antioxidant capacity (1). However, adrenalin is capable to auto-oxidation and in this case it generates toxic reactive oxygen intermediates and adrenochrome. Under in vitro conditions, auto-oxidation of adrenaline occurs in an alkaline medium (2).

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

Elicitors used as a tool to increase stilbenes in grapes and wines

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001).