Macrowine 2021
IVES 9 IVES Conference Series 9 Impact of elemental sulfur (S0) residues in Sauvignon blanc juice on the formation of the varietal thiols 3-mercapto hexanol and 3-mercaptohexyl acetate

Impact of elemental sulfur (S0) residues in Sauvignon blanc juice on the formation of the varietal thiols 3-mercapto hexanol and 3-mercaptohexyl acetate

Abstract

Elemental sulfur is a fungicide used by grape growers to control the development of powdery mildew, caused by the fungus Erysiphe necator. This compound is effective, cheap and has a low toxicity with no withholding period recommended. However, high levels of S0 residues in the harvested grapes can lead to the formation of reductive sulfur compounds that can impart taints and faults to the wine. Hydrogen sulphide (H2S) is a very volatile and unpleasant sulfur compound which formation is connected to high residues of S0 in juice (10 – 100 mg/L). These residues can be minimized with pressing and clarification of the juice prior to fermentation, but may increase during prolonged maceration. At the same time, H2S can play a role on the formation of the important varietal thiols 3-mercapto hexanol (3MH) and 4-mercapto-4-methylpentan-2-one (4MMP) as the direct sulfur donor to E-2-hexenal or mesityl oxide, respectively. Sauvignon blanc juices from three different locations was obtained at a commercial winery in Marlborough, New Zealand. One sample (A) was collected from the receival bin and pressed to obtain 25 L of juice. Two other samples (B and C) were collected from the commercial pressing operation. The samples were cold settled, racked to glass bottles (700 mL of juice), and then 0, 2, 10 or 50 mg/L of a wettable elemental sulfur compound was added. The fermentation was carried out using Saccharomyces cerevisiae (EC1118) at 15°C. The juices showed quite different potential to produce 3MH and 3MHA, and without any added sulfur, juice A produced a high amount of 3MH (6,000 ng/L), while juices B and C showed signs of oxidation and little 3MH was formed (< 600 ng/L). The addition of 50 mg/L of elemental sulfur caused a 1.7-fold increment in 3MH for juice A. For juice B detectable levels of 3MH and 3MHA were only observed for the extreme addition of 50 mg/L S0, which led to a 20-fold increase in 3MH production for juice C. Even though the results showed a clear relation between S0 in juice and varietal thiols in wine, the deliberate increase in the fungicide use close to harvest needs to be carefully managed, as levels of unwanted reductive sulfur compounds including H2S, methanethiol and carbon disulfide in the final wine were found to increase with the higher elemental sulfur additions.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Leandro Dias Araujo*, Bruno Fedrizzi, Paul Kilmartin, Suzanne Callerot, Wessel du Toit

*University of Auckland

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol).

The effect of Nitrogen and Sulphur foliar applications in hot climates

ine nitrogen deficiency can negatively influence the aroma profile and ageing potential of white wines. Canopy management can alter vine microclimate, affect the nitrogen availability and influence the response of leaf senescence. Increasing the nitrogen availability to vines can increase the Yeast Assimilable Nitrogen (YAN) levels in harvested fruit and wine. Studies show that foliar nitrogen and sulphur applications at véraison, on low YAN Sauvignon blanc grapes have an effect on the level of amino acids (Jreij et al. 2009) and on S-containing compounds such as glutathione and thiols (Lacroux et al. 2008), which in turn can influence the formation of major volatiles and the aroma profile of the wine.

Anthropogenic factors in modulations of fungal populations from grapes to wines and their repercussions on wine characteristics

The effects of anthropogenic activities on vineyard (different plant protections) and in winery
(pressing/clarification step, addition of sulfur dioxide) on fungal populations from grape to wine were studied. The studied anthropogenic activities modify the fungal diversity. Thus, lower biodiversity of grapes from organic modality was measured for the three vintages considered compared to biodiversity from ecophyto modality and conventional modality. The pressing / clarification steps strongly modify fungal populations and the influence of the winery flora is highlighted.

Supramolecular approaches to the study of the astringency elicited by wine phenolic compounds

The objective of this study is to review the scientific evidences and to advance into the knowledge of the molecular mechanisms of astringency. Astringency has been described as the drying, roughing and puckering sensation perceived when some food and beverages are tasted (1). The main, but possibly not the only, mechanism for the astringency is the precipitation of salivary proteins (2,3). Between phenolic compounds found in red wines, flavan-3-ols are the group usually related to the development of this sensation. Other compounds, phenolic or not, like anthocyanins, polysaccharides and mannoproteins could act modifying or modulating astringency perception by hindering the interaction between flavanols and salivary proteins either because of their interaction with the flavanols or because of their interaction with the salivary proteins.