Macrowine 2021
IVES 9 IVES Conference Series 9 Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

Abstract

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine. The aim of this study was to compare the protein composition of B. cinerea infected and healthy grapes as well as of wines produced from such grapes in regard to proteins which might play a potential role in the gushing phenomenon of sparkling wine. Therefore, SDS-PAGE and reversed phase HPLC (RP-HPLC) were applied to analyze the protein composition of healthy and botrytized Weißburgunder grapes and the corresponding wines. The fungal infection led to a general decrease of the protein content in infected grapes and wines suggesting a proteolytic activity of B. cinerea. Especially the concentration of a protein with a molecular mass of ~17 kDa underwent a significant reduction in wine from infected grapes as compared to wine made from healthy grapes. Amino acid sequence analysis showed that this protein derives from Vitis vinifera. Other proteins were detected via SDS-PAGE and were shown to occur in the botrytized but not in healthy wines. These unidentified proteins were assumed to be related to the fungal infection, either as induced plant proteins or as proteins produced by the pathogen. Similar results were found when the proteome of non-gushing and gushing sparkling wines were compared. The protein content in gushing sparkling wines was much lower as compared to non-gushing sparkling wines when analyzed by SDS-PAGE and RP-HPLC. Furthermore, in gushing sparkling wine proteins of fungal origin can be found, whereas plant-associated pathogenesis related proteins were enriched in the non-gushing samples. The before mentioned protein (MW: ~17 kDa) was absent or at least reduced in gushing sparkling wine samples. Hence, an infection with B. cinerea led to several proteomic changes in grapes, which were still detectable in the wine made thereof. Degradation of plant proteins and occurrence of fungal proteins were also observed in gushing sparkling wines. These parallels suggest that gushing in sparkling wine might be affected by a degradation of proteins induced by an infection with fungal pathogens.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ludwig Niessen*, Elisabeth Vogt, Rudi Vogel, Tobias Ziegler, Veronika Kupfer

*TU München

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.

Improving the phenolic composition of cv tempranillo wines by blending grapes of different ripening state

The aim of this work was to reduce the alcohol content of Tempranillo wine. Tempranillo wines were produced by grapes harvested at different ripening dates (August 11 which was 21 oBrix and September 28 with 25 oBrix). At the second date, the Tempranillo wines were elaborated as follows: grapes were destemmed, crushed and collected into 50 L stainless-steel vats. Before preferementative maceration in cold, 50 % (M1) and 70 % (M2) of the must have been replaced by the same percentage of must from the first harvest. In addition, a control wine (C) was performed with only grapes from the second harvest.

Non-invasive headspace sorptive extraction for monitoring volatile compounds production by saccharomyces and non-saccharomyces strains throughout alcoholic fermentation

Wine is a solution containing abundant volatile compounds which contribute to their aroma. Many of them are produced by yeast as metabolism by-products. Different yeast strains produce different volatile profiles. The possibility of studying the evolution of volatile compounds during fermentation, using sampling methods that not alter the volume of fermentation media, is of great interest. In spite of this, non-invasive methods to monitoring the evolution of volatile profile during fermentation have been seldom used. The goals of this work were to use by first time the headspace sorptive extraction (HSSE) as non-invasive method to monitor the evolution of volatile profiles throughout alcoholic fermentation and to study the changes on volatile profiles produced by Saccharomyces cerevisiae and Lachancea thermotolerans during fermentation of a must with high sugar content.

Characterization of non-Saccharomyces yeast and its interaction with Saccharomyces cerevisiae with investigation of fermentation kinetics and aromatic composition

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.20.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.