Macrowine 2021
IVES 9 IVES Conference Series 9 Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

Abstract

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine. The aim of this study was to compare the protein composition of B. cinerea infected and healthy grapes as well as of wines produced from such grapes in regard to proteins which might play a potential role in the gushing phenomenon of sparkling wine. Therefore, SDS-PAGE and reversed phase HPLC (RP-HPLC) were applied to analyze the protein composition of healthy and botrytized Weißburgunder grapes and the corresponding wines. The fungal infection led to a general decrease of the protein content in infected grapes and wines suggesting a proteolytic activity of B. cinerea. Especially the concentration of a protein with a molecular mass of ~17 kDa underwent a significant reduction in wine from infected grapes as compared to wine made from healthy grapes. Amino acid sequence analysis showed that this protein derives from Vitis vinifera. Other proteins were detected via SDS-PAGE and were shown to occur in the botrytized but not in healthy wines. These unidentified proteins were assumed to be related to the fungal infection, either as induced plant proteins or as proteins produced by the pathogen. Similar results were found when the proteome of non-gushing and gushing sparkling wines were compared. The protein content in gushing sparkling wines was much lower as compared to non-gushing sparkling wines when analyzed by SDS-PAGE and RP-HPLC. Furthermore, in gushing sparkling wine proteins of fungal origin can be found, whereas plant-associated pathogenesis related proteins were enriched in the non-gushing samples. The before mentioned protein (MW: ~17 kDa) was absent or at least reduced in gushing sparkling wine samples. Hence, an infection with B. cinerea led to several proteomic changes in grapes, which were still detectable in the wine made thereof. Degradation of plant proteins and occurrence of fungal proteins were also observed in gushing sparkling wines. These parallels suggest that gushing in sparkling wine might be affected by a degradation of proteins induced by an infection with fungal pathogens.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ludwig Niessen*, Elisabeth Vogt, Rudi Vogel, Tobias Ziegler, Veronika Kupfer

*TU München

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Influence of inactive dry yeast treatments during grape ripening on postharvest berry skin texture parameters and phenolic compounds extractability

Inactive dry yeast treatments in the vineyard are a tool used with the aim to improve the concentration and quality of secondary metabolites in grapes, leading to a better differentiation of the wines made from grapes differently treated. In this work, a foliar spraying treatment with yeast derivatives specifically designed to be used with the patent pending application technology of Lallemand Inc. Canada (LalVigne® Mature, Lallemand Inc., Montreal, Canada) was tested on Vitis vinifera L. cv. Barbera and Nebbiolo black winegrapes. The aim was to evaluate the effect of this treatment on the phenolic compounds accumulation, the skin physical-mechanical properties and the related phenolic extractability. Prior to analysis, the berries were sorted by flotation in order to evaluate their distribution by density class, and to determine the skin texture parameters of berries with different sugar contents, thus understanding also the ripening effect.

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.

Interactions of wine polyphenols with dead or living Saccharomyces cerevisiae Yeast Cells and Cell Walls: polyphenol location by microscopy

Tannin, anthocyanins and their reaction products play a major role in the quality of red wines. They contribute to their sensory characteristics, particularly colour and astringency. Grape tannins and anthocyanins are extracted during red wine fermentation. However, their concentration and composition change over time, due to their strong chemical reactivity1. It is also well known that yeasts influence the wine phenolic content, either through the release of metabolites involved in the formation of derived pigments1, or through polyphenol adsorption2,3.

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide.