Macrowine 2021
IVES 9 IVES Conference Series 9 Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

Abstract

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine. The aim of this study was to compare the protein composition of B. cinerea infected and healthy grapes as well as of wines produced from such grapes in regard to proteins which might play a potential role in the gushing phenomenon of sparkling wine. Therefore, SDS-PAGE and reversed phase HPLC (RP-HPLC) were applied to analyze the protein composition of healthy and botrytized Weißburgunder grapes and the corresponding wines. The fungal infection led to a general decrease of the protein content in infected grapes and wines suggesting a proteolytic activity of B. cinerea. Especially the concentration of a protein with a molecular mass of ~17 kDa underwent a significant reduction in wine from infected grapes as compared to wine made from healthy grapes. Amino acid sequence analysis showed that this protein derives from Vitis vinifera. Other proteins were detected via SDS-PAGE and were shown to occur in the botrytized but not in healthy wines. These unidentified proteins were assumed to be related to the fungal infection, either as induced plant proteins or as proteins produced by the pathogen. Similar results were found when the proteome of non-gushing and gushing sparkling wines were compared. The protein content in gushing sparkling wines was much lower as compared to non-gushing sparkling wines when analyzed by SDS-PAGE and RP-HPLC. Furthermore, in gushing sparkling wine proteins of fungal origin can be found, whereas plant-associated pathogenesis related proteins were enriched in the non-gushing samples. The before mentioned protein (MW: ~17 kDa) was absent or at least reduced in gushing sparkling wine samples. Hence, an infection with B. cinerea led to several proteomic changes in grapes, which were still detectable in the wine made thereof. Degradation of plant proteins and occurrence of fungal proteins were also observed in gushing sparkling wines. These parallels suggest that gushing in sparkling wine might be affected by a degradation of proteins induced by an infection with fungal pathogens.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ludwig Niessen*, Elisabeth Vogt, Rudi Vogel, Tobias Ziegler, Veronika Kupfer

*TU München

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.

To a better understanding of the impact of vine nitrogen status on volatile thiols from plot to transcriptome level

Volatile thiols contribute largely to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless and non-volatile precursors found in the berry and the must. The effect of vine nitrogen status on 3SH and 4MSP in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) was investigated in this study.

Cytochrome P450 CYP71BE5 from grapevine (Vitis vinifera) catalyzes the formation of the spicy aroma compound, (-)-rotundone

(-)-Rotundone, an oxygenated sesquiterpene, is a potent odorant molecule with a characteristic spicy aroma existing in various plants including grapes1. It is considered as a significant compound notably in wines and grapes because of its low sensory threshold (16 ng L-1 in red wine, 8 ng L-1 in water) and aroma properties. (-)-Rotundone was first identified in red wine made from the grape cultivar Syrah (regionally called Shiraz) in Australia1, and then it was found in several grape varieties such as Duras, Grüner Veltliner, Schioppettino and Vespolina from Europe2, 3. Several environmental factors affecting the accumulation of (-)-Rotundone during the grape maturation, were reported such as ambient temperature4, soil properties and topography5, soil moisture from irrigation and light exposure in the bunch zone by leaf removal2.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Effect of mixed Torulaspora delbrueckii-Saccharomyces cerevisiae culture on rose quality wine

Alcoholic fermentation using no Saccharomyces wine is an effective means of modulating wine aroma. This study investigated the impact of coinoculating Torulaspora delbruecki with two Saccharomyces cerevisiae commercial yeast (QA23, Lallemand; Red Fruit, Sepsa-Enartis) on enological quality parameters, volatile composition and sensory analysis. The following assays were performed on Tempranillo variety: Saccharomyces QA23 (CTQA), Saccharomyces Red Fruit (CTRF), coinoculated T. delbrueckii + S.cerevisiae QA23 (CIQA) and coinoculated T. delbrueckii + S.cerevisiae (CIRF).