Metabolomics of grape polyphenols as a consequence of post-harvest drying: on-plant dehydration vs warehouse withering
Abstract
A method of suspect screening analysis to study grape metabolomics, was developed [1]. By performing ultra-high performance liquid chromatography (UHPLC) – high-resolution mass spectrometry (HRMS) analysis of the grape extract, averaging 320-450 putative grape compounds are identified which include mainly polyphenols. Identification of metabolites is performed by a new HRMS-database of putative grape and wine compounds expressly constructed (GrapeMetabolomics) which currently includes around 1,100 entries. Grape dehydration is an oenological process used in the production of a number of non-botrytized sweet and not-sweet Italian wines: e.g., Amarone di Valpolicella (produced by Corvina, Corvinone and Rondinella grapes), Passito di Pantelleria (Zibibbo grape), VinSanto (Malvasia and Trebbiano grapes), Sfursat (Nebbiolo grape), Raboso Passito. The process is carried out by keeping grape on-vine for a certain period of time after cutting the yield cane (up to two/three months), or by leaving the grape in dehydration warehouses under controlled conditions of humidity and temperature [2-6]. Metabolomics of polyphenols of Corvina grape dehydrated both in-plant and warehouse withering was studied by performing UHPLC-QTOF analysis of grape extracts. In particular, the study was focalized on the principal classes of polyphenolic compounds of grape, such as anthocyanins, flavonols and stilbene derivatives [7,8]. Differences between the two dehydration methods were evaluated by statistical analysis.
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Issue: Macrowine 2016
Type: Poster
Authors
*CREA