Macrowine 2021
IVES 9 IVES Conference Series 9 Quantification of red wine phenolics using ultraviolet-visible, near and mid-infrared spectroscopy combined with chemometrics

Quantification of red wine phenolics using ultraviolet-visible, near and mid-infrared spectroscopy combined with chemometrics

Abstract

The use of multivariate statistics to correlate chemical data to spectral information seems as a valid alternative for the quantification of red wine phenolics. The advantages of these techniques include simplicity and cost effectiveness together with the limited time of analysis required. Although many publications on this subject are nowadays available in the literature most of them only reported feasibility studies. In this study 400 samples from thirteen fermentations including five different cultivars plus 150 wine samples from a varying number of vintages were submitted to spectrophotometric and chromatographic phenolic analysis. Anthocyanins, total phenolics, tannins, colour density and the most representative compounds within the main phenolic families (hydroxicinnamic acids, flavan-3-ols, flavonols and anthocyanins) were quantified. Spectra were recorded in different regions of the electromagnetic spectrum. Particularly the information contained in the ultraviolet-visible region as well as in the near and mid-infrared regions was collected. Regression models were built and validated. The interpretation of the loadings and coefficients of regression, the evaluation and analysis of the correlation among variables and the measured phenolic compounds as well as the chemistry basis behind each quantified compound was extensively investigated and reported. Spectral pre-processing techniques as well as variable selection tools were also investigated and selected based on model performance. Accurate models for most of the phenolic compounds and spectroscopies were obtained with residual predictive deviation (RPD) values higher than 2.5. The results obtained showed UV-visible and infrared spectroscopy as valid approaches for the quantification of the phenolic content throughout the winemaking process. Considerations such as easiness of use and the economical and human resources involved in the analysis will also be discussed.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Jose Luis Aleixandre-Tudo*, Helene Nieuwoudt, Wessel du Toit

*Stellenbosch University

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Metabolomic profile of red non-V. vinifera genotypes

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera.

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

Influence of preflowering basal leaf removal on aromatic composition of cv. Tempranillo wine from semiarid climate (Extremadura Western Spain)

Abstract In this work the effects of early leaf removal performed manually at preflowering phenological stage, on the volatile composition of Tempranillo (Vitis vinifera L.) wines were studied. From 2009-2011 vintages 34 wine volatile compounds were identified and quantified by gas chromatography-mass spectrometry (GC-MS) where early leaf removal only modified 25 of them. The total C6 compounds, acetates and volatiles acids (with exception of isobutyric acid) were affected by defoliation, whereas alcohols and esters showed a minor effect. Furthermore the vintage effect also was shown.

Novel contribution to the study of mouth-feel properties in wines

In general, there is a well-established lexicon related to wine aroma and taste properties; however mouth-feel-related vocabulary usually includes heterogeneous, multimodal and personalized terms. Gawel et al.
(2000) published a wheel related to mouthfeel properties of red wine. However, its use in scientific publications has been limited. The authors accepted that the approach had certain limitations as it included redundant and terms with hedonic tone and some others were absent. It is of high interest to generate a mouth-feel lexicon and finding the chemical compound or group of compounds responsible for such properties in red wine. In the present work a chemical fractionation method has been developed.

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.