Macrowine 2021
IVES 9 IVES Conference Series 9 Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

Abstract

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure. Therefore, the binding of ethyl esters to a wine protein has been analyzed by studying the modifications of the protein structure (which indicates protein-ligand interactions) by Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy (2). The effects induced by the addition of ethyl esters (from hexanoate to dodecanoate) on the secondary structure and stability of a purified Thaumatin like-protein (VVTL1), the most abundant wine protein, was studied in a wine model solution (12% ethanol, 5 g/l mesotartaric acid, pH 3.2). As demonstrated by UV-photo denaturation assays (20 repeated consecutive scans in the far UV-region of protein), the secondary structure of VVTL1 was only slightly affected by the presence of the selected aroma esters, but protein stability was increased by the addiction of octanoate, decanoate and dodecanoate ethyl esters. On the contrary, in the presence of ethyl hexanoate protein stability decreases. These data were further confirmed by SRCD thermal denaturation assay. The results here reported demonstrate that the content of ordered structure and the protein photo and thermal stability of the main wine protein VVTL1 is modified by ethyl esters of different chain length, indicating the existence of a binding phenomenon. Therefore esters interactions with proteins may occur in wine and that this fact can modulate both the effect of bentonite treatments and the perception of the wine aroma.

(1) Vincenzi et al., 2015. J. Agric. Food Chem., 63, 2314 (2) Hussain R. et al., 2012. Spectroscopic Analysis: Synchrotron Radiation Circular Dichroism, in: Comprehensive Chirality, 8, Elsevier, Amsterdam, pp. 438-448.

ACKNOWLEDGMENTS We thank Diamond Light Source for access to beamline B23 (SM8034) that contributed to the results presented here. This research has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement nº 226716.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Andrea Curioni*, Diana Gazzola, Mattia Di Gaspero, Paolo Ruzza, Simone Vincenzi

*Università di Padova

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Ageing of Sauvignon Blanc white wines with Specific Inactivated Dry Yeasts: Effect on physical and chemical characteristics

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Field-grown Sauvignon Blanc berries react to increased exposure by controlling antioxidant homeostasis and displaying UV acclimation responses that are influenced by the level of ambient light

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

The effect of Nitrogen and Sulphur foliar applications in hot climates

ine nitrogen deficiency can negatively influence the aroma profile and ageing potential of white wines. Canopy management can alter vine microclimate, affect the nitrogen availability and influence the response of leaf senescence. Increasing the nitrogen availability to vines can increase the Yeast Assimilable Nitrogen (YAN) levels in harvested fruit and wine. Studies show that foliar nitrogen and sulphur applications at véraison, on low YAN Sauvignon blanc grapes have an effect on the level of amino acids (Jreij et al. 2009) and on S-containing compounds such as glutathione and thiols (Lacroux et al. 2008), which in turn can influence the formation of major volatiles and the aroma profile of the wine.