Macrowine 2021
IVES 9 IVES Conference Series 9 Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

Abstract

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure. Therefore, the binding of ethyl esters to a wine protein has been analyzed by studying the modifications of the protein structure (which indicates protein-ligand interactions) by Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy (2). The effects induced by the addition of ethyl esters (from hexanoate to dodecanoate) on the secondary structure and stability of a purified Thaumatin like-protein (VVTL1), the most abundant wine protein, was studied in a wine model solution (12% ethanol, 5 g/l mesotartaric acid, pH 3.2). As demonstrated by UV-photo denaturation assays (20 repeated consecutive scans in the far UV-region of protein), the secondary structure of VVTL1 was only slightly affected by the presence of the selected aroma esters, but protein stability was increased by the addiction of octanoate, decanoate and dodecanoate ethyl esters. On the contrary, in the presence of ethyl hexanoate protein stability decreases. These data were further confirmed by SRCD thermal denaturation assay. The results here reported demonstrate that the content of ordered structure and the protein photo and thermal stability of the main wine protein VVTL1 is modified by ethyl esters of different chain length, indicating the existence of a binding phenomenon. Therefore esters interactions with proteins may occur in wine and that this fact can modulate both the effect of bentonite treatments and the perception of the wine aroma.

(1) Vincenzi et al., 2015. J. Agric. Food Chem., 63, 2314 (2) Hussain R. et al., 2012. Spectroscopic Analysis: Synchrotron Radiation Circular Dichroism, in: Comprehensive Chirality, 8, Elsevier, Amsterdam, pp. 438-448.

ACKNOWLEDGMENTS We thank Diamond Light Source for access to beamline B23 (SM8034) that contributed to the results presented here. This research has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement nº 226716.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Andrea Curioni*, Diana Gazzola, Mattia Di Gaspero, Paolo Ruzza, Simone Vincenzi

*Università di Padova

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze.

Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages

An alternative to improve grape quality is the application to the vineyard of elicitors. Although these compounds were first used to increase resistance of plants against pathogens, it has been found that they are also able to induce mechanisms involved in the synthesis of phenolic compounds and some amino acids. However, researches about the influence of elicitors on grape volatile composition are scarcely. Therefore, the aim of this work was to study the influence of methyl jasmonate (MeJ) foliar application on grape aroma composition over three consecutive vintages. MeJ was applied to Tempranillo grapevines at a concentration of 10 mM in 2013, 2014, and 2015 years. Control plants were sprayed with water.

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels.

Impact of industrial-scale serial filtration on macromolecules in red wines

Filtration is a critical step in ensuring the clarity and microbial stability of wine prior to bottling. However the process of filtering potentially reduces red wine quality by removing some of the macromolecules that contribute to the texture of the wine. Commercial red wines, Cabernet Sauvignon (CAS) and Shiraz (SHZ), of two vintages and two grades (premium grade wines from the older vintage: CAS13 and SHZ13; and standard grade wines from a younger vintage: CAS14 and SHZ14) were filtered through industrial-scale commercial filtration units prior to bottling. Samples were taken before and after cross-flow filtration, lenticular filters, 0.65 µm and 0.45 µm pore size nylon membrane filters. The concentration and composition of macromolecules, including tannins and polysaccharides, were measured in all samples as well as particle size distribution and wine colour.

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T