Macrowine 2021
IVES 9 IVES Conference Series 9 Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

Ethyl esters interact with the major wine Thaumatin Like Protein VVTL1

Abstract

The interactions among aromatic compounds and proteins is an important issue for the quality of foods and beverages. In wine, the loss of flavor after vinification is associated to bentonite treatment and this effect can be the result of the removal of aroma compounds which are bound wine proteins. This phenomenon was recently demonstrated for long chain fatty acids and their ethyl esters (1). Since these latter compounds are spectroscopically silent, their association with proteins is not easy to measure. Therefore, the binding of ethyl esters to a wine protein has been analyzed by studying the modifications of the protein structure (which indicates protein-ligand interactions) by Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy (2). The effects induced by the addition of ethyl esters (from hexanoate to dodecanoate) on the secondary structure and stability of a purified Thaumatin like-protein (VVTL1), the most abundant wine protein, was studied in a wine model solution (12% ethanol, 5 g/l mesotartaric acid, pH 3.2). As demonstrated by UV-photo denaturation assays (20 repeated consecutive scans in the far UV-region of protein), the secondary structure of VVTL1 was only slightly affected by the presence of the selected aroma esters, but protein stability was increased by the addiction of octanoate, decanoate and dodecanoate ethyl esters. On the contrary, in the presence of ethyl hexanoate protein stability decreases. These data were further confirmed by SRCD thermal denaturation assay. The results here reported demonstrate that the content of ordered structure and the protein photo and thermal stability of the main wine protein VVTL1 is modified by ethyl esters of different chain length, indicating the existence of a binding phenomenon. Therefore esters interactions with proteins may occur in wine and that this fact can modulate both the effect of bentonite treatments and the perception of the wine aroma.

(1) Vincenzi et al., 2015. J. Agric. Food Chem., 63, 2314 (2) Hussain R. et al., 2012. Spectroscopic Analysis: Synchrotron Radiation Circular Dichroism, in: Comprehensive Chirality, 8, Elsevier, Amsterdam, pp. 438-448.

ACKNOWLEDGMENTS We thank Diamond Light Source for access to beamline B23 (SM8034) that contributed to the results presented here. This research has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement nº 226716.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Andrea Curioni*, Diana Gazzola, Mattia Di Gaspero, Paolo Ruzza, Simone Vincenzi

*Università di Padova

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effects of bottle closure type on sensory characteristics of Chasselas wines

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.

Metabolomic profile of red non-V. vinifera genotypes

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).