Macrowine 2021
IVES 9 IVES Conference Series 9 Multivariate strategies for red wines classification using stilbenes and flavonols content

Multivariate strategies for red wines classification using stilbenes and flavonols content

Abstract

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C). During such method validation, the selectivity was confirmed by a high resolution mass spectrometer (QTOF). For statistical analysis, four different data sets were used: wine varieties (34 samples), process influence (58 samples), harvest years (54 samples) and micro-regions (58 samples). The analysis of variance (ANOVA), principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were used. The Kennard-Stone algorithm was used to separate the samples into training and test sets. The leave-one-out cross validation method was used to choose the number of latent variables (LVs) in PLS-DA. The limits of detection (LOD) and quantification (LOQ) of (R), (Q), (M), (K), (V) and (C) were, respectively, 0.33 and 1.01, 0.30 and 0.90, 0.23 and 0.69, 0.27 and 0.81, 0.23 and 0.70, 0.02 and 0.05 µg mL-1. The concentration of (C) was below the LOQ in all samples, since it is consumed to synthesize the studied SaF. According to ANOVA, the SaF concentrations changed significantly due to the influence of the studied parameters. However, no patterns were observed in the scores of the first three principal components (PCs) of the PCA for harvest year and micro-regions data set. A tendency of separation was observed in the PCA scores for different varieties and processing data set. Through PLS-DA, it was possible to satisfactorily predict the wine variety and the processing through the concentration of SaF in terms of sensitivity and specificity. These figures of merit were between 67-100% for both data sets. These results indicate that the concentration of secondary metabolites trans-resveratrol, quercetin, myricetin, kaempferol and trans-e-viniferin, determined by HPLC-DAD, have the potential to measure the variation of red wines between micro-regions from “Campanha Gaúcha”. Consequently, it may be part of an efficient strategy to elaborate different styles of wines adapted to a whole region.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Letícia Silva*, Ana Bergold, Celito Crivellaro Guerra, Marcelo Marcelo, Marco Ferrão

*Embrapa and UFRGS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Ageing of Sauvignon Blanc white wines with Specific Inactivated Dry Yeasts: Effect on physical and chemical characteristics

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Maturation of Agiorgitiko (Vitis vinifera) red wine on its wine lees: Impact on its phenolic composition

Maturation of wine on lees (often referred as sur lie) is a common practice applied by many winemakers around the world. In the past this method was applied mainly on white and/or sparkling wine production but recently also to red wine production. In our experiment, we matured red wine on wine lees of two origins: a) Light wine lees, collected after the completion of the alcoholic fermentation, b) Heavy lees, collected after the completion of the malolactic fermentation. The lees were free of off-odors and were added in the red wine in percentage 3% and 8%, simulating common winemaking addition. The maturation lasted in total six months and samples were collected for analysis after one, three and six months. During storage the lees were stirred.

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.

Oxygen consumption by diferent oenological tanins in a model wine solution

INTRODUCTION: Oenological tannins are widely used in winemaking to improve some characteristics of wines [1] being the antioxidant properties probably one of the main reasons [2]. However, commercial tannins have different botanical sources and chemical composition [3] which probably determines different antioxidant potential. There are some few references about the antioxidant properties of commercial tannins [4] but none of them have really measured the direct oxygen consumption by them. The aim of this work was to measure the kinetics of oxygen consumption by different commercial tannins in order to determine their real capacities to protect wine against oxygen. MATERIAL AND METHODS: 4 different commercial tannins were used: T1: condensed tannin from grape seeds, T2: gallotannin from chinese gallnuts, T3: ellagitannin from oak and T4: tannin from quebracho containing condensed tannins and ellagitannins.

Microbial life in the grapevine: what can we expect from the leaf microbiome?

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents.