Macrowine 2021
IVES 9 IVES Conference Series 9 Multivariate strategies for red wines classification using stilbenes and flavonols content

Multivariate strategies for red wines classification using stilbenes and flavonols content

Abstract

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C). During such method validation, the selectivity was confirmed by a high resolution mass spectrometer (QTOF). For statistical analysis, four different data sets were used: wine varieties (34 samples), process influence (58 samples), harvest years (54 samples) and micro-regions (58 samples). The analysis of variance (ANOVA), principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were used. The Kennard-Stone algorithm was used to separate the samples into training and test sets. The leave-one-out cross validation method was used to choose the number of latent variables (LVs) in PLS-DA. The limits of detection (LOD) and quantification (LOQ) of (R), (Q), (M), (K), (V) and (C) were, respectively, 0.33 and 1.01, 0.30 and 0.90, 0.23 and 0.69, 0.27 and 0.81, 0.23 and 0.70, 0.02 and 0.05 µg mL-1. The concentration of (C) was below the LOQ in all samples, since it is consumed to synthesize the studied SaF. According to ANOVA, the SaF concentrations changed significantly due to the influence of the studied parameters. However, no patterns were observed in the scores of the first three principal components (PCs) of the PCA for harvest year and micro-regions data set. A tendency of separation was observed in the PCA scores for different varieties and processing data set. Through PLS-DA, it was possible to satisfactorily predict the wine variety and the processing through the concentration of SaF in terms of sensitivity and specificity. These figures of merit were between 67-100% for both data sets. These results indicate that the concentration of secondary metabolites trans-resveratrol, quercetin, myricetin, kaempferol and trans-e-viniferin, determined by HPLC-DAD, have the potential to measure the variation of red wines between micro-regions from “Campanha Gaúcha”. Consequently, it may be part of an efficient strategy to elaborate different styles of wines adapted to a whole region.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Letícia Silva*, Ana Bergold, Celito Crivellaro Guerra, Marcelo Marcelo, Marco Ferrão

*Embrapa and UFRGS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.

Directed Evolution of Oenococcus oeni: optimising yeast-bacteria interactions for improved malolactic fermentation

Malolactic fermentation (MLF) is a secondary step in the vinification process and it follows alcoholic fermentation (AF) which is predominantly carried out by Saccharomyces cerevisiae. These two processes result in the degradation of metabolites to produce secondary metabolites which also contribute to the final wine flavour and quality. AF results in the production of ethanol and carbon dioxide from sugars and MLF stems from the degradation of L-malic acid (a dicarboxylic acid) to L-lactic acid (a monocarboxylic acid). The latter process results in a smoother texture as the acidity of the wine is reduced by the process, it also adds to the flavour complexity of the wine.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Cover crops influence on soil N availability and grapevine N status, and its relationship with biogenic

The type of soil management, tillage versus cover crops, can modify the soil microbial activity, which causes the mineralization of organic N to NO3–N and, therefore, may change the soil NO3–N availability in vineyard. The soil NO3–N availability could influence the grapevine nutritional status and the grape amino acid composition. Amino acids are precursors of biogenic amines, compounds mainly formed during the malolactic fermentation. Biogenic amines have negative effects on consumer health and on the wine organoleptic quality. The objective was to study if the effect of conventional tillage and two different cover crops (leguminous versus gramineous) on grapevine N status, could relate to the wine biogenic amines composition.