Macrowine 2021
IVES 9 IVES Conference Series 9 Multivariate strategies for red wines classification using stilbenes and flavonols content

Multivariate strategies for red wines classification using stilbenes and flavonols content

Abstract

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C). During such method validation, the selectivity was confirmed by a high resolution mass spectrometer (QTOF). For statistical analysis, four different data sets were used: wine varieties (34 samples), process influence (58 samples), harvest years (54 samples) and micro-regions (58 samples). The analysis of variance (ANOVA), principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were used. The Kennard-Stone algorithm was used to separate the samples into training and test sets. The leave-one-out cross validation method was used to choose the number of latent variables (LVs) in PLS-DA. The limits of detection (LOD) and quantification (LOQ) of (R), (Q), (M), (K), (V) and (C) were, respectively, 0.33 and 1.01, 0.30 and 0.90, 0.23 and 0.69, 0.27 and 0.81, 0.23 and 0.70, 0.02 and 0.05 µg mL-1. The concentration of (C) was below the LOQ in all samples, since it is consumed to synthesize the studied SaF. According to ANOVA, the SaF concentrations changed significantly due to the influence of the studied parameters. However, no patterns were observed in the scores of the first three principal components (PCs) of the PCA for harvest year and micro-regions data set. A tendency of separation was observed in the PCA scores for different varieties and processing data set. Through PLS-DA, it was possible to satisfactorily predict the wine variety and the processing through the concentration of SaF in terms of sensitivity and specificity. These figures of merit were between 67-100% for both data sets. These results indicate that the concentration of secondary metabolites trans-resveratrol, quercetin, myricetin, kaempferol and trans-e-viniferin, determined by HPLC-DAD, have the potential to measure the variation of red wines between micro-regions from “Campanha Gaúcha”. Consequently, it may be part of an efficient strategy to elaborate different styles of wines adapted to a whole region.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Letícia Silva*, Ana Bergold, Celito Crivellaro Guerra, Marcelo Marcelo, Marco Ferrão

*Embrapa and UFRGS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The use of cation exchange resins for wine acidity adjustment: Optimization of the process and the effects on tartrate formation and oxidative stability

Acidity adjustments are key to microbial control, sensory quality and wine longevity. Acidification with cation exchange resins -in acid cycle- offers the possibility to reduce the pH by exchanging wine cations, such as potassium (K+), for hydrogen ions (H+). During the exchange process, the removal of potassium and calcium ions contributes to limiting the formation of tartrate salts, thus offering an alternative solution to conventional methods for tartrate stability. Moreover, the reduction of wine pH and the removal of metals catalyzers (e.g. iron) could positively impact the wine’s oxidative stability. Therefore, the aims of this work were (a) to optimize the ion exchange process by testing different volumes and concentrations of sulfuric acid (H2SO4) during the acid cycle, (b) evaluate the effects of the ion exchange process on the formation of tartrate salts, and (c) analyze the oxidative stability of the treated wines.

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”.

Effect of intra‐vineyard ripeness variation on the efficiency of commercial enzymes on berry cell wall deconstruction under winemaking conditions

Intra-vineyard variation grape berry ripening occurs within bunches, between bunches on the same vine and between vines. Although it is assumed that such variation also occurs at the grape berry cell wall level, no study to data has investigated in any depth. Here we have used a intra-vineyard panel design to investigate pooled bunches from six vines (per panel) in the context of a winemaking scenario. The dissected vineyard was harvested by separate panels, where each panel was then subjected to a standard winemaking procedure with or without the addition of three different enzyme preparations for maceration.

Light-struck taste in white wine: enological approach for its prevention

Light-struck taste is a defect prevalent in white wines bottled in clear glass light-exposed for a considerable amount of time leading to a loss of color and appearance of sulfur-like odors. The reaction involves riboflavin (RF), a highly photosensitive compound that undergoes to intermolecular photoreduction by the uptake of two electron equivalents from an external donor, the methionine. The reaction includes different steps forming methional which is extremely unstable and decomposes to methane thiol and acrolein. The reaction of two molecules of methane thiol yields dimethyl disulfide. Methane thiol is highly volatile, has a low perception threshold (2 to 10 µg/L in wine) and confers aroma-like rotten eggs or cabbage.