Macrowine 2021
IVES 9 IVES Conference Series 9 Multivariate strategies for red wines classification using stilbenes and flavonols content

Multivariate strategies for red wines classification using stilbenes and flavonols content

Abstract

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C). During such method validation, the selectivity was confirmed by a high resolution mass spectrometer (QTOF). For statistical analysis, four different data sets were used: wine varieties (34 samples), process influence (58 samples), harvest years (54 samples) and micro-regions (58 samples). The analysis of variance (ANOVA), principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were used. The Kennard-Stone algorithm was used to separate the samples into training and test sets. The leave-one-out cross validation method was used to choose the number of latent variables (LVs) in PLS-DA. The limits of detection (LOD) and quantification (LOQ) of (R), (Q), (M), (K), (V) and (C) were, respectively, 0.33 and 1.01, 0.30 and 0.90, 0.23 and 0.69, 0.27 and 0.81, 0.23 and 0.70, 0.02 and 0.05 µg mL-1. The concentration of (C) was below the LOQ in all samples, since it is consumed to synthesize the studied SaF. According to ANOVA, the SaF concentrations changed significantly due to the influence of the studied parameters. However, no patterns were observed in the scores of the first three principal components (PCs) of the PCA for harvest year and micro-regions data set. A tendency of separation was observed in the PCA scores for different varieties and processing data set. Through PLS-DA, it was possible to satisfactorily predict the wine variety and the processing through the concentration of SaF in terms of sensitivity and specificity. These figures of merit were between 67-100% for both data sets. These results indicate that the concentration of secondary metabolites trans-resveratrol, quercetin, myricetin, kaempferol and trans-e-viniferin, determined by HPLC-DAD, have the potential to measure the variation of red wines between micro-regions from “Campanha Gaúcha”. Consequently, it may be part of an efficient strategy to elaborate different styles of wines adapted to a whole region.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Letícia Silva*, Ana Bergold, Celito Crivellaro Guerra, Marcelo Marcelo, Marco Ferrão

*Embrapa and UFRGS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Proteomic and activity characterization of exocellular laccases from three Botrytis cinerea strains

Botrytis cinerea is a fungus that causes common infection in grapes and other fruits. In winemaking, its presence can be both considered desirable in the case of noble rot infection or undesirable when grey rot is developed. This fungus produces an extracellular enzyme known as laccase which is able to cause oxidation of phenolic compounds present in must and wine, causing most of the times a decrease in its quality and problems during the winemaking process [1]. Material and methods: Three B. cinerea strains (B0510, VA612 and RM344) were selected and grown in a liquid medium adapted from one previously described [2]. The enzyme was isolated by tangential ultrafiltration of the culture medium using a QuixStand system equipped with a 30 KDa filtration membrane.

South Africa’s top 10 Sauvignon blanc wines. How do the chemical and sensory profiles compare?

FNB Top 10 Sauvignon Blanc competition, presented by the Sauvignon Blanc Interest Group of South Africa and sponsored by First National Bank, is the country’s foremost platform for producers of this cultivar to showcase and benchmark their wines. Wines entered in the competition originated from all over the winegrowing regions of the country and the winning wines showed good representation of quality South African Sauvignon blanc wines. The ten selected wines were subjected to various chemical analyses including volatile thiol and methoxypyrazine determination, while the sensory profile of each wine was determined using projective mapping.

Anthropogenic factors in modulations of fungal populations from grapes to wines and their repercussions on wine characteristics

The effects of anthropogenic activities on vineyard (different plant protections) and in winery
(pressing/clarification step, addition of sulfur dioxide) on fungal populations from grape to wine were studied. The studied anthropogenic activities modify the fungal diversity. Thus, lower biodiversity of grapes from organic modality was measured for the three vintages considered compared to biodiversity from ecophyto modality and conventional modality. The pressing / clarification steps strongly modify fungal populations and the influence of the winery flora is highlighted.

Development of a new sustainable filtering media for wine and beer clarification and sterilisation

Different separation techniques are frequently used during vinification process. Nowadays, clarification and microbiological stabilization of wine or beer can be done using precoat filters or crossflow filters to remove yeast and bacteria. Kieselguhr powders are the most used filter aids for precoat filtration. Their crystalline structure and their pulverulent nature induce ecotoxicological risks when used. Moreover, regeneration and reuse of these filter aids is not efficient and the filtration waste requires cost effective retreatment.

On the losses of dissolved CO2 from laser-etched champagne glasses under standard tasting conditions

Under standard champagne tasting conditions, the complex interplay between the level of dissolved CO2 found in champagne, its temperature, the glass shape, and the bubbling rate, definitely impacts champagne tasting by modifying the neuro-physico-chemical mechanisms responsible for aroma release and flavor perception. Based on theoretical principles combining heterogeneous bubble nucleation, ascending bubble dynamics and mass transfer equations, a global model is proposed (depending on various parameters of both the wine and the glass itself), which quantitatively provides the progressive losses of dissolved CO2 from laser-etched champagne glasses.