Macrowine 2021
IVES 9 IVES Conference Series 9 Multivariate strategies for red wines classification using stilbenes and flavonols content

Multivariate strategies for red wines classification using stilbenes and flavonols content

Abstract

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C). During such method validation, the selectivity was confirmed by a high resolution mass spectrometer (QTOF). For statistical analysis, four different data sets were used: wine varieties (34 samples), process influence (58 samples), harvest years (54 samples) and micro-regions (58 samples). The analysis of variance (ANOVA), principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) were used. The Kennard-Stone algorithm was used to separate the samples into training and test sets. The leave-one-out cross validation method was used to choose the number of latent variables (LVs) in PLS-DA. The limits of detection (LOD) and quantification (LOQ) of (R), (Q), (M), (K), (V) and (C) were, respectively, 0.33 and 1.01, 0.30 and 0.90, 0.23 and 0.69, 0.27 and 0.81, 0.23 and 0.70, 0.02 and 0.05 µg mL-1. The concentration of (C) was below the LOQ in all samples, since it is consumed to synthesize the studied SaF. According to ANOVA, the SaF concentrations changed significantly due to the influence of the studied parameters. However, no patterns were observed in the scores of the first three principal components (PCs) of the PCA for harvest year and micro-regions data set. A tendency of separation was observed in the PCA scores for different varieties and processing data set. Through PLS-DA, it was possible to satisfactorily predict the wine variety and the processing through the concentration of SaF in terms of sensitivity and specificity. These figures of merit were between 67-100% for both data sets. These results indicate that the concentration of secondary metabolites trans-resveratrol, quercetin, myricetin, kaempferol and trans-e-viniferin, determined by HPLC-DAD, have the potential to measure the variation of red wines between micro-regions from “Campanha Gaúcha”. Consequently, it may be part of an efficient strategy to elaborate different styles of wines adapted to a whole region.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Letícia Silva*, Ana Bergold, Celito Crivellaro Guerra, Marcelo Marcelo, Marco Ferrão

*Embrapa and UFRGS

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Oligosaccharides in red wines: could their structure and composition be influenced by the grape-growing

Oligosaccharides have only recently been characterized in wine, and the information on composition and content is still limited. In wine, these molecules are mainly natural byproducts of the degradation of grape berry cell wall polysaccharides. Wine oligosaccharides present several physicochemical properties, being one relevant factor linked to the astringency perception of wines (1,2). A terroir can be defined as a grouping of homogeneous environmental units based on the typicality of the products obtained. This notion is particularly associated with wine, being the climate and the soil two of the major elements of terroir concept.

Elicitors used as a tool to increase stilbenes in grapes and wines

The economic importance of grapevine as a crop plant makes Vitis vinífera a good model system to study the improvement of the nutraceutical properties of food products (Vezulli et al. 2007). Stilbenes in general, and trans-resveratrol in particular, have been reported to be responsible for various beneficial effects. Resveratrol´s biological properties include antibacteria and antifungal effects, as well as cardioprotective, neuroprotective and anticâncer actions (Guerrero et al. 2010 ). Stilbenes can be induced by biotic and abiotic elicitors since they are phytoalexins (Bavaresco et al. 2001).

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.

Impact of drought stress on concentration and composition of wine proteins in Riesling

Protein haze in white wines is a major technological and economic problem of the wine industry. Field tests were carried out in steep slope vineyards planted with Riesling grapes over 3 dry growing seasons to study the effect of drought stress on the concentration of proteins in the resulting wines. Plots suffering from drought stress were compared with surrounding drip irrigated plots. Riesling grapes were processed into wines by conventional procedures. Protein amounts of the isolated wine colloids of the stressed samples were always higher than those of the watered samples(mean watered 13.8 ± 0.44, mean stressed 17.4 ± 0.40 g 100 g-1). As a consequence, higher bentonite doses were needed to achieve protein haze stability of the drought stressed treatments.

Microbial life in the grapevine: what can we expect from the leaf microbiome?

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents.