Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Effect of post-harvest ozone treatments on the skin phenolic composition and extractability of red winegrapes cv Nebbiolo and Barbera

Abstract

Wine industry is looking forward for innovative, safe and eco-friendly antimicrobial products allowing the reduction of chemical treatments in the grape defense and the winemaking process that can affect negatively the quality of the product. Ozone has been tested in food industry giving good results in preventing fungi and bacteria growth on a wide spectrum of vegetables and fruits, due to its oxidant activity and ability to attack numerous cellular constituents. Ozone leaves no chemical residues on the food surface, decomposing itself rapidly in oxygen. Gaseous ozone has been already tested for table grapes storage and on wine grapes during withering. In particular, ozone has been suggested as phenolic compounds elicitor, stimulating chemical defense mechanisms such as the synthesis of polyphenols, and as enhancer of cellular membrane and cell walls degradation phenomena. Phenolic compounds are strongly linked to the red wine quality, and their extraction depends on the grape variety, winemaking technique and cell wall degradation. In this work, Vitis vinifera L. cv Nebbiolo and Barbera, chosen for their different anthocyanin profiles, were post-harvest treated for 24 and 72 hours with gaseous ozone (30 µL/L). Untreated samples were used as control with the aim of investigating possible indirect physico-chemical effects of this sanitizing treatment on berry skin phenolic composition. Skin phenolic extractability was assessed during maceration (6, 24, 48, 96, 168 and 240 hours) using a wine-like solution, particularly for total anthocyanins (TA), proanthocyanidins (PRO) and flavanols reactive to vanillin (FRV), and anthocyanin profiles were also determined. Ozone did not affect significantly the final extraction yield of TA, PRO and FRV in Barbera; although anthocyanin extractability was higher in control rather than in ozone-treated samples during the first stages of maceration. Otherwise, Nebbiolo was positively influenced by the treatment because ozone increased significantly TA extraction (68.6, 64.2 and 59.9% for 24 hours ozone-treated berries, 72 hours ozone-treated berries and control samples, respectively). PRO and FRV extractability also showed an increase in both ozone-treated samples compared to the control (+8.6-9.1% for PRO and +7.3-11.7% for FRV). No significant differences were found among treatments for individual anthocyanins, whereas the variety and maceration time strongly affected the anthocyanin profile. In our experimental conditions, ozone enhanced phenolic compounds extractability in Nebbiolo grapes while it did not show significant effects on Barbera. Therefore, the use of ozone as sanitizing agent in the red varieties winemaking process can be considered because it did not negatively affect the extractability of skin anthocyanins and flavanols.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maria Alessandra Paissoni*, Cristian Carboni, Fabrizio Torchio, Francesco Cravero, Kalliopi Rantsiou, Luca Cocolin, Luca Rolle, Pierre-Louis Teissedre, Simone Giacosa, Susana Río Segade, Vasileios Englezos, Vincenzo Gerbi

*Università di Torino

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Identification, quantification and organoleptic impact of « dried fruit » molecular markers in Merlot and Cabernet Sauvignon grapes and in red wines

The aromas found in young Bordeaux red wines made with Merlot and Cabernet Sauvignon suggest a complex mixture of aromas of fresh red fruits such as cherry or blackberry for Merlot, and strawberry or blackcurrant for Cabernet Sauvignon. The aromas of these wines are closely linked with the maturity of the grapes. The climate change that has occurred during the last decade in Bordeaux has induced changes in the ripening conditions of grape berries. It is now widely admitted that over-ripening of the berries during hot and dry summers results in the development of characteristic flavors reminiscent of cooked fruits (fig, prune). The presence of these overriding odors found in both musts and young wines affects the quality and subtlety of the wine flavor and may shorten its shelf life.

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

The challenge of quality in sulphur dioxide free wines: natural polyphenol alternatives

Sulphur dioxide (SO2) seems indispensable in winemaking because of its properties. However, a current increasing concern about its allergies effects in food product has addressed the international research efforts on its replacement. This supposes a sufficient knowledge of its properties and conditions of use. Several studies compared SO2 properties against new alternatives that are supposed to overcome SO2 disadvantages. Firstly, the state of art on SO2 wine replacements is revised, and secondly, the last promising results using natural enriched polyphenol extracts are shown.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).