Macrowine 2021
IVES 9 IVES Conference Series 9 Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Abstract

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables. Samples were taken in a vineyard from 100% of veraison (24th February- 2014; 18.3±0.31°Brix) until technical maturity (14th April-2014; 24.4 ± 0.40°Brix). Total soluble (1) and insoluble polysaccharides (2), polysaccharides fractions by HPLC-RI (1) and anthocyanin and tannin extractability (3) were measured in five dates. Total soluble polysaccharides increase from 0.18 ± 0.03 at veraison to 0.50 ± 0.07g/g skin at technical maturity. In the same period insoluble polysaccharides decrease from 128.05 ± 9.33 to 69.00 ± 3.00 g/g skin. In the case of polysaccharides fractions (F) [neutral polysaccharides (F1), acid polysaccharides (F2) and oligosaccharides (F3)], only F2 change during the sampling dates increasing significantly its value in time. Anthocyanins and tannins increased their extractability in ≈ 18% and ≈ 10% in the sampling period, respectively. Pearson correlation coefficient between soluble polysaccharides and anthocyanin extractability was 0.86 and between soluble polysaccharides and tannin extractability was 0.76. The increase in soluble polysaccharides is closely related with anthocyanin and tannin extraction from grapes.

(1) Ayestarán, B., Z. Guadalupe, and D. León. 2004. Quantification of major grape polysaccharides (Tempranillo v.) released by macera¬tion enzymes during the fermentation process. Analytica Chim. Acta, 513(1): 29-39. (2) Hernandez-Hierro, J., Quijada-Morín, N., Martinez-Lapuente, L., Guadalupe, Z., Ayestarán, B., Rivas-Gonzalo, J. and M. Escribano-Bailón. 2014. Relationship between skin cell wall composition and anthocyanin extractability of Vitis vinifera L. cv. Tempranillo at different grape ripeness degree. Food Chem. 146(1): 41-47. (3) Saint-Cricqde Gaulejac N., Vivas N., Glories Y., 1998. Maturité phénolique: définition et contrôle.Rev. Franc. Oenol., 173, 22-25 Acknowledgements: This study was supported by FONDECYT N°1140882 and N°3150322 Projects.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Alvaro Peña-Neira*, Alvaro Peña-Neira, Claudio Pastenes, Elías Obreque Slier, Francisco Pavez-Roco, Mariona Gil Cortiella, Remigio López

*Universidad de Chile

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Condensed tannin is a principle group of polyphenol compounds derived from grape, greatly contributing to the bioactivity and the sensory perception of wine. Condensed tannins present as a heterogeneous mixture in nature involving various degrees of both polymerization and galloylation. Even though multiple attempts focusing on fractionation of grape condensed tannins by solid-phase have been conducted over the past decades, few individual tannins have been purified and identified. Hence, our knowledge on grape and wine condensed tannin moleculars has to be limited at the several known monomeric, dimeric and trimeric proanthocyanidins

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Effects of post-fermentative cold maceration on chemical and sensory characteristics of Syrah, Cabernet Franc and Montepulciano wines

Astringency sensation decreases slowly during the aging of red wine. Complex reactions of condensation and precipitation of wine polyphenols are involved in this phenomenon. Wine composition and conditions of aging, such as temperature and oxygen availability, strongly influence evolution of the phenol matrix. Recently, a Post-Fermentative cold Maceration (PFM) technique was tested with the aim of accelerating reactions leading to the reduction of astringency and exploiting chemical compounds not extracted from the solid parts of grapes during the previous traditional maceration phase. To this purpose, an innovative maceration system was engineered and used to perform PFM trials on marc derived from vinification of different varieties of red grapes.