Macrowine 2021
IVES 9 IVES Conference Series 9 Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Abstract

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables. Samples were taken in a vineyard from 100% of veraison (24th February- 2014; 18.3±0.31°Brix) until technical maturity (14th April-2014; 24.4 ± 0.40°Brix). Total soluble (1) and insoluble polysaccharides (2), polysaccharides fractions by HPLC-RI (1) and anthocyanin and tannin extractability (3) were measured in five dates. Total soluble polysaccharides increase from 0.18 ± 0.03 at veraison to 0.50 ± 0.07g/g skin at technical maturity. In the same period insoluble polysaccharides decrease from 128.05 ± 9.33 to 69.00 ± 3.00 g/g skin. In the case of polysaccharides fractions (F) [neutral polysaccharides (F1), acid polysaccharides (F2) and oligosaccharides (F3)], only F2 change during the sampling dates increasing significantly its value in time. Anthocyanins and tannins increased their extractability in ≈ 18% and ≈ 10% in the sampling period, respectively. Pearson correlation coefficient between soluble polysaccharides and anthocyanin extractability was 0.86 and between soluble polysaccharides and tannin extractability was 0.76. The increase in soluble polysaccharides is closely related with anthocyanin and tannin extraction from grapes.

(1) Ayestarán, B., Z. Guadalupe, and D. León. 2004. Quantification of major grape polysaccharides (Tempranillo v.) released by macera¬tion enzymes during the fermentation process. Analytica Chim. Acta, 513(1): 29-39. (2) Hernandez-Hierro, J., Quijada-Morín, N., Martinez-Lapuente, L., Guadalupe, Z., Ayestarán, B., Rivas-Gonzalo, J. and M. Escribano-Bailón. 2014. Relationship between skin cell wall composition and anthocyanin extractability of Vitis vinifera L. cv. Tempranillo at different grape ripeness degree. Food Chem. 146(1): 41-47. (3) Saint-Cricqde Gaulejac N., Vivas N., Glories Y., 1998. Maturité phénolique: définition et contrôle.Rev. Franc. Oenol., 173, 22-25 Acknowledgements: This study was supported by FONDECYT N°1140882 and N°3150322 Projects.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Alvaro Peña-Neira*, Alvaro Peña-Neira, Claudio Pastenes, Elías Obreque Slier, Francisco Pavez-Roco, Mariona Gil Cortiella, Remigio López

*Universidad de Chile

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effects of bottle closure type on sensory characteristics of Chasselas wines

Several winemaking operations, such as filtration, pumping, and racking, are known to potentially facilitate the incorporation of atmospheric O2 into the wine. Control of grape must oxidation is one key aspect in the management of white wine aroma expression, color stability and shelf-life extension. On the one hand, controlled must oxidation may help to remove highly reactive phenolic compounds, which otherwise could contribute to premature oxidation. And on the other hand, in certain cases of extreme protection of the must from O2 (e.g. pressing under inert atmosphere), it can help to preserve varietal aromas and natural must antioxidants.

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

Flavanol glycosides in grapes and wines : the key missing molecular intermediates in condensed tannin biosynthesis ?

Polyphenols are present in a wide variety of plants and foods such as tea, cacao and grape1. An important sub-class of these compounds is the flavanols present in grapes and wines as monomers (e.g (+)-catechin or (-)-epicatechin), or polymers also called condensed tannins or proanthocyanidins. They have important antioxidant properties2 but their biosynthesis remains partly unknown. Some recent studies have focused on the role of glycosylated intermediates that are involved in the transport of the monomers and may serve as precursors in the polymerization mechanism3, 4. The global objective of this work is to identify flavanol glycosides in grapes or wines, describe their structure and determine their abundance during grape development and in wine.

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines.