Macrowine 2021
IVES 9 IVES Conference Series 9 Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Ripening of cv. Cabernet Sauvignon grapes: polysaccharides fractions evolution and phenolic extractability

Abstract

Polysaccharides and more specifically pectins, make up a significant portion of the cell wall material of the plant cells including the grapes. During the fruit ripening the associated softening is related to the breakdown of the cell wall polysaccharides. During this process, it is expected that polysaccharides that are soluble in red wine will be formed influencing its texture. Anthocyanins are responsible for the wine color and tannins for the astringency, body and bitterness of the wine. In the skins, these compounds are located in the cell vacuoles and the barrier that conditions their extractability is the skin cell wall that may determine the mechanical resistance, the texture and the ease of processing berries. The aim of this work was study the evolution of the polysaccharides and the anthocyanin and tannin extractability during the ripening period in Cabernet Sauvignon grapes, trying to correlate these variables. Samples were taken in a vineyard from 100% of veraison (24th February- 2014; 18.3±0.31°Brix) until technical maturity (14th April-2014; 24.4 ± 0.40°Brix). Total soluble (1) and insoluble polysaccharides (2), polysaccharides fractions by HPLC-RI (1) and anthocyanin and tannin extractability (3) were measured in five dates. Total soluble polysaccharides increase from 0.18 ± 0.03 at veraison to 0.50 ± 0.07g/g skin at technical maturity. In the same period insoluble polysaccharides decrease from 128.05 ± 9.33 to 69.00 ± 3.00 g/g skin. In the case of polysaccharides fractions (F) [neutral polysaccharides (F1), acid polysaccharides (F2) and oligosaccharides (F3)], only F2 change during the sampling dates increasing significantly its value in time. Anthocyanins and tannins increased their extractability in ≈ 18% and ≈ 10% in the sampling period, respectively. Pearson correlation coefficient between soluble polysaccharides and anthocyanin extractability was 0.86 and between soluble polysaccharides and tannin extractability was 0.76. The increase in soluble polysaccharides is closely related with anthocyanin and tannin extraction from grapes.

(1) Ayestarán, B., Z. Guadalupe, and D. León. 2004. Quantification of major grape polysaccharides (Tempranillo v.) released by macera¬tion enzymes during the fermentation process. Analytica Chim. Acta, 513(1): 29-39. (2) Hernandez-Hierro, J., Quijada-Morín, N., Martinez-Lapuente, L., Guadalupe, Z., Ayestarán, B., Rivas-Gonzalo, J. and M. Escribano-Bailón. 2014. Relationship between skin cell wall composition and anthocyanin extractability of Vitis vinifera L. cv. Tempranillo at different grape ripeness degree. Food Chem. 146(1): 41-47. (3) Saint-Cricqde Gaulejac N., Vivas N., Glories Y., 1998. Maturité phénolique: définition et contrôle.Rev. Franc. Oenol., 173, 22-25 Acknowledgements: This study was supported by FONDECYT N°1140882 and N°3150322 Projects.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Alvaro Peña-Neira*, Alvaro Peña-Neira, Claudio Pastenes, Elías Obreque Slier, Francisco Pavez-Roco, Mariona Gil Cortiella, Remigio López

*Universidad de Chile

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Directed Evolution of Oenococcus oeni: optimising yeast-bacteria interactions for improved malolactic fermentation

Malolactic fermentation (MLF) is a secondary step in the vinification process and it follows alcoholic fermentation (AF) which is predominantly carried out by Saccharomyces cerevisiae. These two processes result in the degradation of metabolites to produce secondary metabolites which also contribute to the final wine flavour and quality. AF results in the production of ethanol and carbon dioxide from sugars and MLF stems from the degradation of L-malic acid (a dicarboxylic acid) to L-lactic acid (a monocarboxylic acid). The latter process results in a smoother texture as the acidity of the wine is reduced by the process, it also adds to the flavour complexity of the wine.

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.

Effect of the winemaking technology on the phenolic compounds, foam parameters in sparklig wines

Contribution Sparkling wines elaborated following the traditional method undergo a second fermentation in closed bottles of base wines, followed by aging of wines with lees for at least 9 months. Most of the sparkling wines elaborated are white and rosé ones, although the production of red ones is highly increasing. One of the initial problems in red sparkling wine processing is to obtain suitable base wines that should have moderate alcohol content and astringency and adequate color intensity; which is difficult to obtain when grapes must be harvested at low phenolic and industrial maturity stage. The low phenolic maturity degree in the red grapes makes essential to choose an adequate winemaking methodology to obtain the base wines because the extracted polyphenols will vary according the winemaking technique: carbonic maceration or destemmed-crushed grapes.

Influence of preflowering basal leaf removal on aromatic composition of cv. Tempranillo wine from semiarid climate (Extremadura Western Spain)

Abstract In this work the effects of early leaf removal performed manually at preflowering phenological stage, on the volatile composition of Tempranillo (Vitis vinifera L.) wines were studied. From 2009-2011 vintages 34 wine volatile compounds were identified and quantified by gas chromatography-mass spectrometry (GC-MS) where early leaf removal only modified 25 of them. The total C6 compounds, acetates and volatiles acids (with exception of isobutyric acid) were affected by defoliation, whereas alcohols and esters showed a minor effect. Furthermore the vintage effect also was shown.

Maturation of Agiorgitiko (Vitis vinifera) red wine on its wine lees: Impact on its phenolic composition

Maturation of wine on lees (often referred as sur lie) is a common practice applied by many winemakers around the world. In the past this method was applied mainly on white and/or sparkling wine production but recently also to red wine production. In our experiment, we matured red wine on wine lees of two origins: a) Light wine lees, collected after the completion of the alcoholic fermentation, b) Heavy lees, collected after the completion of the malolactic fermentation. The lees were free of off-odors and were added in the red wine in percentage 3% and 8%, simulating common winemaking addition. The maturation lasted in total six months and samples were collected for analysis after one, three and six months. During storage the lees were stirred.