Macrowine 2021
IVES 9 IVES Conference Series 9 Use of glutathione under different grape processing and winemaking conditions and its impact on the formation of sulfide off-flavors, colour, and sensory characteristics of Riesling, Sauvignon blanc, and Chardonnay

Use of glutathione under different grape processing and winemaking conditions and its impact on the formation of sulfide off-flavors, colour, and sensory characteristics of Riesling, Sauvignon blanc, and Chardonnay

Abstract

The use of glutathione (GSH) in winemaking has been legitimated recently, according to OIV resolutions OENO 445-2015 and OENO 446-2015 a maximum dose of 20 mg/L is now allowed to use in must and wine. Several studies have proven the benefits of GSH, predominantly in Sauvignon blanc. Thus, oxidative coloration of must and wine is limited, aroma compounds such as volatile thiols are preserved, and the development of ageing flavors such as sotolon and 2-aminoacetophenone is impeded. The protective effect may be explained by the high affinity of GSH to bind o-quinones which are formed during phenolic oxidation and which are known to initiate browning and other oxidative changes. Some researchers have proposed the hydroxycinnamic acid to GSH ratio (HGR) as an indicator of oxidation susceptibility of must and could show that lower ratios yielded lighter musts. In contrast to the advantages of GSH, other researchers found that GSH can foster the formation of H2S and other sulfide off-flavors during fermentation. Even during bottle aging, reductive odors may occur as a late consequence of high GSH levels during winemaking. In order to examine the impact of GSH on the formation of sulfide off-flavors, colour, and sensory characteristics, Riesling, Sauvignon blanc, and Chardonnay grapes were processed under different conditions to obtain musts with high and low phenolic content. Based on the original GSH concentration the HGR was adjusted using GSH or GSH-enriched IDY. The resulting wines were either racked off the lees or submitted to sur lie aging for 4 months. As already observed by others, GSH additions increased the GRP concentration in must and preserved their green color. At the same time, these musts tended to form higher concentrations of H2S, methyl and ethyl mercaptan during fermentation suggesting that excessive GSH is responsible for the production of volatile mercaptan metabolites. Normally, these compounds were degraded at the end of fermentation and dropped below sensory threshold as soon as the wines were racked off the gross lees. However, the decrease in mercaptan content, partly explained by the oxidative formation of disulfides, was strongly impaired when o-diphenols were low in concentration (e.g. in free run juice) or when musts were treated with ascorbic acid and SO2. This observation suggests that an effective mercaptan deodorization in young wines depends on the oxidizability of o-diphenols. Bottled wines were generally lower in GSH than musts. However, elevated levels of GSH could be determined after sur lie aging, possibly explaining the protection against oxidation in this aging regime. Sensory analysis after bottling revealed that the fruity odor of Riesling and Sauvignon blanc wines was enhanced when GSH was added to must in moderate concentrations. Excessive GSH, especially in musts with a low phenolic content (e.g. from whole-cluster pressing), could lead to sensorially noticeable sulfide off-flavor in the later wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Dominik Durner*, Hans-Georg Schmarr, Pascal Wegmann-Herr, Sebastian Ullrich, Ulrich Fischer

*DLR Rheinpfalz

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Innovations in the use of bentonite in enology: interactions with grape and wine proteins, colloids, polyphenols and aroma compounds.

The use of bentonite in oenology rounds around the limpidity and the stability that determine consumer acceptability. As a matter of fact, the haze formation in wine reduces its commercial value and makes it unacceptable for sale. Stabilization treatments are, therefore, essential to ensure a long-time limpidity and to forecast the formation of deposits in the bottle. Bentonite that is normally used in oenology for clarifying-fining purpose, shows a natural clay-based mineral structure allowing it to swell and to jelly in water and hence in must and wine.

Grape metabolites, aroma precursors and the complexities of wine flavour

A critical aspect of wine quality from a consumer perspective is the overall impression of wine flavour, which is formed by the interplay of volatile aroma compounds, their precursors, and taste and matrix components. Grapes contribute some potent aroma compounds, together with a large pool of non-volatile precursors (e.g. glycoconjugates and amino acid conjugates). Aroma precursors can break down through chemical hydrolysis reactions, or through the action of yeast or enzymes, significantly changing the aroma profile of a wine during winemaking and storage. In addition, glycoconjugates of monoterpenes, norisoprenoids and volatile phenols, together with sulfur-conjugates in wine, provide a reservoir of additional flavour through the in-mouth release of volatiles which may be perceived retro-nasally.

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.

Comparison of fortified, sfursat and passito winemaking techniques for the enhancement of the oenological potential of the black grape cultivar Moscato nero d’Acqui (Vitis vinifera L.)

One of the key factors of the economical development of viticulture and wine industry in specific limited areas is the exploitation of ancient, local grape varieties. Therefore, in recent years the growing interest to rediscover minor varieties, previously cultivated, has promoted many studies. With this regard, the focus of this study was the Vitis vinifera L. cultivar Moscato nero d’Acqui, nowadays found only in old vineyards in the Acqui zone (North-West Italy). In particular, the aims of this work were: i) to investigate secondary metabolites profile of the grapes, and ii) to evaluate the attitude to the production of special wines.

The impact of branched chain and aromatic amino acids on fermentation kinetics and aroma biosynthesis by wine yeast Saccharomyces cerevisiae

One of the major determinants of wine quality is the aroma. Wine aroma is the human perception of the matrix of grape and yeast derived volatiles and their interaction that contribute to flavour wine. Most common are higher alcohols, ester and aldehydes. In previous studies the formation of characteristic volatile compounds have been linked to the metabolism of branched-chain and aromatic amino acids
(BCAAs) in synthetic grape must. Here we report on an investigation to assess the impact of the initial amino acid concentration on the production of aroma compounds by the industrial yeast VIN13 grown in both synthetic and real grape musts.