Macrowine 2021
IVES 9 IVES Conference Series 9 Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Abstract

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation. In this study, the response of several grape genotypes to application of controlled doses of oxygen was investigated over three consecutive vintages. Healthy grapes were harvested at maturity form a single experimental vineyard and crushed in controlled conditions. The must obtained was submitted to three consecutive oxygen saturations (approx. 8 mg/L of oxygen). Oxygen consumption kinetics were measured using a chemioluminescence multisensor apparatus. Upon consumption of each saturation, samples were submitted to spectrophotometric analyses to assess oxidation-induced changes to relevant parameters such as absorbance at 280 nm, 320 nm and 420 nm. Voltammetric analyses were also carried out using a Nomasense Polyscan potentiostat with screen printed electrodes to assess the evolution of the entire must oxidizable fraction, including ortho-diphenols. Depending on the vintage, between four and eight genotypes were analyzed, with each oxidation experiment carried out in four replicates. Oxygen consumption rates varied considerably among samples and vintages. Genotypes could be divided in slow (0.07-0.17 mg/L/min) and fast (0.35-0.43 mg/L/min) oxygen consuming, and this was not clearly associate with Folin-Ciocalteu index. Minor changes were observed at the end of each oxidation cycle for UV-Vis parameters such as Abs 280 and 320, while Abs 420 generally increased, in particular during the 2015 trial. Electrochemical analysis revealed major changes in the content and profile of oxidizable compounds, which decreased with each oxidation cycle. The patterns of such changes, namely the regions of the voltammogram mostly affected by oxidation, were found to be genotype-dependent, with vintage only having a minor influence. Specific oxidation patterns could be associated with either slow or fast oxygen consuming musts. These data indicate that the response of grape must to oxidation is linked to specific compositional characteristics (phenolic profiles, enzymes etc) which can be more effectively investigated and controlled by electrochemical methods rather than conventional spectrophotometric approaches.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maurizio Ugliano*, Jean Baptiste Dieval, Nelly Champeau, Stephane Vidal, Stephanie Begrand

*University of Verona

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Efficiency of alternative chemical and physical treatments in reducing Brettanomyces Bruxellensis from oak wood

Oak barrels form an integral part of wine production, especially that of high quality wines. However, due to its porosity, wood presents an ecological niche for microbial proliferation and is highly susceptible to microbial spoilage which could cause considerable economic losses. Brettanomyces bruxellensis, the most commonly encountered microorganism responsible for spoilage during barrel ageing, can remain in barrels after barrel sanitation to contaminate new batches of wine after refilling. Therefore, effective sanitation treatments are of utmost importance to prevent recurring wine spoilage.

Study of the content of amino acids and biogenic amines in sparkling red wines

The production of red sparkling wines is lower in Spain in comparison with the winemaking of white or rosé sparkling wines. In red sparkling wine processing it is essential to obtain suitable base wines that should have moderate alcohol content, high acidity, good color values, an adequate mouth-feel and a sweet tannin. Grapes for sparkling wine production have to be harvested at low maturity stages, with lower alcohol contents and higher acidities, which will that the phenolic maturity of the grapes is also low, showing green tannins. This paper analyses different treatments in order to minimize these inconveniences: cold maceration-prefermentation and delestage to elaborate the grapes with lower maturity, must nanofiltration, and the partial osmosis of the wines made from grapes with an adequate maturity degree.

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Anthocyanin accumulation and extractability were studied in Tannat, Cabernet Sauvignon and Merlot grapes produced in the south of Uruguay in two consecutive seasons. Typical cultivation situations employed in the region for each variety were considered. A follow-up was carried out, considering 60 plants per vineyard, and the harvest was determined according to the technological indices of maturity. Samples of grapes were taken in duplicate in each vineyard periodically along grape maturation. The basic composition, polyphenolic potential and anthocyanin extractability were determined. Also, half of grapes were frozen and later peeled; skin extractions over 24 hs with a solution of 12% ethanol and pH 3.2 were carried out. The anthocyanin contents of the extracts obtained were determined by HPLC-DAD. The levels of anthocyanins reached the highest values before technological maturity. Anthocyanin extractability had a decrease during grape maturation.