Macrowine 2021
IVES 9 IVES Conference Series 9 Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Abstract

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation. In this study, the response of several grape genotypes to application of controlled doses of oxygen was investigated over three consecutive vintages. Healthy grapes were harvested at maturity form a single experimental vineyard and crushed in controlled conditions. The must obtained was submitted to three consecutive oxygen saturations (approx. 8 mg/L of oxygen). Oxygen consumption kinetics were measured using a chemioluminescence multisensor apparatus. Upon consumption of each saturation, samples were submitted to spectrophotometric analyses to assess oxidation-induced changes to relevant parameters such as absorbance at 280 nm, 320 nm and 420 nm. Voltammetric analyses were also carried out using a Nomasense Polyscan potentiostat with screen printed electrodes to assess the evolution of the entire must oxidizable fraction, including ortho-diphenols. Depending on the vintage, between four and eight genotypes were analyzed, with each oxidation experiment carried out in four replicates. Oxygen consumption rates varied considerably among samples and vintages. Genotypes could be divided in slow (0.07-0.17 mg/L/min) and fast (0.35-0.43 mg/L/min) oxygen consuming, and this was not clearly associate with Folin-Ciocalteu index. Minor changes were observed at the end of each oxidation cycle for UV-Vis parameters such as Abs 280 and 320, while Abs 420 generally increased, in particular during the 2015 trial. Electrochemical analysis revealed major changes in the content and profile of oxidizable compounds, which decreased with each oxidation cycle. The patterns of such changes, namely the regions of the voltammogram mostly affected by oxidation, were found to be genotype-dependent, with vintage only having a minor influence. Specific oxidation patterns could be associated with either slow or fast oxygen consuming musts. These data indicate that the response of grape must to oxidation is linked to specific compositional characteristics (phenolic profiles, enzymes etc) which can be more effectively investigated and controlled by electrochemical methods rather than conventional spectrophotometric approaches.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maurizio Ugliano*, Jean Baptiste Dieval, Nelly Champeau, Stephane Vidal, Stephanie Begrand

*University of Verona

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol).

Contribution of Piperitone to the mint nuances perceived in the aging bouquet of red Bordeaux wines

During the tasting of a fine, old wine, the aromas generated in the glass are intertwined in an intimate, complex manner, expressing the fragrance of the aging bouquet. This aging bouquet, which develops during bottle storage through a complex transformation process, may result in a broad palette of nuances. Among these, undergrowth, truffle, toasted, spicy, licorice, fresh red- and black-berry fruit and mint descriptors were recently identified as features of its olfactory representation for red Bordeaux wines. Although a targeted chemical approach focusing on volatile sulfur compounds revealed the role played by dimethyl sulfide, 2-furanmethanethiol, and 3-sulfanylhexanol as molecular markers of the typicality of the wine aging bouquet of red Bordeaux wines, its chemical transcription has only partially been elucidated.

Characterizing the effects of nitrogen on grapevines with different scion/rootstock combinations: agronomic, metabolomic and transcriptomic approaches

Most vineyards are grafted and include a variety (Vitis vinifera) grafted over a wild Vitis rootstock (hybrids of V. berlandieri, riparia and rupestris). Grape berry quality at harvest depends on a subtle balance between acidity and the concentrations of sugars, polyphenols and precursors of aroma compounds. The mechanisms controlling the balance of sugars/acids/polyphenols are influenced by the abiotic environment, in particular nitrogen supply, and interact with the genotypes of both the scion variety and the rootstock. Previous work suggests that some of the effects of water stress are in fact linked to a nitrogen deficiency driven indirectly by the reduction of water absorption.

Impact of heating must before fermentation on Chardonnay wines

Prefermentation steps of white winemaking are very important for controlling the stability and the sensory attributes of wines. Usually musts are clarified by cold settling to prevent the start of the fermentation, before racking big lees and thus limiting the appearance of vegetable or reduction off flavour while favouring an aromatic expression with low turbidity. Besides, to reach the protein stability, some white wines further require a bentonite fining, sometimes associated with negative effects on the sensory quality. This study aims to know the impact of musts heating after pressing on a Chardonnay wine in northern conditions by comparison with a classic cold racking of the must.

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.