Macrowine 2021
IVES 9 IVES Conference Series 9 Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Abstract

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation. In this study, the response of several grape genotypes to application of controlled doses of oxygen was investigated over three consecutive vintages. Healthy grapes were harvested at maturity form a single experimental vineyard and crushed in controlled conditions. The must obtained was submitted to three consecutive oxygen saturations (approx. 8 mg/L of oxygen). Oxygen consumption kinetics were measured using a chemioluminescence multisensor apparatus. Upon consumption of each saturation, samples were submitted to spectrophotometric analyses to assess oxidation-induced changes to relevant parameters such as absorbance at 280 nm, 320 nm and 420 nm. Voltammetric analyses were also carried out using a Nomasense Polyscan potentiostat with screen printed electrodes to assess the evolution of the entire must oxidizable fraction, including ortho-diphenols. Depending on the vintage, between four and eight genotypes were analyzed, with each oxidation experiment carried out in four replicates. Oxygen consumption rates varied considerably among samples and vintages. Genotypes could be divided in slow (0.07-0.17 mg/L/min) and fast (0.35-0.43 mg/L/min) oxygen consuming, and this was not clearly associate with Folin-Ciocalteu index. Minor changes were observed at the end of each oxidation cycle for UV-Vis parameters such as Abs 280 and 320, while Abs 420 generally increased, in particular during the 2015 trial. Electrochemical analysis revealed major changes in the content and profile of oxidizable compounds, which decreased with each oxidation cycle. The patterns of such changes, namely the regions of the voltammogram mostly affected by oxidation, were found to be genotype-dependent, with vintage only having a minor influence. Specific oxidation patterns could be associated with either slow or fast oxygen consuming musts. These data indicate that the response of grape must to oxidation is linked to specific compositional characteristics (phenolic profiles, enzymes etc) which can be more effectively investigated and controlled by electrochemical methods rather than conventional spectrophotometric approaches.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maurizio Ugliano*, Jean Baptiste Dieval, Nelly Champeau, Stephane Vidal, Stephanie Begrand

*University of Verona

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Intelligent article to control the internal pressure in continue in bottles

An intelligent packaging might, among others, provide information and allow monitoring of the quality of the packed product or its surrounding environment. A recent project on micro-flow wine bottles closed with aluminium screw cap and tightness liner, highlighted the importance of monitoring the internal overpressure continuously, in real-time and at least for 72 hours, since the internal pressure on the tightness liner and the micro-flow are related. Real-time and continuous measurements are not the standard methods of measurement of the overpressure, yet. The most used equipment for the determination of the pressure in wine bottle is the aphrometer, a destructive device that supplies a single value of pressure.

Analysis of off flavours in grapes infected with the fungal bunch rot pathogens, Aspergillus, Botrytis and Pencillium

Fungal bunch rots of grapes cause major losses to grape yield worldwide, yet the impact these moulds have on grape and wine quality is not well characterised. We sought to investigate the formation of unwanted volatile compounds of fungal origin in both synthetic grape juice culture media and in inoculated grape berries. Botrytis cinerea, Aspergillus niger, Aspergillus carbonarius, or Pencillium expansum were grown in synthetic grape juice medium and the culture homogenates analysed 4 and 7 days post inoculation. HS-SPME-GC-MS analysis of the culture homogenates 4 days post inoculation demonstrated that each of the fungi examined produced varying quantities of the mushroom or fungus-like aroma compounds, 1-Octen-3-ol, 1-Octen-3-one and 3-Octanone with A. carbonarius producing up to ten times the amounts of all three metabolites per mg of dry mycelium.

Update knowledge about the presence of condensed tannins in grapes and their contributions to astringency perception

Condensed tannin is a principle group of polyphenol compounds derived from grape, greatly contributing to the bioactivity and the sensory perception of wine. Condensed tannins present as a heterogeneous mixture in nature involving various degrees of both polymerization and galloylation. Even though multiple attempts focusing on fractionation of grape condensed tannins by solid-phase have been conducted over the past decades, few individual tannins have been purified and identified. Hence, our knowledge on grape and wine condensed tannin moleculars has to be limited at the several known monomeric, dimeric and trimeric proanthocyanidins

Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Anthocyanin accumulation and extractability were studied in Tannat, Cabernet Sauvignon and Merlot grapes produced in the south of Uruguay in two consecutive seasons. Typical cultivation situations employed in the region for each variety were considered. A follow-up was carried out, considering 60 plants per vineyard, and the harvest was determined according to the technological indices of maturity. Samples of grapes were taken in duplicate in each vineyard periodically along grape maturation. The basic composition, polyphenolic potential and anthocyanin extractability were determined. Also, half of grapes were frozen and later peeled; skin extractions over 24 hs with a solution of 12% ethanol and pH 3.2 were carried out. The anthocyanin contents of the extracts obtained were determined by HPLC-DAD. The levels of anthocyanins reached the highest values before technological maturity. Anthocyanin extractability had a decrease during grape maturation.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).