Macrowine 2021
IVES 9 IVES Conference Series 9 Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Abstract

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization. The lack of reliable tests assessing risk of protein clouding during bottle storage is a recurring problem of winemakers. The methods used to evaluate wine stability involve inducing haze formation (by heat, trichloroacetic acid, and ethanol) and then measuring of turbidity using spectrophotometers, turbidimeters or nephelometers. Currently used test assessing haze potential involves heating which often cause overdosing of fining agent. Moreover, it was shown that the composition of precipitate formed using above mentioned methods was not the same as naturally formed precipitate. A new and reliable method evaluating the haze potential, relevant to natural haze formation is needed. Different tests have been proposed to assess haze formation in wine. Most of these tests are based upon different types of procedures, leading to protein aggregation and precipitation. Heat stability trials, based on heat-induced precipitation, are the most common. These tests are empirical and do not necessarily reflect changes and destabilization phenomena liable to occur in real wine storage conditions. The fact that the same tests, associated with bentonite fining trials, are used to determine the bentonite doses needed to stabilize wines, leads to doses of bentonite much too high and consequently affecting wine quality. Given that the mechanisms underlying haze formation are still not fully understood, the aim of this work was to investigate the feasibility of using Dynamic light scattering (DLS) to understand the occurrence of haze formation, and the implication of wine compounds (protein, polyphenols, polysaccharides). DLS is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1 nm. DLS directly measures fluctuations in scattering intensity due to Brownian motion, which are analyzed to determine the translational diffusion coefficient Dt and hence an effective measure of molecular size, the hydrodynamic radius Rh. DLS could also provide a rough measure of size distributions in order to assess populations of aggregates, and characterize the colloidal stability of wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Benoit Bach*, Agnieszka Kosinska Cagnazzo, Julien Ducruet, Marc Mathieu, Wilfried Andlauer

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Technological possibilities of grape marc cell walls as wine fining agent. Effect on wine phenolic composition

Fining is a technique that is used to remove unwanted wine components that affect clarification, astringency, color, bitterness, and aroma. Fining involves the addition of adsorptive or reactive material in order to reduce or eliminate the presence of certain less desirable wine components and to ensure that a wine remains in a particular stable state for a given period of time Recently concerns have been raised about the addition of animal proteins, such as gelatin, to wine due to the disease known as bovine spongiform encephalopathy (Mad Cow disease). Although the origin of gelatins has been moved to porcine, winemakers are asking for substitute products with properties and application protocols similar to the traditional animal-derived ones, making the use of plant-derived proteins in fining a practically viable possibility. As a consequence, various fining agents derived from plants have been proposed, including proteins from cereals, legumes, and potato.

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation.

Molecular cloning and characterization of UDP-glucose: furaneol glucosyltransferase gene from Japanese

2,5-Dimethyl-4-hydroxy-3(2H)-furanone (furaneol) is an important aroma compound in fruits, such as pineapple and strawberry, and is reported to contribute to the strawberry-like note in some wines. Several grapevine species are used in winemaking, and furaneol is one of the characteristic aroma compounds in wines made from American grape (Vitis labrusca) and its hybrid grape, similar to methyl anthranilate. Muscat Bailey A is a hybrid grape variety [V. labrusca (Bailey) x V. vinifera (Muscat Hamburg)], and its wine is one of the most popular in Japan. The inclusion of Muscat Bailey A in the ‘International List of Vine and Varieties and their Synonyms’ managed by the ‘International Organisation of Vine and Wine (OIV)’ in 2013 has further fueled its popularity among winemakers and researchers worldwide.

Effect of nanofiltration on the chemical composition and wine quality

In Enology the conventional processes of filtration for clarification and stabilization are giving place to alternative membrane processes, including nanofiltration (NF). Furthermore, the increased alcohol content in wines recorded in recent years became an important issue for all the main wine producing countries. Among techniques available to the wine industry to reduce the ethanol content, NF is certainly one of the newest. This study is focused on the evaluation of NF influence on wine physical-chemical composition, including mineral content, which in accordance to our best knowledge is a novelty.

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.