Macrowine 2021
IVES 9 IVES Conference Series 9 Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Abstract

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization. The lack of reliable tests assessing risk of protein clouding during bottle storage is a recurring problem of winemakers. The methods used to evaluate wine stability involve inducing haze formation (by heat, trichloroacetic acid, and ethanol) and then measuring of turbidity using spectrophotometers, turbidimeters or nephelometers. Currently used test assessing haze potential involves heating which often cause overdosing of fining agent. Moreover, it was shown that the composition of precipitate formed using above mentioned methods was not the same as naturally formed precipitate. A new and reliable method evaluating the haze potential, relevant to natural haze formation is needed. Different tests have been proposed to assess haze formation in wine. Most of these tests are based upon different types of procedures, leading to protein aggregation and precipitation. Heat stability trials, based on heat-induced precipitation, are the most common. These tests are empirical and do not necessarily reflect changes and destabilization phenomena liable to occur in real wine storage conditions. The fact that the same tests, associated with bentonite fining trials, are used to determine the bentonite doses needed to stabilize wines, leads to doses of bentonite much too high and consequently affecting wine quality. Given that the mechanisms underlying haze formation are still not fully understood, the aim of this work was to investigate the feasibility of using Dynamic light scattering (DLS) to understand the occurrence of haze formation, and the implication of wine compounds (protein, polyphenols, polysaccharides). DLS is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1 nm. DLS directly measures fluctuations in scattering intensity due to Brownian motion, which are analyzed to determine the translational diffusion coefficient Dt and hence an effective measure of molecular size, the hydrodynamic radius Rh. DLS could also provide a rough measure of size distributions in order to assess populations of aggregates, and characterize the colloidal stability of wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Benoit Bach*, Agnieszka Kosinska Cagnazzo, Julien Ducruet, Marc Mathieu, Wilfried Andlauer

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effects of post-fermentative cold maceration on chemical and sensory characteristics of Syrah, Cabernet Franc and Montepulciano wines

Astringency sensation decreases slowly during the aging of red wine. Complex reactions of condensation and precipitation of wine polyphenols are involved in this phenomenon. Wine composition and conditions of aging, such as temperature and oxygen availability, strongly influence evolution of the phenol matrix. Recently, a Post-Fermentative cold Maceration (PFM) technique was tested with the aim of accelerating reactions leading to the reduction of astringency and exploiting chemical compounds not extracted from the solid parts of grapes during the previous traditional maceration phase. To this purpose, an innovative maceration system was engineered and used to perform PFM trials on marc derived from vinification of different varieties of red grapes.

The effect of cropload on the volatile aroma characteristics of ‘Beihong’ and ‘Beimei’ red wine

Beihong and Beimei were bred as winemaking cultivars released by Institute of Botany, the Chinese Academy of Sciences in 2008. The cultivars are selected from the population of ‘Muscat Hamburg’ (Vitis vinifera) ×V. amurensis. They are extended to most provinces in North of China because they have strong resistance to cold and disease and need not be buried in soil in winter. To better understand the effect of cropload on volatile compounds during wine-making, we surveyed volatiles composition and content of different cropload level in 3-years-old ‘Beihong’ and ‘Beimei’ vines which planted in east foot of Helan mountain of Ningxia (EHN).

Wood from barrique: release of phenolic compounds and permeability to oxygen

Chemical and sensory changes occurring in red wine during ageing in oak barrique are due to the slow and gradual entrance of oxygen along with a release of ellagic tannin from the wood. Though oxygen can enter the cask through the bunghole, it is not clear the role of permeation through the wood staves as well as the amount of oxygen entering by permeation. The distribution of the released ellagic tannins in the wine ageing is also unknown. The oxygen passing through the bunghole may have a different wine ageing effect compared to the oxygen permeating through the wooden staves owing to the uneven ellagic tannin concentration throughout the wine.

Comparison of fortified, sfursat and passito winemaking techniques for the enhancement of the oenological potential of the black grape cultivar Moscato nero d’Acqui (Vitis vinifera L.)

One of the key factors of the economical development of viticulture and wine industry in specific limited areas is the exploitation of ancient, local grape varieties. Therefore, in recent years the growing interest to rediscover minor varieties, previously cultivated, has promoted many studies. With this regard, the focus of this study was the Vitis vinifera L. cultivar Moscato nero d’Acqui, nowadays found only in old vineyards in the Acqui zone (North-West Italy). In particular, the aims of this work were: i) to investigate secondary metabolites profile of the grapes, and ii) to evaluate the attitude to the production of special wines.

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T