Macrowine 2021
IVES 9 IVES Conference Series 9 Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Abstract

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization. The lack of reliable tests assessing risk of protein clouding during bottle storage is a recurring problem of winemakers. The methods used to evaluate wine stability involve inducing haze formation (by heat, trichloroacetic acid, and ethanol) and then measuring of turbidity using spectrophotometers, turbidimeters or nephelometers. Currently used test assessing haze potential involves heating which often cause overdosing of fining agent. Moreover, it was shown that the composition of precipitate formed using above mentioned methods was not the same as naturally formed precipitate. A new and reliable method evaluating the haze potential, relevant to natural haze formation is needed. Different tests have been proposed to assess haze formation in wine. Most of these tests are based upon different types of procedures, leading to protein aggregation and precipitation. Heat stability trials, based on heat-induced precipitation, are the most common. These tests are empirical and do not necessarily reflect changes and destabilization phenomena liable to occur in real wine storage conditions. The fact that the same tests, associated with bentonite fining trials, are used to determine the bentonite doses needed to stabilize wines, leads to doses of bentonite much too high and consequently affecting wine quality. Given that the mechanisms underlying haze formation are still not fully understood, the aim of this work was to investigate the feasibility of using Dynamic light scattering (DLS) to understand the occurrence of haze formation, and the implication of wine compounds (protein, polyphenols, polysaccharides). DLS is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1 nm. DLS directly measures fluctuations in scattering intensity due to Brownian motion, which are analyzed to determine the translational diffusion coefficient Dt and hence an effective measure of molecular size, the hydrodynamic radius Rh. DLS could also provide a rough measure of size distributions in order to assess populations of aggregates, and characterize the colloidal stability of wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Benoit Bach*, Agnieszka Kosinska Cagnazzo, Julien Ducruet, Marc Mathieu, Wilfried Andlauer

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Anthocyanin accumulation and extractability during the maturation of the grapes of three varieties

Anthocyanin accumulation and extractability were studied in Tannat, Cabernet Sauvignon and Merlot grapes produced in the south of Uruguay in two consecutive seasons. Typical cultivation situations employed in the region for each variety were considered. A follow-up was carried out, considering 60 plants per vineyard, and the harvest was determined according to the technological indices of maturity. Samples of grapes were taken in duplicate in each vineyard periodically along grape maturation. The basic composition, polyphenolic potential and anthocyanin extractability were determined. Also, half of grapes were frozen and later peeled; skin extractions over 24 hs with a solution of 12% ethanol and pH 3.2 were carried out. The anthocyanin contents of the extracts obtained were determined by HPLC-DAD. The levels of anthocyanins reached the highest values before technological maturity. Anthocyanin extractability had a decrease during grape maturation.

Effect of nanofiltration on the chemical composition and wine quality

In Enology the conventional processes of filtration for clarification and stabilization are giving place to alternative membrane processes, including nanofiltration (NF). Furthermore, the increased alcohol content in wines recorded in recent years became an important issue for all the main wine producing countries. Among techniques available to the wine industry to reduce the ethanol content, NF is certainly one of the newest. This study is focused on the evaluation of NF influence on wine physical-chemical composition, including mineral content, which in accordance to our best knowledge is a novelty.

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.

Sensory and nephelometric analysis of tannin fractions obtained by ultrafiltration of red wines

The assessment of red wine mouthfeel relies primarily on the sensory description of its tannic properties. This evaluation could be improved by gaining a better understanding of the physicochemical properties of these tannins. Hence, the objectives of the present study were threefold: (1) to gain an insight into the sensory properties of subpopulations of proanthocyanidic tannins of different molecular sizes obtained through several ultrafiltration steps, (2) to quantify the kinetics of haze formation of these proanthocyanidic tannins in a dynamic polyvinylpyrrolidone (PVP) precipitation test, (3) to determine whether a correlation exists between the sensory and the precipitation data.

Multivariate strategies for red wines classification using stilbenes and flavonols content

Bioactive polyphenols from grapes and wines, like stilbenes and flavonols (SaF), are often determined to nutritional evaluation, but also for many other purposes. The objective of this study was to quantify SaF in red wines from “Campanha Gaúcha”, a large and young viticultural region from South Brazil. Moreover, through statistical analysis, evaluate the influence of these compounds according to varieties, production process, harvest years and micro-regions of cultivation. A total of 58 samples of red wines were analyzed by high-performance liquid chromatography coupled to diode array detector (HPLC-DAD) for determination of trans-resveratrol (R), quercetin (Q), myricetin (M), kaempferol (K), trans-e-viniferin (V) and their precursor, cinnamic acid (C).