Macrowine 2021
IVES 9 IVES Conference Series 9 Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Evaluation of colloidal stability in white and rosé wines investing Dynamic Light Scattering technology

Abstract

Proteins constitute one of the three main components of grape juice and white wine, phenolic compounds and polysaccharides being the others. A specific group of the total grape-derived proteins resists degradation or adsorption during the winemaking process and remains in finished wine if not removed by the commonplace commercial practice of bentonite fining. While bentonite is effective in removing the problematic proteins, it is claimed to adversely affect the quality of the treated wine under certain conditions, through the removal of colour, flavor and texture compounds. A number of studies have indicated that different protein fractions require distinct bentonite concentrations for protein removal and consequent heat stabilization. The lack of reliable tests assessing risk of protein clouding during bottle storage is a recurring problem of winemakers. The methods used to evaluate wine stability involve inducing haze formation (by heat, trichloroacetic acid, and ethanol) and then measuring of turbidity using spectrophotometers, turbidimeters or nephelometers. Currently used test assessing haze potential involves heating which often cause overdosing of fining agent. Moreover, it was shown that the composition of precipitate formed using above mentioned methods was not the same as naturally formed precipitate. A new and reliable method evaluating the haze potential, relevant to natural haze formation is needed. Different tests have been proposed to assess haze formation in wine. Most of these tests are based upon different types of procedures, leading to protein aggregation and precipitation. Heat stability trials, based on heat-induced precipitation, are the most common. These tests are empirical and do not necessarily reflect changes and destabilization phenomena liable to occur in real wine storage conditions. The fact that the same tests, associated with bentonite fining trials, are used to determine the bentonite doses needed to stabilize wines, leads to doses of bentonite much too high and consequently affecting wine quality. Given that the mechanisms underlying haze formation are still not fully understood, the aim of this work was to investigate the feasibility of using Dynamic light scattering (DLS) to understand the occurrence of haze formation, and the implication of wine compounds (protein, polyphenols, polysaccharides). DLS is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1 nm. DLS directly measures fluctuations in scattering intensity due to Brownian motion, which are analyzed to determine the translational diffusion coefficient Dt and hence an effective measure of molecular size, the hydrodynamic radius Rh. DLS could also provide a rough measure of size distributions in order to assess populations of aggregates, and characterize the colloidal stability of wines.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Benoit Bach*, Agnieszka Kosinska Cagnazzo, Julien Ducruet, Marc Mathieu, Wilfried Andlauer

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Metabolomics comparison of non-Saccharomyces yeasts in Sauvignon blanc and Shiraz

Saccharomyces cerevisiae (SC) is the main driver of alcoholic fermentation however, in wine, non-Saccharomyces species can have a powerful effect on aroma and flavor formation. This study aimed to compare untargeted volatile compound profiles from SPME-GC×GC-TOF-MS of Sauvignon blanc and Shiraz wine inoculated with six different non-Saccharomyces yeasts followed by SC. Torulaspora delbrueckii (TD), Lachancea thermotolerans (LT), Pichia kluyveri (PK) and Metschnikowia pulcherrima (MP) were commercial starter strains, while Candida zemplinina (CZ) and Kazachstania aerobia (KA), were isolated from wine grape environments. Each fermentation produced a distinct chemical profile that was unique for both grape musts. The SC-monoculture and CZ-SC sequential fermentations were the most distinctly different in the Sauvignon blanc while the LT-SC sequential fermentations were the most different from the control in the Shiraz fermentations.

What about oxygen transfer during wine aging in barrels?

During wine aging, several complex phenomena of gas transfer take place in barrels due to the wine/oak contact. The efficiency of this gas transfer varies according to oak wood’s intrinsic physical properties. This research aims to better understand oxygen transfer phenomena through dry oak staves and especially through stave gaps, in order to reevaluate the importance of barrel-making on a barrel’s supply of oxygen. Experimentation was based on the development of an innovative permeameter of laboratory scale, for which the principal operating conditions concerning applied pressure, the choice of liquid phase/gas phase, and the grain type of oak are taken into account and investigated. With a specially developed tightening system, the existing pressure at stave gaps in a barrel could be reproduced on a laboratory scale in order to estimate its influence on oxygen transfer efficiency.

Microbial stabilization of wines using innovative coiled UV-C reactor process: impact on chemical and organoleptic proprieties

For several years, numerous studies aimed at limiting the use of SO2 in wines (thermal treatments, pulsed electric fields, microwaves …). Processes must be able to preserve the organoleptic qualities of wines with low energy consumption. In this context, ultraviolet radiations (UV-C), at 254 nm, are well known for their germicidal proprieties. In order to inactivate microorganisms in grape juice and wine without affecting the quality of the product, efficiency of UV-C treatment process should be optimized.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

Use of computational modelling for selecting adsorbents for improved fining of wine

The occurrence of faults and taints in wine, such as those caused by microbial spoilage or various taints, have resulted in significant financial losses to wine producers. The wine industry commits significant financial resources towards fining and taint removal processes each year. Fining involves the addition of one or more adsorptive substrates to juice or wine to bind certain components, thus reducing their concentration [1]. However, these processes are often not selective and can also remove desirable flavour and aroma compounds.