Macrowine 2021
IVES 9 IVES Conference Series 9 Microbial life in the grapevine: what can we expect from the leaf microbiome?

Microbial life in the grapevine: what can we expect from the leaf microbiome?

Abstract

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents. In particular, these VOCs have been shown to promote root growth and thereby nutrient acquisition and growth, but also to induce plant resistance against diseases [2-4]. Their effects on fungal and oomycete pathogens range from mycelium growth reduction to inhibition of sporulation, zoospore release and even death, although much of these reports are based on experiments performed in controlled laboratory conditions with model plants [5]. Preliminary experiments indicate that these VOCs could also confer protection against oomycete pathogens grown in planta [6]. This presentation will summarize the present state of knowledge in both fields of research, the phyllosphere microbiome and the bacterial emission of VOCs, and highlight the potential these new fields offer for sustainable viticulture.

1. Vorholt JA. 2012. Microbial life in the phyllosphere. Nat Rev Micro 10:828-840. 2. Ryu CM, Farag MA, Hu CH, Reddy MS, Kloepper JW, Pare PW. 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol 134:1017-1026. 3. Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW. 2003. Bacterial volatiles promote growth in Arabidopsis. P Natl Acad Sci USA 100:4927-4932. 4. Bailly A, Groenhagen U, Schulz S, Geisler M, Eberl L, Weisskopf L. 2014. The inter-kingdom volatile signal indole promotes root development by interfering with auxin signalling. Plant J 80:758-771. 5. Weisskopf L. 2014. The potential of bacterial volatiles for crop protection against phytophathogenic fungi. In Méndez-Vilas A (ed.), Microbial pathogens and strategies for combating them: science, technology and education. Formatex Research Center, online resource. 6. DeVrieze M, Pandey P, Bucheli TD, Varadarajan AR, Ahrens CH, Weisskopf L, Bailly A. 2015. Volatile organic compounds from native potato-associated Pseudomonas as potential anti-oomycete agents. Front Microbiol 6.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Laure Weisskopf*

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages

An alternative to improve grape quality is the application to the vineyard of elicitors. Although these compounds were first used to increase resistance of plants against pathogens, it has been found that they are also able to induce mechanisms involved in the synthesis of phenolic compounds and some amino acids. However, researches about the influence of elicitors on grape volatile composition are scarcely. Therefore, the aim of this work was to study the influence of methyl jasmonate (MeJ) foliar application on grape aroma composition over three consecutive vintages. MeJ was applied to Tempranillo grapevines at a concentration of 10 mM in 2013, 2014, and 2015 years. Control plants were sprayed with water.

Ageing of Sauvignon Blanc white wines with Specific Inactivated Dry Yeasts: Effect on physical and chemical characteristics

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.

Characterization of commercial enological tannins and its effect on human saliva diffusion

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals.