Macrowine 2021
IVES 9 IVES Conference Series 9 Microbial life in the grapevine: what can we expect from the leaf microbiome?

Microbial life in the grapevine: what can we expect from the leaf microbiome?

Abstract

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents. In particular, these VOCs have been shown to promote root growth and thereby nutrient acquisition and growth, but also to induce plant resistance against diseases [2-4]. Their effects on fungal and oomycete pathogens range from mycelium growth reduction to inhibition of sporulation, zoospore release and even death, although much of these reports are based on experiments performed in controlled laboratory conditions with model plants [5]. Preliminary experiments indicate that these VOCs could also confer protection against oomycete pathogens grown in planta [6]. This presentation will summarize the present state of knowledge in both fields of research, the phyllosphere microbiome and the bacterial emission of VOCs, and highlight the potential these new fields offer for sustainable viticulture.

1. Vorholt JA. 2012. Microbial life in the phyllosphere. Nat Rev Micro 10:828-840. 2. Ryu CM, Farag MA, Hu CH, Reddy MS, Kloepper JW, Pare PW. 2004. Bacterial volatiles induce systemic resistance in Arabidopsis. Plant Physiol 134:1017-1026. 3. Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Pare PW, Kloepper JW. 2003. Bacterial volatiles promote growth in Arabidopsis. P Natl Acad Sci USA 100:4927-4932. 4. Bailly A, Groenhagen U, Schulz S, Geisler M, Eberl L, Weisskopf L. 2014. The inter-kingdom volatile signal indole promotes root development by interfering with auxin signalling. Plant J 80:758-771. 5. Weisskopf L. 2014. The potential of bacterial volatiles for crop protection against phytophathogenic fungi. In Méndez-Vilas A (ed.), Microbial pathogens and strategies for combating them: science, technology and education. Formatex Research Center, online resource. 6. DeVrieze M, Pandey P, Bucheli TD, Varadarajan AR, Ahrens CH, Weisskopf L, Bailly A. 2015. Volatile organic compounds from native potato-associated Pseudomonas as potential anti-oomycete agents. Front Microbiol 6.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Laure Weisskopf*

*HES-SO

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

The impact of different yeasts and harvest time on the wine quality of Beihong and Beimei (<I>V. vinifera x V. amurensis</I>)

Beihong and Beimei are two wine cultivars from ‘Muscat Hamberg’ (V. vinifera L.) and wild V. amurensis Rupr., which were released in China in 2008. Here,two enology practices were reported. Firstly, the impact of different yeasts including D254, GRE, K1, D21 and BDX on dry wine quality of Beihong and Beimei was investigated. For Beihong, among wines fermented by all yeasts, residual sugar content was the lowest, total anthocyanin and resveratrol contents were the highest in the wine by D254. However, the wine by D254 had lower titrable acid than those by the other yeasts except BDX.

WineMetrics: A new approach to unveil the “wine-like aroma” chemical feature

“The Human being has an excellent ability to detect and discriminate odors but typically has great difficulty in identifying specific odorants”(1). Furthermore, “from a cognitive point of view the mechanism used to judge wines is closer to pattern recognition than descriptive analysis.” Therefore, when one wants to reveal the volatile “wine-like feature” pattern recognition techniques are required. Sensomics is one of the most recent “omics”, i.e. a holistic perspective of a complex system, which deals with the description of substances originated from microorganism metabolism that are “active” to human senses (2). Depicting the relevant volatile fraction in wines has been an ongoing task in recent decades to which several research groups have allocated important resources. The most common strategy has been the “target approach” in order to identify the “key odorants” for a given wine varietal.

Analysis of peptide fraction from white wines

Among nitrogen compounds included in white wines, the peptide fraction is certainly the least studied, however this fraction is quantitatively the most important (Feuillat, 1974). Existing studies concern the fraction below 1 kDa and only for white and sparkling wines (Bartolomé et al, 1997, Desportes et al 2000). In this report, we have developed methods to isolate peptides from reference white wines. Then, we have applied this methodology with bitter wine to answer a research question: is there a relation between peptides and the bitterness of white wine as for some cheese for example (Furtado, 1984)?

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Contribution of Piperitone to the mint nuances perceived in the aging bouquet of red Bordeaux wines

During the tasting of a fine, old wine, the aromas generated in the glass are intertwined in an intimate, complex manner, expressing the fragrance of the aging bouquet. This aging bouquet, which develops during bottle storage through a complex transformation process, may result in a broad palette of nuances. Among these, undergrowth, truffle, toasted, spicy, licorice, fresh red- and black-berry fruit and mint descriptors were recently identified as features of its olfactory representation for red Bordeaux wines. Although a targeted chemical approach focusing on volatile sulfur compounds revealed the role played by dimethyl sulfide, 2-furanmethanethiol, and 3-sulfanylhexanol as molecular markers of the typicality of the wine aging bouquet of red Bordeaux wines, its chemical transcription has only partially been elucidated.