Macrowine 2021
IVES 9 IVES Conference Series 9 Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Abstract

Primary and secondary metabolites are major components of grape quality and wine typicity. Their accumulation is interconnected through a complex metabolic network, which is still not well understood. This study aims to investigate how the enzymes of central carbon metabolism interact with anthocyanin biosynthesis during grape berry development: does the accumulation of anthocyanins, which represents a non-negligible diversion of carbon metabolic fluxes, require reprogramming of central enzymes or is it controlled downstream of central metabolism? To this end, 23 enzymes involved in central carbon metabolism pathways have been analyzed in the berries of 3 grape cultivars, which have close genetic background but distinct temporal dynamics of anthocyanin accumulation. The 3 cultivars are 1) cv. Gamay, which has white flesh over berry development; 2) cv. Gamay de Bouze, which is a somatic mutant of cv. Gamay with white flesh at beginning of berry development and starts to accumulate anthocyanins in the flesh at the onset of fruit ripening; 3) cv. Gamay Fréaux, which is a somatic mutant of cv. Gamay de Bouze with flesh accumulating anthocyanins as early as fruit set. The temporal differences of anthocyanin accumulation of the three cultivars make them a valuable model system to study the interaction between primary and secondary metabolisms in grape berry. Berries of the three cultivars have been sampled at 11 times from fruit set to maturity. Primary metabolites (sugars, organic acids, and 21 free amino acids) and anthocyains have been analyzed, in conjunction with qPCR analysis of key genes involved in anthocyanin biosynthesis. The results showed that hexose concentrations are the same in the fleshes of the three cultivars; however, phenylalanine is much lower in the genotype that accumulates more anthocyanins. The expression of key genes involved in the anthocyanin biosynthesis pathway is in line with anthocyanin accumulation in each cultivar. Enzyme activity analysis also showed that enzymes involved in glycolysis (PGI, PGM) were highest in cv. Gamay Fréaux,and lowest in Gamay, in the same order as anthocyanin concentration. These results provide clues to modulate the balance between primary and secondary metabolites in grape berry. Acknowledgement: This work is partly supported by a grant from FR BIE “Biologie Intégrative et Ecologie” at Bordeaux University to ZD and YG.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Zhanwu Dai*, Christel Renaud, Eric Gomes, Ghislaine Hilbert, Jing Wu, Messa Meddar, Patricia Ballias, Serge Delrot, Yves Gibon

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Removal of Fumonisin B1 and B2 from red wine using polymeric substances

The Ability of PVPP (Polyvinylpolypyrrolidone), PVP-DEGMA-TAIC (copolimerization of N-vinyl-2-pyrrolidinone with ethylene glycol dimethacrylate and triallyl isocyanurate) and PAEGDMA
(poly(acrylamide-co-ethylene glycol dimethacrylate)) polymers was tested as removal agents for Fumonisin B1 (FB1) and Fumonisin B2 (FB2) from model solutions and red wine. The polymers removal capacity was checked at three different resident times (2, 8 and 24 hours of contact time between the polymer and the sample), showing no differences in the percentage of FB1 and FB2 removal. Then, different polymer concentrations (1, 5 and 10 mg mL-1) were tested in model solution with and without phenolics (i.e. gallic acid and 4-methylcatechol).

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Accumulation of polyphenols in Barbera and Nebbiolo leaves during the vegetative season

Grapevine berries produce thousands of secondary metabolites of diverse chemical nature that have been largely detailed in the past due to their importance for defining wine quality. The wide Vitis vinifera diversity, resulting in thousands of different varieties well detailed in many studies regarding berries, is still not investigated in vegetative organs, leaves in particular. Deepening knowledge related to this aspect could be of great interest for many reasons (for example the possibility of using leaf extract for pharmaceutical, cosmetic and nutrition purposes) but, above all, for understanding the susceptibility of different grapevine varieties to pathogens.

New acylated flavonols identified in the grape skin of Vitis vinifera cv. Tannat and their wines

Flavonols are a class of flavonoid compounds derived from plant secondary metabolism. There they play different roles like antioxidants, internal regulators and UV screenings. In red wines, flavonols have increasingly received consideration by part of scientific and winemakers according their properties began to arise known. Among these stand out wine colour stabilization and their value as bioactive compounds. In this work the complete series of the acetylated and p-coumaroylated derivatives of the 3-O-glycosides of methoxylated flavonols, namely isorhamnetin, laricitrin and syringetin, have been identified in grapes and their respective wines from Vitis vinifera cv. Tannat.

Oenological features of Sangiovese wine from vinification of whole grape berries

The present study was performed in a traditional winery located in the viticultural area of Brunello di Montalcino, Siena, Italy, in the vintage 2015. Actually, in this winery Sangiovese grape musts are fermented in large oak barrels by a single strain of Saccharomyces cerevisiae previously isolated in the same winery. Pumping over operations are carried out once or twice a day until the end of alcoholic fermentations. The aim of this work was to investigate on the oenological properties of Sangiovese wine produced with the traditional winemaking process adopted by the winery under study obtained from the fermentation of whole berries compared to that from crushed grape must. In particular, two lots of 65q of Sangiovese grapes from the same 3ha vineyard were vinified in 150hL oak barrels.