Macrowine 2021
IVES 9 IVES Conference Series 9 Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Abstract

Primary and secondary metabolites are major components of grape quality and wine typicity. Their accumulation is interconnected through a complex metabolic network, which is still not well understood. This study aims to investigate how the enzymes of central carbon metabolism interact with anthocyanin biosynthesis during grape berry development: does the accumulation of anthocyanins, which represents a non-negligible diversion of carbon metabolic fluxes, require reprogramming of central enzymes or is it controlled downstream of central metabolism? To this end, 23 enzymes involved in central carbon metabolism pathways have been analyzed in the berries of 3 grape cultivars, which have close genetic background but distinct temporal dynamics of anthocyanin accumulation. The 3 cultivars are 1) cv. Gamay, which has white flesh over berry development; 2) cv. Gamay de Bouze, which is a somatic mutant of cv. Gamay with white flesh at beginning of berry development and starts to accumulate anthocyanins in the flesh at the onset of fruit ripening; 3) cv. Gamay Fréaux, which is a somatic mutant of cv. Gamay de Bouze with flesh accumulating anthocyanins as early as fruit set. The temporal differences of anthocyanin accumulation of the three cultivars make them a valuable model system to study the interaction between primary and secondary metabolisms in grape berry. Berries of the three cultivars have been sampled at 11 times from fruit set to maturity. Primary metabolites (sugars, organic acids, and 21 free amino acids) and anthocyains have been analyzed, in conjunction with qPCR analysis of key genes involved in anthocyanin biosynthesis. The results showed that hexose concentrations are the same in the fleshes of the three cultivars; however, phenylalanine is much lower in the genotype that accumulates more anthocyanins. The expression of key genes involved in the anthocyanin biosynthesis pathway is in line with anthocyanin accumulation in each cultivar. Enzyme activity analysis also showed that enzymes involved in glycolysis (PGI, PGM) were highest in cv. Gamay Fréaux,and lowest in Gamay, in the same order as anthocyanin concentration. These results provide clues to modulate the balance between primary and secondary metabolites in grape berry. Acknowledgement: This work is partly supported by a grant from FR BIE “Biologie Intégrative et Ecologie” at Bordeaux University to ZD and YG.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Zhanwu Dai*, Christel Renaud, Eric Gomes, Ghislaine Hilbert, Jing Wu, Messa Meddar, Patricia Ballias, Serge Delrot, Yves Gibon

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

An excessive leaf-fruit ratio reduces the yeast assimilable nitrogen in the must

Yeast assimilable nitrogen (YAN) in the grape must is a key variable for wine quality as a source of aroma precursors. In a situation of YAN deficiency, a foliar urea application upon the vine at veraison enhances YAN concentration and facilitates must fermentation. In 2013, Agroscope investigated the impact of leaf-fruit ratio on the nitrogen (N) assimilation and partitioning in grapevine Vitis vinifera cv. Chasselas following foliar-urea application with the aim of improving its efficiency on the YAN concentration.

Impact of varying ethanol and carbonation levels on the odor threshold of 1,1,6-trimethyl-1,2-dihydronaphtalene (petrol off-flavor) and role of berry size and Riesling clones

1,1,6-trimethyl-1,2-dihydronaphtelene (TDN) evokes the odor of “petrol” in wine, especially in the variety Riesling. Increasing UV-radiation due to climate change intensifies formation of carotenoids in the berry skins and an increase of TDN-precursors1. Exploring new viticultural and oenological strategies to limit TDN formation in the future requires precise knowledge of TDN thresholds in different matrices. Thresholds reported in the literature vary substantially between 2 µg/L up to 20 µg/L2,3,4 due to the use of different methods. As Riesling grapes are used for very different wine styles such as dry, sweet or sparkling wines, it is essential to study the impact of varying ethanol and carbonation levels.

Study of the colour and phenolic evolution of three different tannin/anthocyanin ratios over time in a model wine

Phenolic compounds are important quality indicators in red wine. A large number of polyphenols play an important role in wine development, contributing to the colour and the sensory perception of the wines. Anthocyanins are the pigments responsible for the colour in young red wines while tannins are the principal contributors to the bitterness and the astringency of the wines. Wine polyphenols are considered more complex molecules than grape phenolics, due to the enormous number of chemical reactions which take place during the entire winemaking process and storage, forming more stable compounds.

Measurements of the oxygen dissolved in white wines elaborated in barrels without to open the bung of the barrels

Bases on oxoluminescence, we have developed an innovative device for measuring dissolved oxygen in wines in barrels without opening the bung. This system is directly inserted into the wood during the barrel elaboration and can be positioned at different locations of the barrel (the head, the hull …). During two successive vintages we have used this device notably to follow the oxygen dissolved of whites wines elaborated in barrels. This allowed us initially to monitor the oxygen levels of the harvest to bottling the whole elaboration process in barrels of white wines without using techniques of measurement suitable to modify the real values in wines (opening the bung to plunge an oximeter).

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.