Macrowine 2021
IVES 9 IVES Conference Series 9 Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Abstract

Primary and secondary metabolites are major components of grape quality and wine typicity. Their accumulation is interconnected through a complex metabolic network, which is still not well understood. This study aims to investigate how the enzymes of central carbon metabolism interact with anthocyanin biosynthesis during grape berry development: does the accumulation of anthocyanins, which represents a non-negligible diversion of carbon metabolic fluxes, require reprogramming of central enzymes or is it controlled downstream of central metabolism? To this end, 23 enzymes involved in central carbon metabolism pathways have been analyzed in the berries of 3 grape cultivars, which have close genetic background but distinct temporal dynamics of anthocyanin accumulation. The 3 cultivars are 1) cv. Gamay, which has white flesh over berry development; 2) cv. Gamay de Bouze, which is a somatic mutant of cv. Gamay with white flesh at beginning of berry development and starts to accumulate anthocyanins in the flesh at the onset of fruit ripening; 3) cv. Gamay Fréaux, which is a somatic mutant of cv. Gamay de Bouze with flesh accumulating anthocyanins as early as fruit set. The temporal differences of anthocyanin accumulation of the three cultivars make them a valuable model system to study the interaction between primary and secondary metabolisms in grape berry. Berries of the three cultivars have been sampled at 11 times from fruit set to maturity. Primary metabolites (sugars, organic acids, and 21 free amino acids) and anthocyains have been analyzed, in conjunction with qPCR analysis of key genes involved in anthocyanin biosynthesis. The results showed that hexose concentrations are the same in the fleshes of the three cultivars; however, phenylalanine is much lower in the genotype that accumulates more anthocyanins. The expression of key genes involved in the anthocyanin biosynthesis pathway is in line with anthocyanin accumulation in each cultivar. Enzyme activity analysis also showed that enzymes involved in glycolysis (PGI, PGM) were highest in cv. Gamay Fréaux,and lowest in Gamay, in the same order as anthocyanin concentration. These results provide clues to modulate the balance between primary and secondary metabolites in grape berry. Acknowledgement: This work is partly supported by a grant from FR BIE “Biologie Intégrative et Ecologie” at Bordeaux University to ZD and YG.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Zhanwu Dai*, Christel Renaud, Eric Gomes, Ghislaine Hilbert, Jing Wu, Messa Meddar, Patricia Ballias, Serge Delrot, Yves Gibon

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Anthropogenic factors in modulations of fungal populations from grapes to wines and their repercussions on wine characteristics

The effects of anthropogenic activities on vineyard (different plant protections) and in winery
(pressing/clarification step, addition of sulfur dioxide) on fungal populations from grape to wine were studied. The studied anthropogenic activities modify the fungal diversity. Thus, lower biodiversity of grapes from organic modality was measured for the three vintages considered compared to biodiversity from ecophyto modality and conventional modality. The pressing / clarification steps strongly modify fungal populations and the influence of the winery flora is highlighted.

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.

Impact of glutathione and elemental sulphur juice addition on the volatile thiol production in South African Sauvignon blanc wine

Three compounds, 3-mercaptohexanol (3MH), 3-mercaptohexyl-acetate (3MHA) and 4-mercapto-4-methylpentan-2-one (4MMP), also known as varietal thiols, have been identified to contribute positively to wine aroma and are responsible for the distinct gooseberry, grapefruit, guava and box tree character found in Sauvignon blanc wines. Certain volatile thiol compounds though, can cause off-aromas of onion, garlic, rubber and rotten egg, this group of molecules is known as reductive sulphur compounds (RSC). This study looks into how the addition of sulphur-compounds to Sauvignon blanc juice contributes to the varietal thiol (3MH and 3MHA) concentration and reductive sulphur compound concentration in South African Sauvignon blanc wine.

Ageing of Sauvignon Blanc white wines with Specific Inactivated Dry Yeasts: Effect on physical and chemical characteristics

Del Barrio-Galán, R.a, b, Gómez-Parrini, A.a, Peña-Neira, A.b a Lallemand Inc. Chile y Compañía Limitada, Rosario Norte 407, piso 6, Las condes, Santiago, Chile b Department of Agro-Industry and Enology, Faculty of Agronomical Sciences, University of Chile, Post Office Box 1004, Santa Rosa 11315, La Pintana, Santiago, Chile It is well known that polysaccharides, mainly mannoproteins, play an important role on physical, chemical and sensory quality of wines. The ageing of white wines on lees is used in order to release higher amounts of polysaccharides by the autolytic processes in order to obtain higher-quality wines. However, this technique is too slow, because the temperature and pH conditions are not the most suitable for this process. In addition, it can also involve certain disadvantages such as a greater demand on winery resources, a longer period of wine storage, the appearance of reduction notes and some microbiological alterations.

Grape byproducts as source of resveratrol oligomers for the development of antifungal extracts

Grape canes are a non-recycled byproduct of wine industry (1-5 tons per hectare per year) containing valuable phytochemicals of medicine and agronomical interest. Resveratrol and wine polyphenols are known to exert a plethora of health-promoting effects including antioxidant capacity, cardioprotection, anticancer activity, anti-inflammatory effects, and estrogenic/antiestrogenic properties (Guerrero et al. 2009). Additionally, resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance (Chang et al. 2011). Our project aims to develop polyphenol-rich grape cane extracts to fight phytopathogenic or clinically relevant fungi. We initiate the project with the development of analytical methods to analyze resveratrol mono- and oligomers (dimers, trimers and tetramers) from grape canes and we evaluate their potential activity against clinically relevant opportunistic fungal pathogens (Houillé et al. 2014).