Macrowine 2021
IVES 9 IVES Conference Series 9 Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Interaction between the enzymes of central carbon metabolism and anthocyanin biosynthesis during grape berry development

Abstract

Primary and secondary metabolites are major components of grape quality and wine typicity. Their accumulation is interconnected through a complex metabolic network, which is still not well understood. This study aims to investigate how the enzymes of central carbon metabolism interact with anthocyanin biosynthesis during grape berry development: does the accumulation of anthocyanins, which represents a non-negligible diversion of carbon metabolic fluxes, require reprogramming of central enzymes or is it controlled downstream of central metabolism? To this end, 23 enzymes involved in central carbon metabolism pathways have been analyzed in the berries of 3 grape cultivars, which have close genetic background but distinct temporal dynamics of anthocyanin accumulation. The 3 cultivars are 1) cv. Gamay, which has white flesh over berry development; 2) cv. Gamay de Bouze, which is a somatic mutant of cv. Gamay with white flesh at beginning of berry development and starts to accumulate anthocyanins in the flesh at the onset of fruit ripening; 3) cv. Gamay Fréaux, which is a somatic mutant of cv. Gamay de Bouze with flesh accumulating anthocyanins as early as fruit set. The temporal differences of anthocyanin accumulation of the three cultivars make them a valuable model system to study the interaction between primary and secondary metabolisms in grape berry. Berries of the three cultivars have been sampled at 11 times from fruit set to maturity. Primary metabolites (sugars, organic acids, and 21 free amino acids) and anthocyains have been analyzed, in conjunction with qPCR analysis of key genes involved in anthocyanin biosynthesis. The results showed that hexose concentrations are the same in the fleshes of the three cultivars; however, phenylalanine is much lower in the genotype that accumulates more anthocyanins. The expression of key genes involved in the anthocyanin biosynthesis pathway is in line with anthocyanin accumulation in each cultivar. Enzyme activity analysis also showed that enzymes involved in glycolysis (PGI, PGM) were highest in cv. Gamay Fréaux,and lowest in Gamay, in the same order as anthocyanin concentration. These results provide clues to modulate the balance between primary and secondary metabolites in grape berry. Acknowledgement: This work is partly supported by a grant from FR BIE “Biologie Intégrative et Ecologie” at Bordeaux University to ZD and YG.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Zhanwu Dai*, Christel Renaud, Eric Gomes, Ghislaine Hilbert, Jing Wu, Messa Meddar, Patricia Ballias, Serge Delrot, Yves Gibon

*INRA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Extraction of polyphenols from grape marc by supercritical fluid extraction (SFE) and evaluation of their ‘bioavailability’ as dietary supplements

In the winemaking process, several compounds that remain in the grape skins and seeds after the fermentation stage are bioactive-compounds (substances with potential beneficial effects on health) that can be extracted in order to recovery valuable substances with a high commercial value for the cosmetic, food (nutraceuticals) and pharmaceutical industries. The skins contain significant amounts of bioactive substances such as tannins (16-27%) and other polyphenolic compounds (2-6.5%) in particular, catechins, anthocyanins, proanthocyanins, quercetin , ellagic acid and resveratrol.

Novel contribution to the study of mouth-feel properties in wines

In general, there is a well-established lexicon related to wine aroma and taste properties; however mouth-feel-related vocabulary usually includes heterogeneous, multimodal and personalized terms. Gawel et al.
(2000) published a wheel related to mouthfeel properties of red wine. However, its use in scientific publications has been limited. The authors accepted that the approach had certain limitations as it included redundant and terms with hedonic tone and some others were absent. It is of high interest to generate a mouth-feel lexicon and finding the chemical compound or group of compounds responsible for such properties in red wine. In the present work a chemical fractionation method has been developed.

Microbial life in the grapevine: what can we expect from the leaf microbiome?

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents.

On the losses of dissolved CO2 during champagne aging

A misconception lingers in the minds of some wine consumers that Champagne wines don’t age. It’s largely a myth, certainly as far as the best cuvees are concerned. Actually, during the so-called autolysis period of time (in the closed bottle, after the “prise de mousse”), complex chemical reactions take place when the wine remains in contact with the dead yeast cells, which progressively bring complex and very much sought-after aromas to champagne. Nevertheless, despite their remarkable impermeability to liquid and air, caps or natural cork stoppers used to cork the bottles are not 100% hermetic with regard to gas transfers. Gas species therefore very slowly diffuse through the cap or cork stopper, along their respective inverse partial pressure. After the “prise de mousse”, because the partial pressure of CO2 in the bottleneck reaches up to 6 bars (at 12 °C), gaseous CO2 progressively diffuse from the bottle to the ambient air
(where the partial pressure of gaseous CO2 is only of order of 0,0004 bar).

Interest and impact of PVP/PVI (Polyvinylpyrrolidone/ Polyvinylimidazole) on winemaking and final quality of wines

Céline Sparrow a, Christophe Morge a, a SOFRALAB SAS, 79, av. A.A. Thévenet – CS 11031 – 51530 Magenta, France Consumers’ health and security force authorities to limit, in wine as in others food industry products, the concentration in « dangerous » molecules. Therefore the legal limit in heavy metals keeps on decreasing. As per proof EU regulation just decrease the stain concentration in wine from 0,2 to 0,15 mg/l. Certain changes , such as sodium arsenite treatment in vines, disappearance of brass in wineries to the benefit of stainless steel, limit even more the concentration of heavy metals in wines. But the use of copper derivates in vines treatments is difficult to replace. In the case of wine and its elaboration, the problem is even more complex. Indeed, regulation forces the wine producers to control the concentration of certain heavy metals in final wines.