terclim by ICS banner
IVES 9 IVES Conference Series 9 Juvenile-to-adult vegetative phase transition in grapevine 

Juvenile-to-adult vegetative phase transition in grapevine 

Abstract

The sequential activity of miR156 and miR172 controls the juvenile to adult phase transition in many plant species, where miR156 abundance decreases while miR172 increases along plant development. Very little is known about phase transition in horticultural woody species, which show substantially long vegetative phases. In grapevine, phase transition seems to be dissociated, displaying a first transition from juvenile to adult vegetative state in the first year, coincident with tendril differentiation and a subsequent induction of inflorescences in place of some of tendrils in later years under flowering inductive environmental conditions. Since grapevine is a highly heterozygous species, the generation of genetically homogeneous material for replicated transcriptomic analyses from seed-derived plants was a main challenge. Here, we present a detailed global gene expression analysis of the juvenile-to-adult phase transition during the development of grapevine plantlets grown from seeds. The RNA-seq analysis demonstrated that miR156 was significantly repressed in the grapevine’s adult phase, where the appearance of tendrils acts as a marker of the transition. Consistent with the results reported in other species, we observed the activation of several SPL genes, known to be targets of miR156, and providing evidence for the conservation of the regulatory module miR156-SPLs in grapevine. However, no variation was detected in the expression of miR172, a key determinant in the transition to flowering in other species. This could be explained considering that grapevines do not flower during the first years of growth. Interestingly, we were still able to observe the overexpression of several genes known to be involved in the floral meristem identity transition which were also been detected along tendril development, consistently with the proposed common ontogenetic origin of tendrils and inflorescences in the Vitaceae family.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Diego Lijavetzky1*, Yolanda Ferradás2,3, Carolina Royo3, José Miguel Martínez-Zapater3

1Instituto de Biología Agrícola de Mendoza (IBAM, CONICET-UNCuyo), Almirante Brown 500, M5528AHB. Chacras de Coria, Mendoza, Argentina
2Departamento de Biología Funcional, Universidade de Santiago de Compostela, Santiago de Compostela, Spain
3Instituto de Ciencias de la Vid y del Vino, Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, La Rioja, Spain

Contact the author*

Keywords

phase change, juvenile phase, flowering transition, tendril development, miRNA, RNA-seq.

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sélection génétique des variétés originelles d’Arménie, berceau de la viticulture mondiale

Armenia, a small country in the South of the Caucasus, has been rediscovering its wine-growing past since the discovery in 2007 of archaeological wine-growing remains dating back around 8,000 years. They are among the oldest in the world. Despite a great diversity of grape varieties, Armenian winegrowers did not have sufficiently organized genetic collections to produce plants and satisfy the growing demand for planting.

Varietal differences between Shiraz and Cabernet sauvignon wines revealed by yeast metabolism

This study investigated if compositional differences between Shiraz and Cabernet Sauvignon grape varieties could influence the production of yeast-derived compounds. This work was based on the analysis of 40 experimental red wines made in triplicate fermentations from grapes harvested from two consecutive vintages in New South Wales (Australia). Grapes were picked at three maturity stages using berry sugar accumulation as physiological indicator, from nine commercial vineyards located in three different climatic regions (temperate, temperate-warm and warm-hot). A range of 30 yeast-derived wine volatiles including esters and alcohols were quantified by HS/SPME-GC/MS. Ammonia, amino-acids and lipids were analysed in the corresponding grapes. The juice total soluble solids (°Brix) in addition to the wine alcohol and residual sugar levels were also measured. The influence of grape maturity on wine ester composition was also variety dependent, particularly for higher alcohol acetate and ethyl ester of branched acids. This study highlights that varietal differences observed in Shiraz and Cabernet Sauvignon wines involve fermentation-derived compounds irrespective of the site (soil, climate, viticultural practices).

Better understanding on the fungal chitosan and derivatives antiseptic effect on Brettanomyces bruxellensis in wine.

The addition of fungal chitosan in wine is allowed since 2009 to release some spoilage microorganisms such as Brettanomyces bruxellensis (OIV/OENO 338A/2009; EC 53/2011).

Characterization of the adaptive mechanisms of grapevine rootstocks to iron deficiency induced by lime stress

Iron (Fe) deficiency is one of the important nutritional disorders for grapevine growing in alkaline and calcareous soils. Although Fe is an abundant element in soil, several factors limiting its availability, particularly the high levels of calcium carbonate or bicarbonate in soil, leading to a remarkable reduction in grapevine growth and productivity. The use of Fe chlorosis-tolerant rootstocks seems to be a cost-effective and efficient way to maintain Fe balance. Morphological and physiological changes occur in plants to cope with low Fe availability, including enhancement of ferric chelate reductase activity and altering root system by increasing lateral roots and root hairs.

Natural sparkling wine pétillant naturel: technological features and sensory profile

The article presents the results of a study on the technological features of producing sparkling wines of the Pétillant Naturel (Pet-Nat) type, made using the ancestral method from the Muscat Ottonel and Pinot Noir grape varieties.