terclim by ICS banner
IVES 9 IVES Conference Series 9 Developmental and genetic mechanisms underlying seedlessness in grapevine somatic variants

Developmental and genetic mechanisms underlying seedlessness in grapevine somatic variants

Abstract

Seedless table grapes are greatly appreciated for fresh and dry consumption. There is also some interest in seedless winegrapes, because the combination of lower fruit set, smaller berries with higher skin/pulp ratio and looser bunches with the absence of seeds in crushed berries, a possible source of astringent tannins, might also have favorable effects on wine quality. The gene VviAGL11 has been shown to play a central role in stenospermocarpy in Sultanina, but the molecular bases of other sources of stenospermocarpy as well as of parthenocarpy have not been clarified yet. To help fill this gap, a genetic and phenotypic characterization of seedless somatic variants from other cultivars has been undertaken, with special emphasis on a parthenocarpic Sangiovese mutant known as Corinto Nero.
In vitro pollen germination tests, in vivo pollination trials, histological observation of female gametophyte development, and genetic analysis of seedlings have shown that Corinto Nero is incapable of forming seeds probably due to meiotic anomalies.
In addition to a pairwise transcriptomic comparison between the mutant and the seeded wild-type, we present here a comparative genomic analysis between Corinto Nero and 10 seeded clones of Sangiovese based on short-read resequencing to identify sequence and structural variation that may reveal candidate genes for parthenocarpy in Corinto Nero.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Laura Costantini1*§, Paula Moreno-Sanz, Anna Nebish3,4, Silvia Lorenzi1, Elvira d’Amato5, Mara Miculan6,8, Gabriele Magris6,7, Gabriele Di Gaspero6, Ivana Gribaudo9, Anna Schneider9, Maria Stella Grando2

1 Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Trento), Italy
2 Center Agriculture Food Environment (C3A), University of Trento, San Michele all’Adige (Trento), Italy
3 Departamento de Viticultura, Instituto de Ciencias de la Vid y del Vino (CSIC, UR, Gobierno de la Rioja), Logroño, Spain
4 Department of Genetics and Cytology, Yerevan State University, Yerevan, Armenia
5 Department of Physics, University of Trento, Povo (Trento), Italy
6 Istituto di Genomica Applicata, Udine, Italy
7 Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine (Udine), Italy
8 Center of Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Makkah, Saudi Arabia
9 Institute for Sustainable Plant Protection – Research Council of Italy, Grugliasco (Torino), Italy

§ Equally contributed

Contact the author*

Keywords

somatic variation, clones, seedlessness, reproductive development, genomic structural variation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The albarizas and the viticultural zoning of Jerez­-Xérès-Sherry and Manzanilla-Sanlúcar de Barrameda registered apellations of origin (Cadiz, Spain)

Le terme ”Albariza” (du latin “albus“, blanc) déterminait à l’origine un type particulier du terrain calcaire, mais à présent il sert aussi à définir les sols et la bibliographie géologique actuelle le cite également pour de roches sédimentaires originaires du Neogene Betic.

Modelling vine water stress during a critical period and potential yield reduction rate in European wine regions: a retrospective analysis

Most European vineyards are managed under rainfed conditions, where seasonal water deficit has become increasingly important. The flowering-veraison phenophase represents an important period for vine response to water stress, which is seldomly thoroughly evaluated. Therefore, we aim to quantify the flowering-veraison water stress levels using Crop Water Stress Indicator (CWSI) over 1986–2015 for important European wine regions, and to assess the respective potential Yield Lose Rate (YLR). Additionally, we also investigate whether an advanced flowering-veraison phase may help alleviating the water stress with improved yield. A process-based grapevine model STICS is employed, which has been extensively calibrated for flowering and veraison stages using observed data at 38 locations with 10 different grapevine varieties. Subsequently, the model is being implemented at the regional level, considering site-specific calibration results and gridded climate and soil datasets. The findings suggest wine regions with stronger flowering-veraison CWSI tend to have higher potential YLR. However, contrasting patterns are found between wine regions in France-Germany-Luxembourg and Italy-Portugal-Spain. The former tends to have slight-to-moderate drought conditions (CWSI<0.5) and a negligible-to-moderate YLR (<30%), whereas the latter possesses severe-to-extreme CWSI (>0.5) and substantial YLR (>40%). Wine regions prone to a high drought risk (CWSI>0.75) are also identified, which are concentrated in southern Mediterranean Europe. An advanced flowering-veraison phase may have benefited from cooler temperatures and a higher fraction of spring precipitation in wine regions of Italy-Portugal-Spain, resulting in alleviated CWSI and moderate reductions of YLR. For those of France-Germany-Luxembourg, this can have reduced flowering-veraison precipitation, but prevalent alleviations of YLR are also found, possibly because of shifted phase towards a cooler growing season with reduced evaporative demands. Overall, such a retrospective analysis might provide new insights towards better management of seasonal water deficit for conventionally vulnerable Mediterranean wine regions, but also for relatively cooler and wetter Central European regions.

Three Nebbiolo clone anthocyanin profile as affected by environmental conditions

Vitis vinifera ‘Nebbiolo’ cultivar is a 3’-subsituted anthocyanin prevalent wine variety. It is grown in North-West Italy for the production of high quality ageing wines. In the present work berry skin anthocyanin amounts and profiles of the clones CVT 308, CVT 423 and CVT 142 were studied in 2004 and in 2005 in four environmentally different locations of North-West Italy: Donnas (steep mountain area), Monforte (hilly area, with a pH of 8.1), Vezza (hilly area, with a pH of 8.2) and Lessona (plain area, with a pH of 4.8).

Développement de l’appareil végétatif et maturation du raisin sur quatre sols de Pomerol en 1995

The Pomerol vineyard, located 35 km east of Bordeaux, covers around 800 ha on the left bank of the Isle. There is a system of fluvial terraces with more or less coarse gravel and pebble spreading, resting on a Tertiary substratum ranging from the Middle to Upper Eocene to the Lower Oligocene (Dubreuilh, 1993). This interweaving of terraces of varying thickness results in a brutal superposition of differentiated materials which give rise to various types of soil. Several site studies in this sector of the Libounais show significant morphological and analytical differences from one point to another (Guilloux et al ., 1978; Duteau, 1982; Van Leeuwen et al.., 1989). The distribution of the soils of the Pomerol vineyard was studied and resulted in a cartography at 1/25000th (Merouge, 1995).

Variety “Rebula” (Vitis vinifera L.) determines the terroir Goriška brda “Collio” in Slovenia

A «terroir» is a group of vineyards from the same region, belonging to a specific appellation, and sharing the same type of soil, weather conditions, grapes and wine making savoir-faire, which contribute its specific personality to the wine. White wine variety «Rebula» or «Ribolla gialla» is a local and traditional variety, which is mentioned already in XIII. century like variety for tax paying and merchandise.