terclim by ICS banner
IVES 9 IVES Conference Series 9 Developmental and genetic mechanisms underlying seedlessness in grapevine somatic variants

Developmental and genetic mechanisms underlying seedlessness in grapevine somatic variants

Abstract

Seedless table grapes are greatly appreciated for fresh and dry consumption. There is also some interest in seedless winegrapes, because the combination of lower fruit set, smaller berries with higher skin/pulp ratio and looser bunches with the absence of seeds in crushed berries, a possible source of astringent tannins, might also have favorable effects on wine quality. The gene VviAGL11 has been shown to play a central role in stenospermocarpy in Sultanina, but the molecular bases of other sources of stenospermocarpy as well as of parthenocarpy have not been clarified yet. To help fill this gap, a genetic and phenotypic characterization of seedless somatic variants from other cultivars has been undertaken, with special emphasis on a parthenocarpic Sangiovese mutant known as Corinto Nero.
In vitro pollen germination tests, in vivo pollination trials, histological observation of female gametophyte development, and genetic analysis of seedlings have shown that Corinto Nero is incapable of forming seeds probably due to meiotic anomalies.
In addition to a pairwise transcriptomic comparison between the mutant and the seeded wild-type, we present here a comparative genomic analysis between Corinto Nero and 10 seeded clones of Sangiovese based on short-read resequencing to identify sequence and structural variation that may reveal candidate genes for parthenocarpy in Corinto Nero.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Laura Costantini1*§, Paula Moreno-Sanz, Anna Nebish3,4, Silvia Lorenzi1, Elvira d’Amato5, Mara Miculan6,8, Gabriele Magris6,7, Gabriele Di Gaspero6, Ivana Gribaudo9, Anna Schneider9, Maria Stella Grando2

1 Research and Innovation Centre, Fondazione Edmund Mach, San Michele all’Adige (Trento), Italy
2 Center Agriculture Food Environment (C3A), University of Trento, San Michele all’Adige (Trento), Italy
3 Departamento de Viticultura, Instituto de Ciencias de la Vid y del Vino (CSIC, UR, Gobierno de la Rioja), Logroño, Spain
4 Department of Genetics and Cytology, Yerevan State University, Yerevan, Armenia
5 Department of Physics, University of Trento, Povo (Trento), Italy
6 Istituto di Genomica Applicata, Udine, Italy
7 Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine (Udine), Italy
8 Center of Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Makkah, Saudi Arabia
9 Institute for Sustainable Plant Protection – Research Council of Italy, Grugliasco (Torino), Italy

§ Equally contributed

Contact the author*

Keywords

somatic variation, clones, seedlessness, reproductive development, genomic structural variation

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

First evidence of the variability of varietal thiol precursors in Gewürztraminer clones

Much has been already written regarding the gluthationylate (GSH-) and cisteinylate (Cys-) precursors of 3-mercaptohexan-1-ol (3MH) since the discovery of their presence in grapes and juices.

Adapting the vineyard to climate change in warm climate regions with cultural practices

Since the 1980s global regime shift, grape growers have been steadily adapting to a changing climate. These adaptations have preserved the region-climate-cultivar rapports that have established the global trade of wine with lucrative economic benefits since the middle of 17th century. The advent of using fractions of crop and actual evapotranspiration replacement in vineyards with the use of supplemental irrigation has furthered the adaptation of wine grape cultivation. The shift in trellis systems, as well as pruning methods from positioned shoot systems to sprawling canopies, as well as adapting the bearing surface from head-trained, cane-pruned to cordon-trained, spur-pruned systems have also aided in the adaptation of grapevine to warmer temperatures. In warm climates, the use of shade cloth or over-head shade films not only have aided in arresting the damage of heat waves, but also identified opportunities to reduce the evapotranspiration from vineyards, reducing environmental footprint of vineyard. Our increase in knowledge on how best to understand the response of grapevine to climate change was aided with the identification of solar radiation exposure biomarker that is now used for phenotyping cultivars in their adaptability to harsh environments. Using fruit-based metrics such as sugar-flavonoid relationships were shown to be better indicators of losses in berry integrity associated with a warming climate, rather than solely focusing on region-climate-cultivar rapports. The resilience of wine grape was further enhanced by exploitation of rootstock × scion combinations that can resist untoward droughts and warm temperatures by making more resilient grapevine combinations. Our understanding of soil-plant-atmosphere continuum in the vineyard has increased within the last 50 years in such a manner that growers are able to use no-till systems with the aid of arbuscular mycorrhiza fungi inoculation with permanent cover cropping making the vineyard more resilient to droughts and heat waves. In premium wine grape regions viticulture has successfully adapted to a rapidly changing climate thus far, but berry based metrics are raising a concern that we may be approaching a tipping point.

To a better understanding of the impact of vine nitrogen status on volatile thiols from plot to transcriptome level

Volatile thiols contribute largely to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless and non-volatile precursors found in the berry and the must. The effect of vine nitrogen status on 3SH and 4MSP in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) was investigated in this study.

Biomarker-based phenotyping of grapevine (vitis spp.) resistance to plasmopara viticola reveals interactions between pyramided resistance loci

Grape downy mildew, caused by plasmopara viticola, is one of the main diseases affecting viticulture worldwide and its control usually relies on frequent sprays with agrochemicals. Grapevine varieties resistant to p. Viticola represent an effective solution to control downy mildew and reduce the environmental impact of viticulture. Loci of resistance to p. Viticola (Rpv) have been introgressed from wild vitis species and some of them, like Rpv1, Rpv3.1 and Rpv10, are currently the most utilized genetic resources in grape breeding.

A fine scale study of temperature variability in the Saint-Emilion area (Bordeaux, France)

As the quality and typicity of wine are influenced by the climate, it is essential to have a good knowledge of climate variability, especially with regard to temperature, which has a great impact on vine behavior and grape ripening.