terclim by ICS banner
IVES 9 IVES Conference Series 9 Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Abstract

Grafting plants uses intrinsic healing processes to join two different plants together to create one functional organism. To further our understanding of the molecular changes occurring during graft union formation in grapevine, we characterized the metabolome and transcriptome of intact and wounded cuttings (with and without buds to represent scions and rootstocks respectively), and homo- and heterografts at 0 and 14 days after wounding/grafting. As over-wintering, dormant plant material was grafted, we also characterized the gene expression changes in the wood during bud burst and spring activation of growth. We observed an asymmetrical pattern of gene expression between above and below the graft interface, auxin and sugar related genes were up-regulated above the graft interface, while genes involved in stress responses were up-regulated below the graft interface. Many genes were differentially expressed between wounded cuttings and homografts, and between the different scion/rootstock combinations. By combining MapMan and gene ontology analysis, we identified several genes families potentially involved in grafting. Our results were consistent with previous work on other plant species, but we were able to identify some specificities linked to grafting in grapevine. By comparing the scion of homo- and hetero-grafts, we also show that grafting with a non-self-rootstock can influence scion gene expression 14 days after grafting. The combination of metabolomics and transcriptomics shows that the changes in gene expression were accompanied by corresponding changes in tissue metabolite concentrations.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Sarah Jane Cookson1*, Grégoire Loupit1#, Virginie Garcia1, Joseph Tran1, Céline Franc3, Gilles De Revel3, Josep Valls Fonayet2,3, Nathalie Ollat1

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882, Villenave d’Ornon, France
2 Bordeaux Metabolome Facility, MetaboHUB, PHENOMEEMPHASIS, 33140 Villenave dOrnon, France
3 Unité de recherche Œnologie, EA 4577, USC 1366 INRAE, ISVV, Université de Bordeaux, F33882 Villenave d’Ornon France

#current address:Laboratoire d’Ecologie des Hydrosystèmes Naturels et Anthropisés, CNRS, Université Lyon 1, France

Contact the author*

Keywords

scion, rootstock, grafting, callus, transcriptome, metabolite analysis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Impact of the ‘Pinot’-family on early ripening in cool climate viticulture varieties

‘Pinot Precoce Noir’ (PPN) is an early ripening clone of ‘Pinot Noir’ (PN). The phenological differentiation is visible by an about two weeks earlier onset of veraison. It was found that the early veraison locus Ver1 on chromosome 16, previously identified in ‘Calardis Musqué’, originated from PPN. A highly correlated SSR marker, namely GF16-Ver1, was developed and tested for its ability to molecularly differentiate between PPN and PN as well as its potential to trace individual descendants.

How sensor technologies combined with artificial intelligence increase the efficiency in grapevine breeding (research): current developments and future perspectives

Viticulture and grapevine breeding programs have to face and adapt to the rapidly changing growing conditions due to the ongoing climate change, the scarcity of resources and the demand for sustainability within the whole value chain of wine production. In times of highly effective and cost-efficient genotyping technologies routinely applied in plant research and breeding, the need for comparable high-speed and high-resolution phenotyping tools has increased substantially. The disciplines of grapevine research, breeding and precision viticulture picked up this demand – mostly independent from each other – by the development, validation and establishment of different sensor technologies in order to extend management strategies or to transform labor-intensive and expensive phenotyping.

Zoning of viticulture in Yugoslavia

The last official zoning of Viticulture in Yugoslavia was performed 1978. year, when (according to recommendation of OIV and European Economic Community), regions, sub regions and vineyards districts were established supposing that the varieties which will be exhibit ail the positive agro biological and technological characteristics.

Sensory and nephelometric analysis of tannin fractions obtained by ultrafiltration of red wines

The assessment of red wine mouthfeel relies primarily on the sensory description of its tannic properties. This evaluation could be improved by gaining a better understanding of the physicochemical properties of these tannins. Hence, the objectives of the present study were threefold: (1) to gain an insight into the sensory properties of subpopulations of proanthocyanidic tannins of different molecular sizes obtained through several ultrafiltration steps, (2) to quantify the kinetics of haze formation of these proanthocyanidic tannins in a dynamic polyvinylpyrrolidone (PVP) precipitation test, (3) to determine whether a correlation exists between the sensory and the precipitation data.

Loose clustered vignoles clones reduce late season fruit rots

‘Vignoles’ is an aromatic, white-fruited wine grape variety valued by growers and wineries in the Eastern United States. Vignoles is grown in diverse locations in New York, Missouri, Indiana, Ohio, Pennsylvania, Illinois, Nebraska and Michigan. Consumers recognize and value the variety for its special wine quality.