terclim by ICS banner
IVES 9 IVES Conference Series 9 Evaluation of wood starch content on bench grafting success rate in grapevine

Evaluation of wood starch content on bench grafting success rate in grapevine

Abstract

Since the emergence of phylloxera, grafting has been the most used propagation method in viticulture. Despite all the improvement measures implemented in the nurseries, it is frequent that graft success rates vary depending on the nursery process and scion/rootstock combinations. The reasons behind this unsatisfactory behaviour are still unknown and can be diverse, although carbohydrate reserves might be hypothesised to be crucial, since callus, root, and new tissue formation will be built based on them. In order to identify the effect of carbohydrates on grafting success, nine combinations were established based on the starch content in grapevine scionwoods (cv. Tempranillo clone VN69) and rootstocks cuttings (110 Richter clone 237) used for grafting: Low (L), Medium (M), High (H). To perform this work, more than 90 plants were omega grafted per carbohydrate content combination and, after the callusing period, transferred to the rooting field. In August, nine plants per combination were uprooted and the grafting success rate was recorded as well as the vegetative growth and root system characteristics (number and diameter). Likewise, histological and histochemical characterization (cellulose, starch, callose and lignin) was performed at the graft interface. The implications of carbohydrate content on success rate will be discussed, comparing the grafting success rates obtained with potential symptoms of incompatibility, irregular cell arrangement, slower vascular differentiation, or persistence of the necrotic layer.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Ana Villa-Llop1,2*, Ana Pina3,4, Patricia Irisarri3,4, Melany Jiménez1, Luis Gonzaga Santesteban1

1 Departement of Agronomy, Biotechnology and Food Science, Univ. Pública de Navarra, Pamplona, Navarra, Spain
2 Vitis Navarra Nursery, Larraga, Navarra, Spain
3 Departamento de Ciencia Vegetal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avenida Montañana 930, 50059, Zaragoza, Spain
4Instituto Agroalimentario de Aragón-IA2 (CITA-Universidad de Zaragoza), 50013 Zaragoza, Spain

Contact the author*

Keywords

histology, scion-rootstock interaction, starch, success rate, viticulture

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Tolerance to sunburn: a variable to consider in the context of climate change

Climate change effects on grapevine phenology and grape primary and secondary metabolites are well described in recent literature. Increasing frequency and intensity of heat waves may be responsible for important yield losses in the future. However, the impact of this event is not so well described in literature. The present study highlights the importance of grape variety tolerance as a mitigation tool to climate change.

Implications of the nature of organic mulches used in vineyards on grapevine water status, yield, berry quality and biological soil health  

Climate emergency is going to affect the agricultural suistainability, wine grapes being probably one of the crops more sensitive to environmental constraints. In this context, mitigation strategies such as the revalorization of agricultural wastes are paramount to cope with the current challenges. The use of organic mulches has been reported to reduce soil water evaporation and improve vine water status, reduce soil erosion, and increase soil organic matter with little impact on berry quality. However, less is known about their effects on the microbiote of vineyards.

Contribution of seeds to red wine phenolic composition

Tannin composition is an important attribute in red wine quality, and it is therefore critical to understand the factors influencing tannin extraction during alcoholic fermentation. Tannins contribute to the mouthfeel of wines, but they also form pigmented polymers...

The invasive seaweed Rugulopteryx okamurae: an innovative plant protective extract

Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease worldwide. Most commercially important cultivars of the European grapevine are highly susceptible and therefore require the recurrent application of synthetic fungicides to control the disease, copper being the most frequently used. However, with European Union goals to lower their usage, there is a need to develop innovative and sustainable strategies. In this respect, seaweeds have proven to have great potential as phytosanitary agents, in addition to promoting plant growth and stress-tolerance.

Spatial suitability analysis for site selection of vineyards using biophysical models and computational intelligence

Developing a sustainable agricultural production system and acquiring the full potential of land resources requires employing land-use assessment. This entails knowledge of the climate, soil, and topography of the area of interest.