terclim by ICS banner
IVES 9 IVES Conference Series 9 NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

Abstract

Considering that global warming is changing berry ripening timing and progression, uncovering the molecular mechanisms and identifying key regulators governing berry ripening could provide important tools in maintaining high quality grapes and wine. NAC (NAM/ATAF/CUC) transcription factors represent an interesting family due to their key role in the developmental processes control, such as fruit-ripening-associated genes expression, and in the regulation of multiple stress responses. Between the 74 NAC family members, we selected 12 of them as putative regulators of berry ripening: NAC01, NAC03, NAC05, NAC11, NAC13, NAC17, NAC18, NAC26, NAC33, NAC37, NAC60 and NAC61. Genome wide analyses and functional assays permitted to reconstruct a hierarchical intra-family regulatory network in which most of the selected NACs resulted as transcriptional activators of other NACs. Moreover, to investigate the common regulative role of the selected NACs on the grapevine transcriptome, all the annotated V. vinifera genes were listed and the most represented genes between all the DAP-seq results were identified. Interestingly, at the top of the ranking we found many genes related to maturation and senescence such as an indole-3-acetic acid-amido synthetase, which could be involved in the establishment and maintenance of low IAA concentrations in ripening berries, a laccase, encoding for a phenylpropanoid pathway-related enzyme, the senescence-inducible chloroplast stay-green protein 1, triggering Chl degradation, and the UTP-glucose-1-phosphate uridylyltransferase, encoding for a carbohydrate-metabolism-related enzyme which is highly expressed in berries at veraison. All these results lay a foundation stone in understanding the genetic regulation of such a complex process as fruit ripening.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chiara Foresti1*, Alessandra Amato1, Luis Orduña2, Chiara Fattorini1, Erica D’Incà1, Nicola Vitulo1, José Tomás Matus2, Sara Zenoni1

1Department of Biotechnology, University of Verona, Verona, Italy.
2Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Valencia, Spain

Contact the author*

Keywords

Berry ripening, cistrome, NAC, hierarchical intra-family network

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Volatile compounds as indicators of terroir differentiation in Moldovan Feteasca Neagra wines

This study examined volatile compounds in Feteasca Neagra wines from seven vineyards across three PGI regions in Moldova using GC-IMS.

Light-struck taste in white wine: enological approach for its prevention

Light-struck taste is a defect prevalent in white wines bottled in clear glass light-exposed for a considerable amount of time leading to a loss of color and appearance of sulfur-like odors. The reaction involves riboflavin (RF), a highly photosensitive compound that undergoes to intermolecular photoreduction by the uptake of two electron equivalents from an external donor, the methionine. The reaction includes different steps forming methional which is extremely unstable and decomposes to methane thiol and acrolein. The reaction of two molecules of methane thiol yields dimethyl disulfide. Methane thiol is highly volatile, has a low perception threshold (2 to 10 µg/L in wine) and confers aroma-like rotten eggs or cabbage.

Partial dealcoholisation of red wine by reverse osmosis-evaporative perstraction: impact on wine composition

Around the world, the alcohol content of wine has been steadily increasing; partly as a consequence of climate change, but also due to improvements in viticultural management practices and winemaking techniques [1,2]. Concurrently, market demand for wines with lower alcohol levels has increased as consumers seek to reduce alcohol intake for social and/or health reasons [3]. As such, there is increasing demand for both innovative methods that allow winemakers to produce ‘reduced alcohol wines’ (RAW) and a better understanding of the impact of such methods on the composition of RAW. This study therefore aimed to investigate compositional changes in two red wines resulting from partial alcohol removal following treatment by one such method, involving a combination of reverse osmosis and evaporative perstraction (RO-EP).

ACCUMULATION OF GRAPE METABOLITES IS DIFFERENTLY IMPACTED BY WATER DEFICIT AT THE BERRY AND PLANT LEVELS IN NEW FUNGUS DISEASE-TOLERANT GENOTYPES

The use of new fungus disease-tolerant varieties is a promising long-term solution to better manage chemical input in viticulture, but unfortunately little is known regarding these new hybrids fruit development and metabolites accumulation in front of abiotic stresses such as water deficit (WD). Thus, prior to the adoption of such varieties by the wine industry in Mediterranean regions, there is a need to consider their suitability to WD.

Un Système d’Informations à Références Spatiales sur le Vignoble. Un outil performant d’aide aux recherches sur la caractérisation des terroirs viticoles

The “Terroirs d’Anjou” project led by the Agronomy sector of the Vine and Wine Research Unit of the INRA center in Angers aims to characterize the viticultural terroirs in a study area which includes 29 municipalities in the Maine et Loire and cuts across the Anjou, Coteaux du layon and Coteaux de l’Aubance appellation areas.