terclim by ICS banner
IVES 9 IVES Conference Series 9 NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

Abstract

Considering that global warming is changing berry ripening timing and progression, uncovering the molecular mechanisms and identifying key regulators governing berry ripening could provide important tools in maintaining high quality grapes and wine. NAC (NAM/ATAF/CUC) transcription factors represent an interesting family due to their key role in the developmental processes control, such as fruit-ripening-associated genes expression, and in the regulation of multiple stress responses. Between the 74 NAC family members, we selected 12 of them as putative regulators of berry ripening: NAC01, NAC03, NAC05, NAC11, NAC13, NAC17, NAC18, NAC26, NAC33, NAC37, NAC60 and NAC61. Genome wide analyses and functional assays permitted to reconstruct a hierarchical intra-family regulatory network in which most of the selected NACs resulted as transcriptional activators of other NACs. Moreover, to investigate the common regulative role of the selected NACs on the grapevine transcriptome, all the annotated V. vinifera genes were listed and the most represented genes between all the DAP-seq results were identified. Interestingly, at the top of the ranking we found many genes related to maturation and senescence such as an indole-3-acetic acid-amido synthetase, which could be involved in the establishment and maintenance of low IAA concentrations in ripening berries, a laccase, encoding for a phenylpropanoid pathway-related enzyme, the senescence-inducible chloroplast stay-green protein 1, triggering Chl degradation, and the UTP-glucose-1-phosphate uridylyltransferase, encoding for a carbohydrate-metabolism-related enzyme which is highly expressed in berries at veraison. All these results lay a foundation stone in understanding the genetic regulation of such a complex process as fruit ripening.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chiara Foresti1*, Alessandra Amato1, Luis Orduña2, Chiara Fattorini1, Erica D’Incà1, Nicola Vitulo1, José Tomás Matus2, Sara Zenoni1

1Department of Biotechnology, University of Verona, Verona, Italy.
2Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Valencia, Spain

Contact the author*

Keywords

Berry ripening, cistrome, NAC, hierarchical intra-family network

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

On the losses of dissolved CO2 during champagne aging

A misconception lingers in the minds of some wine consumers that Champagne wines don’t age. It’s largely a myth, certainly as far as the best cuvees are concerned. Actually, during the so-called autolysis period of time (in the closed bottle, after the “prise de mousse”), complex chemical reactions take place when the wine remains in contact with the dead yeast cells, which progressively bring complex and very much sought-after aromas to champagne. Nevertheless, despite their remarkable impermeability to liquid and air, caps or natural cork stoppers used to cork the bottles are not 100% hermetic with regard to gas transfers. Gas species therefore very slowly diffuse through the cap or cork stopper, along their respective inverse partial pressure. After the “prise de mousse”, because the partial pressure of CO2 in the bottleneck reaches up to 6 bars (at 12 °C), gaseous CO2 progressively diffuse from the bottle to the ambient air
(where the partial pressure of gaseous CO2 is only of order of 0,0004 bar).

Estimation of chemical age of red wines with the use of Fourier transform infrared spectroscopy (FT-IR) and chemometrics

The color of a red wine is one of the most important parameters of its quality, giving much information on its status, such as the grape variety used or the winemaking style. As the result of a complex equilibrium between different forms of anthocyanins and polymerization reactions which occur over the course of time, color can also serve as an indication of a wines’ age. For this purpose the “chemical age” i and ii indexes have been introduced by Somers in 1977. The chemical age index i measures the color absorbance after the addition of acetaldehyde while chemical index ii provides an indication of how much of the total red pigments are resistant to SO2 bleaching.

The FEM grapevine crossbreeding program for resistance to the main ampelopathies: towards climate-resilient varieties

The technique of crossing, whether free or controlled, has always been a source of variability allowing the selection of new varieties with improved fitness.

Using RGB images and LiDAR data to characterise fruit-to-leaf ratios in grapevine collections

One of the main effects of global warming is an increase in the sugar concentration of grapes at harvest time, resulting in wines with a high alcohol content and an unbalanced structure. The fruit to leaf ratio is a key factor in determining the final sugar concentration, and training systems and management techniques can help to control this parameter.

Characterization of 25 white grape varieties from the variety collection of ICVV (D.O.Ca.Rioja, Spain)

The effects of climate change produce an increase in sugar concentration and a decrease in acidity, without reaching the optimum grape phenolic maturity [1]. The aim of this work was to characterize 25 white grape varieties