terclim by ICS banner
IVES 9 IVES Conference Series 9 NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

Abstract

Considering that global warming is changing berry ripening timing and progression, uncovering the molecular mechanisms and identifying key regulators governing berry ripening could provide important tools in maintaining high quality grapes and wine. NAC (NAM/ATAF/CUC) transcription factors represent an interesting family due to their key role in the developmental processes control, such as fruit-ripening-associated genes expression, and in the regulation of multiple stress responses. Between the 74 NAC family members, we selected 12 of them as putative regulators of berry ripening: NAC01, NAC03, NAC05, NAC11, NAC13, NAC17, NAC18, NAC26, NAC33, NAC37, NAC60 and NAC61. Genome wide analyses and functional assays permitted to reconstruct a hierarchical intra-family regulatory network in which most of the selected NACs resulted as transcriptional activators of other NACs. Moreover, to investigate the common regulative role of the selected NACs on the grapevine transcriptome, all the annotated V. vinifera genes were listed and the most represented genes between all the DAP-seq results were identified. Interestingly, at the top of the ranking we found many genes related to maturation and senescence such as an indole-3-acetic acid-amido synthetase, which could be involved in the establishment and maintenance of low IAA concentrations in ripening berries, a laccase, encoding for a phenylpropanoid pathway-related enzyme, the senescence-inducible chloroplast stay-green protein 1, triggering Chl degradation, and the UTP-glucose-1-phosphate uridylyltransferase, encoding for a carbohydrate-metabolism-related enzyme which is highly expressed in berries at veraison. All these results lay a foundation stone in understanding the genetic regulation of such a complex process as fruit ripening.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Chiara Foresti1*, Alessandra Amato1, Luis Orduña2, Chiara Fattorini1, Erica D’Incà1, Nicola Vitulo1, José Tomás Matus2, Sara Zenoni1

1Department of Biotechnology, University of Verona, Verona, Italy.
2Institute for Integrative Systems Biology (I2SysBio), Universitat de València-CSIC, Valencia, Spain

Contact the author*

Keywords

Berry ripening, cistrome, NAC, hierarchical intra-family network

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Viticulture between adaptation and resilience: the role of the Italian long-term observatories for vineyard energy, water and carbon budgets

Viticulture is exposed to a range of new stressors, that are challenging its sustainability and disrupting famous and well-established production regions. Steady increase of average temperature, recurring heat waves, altered rainfall seasonal distribution, drought spells, increased pathogens pressure, they all mix up with increased frequency, making every growing season a special challenge and calling for new approaches to cope with worrying scenarios.

Ochratoxin a degradation by Botrytis cinerea laccase: effect of oenological factors and redox mediators

This study evaluates the effect of different oenological factors and natural mediators on the degradation of Ochratoxin A (OTA) using Botrytis cinerea laccase.

Mobilizing endogenous transposable elements for grapevine improvement: a genomic and epigenomic approach in New Zealand Sauvignon Blanc

Efforts to improve the New Zealand wine industry’s climate resilience and sustainability through grapevine improvement are limited by germplasm availability and a reliance on Sauvignon Blanc exports. To address this, we are working to generate a population of 12,000 individuals with unique genetic traits, from which to select future clones for major export varieties.

Sauvignon Blanc plantlets are being regenerated from embryogenic callus, using an approach designed to mobilise endogenous transposable elements as mutagens.

Soil Temperature and Climate Change: Implications for Mediterranean Vineyards 

More frequent and extreme temperatures and droughts pose challenges to the wine sector in Mediterranean Europe. Soil is crucial to sustain the equilibrium of ecosystems, economic growth and people’s prosperity worldwide. In viticulture, soils are a major component of the terroir and do influence vine’s growth, yield and berry composition. Soil temperature (ST) affects soil´s physical, chemical and biological processes and also crop growth. The impact of ST becomes even stronger when dealing with row crops such as grapevine, when considering the increased exposition to radiation. However, the impact of ST on crop performance remains poorly described, especially for extreme climatic conditions.

Study of the volatil profile of minority white varieties

The genetic material preservation is a priority issue in winemaking research. The recovery of minority grape varieties can control the genetic erosion, contributing also to preserve wine typical characteristics. In D.O.Ca. Rioja (Spain) the number of grown white varieties has been very limited, representing Viura the 91% of the cultivated white grape area in 2005, while the others, Garnacha Blanca and Malvasía riojana, hardly were grown. For this reason, a recovery and characterization study of plant material was carried out in this region. In 2008, the results obtained allowed the authorization of three minority white varieties: Tempranillo Blanco, Maturana Blanca and Turruntés.