terclim by ICS banner
IVES 9 IVES Conference Series 9 Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Abstract

Variegation, a frequently observed trait in plants, is characterized by the occurrence of white or discoloured plant tissue. This phenomenon is attributed to genetic mosaicism or chimerism, potentially impacting the epidermal (L1) and subepidermal (L2) cell layers. In grapevine, variegation manifests as white or paler leaf, flower, or berry tissues, often leading to stunted growth and impeded development. Despite its prevalence, variegation in grapevines remains understudied. Notably, a natural mutant derived from Sultana, namely Bruce’s Sport, exhibits colour variegation in the leaves, although this occurrence only appears later in the growing season. Conversely, the flowers and berries are always variegated and are paler in colour. Furthermore, studies have observed that Bruce’s Sport displays a lower berry yield compared to the Sultana variety, along with reduced polyphenol oxidase (PPO) activity in the variegated tissues. This study aims to investigate the genetic basis of variegation in Bruce’s Sport and its effects on plant growth and development. To this extent, a transcriptomic analysis was employed comparing data obtained from flower tissue of Sultana and Bruce’s Sport. Additionally, differentially expressed genes were confirmed, aiding in the identification and characterization of genes associated with variegation in the Vitis genome, potentially uncovering candidates for future functional studies.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clara Holm1*, Nina Wiese1, Manuela Campa1, Johan Burger1, Justin Lashbrooke1

1 Genetics Department, Stellenbosch University, South Africa

Contact the author*

Keywords

variegation, grapevine, polyphenol oxidase activity, gene expression

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Acumulación de materia seca, orientada a valorar la fijación de carbono, en función del aporte de riego y la pluviometría, en Cabernet-Sauvignon a lo largo de 15 años

The vineyard is capable of fixing carbon in its permanent structure from atmospheric carbon dioxide, through the process of gas exchange and the performance of photosynthesis. The photosynthetic capacity of the vineyard depends on the water resources that the plant may have at its disposal, so the amount of dry matter, derived from the processed photosynthates, that it can store will depend on the water regime of the crop, both in the annually renewable organs as in permanent parts.

Smoke exposure effects on red wines: how much is too much?

Increasing wildfire frequency in the United States has led to the indirect impact of smoke in vineyards, affecting grape quality and wine sensory attributes, commonly called “smoke taint”.

Etude des effets millésime, situation et sol à partir d’un observatoire du Gamay en beaujolais

Des expérimentations sur Gamay ont été réalisées en Beaujolais de 2000 à 2006 sur 10 parcelles d’AOC différentes. De nombreuses mesures ont été effectuées à différents stades (vigne, baies récoltées, vinification et bouteille avec ou sans vieillissement). Ces mesures sont également de natures différentes (données phénologiques, analytiques, dégustation). Des analyses de la composition des sols sont également disponibles.

Contaminations croisées avec les produits phytosanitaires dans les vins bio. Sources potentielles et mesures de prévention.

Organic wines, although resulting from a production method based on the non-use of synthetic phytosanitary products, are not always free of residues. These residues can result from cross-contamination during production in the field or in the cellar, during the production or aging of the wine. In recent years, with the improvement of analysis techniques, a molecule, phosphonic acid, the main metabolite of fosetyl-al (banned in organic farming) is regularly quantified in organic wines and its origin is not clearly identified.

Launching the GiESCO guide

Launching the GiESCO guide