terclim by ICS banner
IVES 9 IVES Conference Series 9 Phenotypical impact of a floral somatic mutation in the cultivar Listán Prieto

Phenotypical impact of a floral somatic mutation in the cultivar Listán Prieto

Abstract

The accession Criolla Chica Nº2 (CCN2) is catalogued as a floral mutation of cultivar Criolla Chica (synonym for cv. Listán Prieto). Contrary to what is observed in hermaphrodite-cultivated varieties like Criolla Chica, CCN2 exhibits a prevalence of masculinized flowers. Aiming to study the incidence and phenotypical implications of this mutation, CCN2 plants were deeply studied using Criolla Chica ‘Ballista’ (CCBA) as control plants. For each CCN2 plant, two inflorescences per shoot were sampled and segmented into proximal, mid and distal positions, relative to the pedicel. Flowers were observed through magnifying lens and classified according to OIV151 descriptor. CCN2 exhibited flowers of type 1 (masculinized) and 2 (intermediate), while CCBA exhibited only type 3 (hermaphrodite) flowers, as expected. CCN2 averaged more than 55% of type 1 flowers per cluster, which were predominant in the proximal position (63%), gradually diminishing towards distal positions. This distribution correlates with low fruit set rates towards proximal positions. In CCN2, a high percentage of inflorescence abscission per plant (avg. 50%) was observed, starting in stage EL-27. This phenomenon was not observed in CCBA. Additionally, histological sections of flowers at different developmental stages were performed. In type 1 flowers of CCN2, style and stigma tissues exhibited null development with atrophied ovules; these structures were present although poorly developed in type 2 flowers, potentially producing the few berries per cluster observed at harvest. Overall, the studied floral mutation identified in CCN2, strongly affects the development of female reproductive tissues and organs, drastically hindering fruit-set rate and cluster production.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Tomás Oroño1*, Rocío Torres2, Agustín Sanguinetti3, Claudio Muñoz1,4, Sebastián Gomez-Talquenca2, Luciano Calderón1, Diego Lijavetzky1

1Instituto de Biología Agrícola de Mendoza (IBAM, CONICET-UNCuyo), Almirante Brown 500, M5528AHB. Chacras de Coria, Mendoza, Argentina
2EEA Mendoza INTA, San Martin 3853, 5507, Luján de Cuyo, Mendoza, Argentina.
3Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET).
4Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo. Almirante, Brown 500, M5528AHB. Chacras de Coria, Mendoza, Argentina

Contact the author*

Keywords

floral mutation, masculinization, inflorescence abortion, fruit set, histological analysis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Use of multispectral satellite for monitoring vine water status in mediterranean areas

The development of new generations of multispectral satellites such as Sentinel-2 opens possibilities as to vine water status assessment (Cohen et al., 2019). Based on a three years field campaign, a model of Stem Water Potential (SWP) estimation on vine using four satellite bands in Red, Red-Edge, NIR and SWIR domains was developed (Laroche-Pinel et al., 2021). The model relies on SWP field measures done using a pressure chamber (Scholander et al., 1965), which is a common, robust and precise method to assess vine water status (Acevedo-Opazo et al., 2008). The model was mainly developed from from SWP measures on Syrah N (Laroche Pinel E., 2021).

A large scale monitoring was organized in different vineyards in the Mediterranean region in 2021. 10 varieties amongst the most represented in this area were monitored (Cabernet sauvignon N, Chardonnay B, Cinsault N, Grenache N, Merlot N, Mourvèdre N, Sauvignon B, Syrah N, Vermentino B, Viognier B). The model was used to produce water status maps from Sentinel-2 images, starting from the beginning of June (fruit set) up to September (harvest). The average estimated SWP for each vine was compared to actual field SWP measures done by wine growers or technicians during usual monitoring of irrigation programs. The correlations between mean estimated SWP and mean measured SWP were at the same level than expected by the model. (Laroche Pinel, 2021) The general SWP kinetics were comparable. The estimated SWP would have led to same irrigation decisions concerning the date of first irrigation in comparison with measured SWP.

Acevedo-Opazo, C., Tisseyre, B., Ojeda, H., Ortega-Farias, S., Guillaume, S. (2008). Is it possible to assess the spatial variability of vine water status? OENO One, 42(4), 203.
Cohen, Y., Gogumalla, P., Bahat, I., Netzer, Y., Ben-Gal, A., Lenski, I., … Helman, D. (2019). Can time series of multispectral satellite images be used to estimate stem water potential in vineyards? In Precision agriculture ’19, The Netherlands: Wageningen Academic Publishers, pp. 445–451.
Laroche-Pinel, E., Duthoit, S., Albughdadi, M., Costard, A. D., Rousseau, J., Chéret, V., & Clenet, H. (2021). Towards vine water status monitoring on a large scale using sentinel-2 images. remote sensing, 13(9), 1837.
Laroche-Pinel,E. (2021). Suivi du statut hydrique de la vigne par télédétection hyper et multispectrale. Thèse INP Toulouse, France.
Scholander, P.F., Bradstreet, E.D., Hemmingsen, E.A., & Hammel, H.T. (1965). Sap pressure in vascular plants: Negative hydrostatic pressure can be measured in plants. Science, 148(3668), 339–346.

Inactivated yeasts: a case study for the future of precision enology

Yeasts serve as highly versatile tools in oenology. They do more than just perform alcoholic fermentation. Nowadays, yeasts from various species, naturally present in grapes, are selected for specific non-fermentative applications. For example, the use of selected non-saccharomyces at the early stage of winemaking has become a common practice to limit the growth of unwanted microorganisms. When inactivated, yeasts can be fractionated into soluble and insoluble fractions providing a wide range of benefits related to structural components or specific metabolites.

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality.

Application of uv-led in wine as an alternative to sulphur dioxide

Sulfites (SO2) are commonly used in the wine industry to preserve products during storage for antiseptic and antioxidant purposes (Oliveira et al., 2011).

Unraveling grapevine resilience to water and nutrient limitations

Water and nutrient availability significantly impact crop yield, thus the application of sustainable strategies towards efficient water use and nutrient absorption by plants is needed.