terclim by ICS banner
IVES 9 IVES Conference Series 9 Phenotypical impact of a floral somatic mutation in the cultivar Listán Prieto

Phenotypical impact of a floral somatic mutation in the cultivar Listán Prieto

Abstract

The accession Criolla Chica Nº2 (CCN2) is catalogued as a floral mutation of cultivar Criolla Chica (synonym for cv. Listán Prieto). Contrary to what is observed in hermaphrodite-cultivated varieties like Criolla Chica, CCN2 exhibits a prevalence of masculinized flowers. Aiming to study the incidence and phenotypical implications of this mutation, CCN2 plants were deeply studied using Criolla Chica ‘Ballista’ (CCBA) as control plants. For each CCN2 plant, two inflorescences per shoot were sampled and segmented into proximal, mid and distal positions, relative to the pedicel. Flowers were observed through magnifying lens and classified according to OIV151 descriptor. CCN2 exhibited flowers of type 1 (masculinized) and 2 (intermediate), while CCBA exhibited only type 3 (hermaphrodite) flowers, as expected. CCN2 averaged more than 55% of type 1 flowers per cluster, which were predominant in the proximal position (63%), gradually diminishing towards distal positions. This distribution correlates with low fruit set rates towards proximal positions. In CCN2, a high percentage of inflorescence abscission per plant (avg. 50%) was observed, starting in stage EL-27. This phenomenon was not observed in CCBA. Additionally, histological sections of flowers at different developmental stages were performed. In type 1 flowers of CCN2, style and stigma tissues exhibited null development with atrophied ovules; these structures were present although poorly developed in type 2 flowers, potentially producing the few berries per cluster observed at harvest. Overall, the studied floral mutation identified in CCN2, strongly affects the development of female reproductive tissues and organs, drastically hindering fruit-set rate and cluster production.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Tomás Oroño1*, Rocío Torres2, Agustín Sanguinetti3, Claudio Muñoz1,4, Sebastián Gomez-Talquenca2, Luciano Calderón1, Diego Lijavetzky1

1Instituto de Biología Agrícola de Mendoza (IBAM, CONICET-UNCuyo), Almirante Brown 500, M5528AHB. Chacras de Coria, Mendoza, Argentina
2EEA Mendoza INTA, San Martin 3853, 5507, Luján de Cuyo, Mendoza, Argentina.
3Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET).
4Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo. Almirante, Brown 500, M5528AHB. Chacras de Coria, Mendoza, Argentina

Contact the author*

Keywords

floral mutation, masculinization, inflorescence abortion, fruit set, histological analysis

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Characterization of commercial enological tannins and its effect on human saliva diffusion

Commercial oenological tannins (TECs) are widely used in the wine industry. TECs are rich in condensed tannins, hydrolyzable tannins or a mixture of both. Wine grapes are a important source of proanthocyanidins or condensed tannins while oak wood possess a high concentration of hydrolyzable tannins (Obreque-Slier et al., 2009). TECs contribute with the antioxidant capacity of wine, catalyze oxide-reduction reactions and participate in the removal of sulfur compounds and metals.

Different oxygen and sulphur dioxide concentrations in ‘Sauvignon blanc’ must: effect on the composition of the must and wine

The effects of different oxygen and sulphur dioxide additions to South African ‘Sauvignon blanc’ musts were investigated. Oxygen addition without SO2 protection led to lower levels of certain volatile thiols in the wines, with a corresponding decrease in certain phenols and glutathione concentrations.

Wine tannins: What place for grape seed?

Phenolic compounds are among the most important quality factors of wines. They contribute to the organoleptic characteristics of wine such as colour, astringency, and bitterness. Although tannins found in wine can come from microbial and oak sources, the main sources of polyphenols are skin and seed from grapes. Yet, the link between grape seed phenolic content and wine composition, or even the link between seed maturity stage and wine composition are poorly studied. This work describes and explains the seed tannins kinetics release in wine, but also the impact of seed maturity stage on seed tannins extractability.

Estimating bulk stomatal conductance of grapevine canopies

In response to changes in their environment, grapevines regulate transpiration using various physiological mechanisms that alter conductance of water through the soil-plant-atmosphere continuum. Expressed as bulk stomatal conductance at the canopy scale, it varies diurnally in response to changes in vapor pressure deficit and net radiation, and over the season to changes in soil water deficits and hydraulic conductivity of both soil and plant. It is necessary to characterize the response of conductance to these variables to better model how vine transpiration also responds to these variables. Furthermore, to be relevant for vineyard-scale modeling, conductance is best characterized using data collected in a vineyard setting. Applying a crop canopy energy flux model developed by Shuttleworth and Wallace, bulk stomatal conductance was estimated using measurements of individual vine sap flow, temperature and humidity within the vine canopy, and estimates of net radiation absorbed by the vine canopy. These measurements were taken on several vines in a non-irrigated vineyard in Bordeaux France, using equipment that did not interfere with ongoing vineyard operations. An inverted Penman-Monteith equation was then used to calculate bulk stomatal conductance on 15-minute intervals from July to mid-September 2020. Time-series plots show significant diurnal variation and seasonal decreases in conductance, with overall values similar to those in the literature. Global sensitivity analysis using non-parametric regression found transpiration flux and vapor pressure deficit to be the most important input variables to the calculation of bulk stomatal conductance, with absorbed net radiation and bulk boundary layer conductance being much less important. Conversely, bulk stomatal conductance was one of the most important inputs when calculating vine transpiration, further emphasizing the need for characterizing its response to environmental changes for use in vineyard water use modeling.

Contribution to the sensory and volatile characterization of four traditional Galician red varieties

Galicia, a region sited in the northwest of Spain, is one of the most important wine production area, with five Appellations of Origin Controlled (AOC).