terclim by ICS banner
IVES 9 IVES Conference Series 9 Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Abstract

In grape berry pedicel, xylem hydraulic conductance can be impaired by blockage deposition in the lumen of xylem elements. However, the varietal difference of the interruptions has not yet been characterized. In this preliminary work, we utilized synchrotron x-ray computed microtomography experiments performed at MOGNO beamline (LNLS – Brazil) to identify possible blockage sites in natural grape pedicel xylem. For this, we imaged dehydrated pedicel’s stem portion from the Niagara Rosada variety in three different phenological stages (Pre-veraison (PreV), veraison (V) and post-veraison (PostV). The reconstructed tridimensional images with a voxel size of 1.16 µm were segmented for the identification of xylem vessel lumens. After analysing one pedicel stem per stage, we identified 658 vessels without occlusion throughout his axial plane and 41 in which we could identify possible interruptions. The percentage of interrupted vessels was higher at PostV (15,58%) when compared to V (4,09%) and PreV (3,14%). At the same time, the vessels’ volume decreased through berry ripening. This may indicate that a higher hydraulic resistance could advance in Niagara Rosada pedicel during development, reducing water flow to the berry. These findings highlight the importance of tools that allow detailed tridimensional histological analysis of intact tissues. Furthermore, we expect to calculate and understand how the water transport throughout the stem pedicel is affected by the growth/ripening changes in blockages, volume vessels and connections between xylem vessels.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Tainara Perciliano da Silva2, Talita Rosas Ferreira3, Carla Cristina Polo1*

1 Brazilian Synchrotron Light Laboratory (LNLS), CATERETÊ Group
2 Universidade Federal de Alagoas
3 Brazilian Synchrotron Light Laboratory (LNLS), MOGNO Group

Contact the author*

Keywords

vascular occlusions, imaging analysis, grapevine pedicel

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Beyond classical statistics – data fusion coupled with pattern recognition

AIM: Patterns in data obtained from wine chemical and sensory evaluations are difficult to infer using classical statistics.

Banques de données biologiques annuelles par terroir et optimisation des itinéraires culturaux

In addition to studies on the edaphic and landscape characteristics of the environment (Dolédec, 1995), the characterization of the physiology of the vine and of parasitism during its vegetative cycle represents an essential component of knowledge and management of the terroirs.

Study of the impact of nitrogen additions and isothermal temperature on aroma production in oenological fermentation

Nitrogen and temperature are two important factors that influence wine fermentation and volatile compounds production. Among the different compounds present in the must, nitrogen is an essential nutrient for the management of the fermentation kinetics but it also plays an important role in the synthesis of fermentative aromas. To address the problems related to nitrogen deficiencies, nitrogen additions during alcoholic fermentation have been developed.

Influence of vine spacing on water status, productivity, yield and must composition in Tempranillo grapevine under Duero Valley zone conditions

L’objectif de cette étude est analyser l’influence de la densité de plantation sur l’état hydrique (potentiel hydrique), le comportement productif (matière sèche et rendement) et la expression qualitative (poids de baie, degrée Brix, pH, acidité totale, concentration polyphénolique) de la varieté Tempranillo dans la Vallée du Douro, à l’A.O. Cigales.

Can minimal pruning be a strategy to adapt grape ripening to global warming?

Berry maturation in warm areas takes place very early, when temperatures are still high and favorable for carbohydrate synthesis and accumulation in the berries, but not as favorable for maintaining high titratable acidity or low pH, or for increasing berry polyphenol content. Different canopy management techniques have been proven to delay berry maturation at the expense of yield (severe canopy trimming, late spring pruning to induce sprouting of dormant buds, etc.). Minimal pruning delays berry ripening by highly increasing yield and by reducing the leaf area to fruit ratio.