terclim by ICS banner
IVES 9 IVES Conference Series 9 Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Abstract

In grape berry pedicel, xylem hydraulic conductance can be impaired by blockage deposition in the lumen of xylem elements. However, the varietal difference of the interruptions has not yet been characterized. In this preliminary work, we utilized synchrotron x-ray computed microtomography experiments performed at MOGNO beamline (LNLS – Brazil) to identify possible blockage sites in natural grape pedicel xylem. For this, we imaged dehydrated pedicel’s stem portion from the Niagara Rosada variety in three different phenological stages (Pre-veraison (PreV), veraison (V) and post-veraison (PostV). The reconstructed tridimensional images with a voxel size of 1.16 µm were segmented for the identification of xylem vessel lumens. After analysing one pedicel stem per stage, we identified 658 vessels without occlusion throughout his axial plane and 41 in which we could identify possible interruptions. The percentage of interrupted vessels was higher at PostV (15,58%) when compared to V (4,09%) and PreV (3,14%). At the same time, the vessels’ volume decreased through berry ripening. This may indicate that a higher hydraulic resistance could advance in Niagara Rosada pedicel during development, reducing water flow to the berry. These findings highlight the importance of tools that allow detailed tridimensional histological analysis of intact tissues. Furthermore, we expect to calculate and understand how the water transport throughout the stem pedicel is affected by the growth/ripening changes in blockages, volume vessels and connections between xylem vessels.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Tainara Perciliano da Silva2, Talita Rosas Ferreira3, Carla Cristina Polo1*

1 Brazilian Synchrotron Light Laboratory (LNLS), CATERETÊ Group
2 Universidade Federal de Alagoas
3 Brazilian Synchrotron Light Laboratory (LNLS), MOGNO Group

Contact the author*

Keywords

vascular occlusions, imaging analysis, grapevine pedicel

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Viticultural characterisation of soils from triassic period at Beaumes-de-Venise (Côtes du Rhône, France)

Wineries of Beaumes-de-Venise area make their best red wines with grapes from the “Triassic terroir”. This « terroir » is characterized by soils from the Triassic period. These specific soils are complex and quite heterogeneous. They originate from an eventful geological history to keep in mind to understand soils geographical distribution.

Microbial metagenomics of vineyard soils and wine terroir

Aims: The aims of this study were to (i) characterize bacterial and fungal communities in selected Australian vineyard soils and (ii) determine if the soil microbiome composition and diversity varied between different zones within a vineyard. 

A comprehensive study on the effect of foliar mineral treatments on grapevine microbiota, flavonoid gene expression, and berry composition

Recently, foliar treatments with mineral-based compounds have shown positive effects on grapevine production by protecting grape from thermal excesses and reducing the decoupling between technological and phenolic maturity caused by climate change. Unraveling the effect of mineral particle applications on grape-associated microbes is pivotal for successful wine processing, due to the influence of the microbiota on wine composition and stability. To our knowledge, this is the first work that comprehensively studied the effects of kaolin and chabasite-rich zeolitites treatments on grape-related microorganisms (by real-time PCR quantification of total fungi, Hanseniospora uvarum, Metschnikowia pulcherrima, plant-associated bacteria and lactic acid bacteria), the expression of genes related to the flavonoid biosynthesis (PAL1, CHS1, F3H2, DFR, LDOX, UFGT, MYBA1, GST4, FLS4 genes) and the berry composition (°Brix, pH, acidity and anthocyanin concentrations) in cv. Sangiovese during ripening in two growing seasons (2019 and 2020).

Approaches to the classification of wine aroma aging potential. Applications to the case of Valpolicella red wines

Unlike most of other foods, wine sensory quality is thought to reach a peak after an aging period. In the case of the Valpolicella red wines

Determination of metallic elements in Chilean wines by atomic absorption spectroscopy and inductively coupled plasma–mass spectrometry

The chemical composition of wines depends on series of variables such as the type of grape, edaphoclimatic conditions, and viticulture and winemaking practices employed during production. Metallic elements play a significant role during winemaking (e.g. as catalysts of oxidation reactions) and have been previously employed for the classification of wines according to provenance. In this work, we focused on the analysis of metallic elements (K, Na, Ca, Zn, Cu, Fe, Mg, Mn, Ni, Cr, Al, Pb, Cd, Hg, Se, Co, Sn and As) in 145 Chilean wine samples (102 reds and 43 white wines), of seven grape varieties, and five of the major wine producing regions in Chile.