terclim by ICS banner
IVES 9 IVES Conference Series 9 Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Abstract

In grape berry pedicel, xylem hydraulic conductance can be impaired by blockage deposition in the lumen of xylem elements. However, the varietal difference of the interruptions has not yet been characterized. In this preliminary work, we utilized synchrotron x-ray computed microtomography experiments performed at MOGNO beamline (LNLS – Brazil) to identify possible blockage sites in natural grape pedicel xylem. For this, we imaged dehydrated pedicel’s stem portion from the Niagara Rosada variety in three different phenological stages (Pre-veraison (PreV), veraison (V) and post-veraison (PostV). The reconstructed tridimensional images with a voxel size of 1.16 µm were segmented for the identification of xylem vessel lumens. After analysing one pedicel stem per stage, we identified 658 vessels without occlusion throughout his axial plane and 41 in which we could identify possible interruptions. The percentage of interrupted vessels was higher at PostV (15,58%) when compared to V (4,09%) and PreV (3,14%). At the same time, the vessels’ volume decreased through berry ripening. This may indicate that a higher hydraulic resistance could advance in Niagara Rosada pedicel during development, reducing water flow to the berry. These findings highlight the importance of tools that allow detailed tridimensional histological analysis of intact tissues. Furthermore, we expect to calculate and understand how the water transport throughout the stem pedicel is affected by the growth/ripening changes in blockages, volume vessels and connections between xylem vessels.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Tainara Perciliano da Silva2, Talita Rosas Ferreira3, Carla Cristina Polo1*

1 Brazilian Synchrotron Light Laboratory (LNLS), CATERETÊ Group
2 Universidade Federal de Alagoas
3 Brazilian Synchrotron Light Laboratory (LNLS), MOGNO Group

Contact the author*

Keywords

vascular occlusions, imaging analysis, grapevine pedicel

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Cold plasma at atmospheric pressure for eliminating Brettanomyces from oak wood

In the oenological industry, the maintenance and sanitation of oak barrels has become a fundamental task. The wood has a porous structure that facilitates the penetration not only of the wine, but of the microorganisms it contains, such as the alterative yeast Brettanomyces bruxellensis.

Read More

Determination of aromatic characteristics from Syrah and Tempranillo tropical wines elaborated in Northeast Brazil

Dans la region Nord-Est du Brésil, située à la Vallée du São Francisco, localiséee entre les paralleles 8-9º HS, la production de vins tropicaux a commencé il y a une vigntaine d’années. Dans cette région, il est possible d’avoir au minimum deux récoltes par an, car la moyenne de température est de 26 ºC, avec une pluviosité moyenne de 550 mm entre les mois de janvier-avril.

Read More

Somatic embryogenesis and organogenesis: driving regeneration forces behind grapevine genetic transformation

Cell pluripotency, enables the possibility to change the cellular fate, stimulating the reorganization and the formation of new vegetative structures from differentiated somatic tissues. Although several factors are implicated in determining the success of a breeding program through the use of modern biotechnological techniques, the definition of a specific regeneration strategy is fundamental to speed up and make these applications feasible.

Read More

Comparing the effects of vision, smell and taste in red wine quality judgments by experts: sensory cues, mental imagery and verbal representations as drivers of consensus in the multisensory space

In this study, we evaluated the contributions of vision, smell and taste to red wine quality judgments by expert wine tasters.

Read More

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Read More