terclim by ICS banner
IVES 9 IVES Conference Series 9 Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Abstract

In grape berry pedicel, xylem hydraulic conductance can be impaired by blockage deposition in the lumen of xylem elements. However, the varietal difference of the interruptions has not yet been characterized. In this preliminary work, we utilized synchrotron x-ray computed microtomography experiments performed at MOGNO beamline (LNLS – Brazil) to identify possible blockage sites in natural grape pedicel xylem. For this, we imaged dehydrated pedicel’s stem portion from the Niagara Rosada variety in three different phenological stages (Pre-veraison (PreV), veraison (V) and post-veraison (PostV). The reconstructed tridimensional images with a voxel size of 1.16 µm were segmented for the identification of xylem vessel lumens. After analysing one pedicel stem per stage, we identified 658 vessels without occlusion throughout his axial plane and 41 in which we could identify possible interruptions. The percentage of interrupted vessels was higher at PostV (15,58%) when compared to V (4,09%) and PreV (3,14%). At the same time, the vessels’ volume decreased through berry ripening. This may indicate that a higher hydraulic resistance could advance in Niagara Rosada pedicel during development, reducing water flow to the berry. These findings highlight the importance of tools that allow detailed tridimensional histological analysis of intact tissues. Furthermore, we expect to calculate and understand how the water transport throughout the stem pedicel is affected by the growth/ripening changes in blockages, volume vessels and connections between xylem vessels.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Tainara Perciliano da Silva2, Talita Rosas Ferreira3, Carla Cristina Polo1*

1 Brazilian Synchrotron Light Laboratory (LNLS), CATERETÊ Group
2 Universidade Federal de Alagoas
3 Brazilian Synchrotron Light Laboratory (LNLS), MOGNO Group

Contact the author*

Keywords

vascular occlusions, imaging analysis, grapevine pedicel

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sustaining wine identity through intra-varietal diversification

With contemporary climate change, cultivated Vitis vinifera L. is at risk as climate is a critical component in defining ecologically fitted plant materiel. While winegrowers can draw on the rich diversity among grapevine varieties to limit expected impacts (Morales-Castilla et al., 2020), replacing a signature variety that has created a sense of local distinctiveness may lead to several challenges. In order to sustain wine identity in uncertain climate outcomes, the study of intra-varietal diversity is important to reflect the adaptive and evolutionary potential of current cultivated varieties. The aim of this ongoing study is to understand to what extent can intra-varietal diversity be a climate change adaptation solution. With a focus on early (Sauvignon blanc, Riesling, Grolleau, Pinot noir) to moderate late (Chenin, Petit Verdot, Cabernet franc) ripening varieties, data was collected for flowering and veraison for the various studied accessions (from conservatory plots) and clones. For these phenological growing stages, heat requirements were established using nearby weather stations (adapted from the GFV model, Parker et al., 2013) and model performances were verified. Climate change projections were then integrated to predict the future behaviour of the intra-varietal diversity. Study findings highlight the strong phenotypic diversity of studied varieties and the importance of diversification to enhance climate change resilience. While model performances may require improvements, this study is the first step towards quantifying heat requirements of different clones and how they can provide adaptation solutions for winegrowers to sustain local wine identity in a global changing climate. As genetic diversity is an ongoing process through point mutations and epigenetic adaptations, perspective work is to explore clonal data from a wide variety of geographic locations.

A research agenda for terroir: an empirical, international expert study

Aim: Terroir is a French concept relating the qualities and quality of agricultural products to their physical and socio-cultural place of origin. It is increasingly used by business and policymakers as a marketing technique to provide economic benefits (e.g. Lenglet, 2014; Wine Australia, 2015), and to potentially preserve cultural heritage (e.g. Bauer, 2009) and the environment (e.g. Bowen, 2010)

Diversity of leaf functioning under water deficit in a large grapevine panel: high throughput phenotyping and genetic analyses

Water resource is a major limiting factor impacted by climate change that threatens grapevine production and quality. Understanding the ecophysiological mechanisms involved in the response to water deficit is crucial to select new varieties more drought tolerant. A major bottleneck that hampers such advances is the lack of methods for measuring fine functioning traits on thousands of plants as required for genetic analyses. This study aimed at investigating how water deficit affects the trade-off between carbon gains and water losses in a large panel representative of the Vitis vinifera genetic diversity. 250 genotypes were grown under 3 watering scenarios (well-watered, moderate and severe water deficit) in a high-throughput phenotyping platform.

Consumers’ emotional responses elicited by wines according to organoleptic quality

Wine is often described with emotional terms, such as surprising, disappointing or pleasant. However, very little has been done to really characterize this link between emotions and wine. Can it really bring emotions to wine tasters? Many studies have looked at the extrinsic factors that can improve the emotional

Measurement of trans-membrane and trans-tissue voltages in the Shiraz berry mesocarp

In mid to late ripening, sugar and potassium (K+) accumulation into the berry slows and is eventually completed1. K+ is the most abundant cation in the berry, undertaking important physiological roles.