terclim by ICS banner
IVES 9 IVES Conference Series 9 Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Xylem vessel blockages in grape pedicel growing in tropical climate observed by microtomography

Abstract

In grape berry pedicel, xylem hydraulic conductance can be impaired by blockage deposition in the lumen of xylem elements. However, the varietal difference of the interruptions has not yet been characterized. In this preliminary work, we utilized synchrotron x-ray computed microtomography experiments performed at MOGNO beamline (LNLS – Brazil) to identify possible blockage sites in natural grape pedicel xylem. For this, we imaged dehydrated pedicel’s stem portion from the Niagara Rosada variety in three different phenological stages (Pre-veraison (PreV), veraison (V) and post-veraison (PostV). The reconstructed tridimensional images with a voxel size of 1.16 µm were segmented for the identification of xylem vessel lumens. After analysing one pedicel stem per stage, we identified 658 vessels without occlusion throughout his axial plane and 41 in which we could identify possible interruptions. The percentage of interrupted vessels was higher at PostV (15,58%) when compared to V (4,09%) and PreV (3,14%). At the same time, the vessels’ volume decreased through berry ripening. This may indicate that a higher hydraulic resistance could advance in Niagara Rosada pedicel during development, reducing water flow to the berry. These findings highlight the importance of tools that allow detailed tridimensional histological analysis of intact tissues. Furthermore, we expect to calculate and understand how the water transport throughout the stem pedicel is affected by the growth/ripening changes in blockages, volume vessels and connections between xylem vessels.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Eduardo Monteiro1, Tainara Perciliano da Silva2, Talita Rosas Ferreira3, Carla Cristina Polo1*

1 Brazilian Synchrotron Light Laboratory (LNLS), CATERETÊ Group
2 Universidade Federal de Alagoas
3 Brazilian Synchrotron Light Laboratory (LNLS), MOGNO Group

Contact the author*

Keywords

vascular occlusions, imaging analysis, grapevine pedicel

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Image based vineyard yield prediction using empirical models to estimate bunch occlusion by leaves

Vineyard yield estimation brings several advantages to the entire wine industry. It can provide useful information to support decision making regarding bunch thinning practices, harvest logistics and marketing strategies, as well as to manage stored wine and cellar tanks allocation. Today, this estimation is performed mainly using manual methods based on destructive bunch sampling.

Development of spectral indices to monitoring non-destructive of ripeness for water stressed grapevine (Vitis vinifera L.) using contour map optimization

Accurate and non-destructive monitoring of grape ripening is essential for optimising harvest decisions, particularly under water stress conditions.

Winter physiology in a warmer world: Cold hardiness and deacclimation sensitivity drive variation in spring phenology

As the climate warms, the focus of concern in viticulture often turns to how higher temperatures may shift growing regions, change the character of AVAs, and alter fruit quality. However, climate warming is increasing most quickly during the winter dormancy cycle, a critical and often underappreciated portion of the grapevine life cycle. In response to decreasing temperatures and decreasing daylength, grapes initiate a series of physiological changes to enter dormancy, acquire freeze resistance, and time spring phenology such that the growing season begins after threat of frost.

Towards an ecological architecture inspired by underground cellars: An example of the thermal inertia of Moldovan underground cellars and new geothermal and Canadian well approaches

The search for underground shelters is one of the oldest forms of human habitation, providing refuge in extreme environments such as deserts and polar regions.

Combination of NIR multispectral information acquired from a ground moving vehicle with AI methods to assess the vine water status in a Tempranillo (Vitis vinifera L.) commercial vineyard

Increasing water scarcity and unpredictable rainfall patterns necessitate efficient water management in grape production. This study proposes a novel approach for monitoring grapevine water status in a commercial vertically-shoot-positioned Vitis vinifera L. Tempranillo vineyard using non-invasive spectroscopy with a battery of different AI methods to assess vineyard water status, that could drive precise irrigation. A contactless, miniature NIR spectrometer (900-1900 nm) mounted on a moving vehicle (3 Km/h) was employed to collect spectral data from the vines’ northeast side along six dates in season 2021.