terclim by ICS banner
IVES 9 IVES Conference Series 9 Genome wide association mapping of phenology related traits in Vitis vinifera L

Genome wide association mapping of phenology related traits in Vitis vinifera L

Abstract

Climate change, with rise in temperatures, is leading to an advance in the dates of phenological stages, with a loss in quality of the grape final product. Therefore, the understanding of the genetic determinants driving the phenological stages of flowering, veraison and the interval between them, represents a target for the development of grapevine’s cultivar adapted to the changing environment.
Here we conducted a GWA study to identify SNPs significantly associated to flowering time, veraison time and to the interval among them. A germplasm collection (CREA-VE in Susegana, Treviso, Italy) including 649 grapevine’s cultivar representing 365 unique genotypes was considered. Cultivars were phenotyped for flowering time and veraison time along 11 years. Flowering-veraison intervals were also derived and distribution for all traits was inspected and eventually corrected. For this analysis we have built a genetic dataset including 6679 SNPs. SNPs were either recovered from litterature or integrated by genotyping through grapevine Illumina SNPChip 18K and used for evaluating the genetic structure. MLM analysis conducted independently for the three different phenological traits identified a list of few significantly associated SNPs. Among the three traits flowering time yielded the highest number of associated SNPs. For each trait SNPs consistently associated across more years were found. Moreover partially overlapping SNPs associated both to veraison time and flowering-veraison time interval were found. Interestingly most of the associated SNPs co-localized with QTL regions already known either for flowering or veraison traits in grapevine. Putative candidate genes underlying such regions are discussed.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Giada Bolognesi1, Pietro Delfino1, Chiara Broccanello1, Riccardo Mora1, Martina Marini1, 2, Massimo Gardiman2, Mirella Giust2, Diego Tomasi2, Manna Crespan2, Diana Bellin1*

1Department of Biotechnology, University of Verona, Verona, Italy
2 CREA Research Centre for Viticulture and Enology, Conegliano (TV), Italy

Contact the author*

Keywords

Climate change, GWAS, phenology, candidate genes

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

Characterizing chemical influences of smoke on wine via novel application of 13c-labelled smoke

Smoke impact is an ongoing and growing issue for vintners across the globe, with the west coast of the U.S. and Australia being two of the largest wine industries impacted. Wine has shown to be especially sensitive to smoke exposure, often acquiring off-flavor sensory characteristics, such as “burnt rubber”, “ashy”, or other medicinal off-flavors.1 While several studies have examined the chemical composition of smoke influences on wine, some studies disagree on what compounds are having the largest impact on smell and flavor.2 This study is designed as a bottom-up approach to inventory the chemical compounds derived from smoke from a grassland-like fire that are potentially influencing wine chemical composition.

Valorisation of by-product-derived polysaccharides as a sustainable strategy to improve the sensory profile of wine

The agri-food industry generates large amounts of coproducts and by-products that represent valuable resources for developing sustainable and innovative solutions.

Research on the origin and the side effects of chitosan stabilizing properties in wine

Fungal chitosan is a polysaccharide made up of glucosamine and N-acetyl-glucosamine and derived from chitin-glucan of Aspergillus niger or Agaricus bisporus. Fungal chitosan has been authorized as an antiseptic agent in wine since 2009 (OIV) and in organic wine in 2018. At the maximum dose of 10g/hl, it was shown to eliminate Brettanomyces bruxellensis, the main spoilage agent in red wines. Fungal chitosan is highly renewable, biocompatible (ADI equivalent to sucrose) and non-allergenic. However, winemakers often prefer to use sulfites (SO2), though sulfites are classified as priority food allergens, than chitosan. Indeed, many conflicting reports exist regarding its efficiency and its side effects towards beneficial wine microorganisms or wine taste. These contradictions could be explained by the heterogeneity of the fungal chitosan lots traded, the diversity of the wines (chemical composition, winemaking process), but also, by the recently highlighted huge genetic diversity prevailing in wine microbial species.