terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterizing graft union formation in different scion/rootstock combinations of grapevine 

Characterizing graft union formation in different scion/rootstock combinations of grapevine 

Abstract

In most viticultural regions, grapevines are cultivated grafted, employing either hybrid or pure species of various American Vitis spp., such as V. berlandieri, V. rupestris, and V. riparia, as grapevine rootstocks. These rootstocks play a crucial role in providing resistance to the Phylloxera insect pest. Beyond Phylloxera resistance, it is desirable for grapevine rootstocks to exhibit resistance to other soil-borne pathogens and adaptability to abiotic stress conditions. The introduction of new rootstocks holds promise for adapting agriculture to climate change without altering the characteristics of the final harvested product. However, achieving high success rates in grafting for new rootstock genotypes is imperative. This study aims to develop quantitative techniques for characterizing graft union formation in different grapevine scion/rootstock combinations. The research focuses on the initial months after grafting, examining factors such as the quantity of callus (both fresh and dry mass) and the mechanical strength of the graft union. Interestingly, the quantity of callus at the graft interface varied among genotypes and did not necessarily correlate with the mechanical strength of the graft union. Challenges in quantitatively phenotyping different stages of graft union formation have impeded the identification of genetic determinants for grafting success across plant species. To address this bottleneck, various quantitative techniques are being developed to elucidate the genetic architecture of graft union formation in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marilou Camboué1, Jean-Pascal Tandonnet1, Marine Morel1, Elisa Marguerit1, Sarah Jane Cookson1*

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882, Villenave d’Ornon, France

Contact the author*

Keywords

scion, rootstock, grafting, callus, mechanical strength

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Identification of riboflavin low producer yeasts to prevent the light-struck taste in white wines

Wine quality maintenance during the storage is a fundamental aspect for both wine producers and consumers. Nowadays, great attention has been given to the light effect

Investigation of the biostimulant activity of naringenin on anthocyanins biosynthesis: from an explanatory transcriptomic approach on Gamay callus towards a future vineyard application

Context and purpose of the study. Anthocyanins are essential phenolic compounds in red wine, contributing significantly to colour intensity, stability, and sensory quality.

Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Grafting plants uses intrinsic healing processes to join two different plants together to create one functional organism. To further our understanding of the molecular changes occurring during graft union formation in grapevine, we characterized the metabolome and transcriptome of intact and wounded cuttings (with and without buds to represent scions and rootstocks respectively), and homo- and heterografts at 0 and 14 days after wounding/grafting. As over-wintering, dormant plant material was grafted, we also characterized the gene expression changes in the wood during bud burst and spring activation of growth. We observed an asymmetrical pattern of gene expression between above and below the graft interface, auxin and sugar related genes were up-regulated above the graft interface, while genes involved in stress responses were up-regulated below the graft interface.

Assessment of plant water consumption rates under climate change conditions through an automated modular platform

The impact of climate change is noticeable in the present weather, making water scarcity the most immediate mediator reducing the performance and viability of crops, including grapevine (Vitis vinifera L.). The present study developed a system (hardware, firmware, and software) for the determination of plant water use through changes in weight through a period. The aim is to measure the differences in grapevine water consumption in response to climate change (+4oC and 700 ppm) under controlled conditions. The results reveal a correlation between daily plant consumption rates and reference evapotranspiration (ETo).

Wine racking in the winery and the use of inerting gases

The O2 uptake in the different winemaking processes is generally considered to be negative for the sensory characteristics of white and rosé wines. Wine racking is a critical point of O2 uptake, as the large surface area of the wine exposed during this operation and the inability to maintain an effective inert gas blanket over it.
The objective was to study O2 uptake during the racking of a model wine without using inert gases and to compare it with the purging of the destination tank with different inert gases.