terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterizing graft union formation in different scion/rootstock combinations of grapevine 

Characterizing graft union formation in different scion/rootstock combinations of grapevine 

Abstract

In most viticultural regions, grapevines are cultivated grafted, employing either hybrid or pure species of various American Vitis spp., such as V. berlandieri, V. rupestris, and V. riparia, as grapevine rootstocks. These rootstocks play a crucial role in providing resistance to the Phylloxera insect pest. Beyond Phylloxera resistance, it is desirable for grapevine rootstocks to exhibit resistance to other soil-borne pathogens and adaptability to abiotic stress conditions. The introduction of new rootstocks holds promise for adapting agriculture to climate change without altering the characteristics of the final harvested product. However, achieving high success rates in grafting for new rootstock genotypes is imperative. This study aims to develop quantitative techniques for characterizing graft union formation in different grapevine scion/rootstock combinations. The research focuses on the initial months after grafting, examining factors such as the quantity of callus (both fresh and dry mass) and the mechanical strength of the graft union. Interestingly, the quantity of callus at the graft interface varied among genotypes and did not necessarily correlate with the mechanical strength of the graft union. Challenges in quantitatively phenotyping different stages of graft union formation have impeded the identification of genetic determinants for grafting success across plant species. To address this bottleneck, various quantitative techniques are being developed to elucidate the genetic architecture of graft union formation in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marilou Camboué1, Jean-Pascal Tandonnet1, Marine Morel1, Elisa Marguerit1, Sarah Jane Cookson1*

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882, Villenave d’Ornon, France

Contact the author*

Keywords

scion, rootstock, grafting, callus, mechanical strength

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Evaluating Smoke Contaminants in Wine Using 13C-Labelled Barley as a Fuel Source

Wildfires are becoming more common in many areas of the world that are also associated with wine grape production, especially the Pacific northwest United States, Australia and even some areas of France.

Under-row low competitive herbaceous cover: A sustainable alternative to herbicide in vineyards

Weeds are undesirable plants in agroecosystems as they compete with the crop for essential resources such as light, water and nutrients, compromising the final yield and its quality.

Effect of elicitors and ripening moment on the phenolic composition of Monastrell

Grapevine (Vitis vinifera L.) is a globally cultivated crop and economically significant, particularly in the wine industry (Varela et al., 2024). Climate change is already affecting vineyards and is expected to worsen (Averbeck et al., 2019; Dupuis and Knoepfel, 2011).

L’étude “terroirs d’Anjou”: un exemple de caractérisation intégrée des terroirs viticoles, utilisable à l’échelle parcellaire

Natural factors of the production (“terroir” and vintage) are known as an important element for identifying wines by their genuine typicité and their authenticity. The program “Terroirs d’Anjou” (1994-1999) aims at bringing the necessary scientific basis for a rational and reasoned exploitation of the terroir.

Estimating bulk stomatal conductance of grapevine canopies

In response to changes in their environment, grapevines regulate transpiration using various physiological mechanisms that alter conductance of water through the soil-plant-atmosphere continuum. Expressed as bulk stomatal conductance at the canopy scale, it varies diurnally in response to changes in vapor pressure deficit and net radiation, and over the season to changes in soil water deficits and hydraulic conductivity of both soil and plant. It is necessary to characterize the response of conductance to these variables to better model how vine transpiration also responds to these variables. Furthermore, to be relevant for vineyard-scale modeling, conductance is best characterized using data collected in a vineyard setting. Applying a crop canopy energy flux model developed by Shuttleworth and Wallace, bulk stomatal conductance was estimated using measurements of individual vine sap flow, temperature and humidity within the vine canopy, and estimates of net radiation absorbed by the vine canopy. These measurements were taken on several vines in a non-irrigated vineyard in Bordeaux France, using equipment that did not interfere with ongoing vineyard operations. An inverted Penman-Monteith equation was then used to calculate bulk stomatal conductance on 15-minute intervals from July to mid-September 2020. Time-series plots show significant diurnal variation and seasonal decreases in conductance, with overall values similar to those in the literature. Global sensitivity analysis using non-parametric regression found transpiration flux and vapor pressure deficit to be the most important input variables to the calculation of bulk stomatal conductance, with absorbed net radiation and bulk boundary layer conductance being much less important. Conversely, bulk stomatal conductance was one of the most important inputs when calculating vine transpiration, further emphasizing the need for characterizing its response to environmental changes for use in vineyard water use modeling.