terclim by ICS banner
IVES 9 IVES Conference Series 9 Characterizing graft union formation in different scion/rootstock combinations of grapevine 

Characterizing graft union formation in different scion/rootstock combinations of grapevine 

Abstract

In most viticultural regions, grapevines are cultivated grafted, employing either hybrid or pure species of various American Vitis spp., such as V. berlandieri, V. rupestris, and V. riparia, as grapevine rootstocks. These rootstocks play a crucial role in providing resistance to the Phylloxera insect pest. Beyond Phylloxera resistance, it is desirable for grapevine rootstocks to exhibit resistance to other soil-borne pathogens and adaptability to abiotic stress conditions. The introduction of new rootstocks holds promise for adapting agriculture to climate change without altering the characteristics of the final harvested product. However, achieving high success rates in grafting for new rootstock genotypes is imperative. This study aims to develop quantitative techniques for characterizing graft union formation in different grapevine scion/rootstock combinations. The research focuses on the initial months after grafting, examining factors such as the quantity of callus (both fresh and dry mass) and the mechanical strength of the graft union. Interestingly, the quantity of callus at the graft interface varied among genotypes and did not necessarily correlate with the mechanical strength of the graft union. Challenges in quantitatively phenotyping different stages of graft union formation have impeded the identification of genetic determinants for grafting success across plant species. To address this bottleneck, various quantitative techniques are being developed to elucidate the genetic architecture of graft union formation in grapevine.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Marilou Camboué1, Jean-Pascal Tandonnet1, Marine Morel1, Elisa Marguerit1, Sarah Jane Cookson1*

1 EGFV, Univ. Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, F-33882, Villenave d’Ornon, France

Contact the author*

Keywords

scion, rootstock, grafting, callus, mechanical strength

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Multidisciplinary strategies for understanding ill-defined concepts

Aims: The objective of the present work is to review strategies applied to decrypt multidimensional and ill-defined concepts employed by winemakers and to illustrate these strategies with recent applications.

From plant water status to wine flavonoid composition: a precision viticulture approach in a Sonoma county vineyard

Plant water status of grapevine plays a critical role in affecting berry and final wine chemical composition. The environmental variabilities existing in vineyard system have significant impacts on plant water status, but it is challenging to individualize environmental factors from the temporal and spatial variabilities in vineyard. Therefore, there is need to monitor the ecophysical variation through utilizing precision viticulture tools in order to minimize the separation in berry composition. This study aims at delineating vineyard into different management zones based on plant water status explained by soil texture, and utilize differential harvest to equilibrate the final berry and wine composition.

Regulation of terpene production in methyl jasmonate treated cell-cultures

Terpenes are responsible for flavors and aromas of grapes, however, they also protect from radiation, participate in biotic stress and antioxidant mechanisms. The phytohormone methyl jasmonate (MeJA) mediates many of these stress responses and has been associated with increased terpene content in berries. Here, we generated transcriptomic data of Vitis vinifera cv. ‘Gamay’ cells treated with MeJA (100 μM) and cyclodextrins (50 μM) to understand these responses. Ontology analysis revealed that up-regulated genes (URGs) were enriched in jasmonic acid biosynthesis and signaling terms, as expected. Inspection of transcription factors (TFs) among URGs allowed us to study uncharacterized TFs.

Exploring the influence of grapevine rootstock on yield components 

Yield is an agronomic trait that is critical to the sustained success and profitability of the wine industry. In the context of global warming, overall yield tends to decrease. Rootstock has been identified as a relevant lever for adaptation to changing environmental conditions. The aims of this study are; i) to finely identify the components of the yield influenced by rootstock; ii) to characterise the rootstock × scion interaction; iii) to understand the trade-off between vigour and yield.

EFFECTS OF WINEMAKING FACTORS AND AGEING ON THE POLYPHENOLIC AND COLORIMETRIC PROFILES IN RED WINES PRONE TO COLOUR INSTABILITY

The effects of (A) grape freezing, and (B) malolactic fermentation, have been evaluated on the chemical and colorimetric profiles of red wines from Schiava grossa cv. grapes, thus prone to colour instability. The aim was to observe if specific variables (e.g. grape freezing) could improve the extraction and stability of pigments. The samples were studied from musts up to twelve months in bottle. The study was conducted with independent parallel micro-vinifications (12 = 4 theses x 3 replicates) under strictly-controlled conditions.