terclim by ICS banner
IVES 9 IVES Conference Series 9 Grape development revisited through the single-berry metabolomic clock paradigm

Grape development revisited through the single-berry metabolomic clock paradigm

Abstract

Although the ripening process of grapevine berries is well-documented at the vineyard level, pinpointing distinct developmental stages remains challenging. The asynchronous development of berries results in dynamic biases and metabolic chimerism. It is thus crucial to consider individual berries separately and resynchronize their internal clock for deciphering physiological changes throughout development. Given the importance of grape composition in wine quality, we aimed at measuring developmental changes in the metabolome of Syrah single berries from anthesis to over-ripening, without a priori preconceived. Non-targeted UHPLC-HRMS analyses of single berries yielded 9,335 compounds with specific mass and retention time. This dataset was submitted to an analysis workflow, combining classification and dimension reduction tools, to reveal the dynamics of metabolite composition. The outcomes of this workflow highlight an innovative redefinition of developmental stages, through the clustering of metabolites into 11 specific kinetic patterns. More precisely, the usual double sigmoidal growth pattern could be split into more transient stages characterized by the accumulation of specific metabolites. For instance, we identified a cluster of metabolites annunciative of the onset of ripening at the end of the herbaceous plateau which witnesses transient lipidic changes. We also found a cluster composed of stilbenes that accumulate during berry shriveling, following sugar loading. This non-targeted approach enables a more precise and unbiased characterization of grapevine berry development through the metabolomic clock paradigm, paving the way for a better assessment of berry physiological stage in genetic studies and ultimately for varietal selection and adaptation to climate change.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Flora Tavernier1*, Stefania Savoi2, Laurent Torregrosa3, Philippe Hugueney4, Raymonde Baltenweck4, Vincent Segura1* 5and Charles Romieu1 5

1 UMR AGAP Institute, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34398 Montpellier, France
2 Department of Agricultural, Forest and Food Sciences, Università di Torino (UniTO), 10095 Grugliasco, Italy
3 UMR LEPSE, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34060 Montpellier, France
4 UMR SVQV, INRAe-Colmar, 68000 Colmar, France
5 UMT Geno-Vigne, IFV-INRAe-Institut Agro Montpellier, 34398 Montpellier, France

Contact the author*

Keywords

Vitis vinifera L., untargeted metabolites, single berry, phenology

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Transcriptomic and metabolomic responses to wounding and grafting in grapevine

Grafting plants uses intrinsic healing processes to join two different plants together to create one functional organism. To further our understanding of the molecular changes occurring during graft union formation in grapevine, we characterized the metabolome and transcriptome of intact and wounded cuttings (with and without buds to represent scions and rootstocks respectively), and homo- and heterografts at 0 and 14 days after wounding/grafting. As over-wintering, dormant plant material was grafted, we also characterized the gene expression changes in the wood during bud burst and spring activation of growth. We observed an asymmetrical pattern of gene expression between above and below the graft interface, auxin and sugar related genes were up-regulated above the graft interface, while genes involved in stress responses were up-regulated below the graft interface.

Read More

Methyljasmonate versus nanomethyljasmonate: effect on monastrell nitrogen composition

The aim of this work was to evaluate the effect of preharvest application in Monastrell berries using two different types of applications: conventional treatments

Read More

Les motivations du vigneron en quête de l’expression “terroir”

During the 1985 harvest, I was able to notice in the taste perception a break in the harmony of the wine during even partial blends of grapes from different plots. At the same time, I noted a good reaction from customers for greater product customization. As a result, I was led to seek the objective limits of the terroir of a cuvée and by a constant and permanent refinement of the parameters specific to each of the terroirs.

Read More

Determination of metallic elements in Chilean wines by atomic absorption spectroscopy and inductively coupled plasma–mass spectrometry

The chemical composition of wines depends on series of variables such as the type of grape, edaphoclimatic conditions, and viticulture and winemaking practices employed during production. Metallic elements play a significant role during winemaking (e.g. as catalysts of oxidation reactions) and have been previously employed for the classification of wines according to provenance. In this work, we focused on the analysis of metallic elements (K, Na, Ca, Zn, Cu, Fe, Mg, Mn, Ni, Cr, Al, Pb, Cd, Hg, Se, Co, Sn and As) in 145 Chilean wine samples (102 reds and 43 white wines), of seven grape varieties, and five of the major wine producing regions in Chile.

Read More

Development of a new sustainable filtering media for wine and beer clarification and sterilisation

Different separation techniques are frequently used during vinification process. Nowadays, clarification and microbiological stabilization of wine or beer can be done using precoat filters or crossflow filters to remove yeast and bacteria. Kieselguhr powders are the most used filter aids for precoat filtration. Their crystalline structure and their pulverulent nature induce ecotoxicological risks when used. Moreover, regeneration and reuse of these filter aids is not efficient and the filtration waste requires cost effective retreatment.

Read More