terclim by ICS banner
IVES 9 IVES Conference Series 9 Grape development revisited through the single-berry metabolomic clock paradigm

Grape development revisited through the single-berry metabolomic clock paradigm

Abstract

Although the ripening process of grapevine berries is well-documented at the vineyard level, pinpointing distinct developmental stages remains challenging. The asynchronous development of berries results in dynamic biases and metabolic chimerism. It is thus crucial to consider individual berries separately and resynchronize their internal clock for deciphering physiological changes throughout development. Given the importance of grape composition in wine quality, we aimed at measuring developmental changes in the metabolome of Syrah single berries from anthesis to over-ripening, without a priori preconceived. Non-targeted UHPLC-HRMS analyses of single berries yielded 9,335 compounds with specific mass and retention time. This dataset was submitted to an analysis workflow, combining classification and dimension reduction tools, to reveal the dynamics of metabolite composition. The outcomes of this workflow highlight an innovative redefinition of developmental stages, through the clustering of metabolites into 11 specific kinetic patterns. More precisely, the usual double sigmoidal growth pattern could be split into more transient stages characterized by the accumulation of specific metabolites. For instance, we identified a cluster of metabolites annunciative of the onset of ripening at the end of the herbaceous plateau which witnesses transient lipidic changes. We also found a cluster composed of stilbenes that accumulate during berry shriveling, following sugar loading. This non-targeted approach enables a more precise and unbiased characterization of grapevine berry development through the metabolomic clock paradigm, paving the way for a better assessment of berry physiological stage in genetic studies and ultimately for varietal selection and adaptation to climate change.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Flora Tavernier1*, Stefania Savoi2, Laurent Torregrosa3, Philippe Hugueney4, Raymonde Baltenweck4, Vincent Segura1* 5and Charles Romieu1 5

1 UMR AGAP Institute, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34398 Montpellier, France
2 Department of Agricultural, Forest and Food Sciences, Università di Torino (UniTO), 10095 Grugliasco, Italy
3 UMR LEPSE, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34060 Montpellier, France
4 UMR SVQV, INRAe-Colmar, 68000 Colmar, France
5 UMT Geno-Vigne, IFV-INRAe-Institut Agro Montpellier, 34398 Montpellier, France

Contact the author*

Keywords

Vitis vinifera L., untargeted metabolites, single berry, phenology

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

METHYL SALICYLATE, A COMPOUND INVOLVED IN BORDEAUX RED WINES PRODUCED WITHOUT SULFITES ADDITION

Sulfur dioxide (SO₂) is the most commonly used additive during winemaking to protect wine from oxidation and from microorganisms. Thus, since the 18th century, SO₂ was almost systematically present in wines. Recently, wines produced without any addition of SO₂ during all the winemaking process including bottling became more and more popular for consumers. A recent study dedicated to sensory characterization of Bordeaux red wines produced without added SO₂, revealed that such wines were perceived differently from similar wines produced with using SO₂ and were characterized by specific fruity aromas and coolness1,2.

Read More

Evaluation of spraying effects of plant protection unmanned aerial vehicle on two different training systems of vine in Northeast China

In recent years, the application of plant protection unmanned aerial vehicle (UAV) in agricultural pest control has become more and more popular. However, there are few reports about the application of plant protection UAV for wine grapes, and there are no studies comparing the spraying effect of plant protection UAV with that of manual operation in vineyards. In this context, the objective of this study was to explore the feasibility of using plant protection UAV in vineyards instead of manual operations by evaluating the effectiveness of UAV spray in two common grape training systems in Northeast China.

Read More

Key genes in rotundone biosynthesis are affected by temperature, light, water supply, and nitrogen uptake

Rotundone accumulation and biosynthesis is a complicated process. Previous research highlighted that these phenomenons were affected under ecophysiological conditions by viticultural practices (e.g. defoliation or irrigation). Individually, these practices often impact several abiotic factors that are difficult to separate such as temperature, water or nitrogen status, or radiation. Such dissociation can be achieved under controlled environmental conditions using potted vines.

Read More

Exploring the contributions of terroir factors on berry quality of cvs. Cabernet-Sauvignon and Merlot (Vitis vinifera L.) at the Eastern Foothills of the Helan Mountains region of China

Terroir leaves its mark on the accumulation of flavours in grape berries, triggering biochemical re-actions and ultimately shaping wine styles.

Read More

Use of satellite in precision viticulture: the Franciacorta experience

Today, the concept of precision vine management (or site-specific viticulture) has a great relevance. It is based on the practice of a different management in relation to the different features of the crop site. In this way, all practices should be adapted to the land spatial variability and should be linked to the real needs of vines.

Read More