terclim by ICS banner
IVES 9 IVES Conference Series 9 Grape development revisited through the single-berry metabolomic clock paradigm

Grape development revisited through the single-berry metabolomic clock paradigm

Abstract

Although the ripening process of grapevine berries is well-documented at the vineyard level, pinpointing distinct developmental stages remains challenging. The asynchronous development of berries results in dynamic biases and metabolic chimerism. It is thus crucial to consider individual berries separately and resynchronize their internal clock for deciphering physiological changes throughout development. Given the importance of grape composition in wine quality, we aimed at measuring developmental changes in the metabolome of Syrah single berries from anthesis to over-ripening, without a priori preconceived. Non-targeted UHPLC-HRMS analyses of single berries yielded 9,335 compounds with specific mass and retention time. This dataset was submitted to an analysis workflow, combining classification and dimension reduction tools, to reveal the dynamics of metabolite composition. The outcomes of this workflow highlight an innovative redefinition of developmental stages, through the clustering of metabolites into 11 specific kinetic patterns. More precisely, the usual double sigmoidal growth pattern could be split into more transient stages characterized by the accumulation of specific metabolites. For instance, we identified a cluster of metabolites annunciative of the onset of ripening at the end of the herbaceous plateau which witnesses transient lipidic changes. We also found a cluster composed of stilbenes that accumulate during berry shriveling, following sugar loading. This non-targeted approach enables a more precise and unbiased characterization of grapevine berry development through the metabolomic clock paradigm, paving the way for a better assessment of berry physiological stage in genetic studies and ultimately for varietal selection and adaptation to climate change.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Flora Tavernier1*, Stefania Savoi2, Laurent Torregrosa3, Philippe Hugueney4, Raymonde Baltenweck4, Vincent Segura1* 5and Charles Romieu1 5

1 UMR AGAP Institute, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34398 Montpellier, France
2 Department of Agricultural, Forest and Food Sciences, Università di Torino (UniTO), 10095 Grugliasco, Italy
3 UMR LEPSE, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34060 Montpellier, France
4 UMR SVQV, INRAe-Colmar, 68000 Colmar, France
5 UMT Geno-Vigne, IFV-INRAe-Institut Agro Montpellier, 34398 Montpellier, France

Contact the author*

Keywords

Vitis vinifera L., untargeted metabolites, single berry, phenology

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

NEW PLANT BIOPOLYMERS FOR THE COLLOIDAL STABILITY OF THE COLORING MATTER OF RED WINES

The color as well as the “clarity” of red wines are ones of the qualities required by the consumers. Red wines must have colloidal stability from its bottling to its consumption. The supplementation of red wines with additives, and especially Acacia senegal gum, contributes to its organoleptic properties such as the colloidal stabilization of the coloring matter. In a global perspective of limitation of additives in the field of enology, one of the objectives is notably (i) to reduce the use of additives in wines, by their number and/or their quantity, and (ii) to favor the use of natural additives while preserving the organoleptic and sensory qualities of wines.

Heat berry: the influence of abiotic factors on the composition of berries, must and wine in Vitis vinifera L. CV Riesling

Recurring heat and drought episodes during the growing season can produce adverse impacts on grape production in many wine regions around the world.

Effects of post-fermentative cold maceration on chemical and sensory characteristics of Syrah, Cabernet Franc and Montepulciano wines

Astringency sensation decreases slowly during the aging of red wine. Complex reactions of condensation and precipitation of wine polyphenols are involved in this phenomenon. Wine composition and conditions of aging, such as temperature and oxygen availability, strongly influence evolution of the phenol matrix. Recently, a Post-Fermentative cold Maceration (PFM) technique was tested with the aim of accelerating reactions leading to the reduction of astringency and exploiting chemical compounds not extracted from the solid parts of grapes during the previous traditional maceration phase. To this purpose, an innovative maceration system was engineered and used to perform PFM trials on marc derived from vinification of different varieties of red grapes.

Viticultural zoning in D.O.C. Ribeiro (Galicia, NW Spain)

L’AOC Ribeiro est la plus ancienne de Galice (NO de l’Espagne), avec une aire de production potentielle de 3.200 ha. Situé dans la région centrale de la vallée du Miño, le Ribeiro a un climat de tipe maritime tempéré qui se correspond avec la zone climatique II de Winkler.

Yeast Derivatives: A Promising Alternative In Wine Oxidation Prevention?

Oxidation processes constitute a main problem in winemaking. Oxidation result in color browning and varietal aroma loss, which are key attributes of wine organoleptic quality [1]. Despite the mechanisms involved in wine oxidation have been extensively reviewed [2], the protection of wine against oxidative spoilage remains one of the main goals of winemaking.
SO2 is one of the most efficient wine antioxidants used to prevent oxidation and microbial spoilage. However, intolerances caused by SO2 have led to the reduction of its concentration in wines.