terclim by ICS banner
IVES 9 IVES Conference Series 9 Grape development revisited through the single-berry metabolomic clock paradigm

Grape development revisited through the single-berry metabolomic clock paradigm

Abstract

Although the ripening process of grapevine berries is well-documented at the vineyard level, pinpointing distinct developmental stages remains challenging. The asynchronous development of berries results in dynamic biases and metabolic chimerism. It is thus crucial to consider individual berries separately and resynchronize their internal clock for deciphering physiological changes throughout development. Given the importance of grape composition in wine quality, we aimed at measuring developmental changes in the metabolome of Syrah single berries from anthesis to over-ripening, without a priori preconceived. Non-targeted UHPLC-HRMS analyses of single berries yielded 9,335 compounds with specific mass and retention time. This dataset was submitted to an analysis workflow, combining classification and dimension reduction tools, to reveal the dynamics of metabolite composition. The outcomes of this workflow highlight an innovative redefinition of developmental stages, through the clustering of metabolites into 11 specific kinetic patterns. More precisely, the usual double sigmoidal growth pattern could be split into more transient stages characterized by the accumulation of specific metabolites. For instance, we identified a cluster of metabolites annunciative of the onset of ripening at the end of the herbaceous plateau which witnesses transient lipidic changes. We also found a cluster composed of stilbenes that accumulate during berry shriveling, following sugar loading. This non-targeted approach enables a more precise and unbiased characterization of grapevine berry development through the metabolomic clock paradigm, paving the way for a better assessment of berry physiological stage in genetic studies and ultimately for varietal selection and adaptation to climate change.

Download the abstract

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Flora Tavernier1*, Stefania Savoi2, Laurent Torregrosa3, Philippe Hugueney4, Raymonde Baltenweck4, Vincent Segura1* 5and Charles Romieu1 5

1 UMR AGAP Institute, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34398 Montpellier, France
2 Department of Agricultural, Forest and Food Sciences, Università di Torino (UniTO), 10095 Grugliasco, Italy
3 UMR LEPSE, Montpellier University, CIRAD, INRAe, Institut Agro-Montpellier, 34060 Montpellier, France
4 UMR SVQV, INRAe-Colmar, 68000 Colmar, France
5 UMT Geno-Vigne, IFV-INRAe-Institut Agro Montpellier, 34398 Montpellier, France

Contact the author*

Keywords

Vitis vinifera L., untargeted metabolites, single berry, phenology

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Effects of different organic amendments on soil, vine, grape and wine, in a long-term field experiment in Chinon vineyard (France)

In a long-term experiment carried out in Chinon vineyard (37, France) during 23 years, the effects of several organic amendments were studied on soil, vine, grapes and wine. Four main treatments were compared on a calcareous sandy soil: control without organic amendment, dry crushed pruning wood at 2.1.t-1.ha-1.year-1 (D1), cow manure at 10 t-1. ha-1.year-1 (D1) and cow manure applied at 20 t-1.ha-1.year-1 (D2). D1 levels were calculated to fill the annual humus losses by mineralization.

Read More

The performance of grapevines on identified terroirs in Stellenbosch, South Africa

A terroir can be defined as a natural unit that is characterised by a specific agricultural potential, which is imparted by natural environmental features, and is reflected in the characteristics of the final product.

Read More

Marketing terroir wines

The markets for quality wine are becoming more competitive as newer producers emerge and traditional producers improve their quality. The concept of terroir is one way to differenzi­ate wines in a competitive market and to enhance producer income.

Read More

Sensory changes in wines associated with the ripening of Grenache grapes from vineyards in different climatic zones

Climate change is introducing a high variability on grape ripening, causing uncertainty, excessive spending on pesticides and eventually frustrating results in terms of the quality of the vintage, with the increasingly frequent appearance of aromatic problems associated with overripeness, raisining and greenness, which sometimes only appear in bottled wines.

Read More

NACs intra-family hierarchical transcriptional regulatory network orchestrating grape berry ripening

Considering that global warming is changing berry ripening timing and progression, uncovering the molecular mechanisms and identifying key regulators governing berry ripening could provide important tools in maintaining high quality grapes and wine. NAC (NAM/ATAF/CUC) transcription factors represent an interesting family due to their key role in the developmental processes control, such as fruit-ripening-associated genes expression, and in the regulation of multiple stress responses. Between the 74 NAC family members, we selected 12 of them as putative regulators of berry ripening: NAC01, NAC03, NAC05, NAC11, NAC13, NAC17, NAC18, NAC26, NAC33, NAC37, NAC60 and NAC61.

Read More