terclim by ICS banner
IVES 9 IVES Conference Series 9 Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Abstract

Vegetative propagation of grapevines can generate spontaneous somatic variations, providing a valuable source for cultivar improvement. In this context, natural variation in the composition of phenolic compounds in grapevine berries and seeds stands as a pivotal factor in crafting wines with diverse oenological profiles from the same cultivar. To deepen on the understanding of the physiological and genetic mechanisms driving somatic variation in grape phenolics, here we characterized a somatic variant from Tempranillo Tinto, the clone VN21, that exhibits an intense reduced berry skin cuticle and increased extractability of phenolic compounds during wine fermentation. Furthermore, VN21 seeds exhibit anomalous development characterized by diminished lignification, substantial anthocyanin accumulation, and an inherent inability to germinate.

Transcriptomic analysis identified alterations in the phenylpropanoid biosynthesis pathway, outstanding the down-regulation of a secoisolariciresinol dehydrogenase and the up-regulation of a pinorenisol-lariciresinol reductase genes in the berry skin of VN21 compared to the reference Tempranillo Tinto clone RJ43 at veraison stage. These genes encode enzymes in the lignans branch of the phenylpropanoids pathway that are compounds that can potentially reduce the risk of certain cancers and cardiovascular diseases. Ultra-performance liquid chromatography (UPHLC) analysis in both berry skin and seed confirmed a distinct phenylpropanoid accumulation pattern between VN21 and RJ43, with an overall reduction in the accumulation of lignan compounds in VN21.

The results obtained not only contribute to understand grapevine berry development and phenolic composition but also present opportunities for targeted breeding strategies aimed at enhancing desirable traits for wine production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Yolanda Ferradás1,2*, Carolina Royo1, Silvia Yuste1, Pablo Carbonell-Bejerano1, Nuria Mauri1,3, Javier Ibáñez1, María José Motilva1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. De Burgos Km. 6, 26007 Logroño, Spain
2 Current address: Facultade de Bioloxía, Universidade de Santiago de Compostela, 15872 Santiago de Compostela, Spain
3 Current address: Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Cerdanyola del Vallès, Barcelona, Spain

Contact the author*

Keywords

somatic variation, lignans, berry color, UPHLC, RNA-seq

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The temperature‐based grapevine sugar ripeness (GSR) model for adapting a wide range of Vitis vinifera L. cultivars in a changing climate

 Temperatures are increasing due to climate change leading to advances in grapevine phenology and sugar accumulation in grape berries.

Terroir valorization strategies in a reformed denomination area: the Prosecco case study

Aims: This work summarizes some of the upmost recent studies and valorization strategies concerning the Prosecco wine production area. After the geographical denomination Prosecco (DO) was strongly reformed in 2009, the newborn DOCG (controlled and guaranteed DO) and DOC (controlled DO) areas have required different and specific strategies to promote and protect the value of their production.

SENSORY DEFINITION OF A TECHNICAL UNAVOIDABLE TRANSFER OF AROMA COMPOUNDS VIA SEALING IN A BOTTLING LINE IN ORDER TO PREVENT PROSECUTION DUE TO FRAUDULENT AROMATIZATION OF A SUBSEQUENTLY FILLED WINE

In 2020, 12% of all bottled German wines were aromatized, which may increase further due to rising popularity of dealcoholized wines. As sealing polymers of a bottling line absorb aroma compounds and may release them into regular wines in the next filling¹, this unintentional carry-over bears the risk to violate the legal ban of any aromatization of regular wine. However, following EU legislation, German food control authorities accept a technical unavoidable transfer of aroma compounds, if this is of no sensory significance.

Spatial variability of temperature is linked to grape composition variability in the Saint-Emilion winegrowing area

Elevated temperature during the grape maturation period is a major threat for grape quality and thus wine quality. Therefore, characterizing the grape composition response to temperature at a larger scale would represent a crucial step towards adaptation to climate change. In response to changes in temperature, various physiological mechanisms regulate grape composition. Primary and secondary metabolisms are both involved in this response, with well-known effects, for example on anthocyanins, and lesser known effects, for example on aromas or aroma precursors. At the field scale or at the regional scale, however, numerous environmental or plant-specific factors intervene to make the effects of temperature difficult to distinguish from overall variability. In this study, it was attempted to overcome this difficulty by selecting well-characterized situations with differing temperatures.
A long-term study of air temperature variability across several Merlot vineyards in the Saint-Emilion and Pomerol wine producing area found significant temperature differences and gradients at various time scales linked to environmental factors. From this study area, a few sites were selected with similar age, soil and training system conditions, and with repeated and contrasted temperature differences during the maturation period. The average temperature difference during the maturation period was about 2°C between cooler and warmer sites, a difference similar to that expected under future climate change scenarios. In close vicinity to the temperature sensors at each site, grape berries were sampled at different times until full maturity during 2019 and 2020. Also, berries from bunches on either side of the row were analyzed separately, allowing an investigation of bunch exposure effect associated with the coupling of berry temperature and solar radiation. Four replicates of pooled berries for each time – site – bunch exposure combination were obtained and analyzed for biochemical composition. Analyses of variance of the biochemical composition data collected at different sampling times reveal significant effects associated with temperature, site, and bunch azimuth. For instance, anthocyanins in grape skins are clearly influenced by temperature and solar radiation exposure, with up to 30% reduction in warmer conditions.

Adsorption of tetraconazole by organic residues and vineyard organically-amended soils 

Spain is the country with the largest wine-producing area in the EU and its productivity is largely controlled applying fungicides. However, residues of these compounds can move and contaminate surface and groundwater. The objective of this work was to evaluate the capacity of bioadsorbents from different origin to adsorb and immobilize tetraconazole by themselves or when applied as organic soil amendment, and to prevent soil and water contamination by this fungicide. The adsorption of tetraconazole by 3 organic residues: spent mushroom substrate (SMS), green compost (GC) and vine pruning sawdust (VP), as well as by vineyard soils unamended and amended individually with these residues at 1.5% (w/w) was evaluated using the batch equilibrium technique.