terclim by ICS banner
IVES 9 IVES Conference Series 9 Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Abstract

Vegetative propagation of grapevines can generate spontaneous somatic variations, providing a valuable source for cultivar improvement. In this context, natural variation in the composition of phenolic compounds in grapevine berries and seeds stands as a pivotal factor in crafting wines with diverse oenological profiles from the same cultivar. To deepen on the understanding of the physiological and genetic mechanisms driving somatic variation in grape phenolics, here we characterized a somatic variant from Tempranillo Tinto, the clone VN21, that exhibits an intense reduced berry skin cuticle and increased extractability of phenolic compounds during wine fermentation. Furthermore, VN21 seeds exhibit anomalous development characterized by diminished lignification, substantial anthocyanin accumulation, and an inherent inability to germinate.

Transcriptomic analysis identified alterations in the phenylpropanoid biosynthesis pathway, outstanding the down-regulation of a secoisolariciresinol dehydrogenase and the up-regulation of a pinorenisol-lariciresinol reductase genes in the berry skin of VN21 compared to the reference Tempranillo Tinto clone RJ43 at veraison stage. These genes encode enzymes in the lignans branch of the phenylpropanoids pathway that are compounds that can potentially reduce the risk of certain cancers and cardiovascular diseases. Ultra-performance liquid chromatography (UPHLC) analysis in both berry skin and seed confirmed a distinct phenylpropanoid accumulation pattern between VN21 and RJ43, with an overall reduction in the accumulation of lignan compounds in VN21.

The results obtained not only contribute to understand grapevine berry development and phenolic composition but also present opportunities for targeted breeding strategies aimed at enhancing desirable traits for wine production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Yolanda Ferradás1,2*, Carolina Royo1, Silvia Yuste1, Pablo Carbonell-Bejerano1, Nuria Mauri1,3, Javier Ibáñez1, María José Motilva1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. De Burgos Km. 6, 26007 Logroño, Spain
2 Current address: Facultade de Bioloxía, Universidade de Santiago de Compostela, 15872 Santiago de Compostela, Spain
3 Current address: Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Cerdanyola del Vallès, Barcelona, Spain

Contact the author*

Keywords

somatic variation, lignans, berry color, UPHLC, RNA-seq

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The impact of acetaldehyde on phenolic evolution of a free-SO2 red wine

Some wine producers, in good years, can produce free-SO2 red wines and decide to add the minimum amount of sulphur dioxide only at bottling. To manage this addition

Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

The addition of partially dehydrated grapes to enrich must composition for producing complex dry/sweet wines represents a traditional practice in several regions of the world. However, the environmental conditions of dehydration chambers may facilitate the infection of Botrytis cinerea Pers. by promoting disease and provoking large grape losses. B. cinerea attack can induce alterations in the profile of volatile organic compounds (VOCs), which could be detected by sensors specifically trained to detect infection/disease-related compounds. These sensors could facilitate the early detection of the infection, consequently allowing to adjust some dehydration parameters.

Effects of stress memory on grapevine resilience in response to recurrent drought and recovery events 

Plants have evolved different strategies to cope with environmental stresses and, although still debated, it was observed that they can remember past stress occurrence.
Anatomical and physiological adjustments have been observed in different grapevine cultivars after repeated drought exposure, however epigenetic, transcriptional and biochemical changes associated with drought-primed ecological memory have been poorly studied.
This work was conceived to test whether exposure to recurring events of mild drought could prime vines to endure severe drought stress. Particularly, we investigated whether the expected improved stress tolerance of Vitis vinifera cv Nebbiolo plants subjected over years to moderate and long-lasting water stress events (WS-primed) depended on molecular memory phenomena or on resetting of stress-induced signals.

Prove preliminari dl caratterizzazione del vino gutturnio dei colli piacentini

The “GuIturnio dei Colli Piacentini” V.Q.PR.D. results from the vinification of Barbera (55-70%) and Bonarda (30-40%) cultivars, grown in the hilly area of the Piacenza district, identified by the DM 31-07-93 art. 3.
The present work concerns the “zonation” of this area, constituted by 3 valleys Tidone (A), Nure (B) and Arda (C )

Metabolomic profiling of botrytized grape berries: unravelling the dynamic chemical transformations during noble rot

Botrytis cinerea, a fungal pathogen commonly known as grey mold, which under specific climatic conditions can develop into a desirable form known as noble rot. In this process the fungus penetrates the grape skin, allowing water evaporation and concentration of sugars and flavors, while profoundly affects the metabolite composition of grapes, leading to the production of unique and desirable compounds in the resulting wines. The result is a unique and complex wine with a luscious sweetness, heightened aromatics, and a distinct character.