terclim by ICS banner
IVES 9 IVES Conference Series 9 Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Abstract

Vegetative propagation of grapevines can generate spontaneous somatic variations, providing a valuable source for cultivar improvement. In this context, natural variation in the composition of phenolic compounds in grapevine berries and seeds stands as a pivotal factor in crafting wines with diverse oenological profiles from the same cultivar. To deepen on the understanding of the physiological and genetic mechanisms driving somatic variation in grape phenolics, here we characterized a somatic variant from Tempranillo Tinto, the clone VN21, that exhibits an intense reduced berry skin cuticle and increased extractability of phenolic compounds during wine fermentation. Furthermore, VN21 seeds exhibit anomalous development characterized by diminished lignification, substantial anthocyanin accumulation, and an inherent inability to germinate.

Transcriptomic analysis identified alterations in the phenylpropanoid biosynthesis pathway, outstanding the down-regulation of a secoisolariciresinol dehydrogenase and the up-regulation of a pinorenisol-lariciresinol reductase genes in the berry skin of VN21 compared to the reference Tempranillo Tinto clone RJ43 at veraison stage. These genes encode enzymes in the lignans branch of the phenylpropanoids pathway that are compounds that can potentially reduce the risk of certain cancers and cardiovascular diseases. Ultra-performance liquid chromatography (UPHLC) analysis in both berry skin and seed confirmed a distinct phenylpropanoid accumulation pattern between VN21 and RJ43, with an overall reduction in the accumulation of lignan compounds in VN21.

The results obtained not only contribute to understand grapevine berry development and phenolic composition but also present opportunities for targeted breeding strategies aimed at enhancing desirable traits for wine production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Yolanda Ferradás1,2*, Carolina Royo1, Silvia Yuste1, Pablo Carbonell-Bejerano1, Nuria Mauri1,3, Javier Ibáñez1, María José Motilva1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. De Burgos Km. 6, 26007 Logroño, Spain
2 Current address: Facultade de Bioloxía, Universidade de Santiago de Compostela, 15872 Santiago de Compostela, Spain
3 Current address: Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Cerdanyola del Vallès, Barcelona, Spain

Contact the author*

Keywords

somatic variation, lignans, berry color, UPHLC, RNA-seq

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The influence of culture medium on the dynamics of fermentation of wine yeasts

Wine yeast strains Saccharomyces ellipsoideus have important applications in food industry and in this regard is sought isolation as pure cultures and selecting those strains, which in laboratory investigations which have great biotechnological properties This study was intended as the ratio of live cells and autolysates cells also the influence of culture medium on this report. Yeasts selected for this study were isolated from industrial strains of indigenous grape varieties, namely: Feteasca Royal (FR) Feteasca White (FA), black Feteasca (FN), Romanian Tamaioasa (TR), Babeasca Black (BN) and Cotnari Grasa (GC).

High resolution climate spatial analysis of European winegrowing regions

Climate strongly affects the geographical distribution of grape varieties, grapevine cultivation techniques and wine organoleptic properties.

Identification of loci associated with specialised metabolites in Vitis vinifera

Secondary (or specialised) metabolites such as terpenes and phenolic compounds are produced by plants for various roles which include defence against pathogens and herbivores, protection against abiotic stress, and plant signalling. Additionally, these metabolites influence grapevine quality traits such as colour, aroma, taste, and nutritional value. However, the biosynthesis of these metabolites is often complex and controlled by multiple genes which in grapevine are predominantly uncharacterised.

Climate change projections to support the transition to climate-smart viticulture

The Earth’s system is undergoing major changes through a wide range of spatial and temporal scales as a response to growing anthropogenic radiative forcing, which is pushing the whole system far beyond its natural variability. Sources of greenhouse gases largely exceed their sinks, thus leading to a strengthened greenhouse effect. More energy is thereby being supplied to the system, with inevitable shifts in climatic patterns and weather regimes. Over the last decades, these modifications have been manifested in the full statistical distributions of the atmospheric variables, with dramatic changes in the frequency and intensity of extremes. Natural hazards, such as severe droughts, floods, forest fires, or heatwaves, are being triggered by extreme atmospheric events worldwide, thus threatening human activities. Viticultculture is not only exposed to changing climates but is also highly vulnerable, as grapevine phenology and physiological development are strongly controlled by atmospheric conditions. Therefore, the assessment of climate change projections for a given region is critical for climate change adaptation and risk reduction in viticulture. By adopting timely and suitable measures, the future sustainability and resiliency of the sector can be fostered. Climate-grapevine chain modelling is an essential tool for better planning and management. However, the accuracy of the resulting projections is limited by many uncertainties that must be duly taken into account when transferring knowledge to stakeholders and decision-makers. Climate-smart viticulture will comprise ensembles of locally tuned strategies, envisioning both adaptation and mitigation, assisted by emerging technologies and decision-support systems.

Physiological and performance responses of grapevine rootstocks to water deficit and recovery 

Rootstocks play a key role in the grapevine’s adaptation to the increasing soil water scarcity related to climate change. A pot experiment carried out in 2022 aimed at assessing the physiological responses of seven ungrafted rootstocks to a progressive soil water deficit and a subsequent recovery to field capacity.