terclim by ICS banner
IVES 9 IVES Conference Series 9 Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Altered lignans accumulation in a somatic variant of Tempranillo with increased extractability of polyphenols during winemaking

Abstract

Vegetative propagation of grapevines can generate spontaneous somatic variations, providing a valuable source for cultivar improvement. In this context, natural variation in the composition of phenolic compounds in grapevine berries and seeds stands as a pivotal factor in crafting wines with diverse oenological profiles from the same cultivar. To deepen on the understanding of the physiological and genetic mechanisms driving somatic variation in grape phenolics, here we characterized a somatic variant from Tempranillo Tinto, the clone VN21, that exhibits an intense reduced berry skin cuticle and increased extractability of phenolic compounds during wine fermentation. Furthermore, VN21 seeds exhibit anomalous development characterized by diminished lignification, substantial anthocyanin accumulation, and an inherent inability to germinate.

Transcriptomic analysis identified alterations in the phenylpropanoid biosynthesis pathway, outstanding the down-regulation of a secoisolariciresinol dehydrogenase and the up-regulation of a pinorenisol-lariciresinol reductase genes in the berry skin of VN21 compared to the reference Tempranillo Tinto clone RJ43 at veraison stage. These genes encode enzymes in the lignans branch of the phenylpropanoids pathway that are compounds that can potentially reduce the risk of certain cancers and cardiovascular diseases. Ultra-performance liquid chromatography (UPHLC) analysis in both berry skin and seed confirmed a distinct phenylpropanoid accumulation pattern between VN21 and RJ43, with an overall reduction in the accumulation of lignan compounds in VN21.

The results obtained not only contribute to understand grapevine berry development and phenolic composition but also present opportunities for targeted breeding strategies aimed at enhancing desirable traits for wine production.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Yolanda Ferradás1,2*, Carolina Royo1, Silvia Yuste1, Pablo Carbonell-Bejerano1, Nuria Mauri1,3, Javier Ibáñez1, María José Motilva1, José Miguel Martínez-Zapater1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. De Burgos Km. 6, 26007 Logroño, Spain
2 Current address: Facultade de Bioloxía, Universidade de Santiago de Compostela, 15872 Santiago de Compostela, Spain
3 Current address: Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Cerdanyola del Vallès, Barcelona, Spain

Contact the author*

Keywords

somatic variation, lignans, berry color, UPHLC, RNA-seq

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A deep learning object detection approach for smart pest identification in vineyards

Flavescence dorée (FD) poses a significant threat to grapevine health, with the American grapevine leafhopper, Scaphoideus titanus, serving as the primary vector.

Screening of soil yeasts with fermentative capacity from the antarctic continent for their application in the wine industry

AIM: In the last years, many wineries are increasing experimentation to produce more distinguishable beverages. In this sense, the reduction of the fermentation temperature could be a useful tool because it preserves volatile compounds and prevents wines from browning, particularly in the case of white wines.

Comparing different vineyard sampling densities and patterns for spatial interpolation of intrinsic water use efficiency

The need to rationalize agricultural inputs has recently increased interest in assessing vineyard variability in order to implement variable rate input applications, so-called ‘precision viticulture’. In many viticultural areas globally, precision viticulture is already widely used such as for selective harvesting and variable rate application (VRA) of inputs such as irrigation and/or fertilizer. Robust VRA relies on having a geostatistically accurate map (of one or more vineyard attributes) requiring high sampling densities, which can be cost- and time-prohibitive to obtain. Previous work on spatial interpolation using kriging have upscaled ground-based measurements, but such upscaling strategies are applicable only when vineyard conditions are spatially continuous and satisfies the assumption of second-order stationary processes. Alternatively, mixed models that combine kriging and auxiliary information, such as the regression kriging (RK) method, are more instructive for spatial predictions. In order to improve prediction accuracies, it is therefore necessary to incorporate additional information to achieve accurate spatial patterns with low error.

Stability of 3-mercaptohexanol during white wine storage in relationship to must pre-fermentative fining

3-Mercaptohexanol (3MH) is a volatile thiol occurring in several white and red wines, where it can contribute to fruity attributes. Its content is typically high in wines from certain grape varieties, in particular Sauvignon blanc, where it is considered a varietal marker. The strong nucleophilic character of thiols makes 3MH rather unstable during wine storage, due to the presence of several strong electrophilic species. Among these electrophilics, those arising from the oxidation of flavan3-ols such as catechin and epi-catechin have been indicated as critical for 3MH stability. Accordingly, there is a generalized interest towards the ability of vinification practices to reduce 3MH loss during aging through the management of wine flavan-3-ols content.

Short-term relationships between climate and grapevine trunk diseases in southern French vineyards

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...