terclim by ICS banner
IVES 9 IVES Conference Series 9 The sensitivity to ABA affects the cross-talk between scion/rootstock in tolerant grapevines to drought stress

The sensitivity to ABA affects the cross-talk between scion/rootstock in tolerant grapevines to drought stress

Abstract

Drought caused by climate change has a dramatic incidence on the vineyard. Despite employing specific rootstocks tolerant to drought like 110 Richter, the vineyard continues to experience various losses, revealing the importance of the scion cultivar in the adaptation to drought stress. In this regard, Merlot, a widely cultivated grapevine, exhibited reduced drought tolerance compared to less cultivated varieties like Callet, a local cultivar originating from the Balearic Islands that demonstrated greater resilience to drought. Therefore, understanding the drought stress response in both cultivars and the cross-talk between scion and rootstock is key to unveiling possible differences that could affect to the adaptation to drought in vineyard. Plants from both cultivars grafted in the tolerant rootstock, 110 Richter, underwent different drought stages. For each stage, samples from leaves and roots were analyzed at metabolic, hormonal, physiological and transcriptomic level. The results revealed differences at most levels, increasing the production of osmolytes and antioxidant molecules involved in response to drought stress in Callet. However, hormonal analysis showed similar ABA production in both cultivars, indicating lower sensitivity to ABA in the case of Merlot compared to Callet. Moreover, the transcriptomic analysis revealed a modulation of genes involved in response to ABA and miRNA in leaves and roots of Callet, whereas in Merlot was mostly absent in roots, evidencing a poor cross-talk between Merlot and rootstock and increasing the value of the correct combination scion/rootstock for the vineyard adaptation to climate change.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Alberto Rodriguez-Izquierdo1*, David Carrasco1, María Ángeles Revilla2, Josefina Bota3, Rosa Arroyo-Garcia1

1 Centro de Biotecnología y Genómica de Plantas (CBGP-INIA), CSIC – Universidad Politécnica de Madrid, Campus Montegancedo, Madrid, Spain
2 Department of Organisms and Systems Biology, Institute of Biotechnology of Asturias, University of Oviedo, Oviedo, Spain
3 Research Group on Plant Biology under Mediterranean Conditions, Departament de Biologia, Universitat de les Illes Balears (UIB) – Agro-Environmental and Water Economics Institute (INAGEA). Carretera de Valldemossa Km 7.5, 07122 Palma, Illes Balears, Spain

In memoriam of Rosa Arroyo-Garcia.

Contact the author*

Keywords

rootstock, drought, cross-talk, transcriptomics, ABA

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Photodegradation of retsina wine: does pine resin protect against light-induced changes?

Retsina is a wine deeply rooted in Greek tradition but often misunderstood, largely due to the poor quality associated with past production. Historically, pine resin was used to seal wine transport containers, and over time, its distinctive aroma led to its intentional incorporation into winemaking.

Use of a recombinant protein (Harpin αβ) as a tool to improve phenolic composition in wines

Climate change is modifying environmental conditions in all wine-growing areas of the
world.

Spatial variability of temperature is linked to grape composition variability in the Saint-Emilion winegrowing area

Elevated temperature during the grape maturation period is a major threat for grape quality and thus wine quality. Therefore, characterizing the grape composition response to temperature at a larger scale would represent a crucial step towards adaptation to climate change. In response to changes in temperature, various physiological mechanisms regulate grape composition. Primary and secondary metabolisms are both involved in this response, with well-known effects, for example on anthocyanins, and lesser known effects, for example on aromas or aroma precursors. At the field scale or at the regional scale, however, numerous environmental or plant-specific factors intervene to make the effects of temperature difficult to distinguish from overall variability. In this study, it was attempted to overcome this difficulty by selecting well-characterized situations with differing temperatures.
A long-term study of air temperature variability across several Merlot vineyards in the Saint-Emilion and Pomerol wine producing area found significant temperature differences and gradients at various time scales linked to environmental factors. From this study area, a few sites were selected with similar age, soil and training system conditions, and with repeated and contrasted temperature differences during the maturation period. The average temperature difference during the maturation period was about 2°C between cooler and warmer sites, a difference similar to that expected under future climate change scenarios. In close vicinity to the temperature sensors at each site, grape berries were sampled at different times until full maturity during 2019 and 2020. Also, berries from bunches on either side of the row were analyzed separately, allowing an investigation of bunch exposure effect associated with the coupling of berry temperature and solar radiation. Four replicates of pooled berries for each time – site – bunch exposure combination were obtained and analyzed for biochemical composition. Analyses of variance of the biochemical composition data collected at different sampling times reveal significant effects associated with temperature, site, and bunch azimuth. For instance, anthocyanins in grape skins are clearly influenced by temperature and solar radiation exposure, with up to 30% reduction in warmer conditions.

Biomass carbon and nitrogen input from cover crops in an irrigated vineyard in Okanagan Valley, Canada

The use of cover crops in vineyards has been encouraged by positive effects on wine grape yield and sensory attributes, and improved soil function. This study examined the efficacy of three alleyway and three undervine cover crop treatments in an organic vineyard in the semiarid Okanagan Valley, Canada in 2021.